• Asian-Australasian Journal of Animal Sciences
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• v.13 no.9
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• pp.1235-1238
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• 2000

Thirty-six Holstein dairy cows were used to evaluate the effect of a rumen protected methionine supplement (RPMet). The cows were divided into two groups of 18 each (control/experimental). The experimental group was given 15 g/d of RPMet (Mepron $^{(R)}$M85, Degussa) from the 4th to the 26th week postpartum. All cows were fed a similar amount of forage including alfalfa silage, corn silage and timothy silage. Concentrate mixture was offered in proportion to the milk yield of each cow. Sufficiency of major metabolizable AAs was checked. Milk yield and milk composition was monitored for each individual cow. A metabolic profile test (MPT) was carried out at the 7th, 11th and 21st week postpartum. Without supplement, both methionine and leucine fell short of the daily requirement. Supplementation with 15 g/d RPMet was calculated to be within a sufficient margin of safety. Milk yield tended to remain higher in the supplemented group than in the controls during supplementation with RPMet. The differences in weekly milk production at the 17th, 18th, 19th and 22nd weeks postpartum were significantly high in the RPMet group (p<0.05). The average 305-d milk yield and the percentages of milk fat, milk protein and solids-not-fat were not affected by the treatment. No differences were observed in either the somatic cell count in the milk or the reproductive status. Judging from MPT, all the cows were in good health during lactation.

• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.237-248
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• 1992

The effect of 16 different dietary rations, computed by the combinations of 13, 16, 19 or 22% CP and 2600, 2800, 3000 or 3100 kcal ME/kg, on growth performances and carcass yield of Starcross layers were assessed in two similar experiments. In both experiments, the body weight, eviscerated carcass yield, edible carcass yield, length of digestive tract and shank length increased but the feed intake decreased linearly with the increase of dietary CP and ME levels. The liver and gizzard weights as percentages of live weight tended to be increased with the increase of dietary CP and ME levels. The carcass dry matter, crude protein, fat, ash and energy content were not influenced by the dietary CP and ME levels. Dietary CP levels had positive correlations with all the parameters (except feed and energy intake and carcass dry matter). However, the dietary ME levels were positively correlated with all the parameters (except feed and energy intake; carcass dry matter and ash) in both experiments. The higher values were noted for all the parameters (except gizzard and carcass fat percentages) studied in Experiment 1 compared to those observed in Experiment 2.

• Animal Bioscience
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• v.35 no.1
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• pp.147-152
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• 2022

Objective: This study was conducted to obtain an objective index that can be quantified and used for establishing an animal welfare certification standard in Korea. For this purpose rumen pH, ruminating time, milk yield, milk quality, and blood components of cows reared in farms feeding high forage level (90%) and farms feeding low forage level (40%) were compared. Methods: Data on rumen pH, rumination time, milk yield, milk fat ratio, milk protein ratio, and blood metabolism were collected from 12 heads from a welfare farm (forage rate 88.5%) and 13 heads from a conventional farm (forage rate 34.5%) for three days in October 2019. Results: The rumination time was longer in cattle on the welfare farm than on the conventional farm (p<0.01), but ruminal pH fluctuation was greater in the cattle on conventional farm than the welfare farm (p<0.01). Conventional farms with a high ratio of concentrated feed were higher in average daily milk yield than welfare farms, but milk fat and milk production efficiency (milk fat and milk protein corrected milk/total digestible nutrients) was higher in cattle on welfare farms. Blood test results showed a normal range for both farm types, but concentrations of total cholesterol and non-esterified fatty acid were significantly higher in cows from conventional farms with a high milk yield (p<0.01). Conclusion: The results of this study confirmed that cows on the animal welfare farm with a high percentage of grass feed had higher milk production efficiency with healthier rumen pH and blood metabolism parameters compared to those on the conventional farm.

• Food Science of Animal Resources
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• v.37 no.3
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• pp.376-384
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• 2017

The objective of this study was to investigate the effect of using gelled emulsion (olive oil 46%, inulin 9%, gelatin 3%) as fat replacer on some quality parameters of chicken patties. For this purpose GE, prepared with olive oil, gelatin and inulin was replaced with beef fat at a level of 0%, 25%, 50%, 100% (C, G25, G50, G100). In this study syneresis, thermal stability, centrifuge and creaming stability of gelled emulsion were analyzed. Chemical composition, technological paramerers (cooking yield, water holding capacity, diameter reduction, fat and moisture retention) and textural and sensory properites were evaluated in comparision to control patties. High thermal stability was recorded in GE (93%), also creaming stability results showed that GE protected its stability without any turbidity and separation of the layer. The complete replacement of beef fat with GE showed detrimental effect on all investigated cooking characteristics except fat retention. Replacement of beef fat with GE at a level of 50% resulted similar cooking characteristics with C samples. Color parameters of samples were affected by GE addition, higher CIE $b^*$ values observed with respect to GE concentration. The presence of GE significantly affected textural behaviors of samples (p<0.05). Our results showed that GE prepared with inulin and olive oil is a viable fat replacer for the manufacture of chicken patty.

• Food Science of Animal Resources
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• v.34 no.4
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• pp.415-422
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• 2014

Duck feet gelatin (DFG) gel was added as a fat replacer to low-fat frankfurters and the effect of DFG on physicochemical, textural, and sensory characteristics of low-fat frankfurters was evaluated. DFG gel was prepared with a 20% duck feet gelatin concentration (w/w). Adding DFG decreased lightness and increased yellowness of the low-fat frankfurters (p<0.05). However, DFG did not affect redness of low-fat frankfurters (p>0.05). The statistical results indicated that adding DFG improved cooking yield of low-fat frankfurters (p<0.05). In addition, replacing pork back fat with DFG resulted in increased moisture content, protein content, and ash content of low-fat frankfurters, and the low-fat frankfurter formulated with 5% pork back fat and 15% DFG gel had the highest moisture content and lowest fat content (p<0.05). Adding of DFG increased all textural parameters including hardness, springiness, cohesiveness, chewiness, and gumminess of low-fat frankfurters (p<0.05). In terms of sensory properties, the low-fat frankfurter formulated with 5% pork back fat and 15% DFG gel showed similar satisfaction scores for the flavor, tenderness, juiciness, and overall acceptance when compared to the regular frankfurters (20% back fat). Therefore, our results suggest that DFG could be an effective novel source, as a fat replacer, for manufacturing of low-fat frankfurters.

• Food Science and Preservation
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• v.30 no.5
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• pp.758-769
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• 2023

The main aim of this study was to determine python fat's several physicochemical properties, including dimensions, color, structure, acid value (AV), saponification value (SV), density, and recovery efficiency. The optimum yield obtained was approximately 80.40% at 180℃ for 60 min with an AV of 0.3366 and SV of 179.56 mg KOH/g. Fatty acids, comprising oleic acid (72.462%), palmitic acid (26.243%), linolenic acid (0.835%), and myristic acid (0.459%), were identified using gas chromatography-mass spectrometry (GC-MS). The python fat had a very weak antioxidant capacity and almost no antibacterial ability with gram-positive (Staphylococcus aureus - ATCC 25923 and Bacillus cereus - ATCC 10876) and gram-negative (Escherichia coli - ATCC 25922 and Salmonella enterica - ATCC 35664) bacteria (used the paper disc diffusion method for antibiotic susceptibility testing). Moreover, python fat is considered to be very resistant to high temperatures.

• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.327-334
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• 2003

This study was carried out to make early prediction of carcass yield grade. Sixty six Hanwoo steers were measured for back fat thickness, longissimus muscle area and body weight at 18, 21 and 24 months of age by ultrasound. Carcass evaluation was done after ultrasound measurement at 24 month of age. Ultrasonic yield grade at 18, 21 and 24 month of age were predicted by regression and decision tree methods. Classifying by carcass yield grade, ultrasonic back fat thickness at 18, 21 and 24 months of age was significantly different in each carcass yield grade (p<0.05). The prediction accuracy of carcass yield grade by regression method was 78.8% at 18 months, 86.4% at 21 months and 90.9% at 24 months of age. By using the decision tree method for carcass yield grade, 78.8%, 89.4% and 89.4% of prediction accuracy were obtained at 18, 21 and 24 months of age, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.4
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• pp.482-486
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• 2007

Basic objective of this research was to compare the milk yield and composition of New Zealand White transgenic rabbit females expressing recombinant human factor VIII (hFVIII) in mammary gland during lactation with that of non-transgenic rabbit females of the same age during 30 days of lactation. Transgenic founders were generated by the microinjection of foreign DNA (mWAP-hFVIII gene construct) into the egg. F1, F2 and F3 generations of transgenic rabbits were obtained after mating of transgenic founder rabbits with non-transgenic rabbits. The amount of milk rejected was measured by weight-suckle-weight method at $10^{th}$, $20^{th}$and $30^{th}$ day of lactation. Quality of milk (content of fat, protein, lactose, dry ash, and some minerals) from transgenic and non-transgenic rabbit was also determined. Comparison of milk yield, determined by weight-suckle-weight method, showed significantly higher (p<0.05) milk production at day 20 of first lactation in non-transgenic females, but on the same day of second lactation higher milk yield was measured in transgenic ones. Significantly higher (p<0.05) content of milk fat and protein was determined in transgenic milk whilst higher content of lactose was found in non-transgenic milk. The content of minerals (calcium, phosphorus, magnesium and sodium) did not differ in both experimental and control groups. Our results showed that milk yield and composition of transgenic rabbit females (mammary specific transgenic over-expression of hFVIII) over several generations is only slightly and transiently different from milk yield of non-transgenic females, which had no significant consequence on the litter size and viability.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.6
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• pp.857-864
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• 2006

The aim of this study was to apply the Cornell net carbohydrate and protein system (CNCPS) in subtropical Taiwan. This was done by means of 3 trials, viz, in situ, lactation and metabolic trials, the latter using the urinary purine derivatives (UPD) to estimate the ruminal microbial yield. Dietary treatments were formulated according to different nutrient requirement systems including, (1) a control NRC78 group on NRC (1978), (2) a NRC88 group on NRC (1988), and (3) a CNCPS group on Cornell Net carbohydrate and protein system model. Results from the lactation trial showed that DM intake (DMI) was higher (p<0.05) in the NRC78 than the other treatment groups. The treatments did not significantly influence milk yield, but milk yield after covariance adjustment for DMI was higher in the CNCPS group (p<0.05). The FCM, milk fat content and yield were greater in both the NRC78 and the NRC88 group over the CNCPS group (p<0.05). The treatments did not significantly influence the DMI adjusted FCM. The solid-non-fat and milk protein contents were higher in the CNCPS group (p<0.05) with or without DMI covariance adjustment. Lactating efficiency was higher in the CNCPS group (p<0.05) compared to the other groups. The significantly lowest milk urea-N (MUN) with better protein utilization efficiency in the CNCPS group (p<0.05) suggested that less N would be excreted into the environment. Cows in the CNCPS group excreted significantly more and the NRC88 group significantly less urinary purine derivatives (UPD) implying that more ruminal microbial protein was synthesized in the CNCPS over the NRC88 group. The CNCPS could become the most useful tool in predicting the trends in milk yield, microbial yield and MUN.

• Korean Journal of Food Science and Technology
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• v.30 no.3
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• pp.574-580
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• 1998

The technical feasibility of removing cholesterol from milk fat by supercritical carbon dioxide $(SC-CO_2)$ extraction followed by adsorption on different adsorbents and of fractionating milk fat into different fatty acid composition at $40^{\circ}C/276$ bar was investigated. Cholesterol could be selectively removed from milk fat by adsorption on a typical commercial florisil with $SC-CO_2$ extraction. Lower weight ratio of milk fat feed to florisil showed higher reduction of cholesterol, but gave lower yield in the milk fat fractions. The effective capacity of florisil for removing cholesterol from milk fat was 2.0g/g, which is the ratio of the fat feed to the adsorbent for 89% cholesterol reduction with a fat yield of 57.5%. Fatty acid composition showed higher short-chain and lower unsaturated long-chain fatty acids in the extracted fractions. Milk fat fractionation method by supercritical fluid extraction in coupled with adsorption would appear suitable for removing undesirable ingredients such as cholesterol and for enriching short-chain fatty acids in the fractions.