• Journal of Advanced Marine Engineering and Technology
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• v.23 no.2
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• pp.252-258
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• 1999

As an effective means to convey crushed materials from seabed to on board ship and to raise hazardous or abrasive liquids air-lift pump provides a reliable mechanism due to its simple config-uration and easy-to-operate principle. The present study is focused on fundamental investigation of related performance by the analysis program based on the gas-liquid two-phase flow in circular pipes. The program covers pump operating in isothermal and vertical two-phase flow with Newto-nian liquids. it is summarized as important result that an optimum air mass flow rate exists for the maximum lifted liquid mass flow rate in terms of a given submergence rates and furthermore attachment of downcomer gives little effects on riser performance the conveyed liquid flow rate increases with larger submergence rate.

• Fisheries and Aquatic Sciences
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• v.15 no.2
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• pp.107-115
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• 2012

In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of $57.9^{\circ}C$ and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.