• 한국방재학회 논문집
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• 제8권3호
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• pp.37-42
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• 2008

본 연구는 지하철에서 화재발생시 최적의 제연모드 예측을 목적으로 수치해석을 수행하였다. 현재 국내 지하철 화재발생시 제연모드는 승강장 내 화재구역 배기, 비화재구역 급기 또는 정지로 설정 되어있다. 수치해석 조건은 2가지 국내 비상시 제연모드 및 발화 4분(승강장 피난허용시간, NFPA 130) 이전까지 승강장에서 배기를 하며, 발화 4분이 후 터널 배기로 전환되는 스위치모드의 3가지 경우에 대하여 비교 분석하였다. 승객 호흡높이(1.7 m)를 기준으로 열, 일산화탄소 및 가시거리를 비교하였다. 수치해석 결과 급기팬 작동은 연기를 교란시키고 확산시킬 수 있으며, 또한 승강장 및 터널 제연모드로 작동할 경우 승강장의 제연모드로만 작동한 경우에 비하여 연기 배출에 효과적임을 확인할 수 있었다.

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2008년도 정기학술발표대회 논문집
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• pp.877-882
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• 2008

오피스 지하주차장의 기둥간격은 기준층의 업무환경과 효율적인 주차구획을 고려하여 계획된다. 그러나 비정형의 지하주차장에 정형의 기둥간격에 의한 주차계획을 하다보면 주차장내 많은 부분에 유휴공간이 형성된다. 대부분 주차장의 양 모서리 부분은 주차장 환기시설로서 팬룸이 배치되고, 모서리 일부분은 주차램프가 위치하게 되며 잔여 공간 및 유휴공간에는 창고를 배치할 수 있다. 이때 지하주차장 창고는 필요로 하는 오피스 임차인들에게 외부의 전문창고 임대업체보다 낮은 가격으로 임대하게 된다. 이러한 경우 창고를 만들기 위한 초기 공사비와 유지비용 등이 소요된다. 본 연구에서는 이러한 총 LCC와 초기 투자로 벌어들일 수 있는 임대수익 간의 경제성 분석을 통해 사업 초기 지하주차장 유휴공간에 창고를 설치하는 것에 대한 경제성을 분석하였다. 분석결과, 현재 사용되고 있는 창고임대료를 기준으로 매년 임대수익을 산정하였을 경우, 2년째부터 수익이 보장되어 경제성이 있는 것으로 판단되었다.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.1933-1942
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• 2016

A novel ${\alpha}-glucoside$ hydrolase (named PspAG97A) from glycoside hydrolase family 97 (GH97) was cloned from the deep-sea bacterium Pseudoalteromonas sp. K8, which was screened from the sediment of Kongsfjorden. Sequence analysis showed that PspAG97A belonged to GH97, and shared 41% sequence identity with the characterized ${\alpha}-glucoside$ BtGH97a. PspAG97A possessed three key catalytically related glutamate residues. Mutation of the glutamate residues indicated that PspAG97A belonged to the inverting subfamily of GH97. PspAG97A showed significant reversibility against changes in salt concentration. It exhibited halophilic ability and improved thermostability in NaCl solution, with maximal activity at 1.0 M NaCl/KCl, and retained more than 80% activity at NaCl concentrations ranging from 0.8 to 2.0 M for over 50 h. Furthermore, PspAG97A hydrolyzed not only ${\alpha}-1,4-glucosidic$ linkage, but also ${\alpha}-1,6-$ and ${\alpha}-1,2-glucosidic$ linkages. Interestingly, PspAG97A possessed high catalytic efficiency for long-chain substrates with ${\alpha}-1,6-linkage$. These characteristics are clearly different from other known ${\alpha}-glucoside$ hydrolases in GH97, implying that PspAG97A is a unique ${\alpha}-glucoside$ hydrolase of GH97.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2010년도 제35회 추계학술대회논문집
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• pp.101-104
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• 2010

최근 무인기 혹은 소형 항공기들에 대한 수요가 증가함에 따라 소형 터보제트 혹은 터보팬 엔진의 수요가 꾸준히 증가하고 있다. 이러한 소형 엔진은 요구 추력을 달성하면서 크기와 무게를 줄이는 것이 가장 중요한 설계 인자인데, 이는 곧 비추력(Thrust to Weight) 값이 매우 높음을 의미한다. 비추력 값이 높은 엔진을 설계하기 위해서, 압축기 혹은 터빈 등의 핵심구성품의 경우 크기를 줄이고 단수를 줄이는 방법이 가장 효과적이다. 특히 축류압축기의 경우 일반적으로 다단으로 구성하는데 여러 단으로 구성된 압축기 중에서 단수를 줄이고 남은 단에서의 압력비를 높이는 '고부하 압축기'를 채용함으로써 이러한 문제를 해결할 수 있다. 최근 국내에서도 축류형 압축기에 대한 개발의 필요성이 꾸준히 제기되고 있으며, 이에 본 논문에서는 고부하 축류형 압축기의 설계 및 성능 리뷰 중 주요한 사항에 대해 소개하고자 한다.

• Advances in aircraft and spacecraft science
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• 제3권3호
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• pp.243-257
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• 2016

The increase of air traffic volume has brought an increasing amount of issues related to carbon and NOx emissions and noise pollution. Aircraft manufacturers are concentrating their efforts to develop technologies to increase aircraft efficiency and consequently to reduce pollutant discharge and noise emission. Ultra High By-Pass Ratio engine concepts provide reduction of fuel consumption and noise emission thanks to a decrease of the jet velocity exhausting from the engine nozzles. In order to keep same thrust, mass flow and therefore section of fan/nacelle diameter should be increased to compensate velocity reduction. Such feature will lead to close-coupled architectures for engine installation under the wing. A strong jet-wing interaction resulting in a change of turbulent mixing in the aeroacoustic field as well as noise enhancement due to reflection phenomena are therefore expected. On the other hand, pressure fluctuations on the wing as well as on the fuselage represent the forcing loads, which stress panels causing vibrations. Some of these vibrations are re-emitted in the aeroacoustic field as vibration noise, some of them are transmitted in the cockpit as interior noise. In the present work, the interaction between a jet and wing or fuselage is reproduced by a flat surface tangential to an incompressible jet at different radial distances from the nozzle axis. The change in the aerodynamic field due to the presence of the rigid plate was studied by hot wire anemometric measurements, which provided a characterization of mean and fluctuating velocity fields in the jet plume. Pressure fluctuations acting on the flat plate were studied by cavity-mounted microphones which provided point-wise measurements in stream-wise and spanwise directions. Statistical description of velocity and wall pressure fields are determined in terms of Fourier-domain quantities. Scaling laws for pressure auto-spectra and coherence functions are also presented.

• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.8-15
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• 2021

More and more available fungal genome sequence data reveal a large amount of secondary metabolite (SM) biosynthetic 'dark matter' to be discovered. Heterogeneous expression is one of the most effective approaches to exploit these novel natural products, but it is limited by having to clone entire biosynthetic gene clusters (BGCs) without errors. So far, few effective technologies have been developed to manipulate the specific large DNA fragments in filamentous fungi. Here, we developed a fungal BGC-capturing system based on CRISPR/Cas9 cleavage in vitro. In our system, Cas9 protein was purified and CRISPR guide sequences in combination with in vivo yeast assembly were rationally designed. Using targeted cleavages of plasmid DNAs with linear (8.5 kb) or circular (8.5 kb and 28 kb) states, we were able to cleave the plasmids precisely, demonstrating the high efficiency of this system. Furthermore, we successfully captured the entire Nrc gene cluster from the genomic DNA of Neosartorya fischeri. Our results provide an easy and efficient approach to manipulate fungal genomic DNA based on the in vitro application of Cas9 endonuclease. Our methodology will lay a foundation for capturing entire groups of BGCs in filamentous fungi and accelerate fungal SMs mining.

• The Korean Journal of Physiology and Pharmacology
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• 제25권2호
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• pp.97-109
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• 2021

Neonatal hypoxia/ischemia (H/I), injures white matter, results in neuronal loss, disturbs myelin formation, and neural network development. Neuroinflammation and oxidative stress have been reported in neonatal hypoxic brain injuries. We investigated whether Paeoniflorin treatment reduced H/I-induced inflammation and oxidative stress and improved white matter integrity in a neonatal rodent model. Seven-day old Sprague-Dawley pups were exposed to H/I. Paeoniflorin (6.25, 12.5, or 25 mg/kg body weight) was administered every day via oral gavage from postpartum day 3 (P3) to P14, and an hour before induction of H/I. Pups were sacrificed 24 h (P8) and 72 h (P10) following H/I. Paeoniflorin reduced the apoptosis of neurons and attenuated cerebral infarct volume. Elevated expression of cleaved caspase-3 and Bad were regulated. Paeoniflorin decreased oxidative stress by lowering levels of malondialdehyde and reactive oxygen species generation and while, and it enhanced glutathione content. Microglial activation and the TLR4/NF-κB signaling were significantly down-regulated. The degree of inflammatory mediators (interleukin 1β and tumor necrosis factor-α) were reduced. Paeoniflorin markedly prevented white matter injury via improving expression of myelin binding protein and increasing O1-positive olidgodendrocyte and O4-positive oligodendrocyte counts. The present investigation demonstrates the potent protective efficiency of paeoniflorin supplementation against H/I-induced brain injury by effectually preventing neuronal loss, microglial activation, and white matter injury via reducing oxidative stress and inflammatory pathways.

• 한국재료학회지
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• 제31권12호
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• pp.657-664
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• 2021

To build a highly active photocatalytic system with high efficiency and low cast of TiO₂, we report a facile hydrothermal technique to synthesize Ag₂Se-nanoparticle-modified TiO₂ composites. The physical characteristics of these samples are analyzed by X-ray diffraction, scanning electron microscopy with energy dispersive X-ray analysis, transmission electron microscopy and BET analysis. The XRD and TEM results show us that TiO₂ is coupled with small sized Ag₂Se nanoplate, which has an average grain size of about 30 nm in diameter. The agglomeration of Ag₂Se nanoparticles is improved by the hydrothermal process, with dispersion improvement of the Ag₂Se@TiO₂ nanocomposite. Texbrite BA-L is selected as a simulated dye to study the photodegradation behavior of as-prepared samples under visible light radiation. A significant enhancement of about two times the photodegradation rate is observed for the Ag₂Se@TiO₂ nanocomposite compared with the control sample P25 and as-prepared TiO₂. Long-term stability of Ag₂Se@TiO₂ is observed via ten iterations of recycling experiments under visible light irradiation.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.369-375
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• 2004

Gas turbine engine simulation in terms of transient, steady state performance and operational characteristics is complex work at the various engineering functions of aero engine manufacturers. Especially, efficiency of control system design and development in terms of cost, development period and technical relevance implies controlling diverse simulation and identification activities. The previous engine simulation has been accomplished within a limited analysis area such as fan, compressor, combustor, turbine, controller, etc. and this has resulted in improper engine performance and control characteristics because of limited interaction between analysis areas. In this paper, we propose a new simulation methodology for gas turbine engine performance analysis as well as its digital controller to solve difficulties as mentioned above. The novel method has particularities of (ⅰ) resulting in the integrated control simulation using almost every component/module analysis, (ⅱ) providing automated math model generation process of engine itself, various engine subsystems and control compensators/regulators, (ⅲ) presenting total sophisticated output results and easy understandable graphic display for a final user. We call this simulation system GT3GS (Gas Turbine 3D Graphic Simulator). GT3GS was built on both software and hardware technology for total simulation capable of high calculation flexibility as well as interface with real engine controller. All components in the simulator were implemented using COTS (Commercial Off the Shelf) modules. In addition, described here includes GT3GS main features and future works for better gas turbine engine simulation.

• Structural Engineering and Mechanics
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• 제81권2호
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• pp.179-194
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• 2022

The bending, buckling and free vibration responses of functionally graded material (FGM) beams are investigated semi-analytically by the scaled boundary finite element method (SBFEM) in this paper. In the concepts of the SBFEM, the dimension of computational domain can be reduced by one, therefore only the axial dimension of the beam is discretized using the higher order spectral element, which reduces the amount of calculation and greatly improves the calculation efficiency. The governing equation of FGM beams is derived in detail by the means of the principle of virtual work. Compared with the higher-order beam theory, fewer parameters and simpler control equations are used. And the governing equation is transformed into a first-order ordinary differential equation by introducing intermediate variables. Analytical solutions of the governing equation can be obtained by pade series expansion in the direction of thickness. Numerical example are compared with the numerical solutions provided by the previous researchers to verify the accuracy and applicability of the proposed method. The results show that the proposed formulations can quickly converge to the reference solutions by increasing the order of higher order spectral elements, and high accuracy can be achieved by using a small number of the elements. In addition, the influence of the structural sizes, material properties and boundary conditions on the mechanical behaviors of FG beams subjected to different load types is discussed.