• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.650-657
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• 2002

Two experiments were conducted to evaluate the effects of forage:concentrate ratios and feeding principles on milk yield, milk composition, body weight change, postpartum oestrus and feed cost. A total of 36 crossbred F1 cows (Holstein Friesian${\times}$Local Yellow) in the 8th week of lactation were used. In each experiment, animals were divided into three groups using a randomized block design according to the milk yield of the previous eight weeks. Cows were fed 30, 50 and 70% concentrate in the diet based on DM. In experiment 1 (Fc), cows were given the same amount of DM with constant ratios of forage and concentrate within treatments. In experiment 2 (Fa), cows were given the same constant amounts of concentrate as in experiment 1 and ad libitum forage. The forage consisted of a natural grass mixture based on 5 species of grasses with high nutritive values. There was no difference in total DM intake between treatments within experiments. However, cows fed forage ad libitum had higher DM intakes compared to cows fed constant forage (1.6, 4.5 and 9.5% for cows fed 70, 50 and 30% forage, respectively). Daily milk yield of cows fed forage ad libitum was higher than that of cows fed constant forage:concentrate ratio. Within experiment, milk yield was highest for cows fed 30% DM forage, followed by cows fed 50% and then cows fed 70% forage (11.17, 10.98 and 10.71 for the 30Fc, 50Fc and 70Fc cows; 11.73, 11.16 and 10.81 kg for the 30Fa, 50Fa and 70Fa cows, respectively). Decreased forage ratio in the diets resulted in decreased milk fat content and tended to increase milk protein. Increased concentrate ratio in the diet and feeding forage ad libitum increased body weight gain. The effect of forage:concentrate ratio on postpartum oestrus was not significant. The feed cost per kg milk produced was lowest for the cows fed 70% forage. It is concluded that increased ratio of concentrate resulted in increased body weight gain, milk yield, milk protein, and decreased milk fat. Feeding forage ad libitum increased feed intake, milk yield and body weight gain. The ratio of 50% forage is more suitable for milk production and animal condition, but in terms of feed cost and under the conditions of small dairy farmers, the 70% ad libitum forage feeding is recommended.

• Journal of Animal Science and Technology
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• v.48 no.6
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• pp.839-846
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• 2006

This study was conducted to evaluate the effects of dietary addition of sucrose, propylene glycol and Tween 80 (Polysorbate 80 : Non-ionic Surfactants) on pre-partum (21 d) and post-partum (21 d) nutrients intake, blood metabolites, occurrence of metabolic disorders, milk yield and its composition in Holstein cows. Two basal diets were formulated each for pre- and post-partum period. The diets were mixed daily and fed at ad libitum to transitional cows. Forty cows of similar parity and milk yield were randomly divided into four groups (ten animals in each). The cows in three groups were supplemented either with 280g of sucrose/day (SU), SU+64g propylene glycol/day (SUP) or SUP+50g Tween80/day (SUPT). The feed for the fourth group was not supplemented and this group served as control (C). Pre-partum DM, total digestible nutrients (TDN), and crude protein (CP) intake was similar in cows fed C, SU, SUP, and SUPT diets. Post-partum DM, TDN, and CP intakes were the highest with SUPT diet followed by SU, SUP and control diets. Pre-partum blood non-esterified fatty acids (NEFA) concentration was noticed significantly higher in cows fed control diet compared to those fed SU, SUP and SUPT diets. The concentration of NEFA was similar at calving and during post-partum period across cows fed different experimental diets. Blood glucose and Ca concentration during pre- and post-partum periods were not significantly different in cows fed C, SU, SUP and SUPT diets. Milk yield (kg/day) was similar in cows fed different experimental diets. However, milk fat percent and 4% fat corrected milk yield were higher in cows fed SU diet (p<0.05) followed by SUP, SUPT and C diets. One case of ketosis was recorded in cows fed control diet however its occurrence was not observed in cows fed other diets. Occurrence of retained placenta and mastitis was numerically higher in cows fed control diet compared with those fed SU, SUP and SUPT diets. In conclusion, the NIS and propylene glycol feeding along with sucrose could improve the 4% fat corrected milk and fat yield in early lactating cows with significant reduction in NEFA and metabolic disorders during transitional period.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.7
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• pp.943-954
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• 2009

Diacylglycerol acyltransferase (DGAT) is a key enzyme that catalyzes the final and rate-limiting step of triglyceride synthesis. Both DGAT1 and DGAT2 genes code proteins with DGAT activity. Studies have shown DGAT1 polymorphisms associate with intramuscular fat deposition in beef cattle, but fewer associations between DGAT2 and beef cattle economic traits have been reported. The objective of this study was to investigate single nucleotide polymorphism (SNP) in intron3 of bovine DGAT2 and evaluate the associations of that with carcass, meat quality, and fat yield traits. Test animals were 157 commercial feedlot steers belonging to 3 Chinese native breeds (22 for Luxi, 24 for Jinnan, and 23 for Qinchuan), 3 cross populations (20 for Charolais${\times}$Fuzhou, 18 for Limousin ${\times}$Luxi, and 17 for Simmental${\times}$Jinan) and 1 Taurus pure breed population (16 Angus steers). In the current study, 15 SNP were discovered in intron3 and exon4 of DGAT2 at positions 65, 128, 178, 210, 241, 255, 270, 312, 328, 334, 365, 366, 371, 415, and 437 (named as their positions in PCR amplified fragments). Only 7 of them (128, 178, 241, 270, 312, 328, and 371) were analyzed, because SNP in three groups (65-128-255, 178-210-365 and 241-334-366) were in complete linkage disequilibrium within the group, and SNP 415 was a deletion and 437 was a null mutation. Frequencies for rare alleles in the 3 native breed populations were higher than in the 3 cross populations for 178 (p = 0.04), 270 (p = 0.001), 312 (p = 0.03) and 371 (p = 0.002). A general linear model was used to evaluate the associations between either SNP genotypes or allele substitutions and the measured traits. Results showed that SNP 270 had a significant association with the fat yield associated with kidney, pelvic cavity, heart, intestine, and stomach (KPHISY). Animals with genotype CC and CT for 270 had less (CC: -7.71${\pm}$3.3 kg and CT: -5.34${\pm}$2.5 kg) KPHISY than animals with genotype TT (p = 0.02). Allele C for 270 was associated with an increase of -4.26${\pm}$1.52 kg KPHISY (p = 0.006) and $-0.92{\pm}0.45%$ of retail cuts weight percentage (NMP, Retail cuts weight/slaughter body weight) (p = 0.045); allele G for 312 was associated with an increase of -5.45${\pm}$2.41 kg KPHISY (p = 0.026). An initial conclusion was that associations do exist between DGAT2 gene and carcass fat traits. Because of the small sample size of this study, it is proposed that further effort is required to validate these findings in larger populations.

• Animal Bioscience
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• v.37 no.6
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• pp.1110-1120
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• 2024

Objective: This study was conducted to evaluate the effect of offering (OSF) or not (NSF) supplemental feed at milking on temperament (TS), udder hygiene (UHS) and body condition (BCS) scores, and milk yield per milking (MYM), milk quality traits, and profitability of primiparous Anatolian buffalo cows at 90 days of lactation confined in closed-tied (CB) or semi-open free-stall (OB) barns. Methods: In Experiment I, 108 cows were selected to encompass four treatments (OBOSF, OB-NSF, CB-OSF, and CB-NSF) of 27 cows, considering barn type (OB and CB) and supplementary feed (OSF and NSF) at milking. In Experiment II, 60 OB cows were selected to encompass one of five groups of 12 cows each: i) no supplemental feed (CON), ii) commercial concentrate (CC), iii) CC + corn silage (CCS), iv) CCS + alfalfa hay (CSA), or v) CC + ryegrass silage (CRS) at milking. Results: The TS and UHS of the OB and OSF cows were lower (better) than those of the CB and NSF cows, respectively. The OSF increased milk protein, lactose, and solids-not-fat but decreased milk freezing point and electrical conductivity compared with the NSF. The MYM and milk fat of the OB-OSF cows were higher than those of the OB-NSF and CBNSF cows. The TS and UHS of the cows negatively correlated with MYM and some milk chemicals (fat, protein, and solids-not-fat), but BCS correlated positively. The TS and milk electrical conductivity of the CCS, CSA, and CRS cows were lower than those of the CON and CC cows, but BCS, MYM, and milk fat were higher. Partial budget analysis identified a higher net profit for supplemental feed-offered groups (OB-OSF, CCS, CSA, and CRS). Conclusion: Offering roughage with concentrates at milking for indoor primiparous buffalo cows is more conducive to well-being, milk yield, milk quality, and economy.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.901-903
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• 1999

Twenty-four multiparous crossbred Friesian dairy cows (60-90 days in lactation) were randomly assigned into a $2{\times}2$ factorial arrangement in a randomized complete block design. Factors were two levels of concentrate supplementation (1:2, high vs 1:1.2, very high; concentrate:milk yield) and two levels of high-quality feed block (HQFB) supplementation (non vs ad libitum block licking). Ruzi grass (Brachiaria ruziziensis) was fed as a roughage throughout the 70 day feeding trial. High level of concentrate fed group resulted in higher roughage and HQFB intakes, compared with very high concentrate supplemented group. HQFB supplementation tended to increase roughage intake and significantly improved milk yield (2 kg/hd/d in high concentrate supplementation) and quality (% fat) which resulted in higher economical return. HQFB was recommended to be used as a strategic supplement in lactating dairy cows especially when fed on low-quality roughages or crop residues.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.10
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• pp.1525-1538
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• 2007

Because of the increasing evidence of potential benefits of conjugated linoleic acid (CLA) on human health, there is a need to investigate its status in commercial dairies and develop feeding strategies to enhance the content and supply of CLA in milk and milk products. A two-year experiment was conducted to study the status of milk fat c-9, t-11 CLA on four selected commercial dairy farms in Utah (two) and Idaho (two), USA. Farms A and C grazed cows on pasture and supplemented with 7.0 kg/cow per day of their respective grain mixes during summer, while conserved forage and grain mix was fed during winter. Farm B fed a total mixed diet all year, with 10% of diet dry matter as fresh cut pasture during summer. Farm D had 1/3 of its cows grazed on pasture and supplemented with a total mixed diet during summer, while the rest were fed a total mixed diet. All cows in Farm D were fed a total mixed diet during winter. Farms A, B, C, and D had on average 80, 400, 150, and 500 milking cows, respectively, with Holstein or its crosses as the major breed. On a year-round basis, Farms A and C produced milk with 60% or more milk fat c-9, t-11 CLA and transvaccenic acid (TVA) contents than Farm B. Similarly, Farm D produced 30% or more c-9, t-11 CLA and TVA in milk than Farm B. Milk fat content of CLA and TVA was 150-200% more during summer compared with winter. Individual cows varied from 0.16 to 2.22% in milk fat c-9, t-11 CLA contents and 89% of the cows had c-9, t-11 CLA contents between 0.3 and 1.0% of milk fat. Individual cow variation was larger on Farms A and C compared with Farm D, with least variation on Farm B. Variation was larger in summer than in winter. The bulk tank milk c-9, t-11 CLA content varied from 0.27 to 1.35% of milk fat. Cows on Farms A and C produced similar or higher amounts of milk fat c-9, t-11 CLA on a daily basis even though their milk yield was lowest among the dairies. Concentration and supplies of c-9, t-11 CLA and TVA were highest from June through September and lowest from February through April, which should be the months for targeting improvement in the content and supply of milk fat c-9, t-11 CLA and TVA.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.1
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• pp.29-38
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• 2012

This study was carried out to compare the agronomic characteristics, forage yield, and feed value of nine sorghum ${\times}$ sudangrass hybrids at paddy field cultivation. The nine recommended sorghum ${\times}$ sudangrass hybrids used in this study were P877F, Cow pow, Turbo gold, Maxi graze, Jumbo, G7, Sweet home, Honey chew BMR and Green star. Plant height, leaf number, stem diameter, and stem hardness were higher in Jumbo than other varieties (P<0.01). Sugar content (brix) was higher in order of P877F > Sweet home > maxi graze > Green star > Cow pow > G7 (P<0.01). Fresh (86,199kg/ha) and dry matter yield (16,206 kg/ha) of Jumbo were higher than other varieties (P<0.01). Crude protein was the highest in Maxi graze (6.5%), but crude fat was the highest in Honey chew BMR as 2.1% (P<0.01). NDF and ADF of Jumbo and G7 were higher than other varieties (P<0.01). TDN was higher in order of Maxi graze > Sweet home > P877F > Honey chew BMR > Green star, but no significant differences were found among the varieties. Minerals were the highest in Cow pow (15,020.5 mg/kg), and Sweet home (6,222.6 mg/kg) was the lowest as compared to other varieties (P<0.01). Total amino acids were higher in order of Maxi graze > Sweet home > Turbo gold > Honey chew BMR > Jumbo (P<0.01). Crude protein yield and crude fat yield were the highest in Turbo gold (814.6 kg/ha) and Honey chew BMR (309.8kg/ha), respectively (P<0.01). Mineral yield was the highest in Cow pow as 207.6 kg/ha (P<0.01). Amino acid yield (592.2 kg/ha) and TDN yield (10,194 kg/ha) were the highest in Sweet home. Based on the above results, sorghum ${\times}$ sudangrass hybrid varieties should be selected on the basis of the purpose of use. Because, each varieties showed various growth characteristics and nutrition yield (protein, fat, mineral, amino acid, TDN).

• Food Science of Animal Resources
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• v.40 no.3
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• pp.426-443
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• 2020

In order to investigate the use of oil in water gelled emulsion (GE) prepared with healthier oil combinations as beef fat replacer in the fresh chicken sausage formulations, four batches of fresh sausages were produced. The first batch was control (C) sample formulated with %100 beef fat, other batches were codded as GE50, GE75, and GE100 respective to the percentage of beef fat replaced with GE. The addition of GE to sausage formulation resulted in an increment in moisture and protein contents while a decrement was observed in fat content (p<0.05). pH, cooking yield and water holding capacity values of GE added samples were found lower than C (p<0.05). GE addition caused lower CIE L* values in samples, however, this trend was not observed in CIE a* and CIE b* values. Initially, the lowest peroxide and the highest TBARS values were recorded in GE100 samples on the 0^th d (p<0.05). Peroxide and TBARS values were in the limits. The texture of samples was softened while total saturated fatty acid content reduced up to 52.61% with the incorporation of GE (p<0.05). Taken together, our results showed that GEs can be used as fat replacers in meat product formulations without causing undesirable quality changes.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.3
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• pp.155-162
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• 2014

This study was conducted to investigate the influence of the mixed application of chemical fertilizer (CF) and liquid swine manure (LSM) on the agronomic characteristics, dry matter yield, amino acids, minerals, and free sugars in cultivating Sorghum ${\times}$ Sudangrass hybrid (SSH) on paddy soil. The field experiment was designed in a randomized block design with three replications and consisted of CF 100% (C), CF 70% + LSM 30% (T1), CF 50% + LSM 50% (T2), CF 30% + LSM 70% (T3), and LSM 100% treatment (T4). The application of LSM was based solely on the nitrogen. Plant length, leaf length, leaf width and number of leaf were significantly higher in T4 (p<0.05), but stem diameter did not show significant differences among treatments. Stem hardness increased significantly (p<0.05) as the LSM application rate decreased, but sugar degree decreased significantly (p<0.05) as the LSM application rate decreased. Fresh yield, dry matter yield and TDN yield were the highest in T4, whereas the lowest in T2 (p<0.05). Crude protein, crude fat and crude ash were the highest in C, T4 and T2, respectively (p<0.05). However, NDF and ADF did not show significant difference among treatments. Crude fiber decreased significantly (p<0.05) as the LSM application rate increased. The total mineral content was decreased significantly (p<0.05) as the LSM application rate increased. Total amino acid content was higher in the order of T1> C> T3> T4> T2 (p<0.05). Free sugar content increased significantly (p<0.05) as the LSM application rate increased. The analysis of all the above results suggests that the application of liquid swine manure is very effective, considering the yield performance and the content of sugar degree and free sugar. In addition, liquid swine manure may be possible to grow Sorghum ${\times}$ Sudangrass hybrid without chemical fertilizer.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.2
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• pp.59-66
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• 2001

In order to determine the influence of nitrogen on agronomic characters, forage yield and quality, a Kenaf was cultured on the volcanic ash soil at the Experimental Farm of Cheju national University under the seven levels of nitrogen rates(0, 50, 100, 150, 200, 250kg/ha) from April 25 to Dec. 25, 1999. The plant height increased by increase of nitrogen rate, showing longest 250kg/ha with 286.6cm and shortest at no application plot with 255.7cm. The difference on leaf number, leaf withering number, stem diameter and branches number by nitrogen rate showed a similar tendency to the plant height. Increasing N rate from 0 to 250kg/ha fresh forage yield form 55.8 to 99.8MT/ha, dry matter (DM) yield from 8.8 to 15.8MT/ha, crude protein(CP) yield form 1.2 to 3.1MT/ha, total digestible nutrients (TDN) yield from 3.8 to 8.6MT/ha. However, no significant differences in these yields were found between 200 and 250kg N/ha. Nitrogen uptake increased form 192.9 to 496.2 kg/ha but N use efficiency decreased form 95.0 to 66.6 kg DM$^{a}$ /kg N with increasing from 0 to 250 kg/ha. As N rate increased from 0 to 250kg/ha, leaf and stem out of CP, crude fat (CF), nitrogen free extract (NFE), TDN contents increased from 20.1% to 25.8% and from 9.7% to 12.4%, from 5.6% to 8.1% and from 3.3% to 4.4%, from 36.1% to 40.2% and from 21.9% to 32.4%, from 59.3% to 75.0% and from 32.2% to 38.2%, respectively, while leaf and stem out of crude fiber decreased from 18.5% to 16.7% and from 51.5% to 39.3%. Based on the these findings, the optimum N rate for forage production of kenaf seems to be about 200 kg/ha in atmospheric phenomena and volcanic ash soils of jeju island.