• Title/Summary/Keyword: Fatty Acids Composition

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A Study on the Cellular Fatty Acid Profiles of Listeria spp. Isolated from Foods (일반식품에서 분리된 Listeria spp.의 지방산 조성에 관한 연구)

  • 이명숙;김미은;이원재;김진상;이훈구;강지희
    • Journal of Food Hygiene and Safety
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    • v.11 no.2
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    • pp.107-114
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    • 1996
  • The distribution of Listeria spp. in various foods and its fatty acid composition were examined. A total 60 samples of dairy products(15), seafoods(20), meat products(18), factory waster(2), and salades(5) were tested. Listeria spp. was found 10 samples, showing about 16.7% detection ratio; dairy products 0(0%),,seafoods 1(5%), meat product 7(38.9%), and factory wastes 2(100%). Whereas L. welshimeri was isolated from meat products 1(5.6%) and factory wastes 1(50%). The cellular fatty acid composition determined by gas chromatography was found not to differ among L. innocua isolated from food has similar fatty acid profiles when grown at 3$0^{\circ}C$,24 hrs on the tryptic soy plate with C15 and C17 anteiso branched acids accounting for about 80% of total.

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The Effects of Carbon Sources on the Biosynthesis of the Phospholipid and the Fatty Acid Composition of Mitochondria in Chlorella ellipsoidea

  • Yoon, Seung-Hee;Jang, Jae-Seon;Lee, Chong-Sam
    • BMB Reports
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    • v.29 no.4
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    • pp.343-352
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    • 1996
  • The biosynthesis of phospholipid and the composition of fatty acid were analyzed in mitochondria isolated from Chlorella ellipsoidea treated with carbon sources (glucose, sucrose, raffinose) during the culture. The growth of Chlorella and total lipid contents in mitochondria treated with various carbon sources was increased to compare with the control. When Chlorella mitochondria was treated with various carbon sources, four kinds of phospholipid were increased predominantly. The major fatty acids utilized for the biosynthesis of the phospholipid were analyzed linoleic acid (average 25.18%) and stearic acid (average 10.52%) in the control. But, it was shown that the major fatty acids in Chlorella mitochondria treated with glucose were stearic acid (average 30.93%), palmitic acid (average 17.47%) and stearic acid (average 20.31%), linoleic acid (average 16.68%) in sucrose treatment and oleic acid (average 17.17%), palmitic acid (average 15.64%) in raffinose treatment.

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Studies on Lipids and Proteins of Rabbit Meat -I. Emphasis on lipid component of rabbit meat- (토끼고기의 지방질과 단백질에 관한 연구 -I. 지방성분을 중심으로-)

  • Leekim, Yang-Cha;Ahn, Hong-Seok
    • Journal of Nutrition and Health
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    • v.10 no.2
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    • pp.18-26
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    • 1977
  • Among animals, the rabbit is known to be affected most sensitively by dietary changes and to be most susceptible to atherosclerosis. The exact reason is still unknown as to whether the primary cause is intrinsic (tissue itself) or extrinsic such as a blood factor which could be influenced by various dietary means. It is of utmost importance to check the nutritional quality of rabbit meat before it is accepted and adapted as a daily food item. To evaluate nutritional quality of rabbit meat, studies on various aspects of lipid components were carried out in comparison with other animal meats such as beef, pork and chicken also included in the study was the question whether the cooking and storage conditions influence the composition of fatty acids and cholesterol level. Some results and findings are listed below: 1. The content of linoleic acid (18 : 2), one of the essential fatty acids, was much higher in rabbit meat compared to the other meats. The Percentages of this Polyunsaturated fatty acids, was much higher in rabbit meat compared to the other meats. The percentages of this polyunsaturated fatty acid in terms of total fatty acids were $37.3{\pm}3.7$, 5.9, 14.5, and 21.9% for rabbit, beef, pork, and chicken respectively. The degree of unsaturation was high not only in meat but also in liver and adipose tissue of rabbit. The values of iodine number, the indication of degree of unsaturation, were known to be $102{\sim}107$, $32{\sim}47$, $46{\sim}67$, and $55{\sim}77$ for rabbit, beef, pork, and chicken respectively. Such a high proportion of this polyunsaturated fatty acid contained in rabbit meat could be harmful due to their Peroxidation effect. 2. A small amount of lower (short chain) fatty acids was isolated from rabbit tussues, which were not observed in other animal's tissues. The significance of this small amount of short chain fatty acids contained in rabbit meat remained an open question. 3. The concentration of total cholesterol in rabbit meat was similar to that of otherr but the content of esterified cholesterol was higher in rabbit meat. This was probably due to the perference of cholesterol to esterify with unsaturated fatty acids. By roasting the percentage of Polyunsaturated fatty acids was decreased while saturated palmitic acid was proportionally increased. 4 The composition of fatty acids were affected more by dry heat than moist heat. More research should be pursued to improve methods of preservation and storage to prevent possible peroxidation and rancidity problems of rabbit meat. In the meantime, the public should be informed to eat fresh rabbit meat and not to store it for a long period of time. This study was supported by the Ministry of Science and Technology in Korea.

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STUDIES ON THE LIPID OF AQUATIC PRODUCTS (PART 3) Fatty Acid Composition the Lipid in Dried Purple Laver Marketable Products (수산물의 지질에 관한 연구(제3보) -산지별 건해태 지질의 지방산조성에 대하여-)

  • HA Bong Seuk
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.219-225
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    • 1978
  • Several samples of tile dried purple laver grown and processed at the major laver producing districts, i. e Mokpo, Wando, Hadong and Jangrim, along the southern coast of Korea were Quantitatively investigated to determine composing patterns of the fatty acids by gas-liquid chromatography. The total lipid contents in dried purple laver of Hadong were $1.8\%$ being the highest value. Upon analyzing fatty acid composition, some differences were observed in their quantitative distribution at different growing places. Dried purple laver of Wando contained mainly $C_{16:0},\;C_{18:1},\;C_{16:1}\;and\;C_{17:0}$ fatty acids, that of Mokpo contained $C_{16:0},\;C_{20:5},\;C_{18:1}\;and\;C_{16:1}$ fatty acids, and that of Hadong $C_{16:0},\;C_{20:5},\;C_{18:0}\;and\;C_{20:1}$ fatty acids, and that of Jangrim $C_{20:5},\;C_{16:0},\;C_{18:1}\;and\;C_{18:0}$ fatty acids, each in order of acid quantity. In regard to the composing pattern of carbon number of fatty acids, the dried purple laver o Mokpo, Hadong, and Jangrim contained $C_{16},\;C_{18}\;and\;C_{20}$ fatty acids with the identical distributional pattern, while that of Jangrim contained $C_{16},\;C_{18}\;and\;C_{17}$ fatty acids as major components. Dried Purple laver of Jangrim contained especially high amount of $C_{20:5}$ fatty acids.

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Comparison of Lipid Components Between Wild and Culture Sea Mussels (천연산과 양식산 담치의 지질성분)

  • 류병호
    • Journal of Nutrition and Health
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    • v.19 no.3
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    • pp.199-203
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    • 1986
  • The Lipids content of sea mussels was studied and the composition of neutral lipids, glycolipids, and phospholipids were analyzed for the sea mussels obtained from Pusan area in Korea. Fatty acids were found in roder of neutral lipid, phospholipid and glycolipid. total lipids and fatty acids were appeared higher in Mytilus edulis then Mytilus cosuscus. The neutral lipids showed to be approximately 50% of 새심 lipids in Mytilus coruscus and mytilus edulis. The phospholipids showed no significant differences between male and female of Mytilus edulis. The major fatty acids were consisted of C 16 : 0, C16 : 1, C18 ; 1 and C22: 6 in order. The level of C16 :0 with saturated acid was highest in lipids. The level of C18 :1 . C18 :2, C22 : 6 were higher in Mutilus edulis than Mytilus coruscus. $\omega$$_3$ High unsaturated fatty acid essential fatty acids C20 : 5, C22 ; 6 were higher in Mytilus edulis than Mytilus coruscus.

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Oxidative Stability of Fatty Acids and Tocopherols in the Fats and Oils during Microwave Heating (Microwave 오븐 가열에 의한 유지의 지방산과 토코페롤의 안정성)

  • 주광지;김은미
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.24 no.2
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    • pp.234-241
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    • 1995
  • Effect of microwave heating on the oxidative stability of the soybean oil, sesame oil, butter and margarine were investigated by mearsuring fatty acids amout and tocopherol losses. The index for chemical properteis, free fatty acid, peroxide value, anisidine value, carbonyl value, conjugated diene and triene levels were also mearsured in the oil samples for 5, 10, 15 and 20 min of heating in a microwave oven. No significant difference was observed on the fatty acids composition in the fats and oils before and after microwave heating. During microwave treatment, the oxidative degradation of the tocopherols in the samples became greater with increasing heating time. The amount of tocopherols in the soild fats, butter and margarine, dropped drastically after 5 min of heating and reduced to 95% of their original levels after 20min heating ${\gamma}$-tocopherol in butter showed the most unstable states and completely destroyed during microwave treatment for 20min. On the other hand, 80% of tocopherols in the liquid oils were still remained after 5min of heating except $\delta$-tocopherol in sesame oil.

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Effects of Perilla and Corn Oil Diets on the Hepatic Microsomal Fatty Acid Composition, Cytochrome P-450 Contents, and Glutathione S-transferase Activities in 2-Acctylaminofluorcne Treated Rats (들깨유 옥수수유의 섭취가 2-Acetylaminofluorene을 투여한 쥐 간에서 소포체막의 지방산 조성과 Cytochrome P-450 함량, Glutathione S-transferase 활성도에 미치는 영향)

  • 김경민
    • Journal of Nutrition and Health
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    • v.25 no.1
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    • pp.3-11
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    • 1992
  • This paper examines the effects of dietary fats on the fatty acid composition and market enzyme activites during liver damage in 2-acetylaminofluorene treated rats. Weaning Sprague-Dawley male rats were fed the diet of beef tallow(BT source of sturated fatty acid) corn oil(CO source of n-6 fatty acid) and perilla oil(PO source of n-3 fatty acid) at the level of 15% fat. Ten days after feeding 2-acetylaminofluorene(2-AAF) was injected intraperitoneally twice every week at the level of 50mg/kg body weight for 7 weeks. Liver microsomal and cytosolic fractions were collected to determine the microsomal fatty acid composition lipid peroxide(malondialdehyde MDA) contents glucose 6-phosphatase(G6 Pase) activity cytochrome(Cyt) P-450 contents and cytosolic glutathione S-transferase(G6 Pase) activity cytochrome(Cyt) P-450 contents and cytosolic glutathione S-transferase(GST) activity. The fatty acid composition in microsomal fraction was reflected by different dietary fats. By 2-AAF treatment linoleic acids were increased regardless of the diet MDA contents were higher in CO group than it was in BT group. However 2-AAF treatment decreased MDA contents in all dietary groups. G6Pase activity of BT group was higher than those of the other gropus. CO group had the highest Cyt P-450 contents and 2-AAF treatment lowered Cyt P-450 contents only in CO gropu GST activites were higher in CO than in BT group whereas the enzyme activites were increased by 20AAF treatment in all dietary groups. These results suggest that dietary fats and 2-AAF treatment in all dietary groups,. These results suggest that dietary fats and 2-AAF treatment affect microsomal fatty acid composition The enzyme activities concerned with liver damage were influenced differently by dietary fats and 2-AFF treatment Although PO diet contains much more polyunsaturated fatty acids than CO diet PO diet doesn't cause more oxidant stress compared with CO diet in these data.

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Effect of Supplementation of Fish and Canola Oil in the Diet on Milk Fatty Acid Composition in Early Lactating Holstein Cows

  • Vafa, Toktam S.;Naserian, Abbas A.;Moussavi, Ali R. Heravi;Valizadeh, Reza;Mesgaran, Mohsen Danesh
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.3
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    • pp.311-319
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    • 2012
  • This study examined the effects of supplementation of fish oil and canola oil in the diet on milk yield, milk components and fatty acid composition of Holstein dairy cows in early lactation. Eight multiparous early lactation Holstein cows ($42{\pm}12$ DIM, $40{\pm}6kg$ daily milk yield) were fed a total mixed ration supplemented with either 0% oil (Control), 2% fish oil (FO), 1% canola oil +1% fish oil (FOCO), or 2% canola oil (CO) according to a double $4{\times}4$ Latin square design. Each period lasted 3 wk; experimental analyses were restricted to the last week of each period. Supplemental oils were added to a basal diet which was formulated according to NRC (2001) and consisted of 20% alfalfa, 20% corn silage and 60% concentrate. Milk yield was similar between diets (p>0.05), but dry matter intake (DMI) was lower (p<0.05) in cows fed FO diet compared to other diets. Milk fat percentage and daily yield decreased (p<0.01) with the supplementation of fish and canola oil. The daily yield and percentage of milk protein, lactose and solids-not-fat (SNF) were not affected by diets (p>0.05). The proportion (g/100 g fatty acids) of short chain fatty acids (SCFA) decreased and polyunsaturated fatty acids (PUFA) increased (p<0.05) in milk of all cows fed diets supplemented with oil. The proportions of 6:0, 8:0, 10:0 12:0 and 14:0 fatty acids in milk fat decreased (p<0.01) for all diets supplemented with oil, but the proportions of 14:1, 16:0 and 16:1 fatty acids were not affected by diets (p>0.05). The proportion of trans(t)-18:1 increased (p<0.01) in milk fat of cows fed FO and FOCO diets, but CO diet had the highest proportion of cis(c)-11 18:1 (p<0.01). The concentration of t-10, c-12 18:2, c-9 t-11 18:2, 18:3, eicosapentaenoic acid (EPA, 20:5) and docosahexaenoic acid (DHA, 22:6) increased (p<0.05) in FO and FOCO diets in comparison with the other two diets. These data indicate that including fish oil in combination with canola oil significantly modifies the fatty acid composition of milk.

Solid Phase Extraction of Phospholipids from Brazil Nut (Bertholletia excelsa) and Their Characterization by Mass Spectrometry Analysis

  • Lima, Bruna R. De;Silva, Felipe M.A. Da;Koolen, Hector H.F.;Almeida, Richardson A. De;Souza, Afonso D.L. De
    • Mass Spectrometry Letters
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    • v.5 no.4
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    • pp.115-119
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    • 2014
  • The Brazil nut (Bertholletia excelsa - Lecythidaceae) is considered a product with high economic value, being a food widely appreciated for its nutritional qualities. Although previous studies have reported the biochemical composition of Brazil nut oil, the knowledge regarding the phospholipid composition exhibits a disagreement: the composition of fatty acids present in the structures of phospholipids is reported as being different from the composition of the free fatty acids present in the oil. In this work, solid phase extraction (SPE) was employed to provide a fast extraction of the phospholipids from Brazil nuts, in order to compare the phospholipid profile of the in nature nuts and their fatty acids precursor present in the oil. The major phospholipids were characterized by mass spectrometry approach. Their fragmentation pattern through direct infusion electrospray ionization ion-trap tandem mass spectrometry ($ESI-IT-MS^2$) proved to be useful to unequivocal characterization of these substances. High resolution (HR) experiments through ESI using a quadruple time of flight mass spectrometry (QTOF) system were performed to reinforce the identifications.

Fatty Acid Composition and Content of Omega-3 Polyunsaturated Fatty Acids of Major Fishes Caught in Korean Seas (한국 주요 어종의 지방산 조성 및 ${\omega}-3$ 고도 불포화 지방산의 함량)

  • Ahn, Byung-Hak;Shin, Hyun-Kyung
    • Korean Journal of Food Science and Technology
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    • v.19 no.3
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    • pp.181-187
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    • 1987
  • The lipids of ten major fishes caught in Korean seas were analyzed for fatty acid composition and for the content of $\omega-3$ polyunsaturated fatty acids. The major fatty acids present were hexadecanoic acid $(14{\sim}24%)$, hexadecenoic acid $(4{\sim}13%)$, octadecenoic acid $(8{\sim}29%)$, eicospentaenoic acid $(4{\sim}18%)$ and docosahexenoic acid $(6{\sim}33%)$. The ${\omega}-3$ polyunsturated fatty acids ranged from 18% to 48% of the total fatty acids depending on the species. The content of eicosapentaenoic acid was relatively high in sardine, gizzard shad and atka fish on the basic of fresh body weight, while that of docosahexaenoic acid was highest in hair tail and followed by atka fish and sardine.

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