• Journal of Microbiology
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• 제37권4호
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• pp.206-212
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• 1999

In order to isolate a Fe(III)-reducer from the natural environment, soil samples were collected from various patty fields and enriched with ferric citrate as a source of Fe(III) under anaerobic condition. Since the enrichment culture was serially performed, the Fe(III)-reduction activity was serially diluted and cultivated on an agar plate containing lactate and ferric citrate in an anaerobic glove box. A Gram negative, motile, rod-shaped and facultative anaerobic Fe(III)-reducer was isolated based on its highest Fe(III)-reduction activity, Bacterial growth was coupled with oxidation of lactate to Fe(III)-reduction, but the isolate fermented pyruvate without Fe(III), The isolate reduced an insoluble ferric iron (FeOOH) as well as a soluble ferric iron (ferric citrate). Using the BBL crystal enteric/non-fermentor identification kit and 16S rDNA sequence analysis, the isolate was identified as Shewanella putrefaciens IR-1.

• 미생물학회지
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• 제39권3호
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• pp.175-180
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• 2003

Shewanella putrefaciens DK-1은 그람음성, 통성 혐기성 세균으로 $NO_{3}$, Fe(III), Mn(IV), humic acid와 같은 다양한 전자수용체를 이용한다. S. putrefaciens DK-1의 전자공여체의 이용능력은 제한적이며, lactate나 formate는 좋은 전자공여체로 이용되지만 acetate나 toluene은 이용하지 못하였다. 다양한 전자수용체간의 경쟁을 살펴보기 위해 전자수용체로 Fe(III)와 같이 $NO_{3}^{-}$, $NO_{2}^{-}$를 넣어 주었을 매 Fe(III)의 환원은 저해되었다. 또한 5. putrefaciens DK-1은 전자수용체로 토양에 광범위하게 존재하는 humic acid를 이용하였으며, 환원된 humic acid는 질산염에 의해서 다시 산화되었다. Fe(III) 환원능이 있는 환경 시료를 이용하여 탄소, 질소, 인과 같은 제한 요인이 Fe(III) 환원세균의 활성에 미치는 효과를 조사하였다. 천호지의 저질토와 대호의 농토에 각각 탄소원, 질소원, 인을 첨가해 주었을 경우 S. putrefaciens DK-1과 탄소원을 동시에 첨가해 주었을 때 가장 높은 철 환원능을 보여주었다.

• Environmental Analysis Health and Toxicology
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• 제15권1_2호
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• pp.7-12
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• 2000

Scavenging effects on paraquat induced toxicity were investigated by using methanol (MeOH) and ethylacetate (EtoAC) extracts of Lonicera japonica. The results are summerized as follows: 1. To Fe(III)-ADP-NADPH induced microsomal lipid peroixdation, MeOH and EtoAC extracts showed antioxidative activiies and inhibition ratio at 100 $\mu\textrm{g}$/$m\ell$ 44.4% and 73.8% respectively 2. To microsomal NADPH dependent cytochrome p -450 reductase in rat liver, MeOH and EtoAC extracts inhibited the enzyme activiies and inhibition ratio were 26.3% and 44.8% respectively. 3. Administration (30 mg/kg, iv) of paraquat to rats caused the marked elevation of GOT, GPT, LDH, ALP in the serum and lipid peroxides in the microsome as compared to the control group. Serum GTP, LDH, ALP and liver microsomal LPO were reduced significantaly by administration of MeOH extract. (1,000 mg/kg), EtoAC extract (40 mg/kg) and Silymarin (150 mg/kg) as compared to the paraquat group. From the results, MeOH and EtoAc exuacts. of Lonicera japonica showed the useful scavenger and reducer on the paraquat induced hepatotoxicty.