• Communications of the Korean Mathematical Society
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• v.31 no.2
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• pp.395-414
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• 2016

In this work, we study on subdivision schemes reproducing polynomials and build a symmetric subdivision scheme reproducing polynomials of a certain predetermined degree, which is a slight variant of the family of Deslauries-Dubic interpolatory ones. Related to polynomial reproduction, a necessary and sufficient condition for a subdivision scheme to reproduce polynomials of degree L was recently established under the assumption of non-singularity of subdivision schemes. In case of stepwise polynomial reproduction, we give a characterization for a subdivision scheme to reproduce stepwise all polynomials of degree ${\leq}L$ without the assumption of non-singularity. This characterization shows that we can investigate the polynomial reproduction property only by checking the odd and even masks of the subdivision scheme. The minimal-support condition being relaxed, we present explicitly a general formula for the mask of (2n + 4)-point symmetric subdivision scheme with two parameters that reproduces all polynomials of degree ${\leq}2n+1$. The uniqueness of such a symmetric subdivision scheme is proved, provided the two parameters are given arbitrarily. By varying the values of the parameters, this scheme is shown to become various other well known subdivision schemes, ranging from interpolatory to approximating.

• Journal of Microbiology and Biotechnology
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• v.13 no.5
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• pp.706-709
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• 2003

Ginsenosides and polysaccharides were isolated from Panax ginseng C.A. Meyer (Family Araliaceae) by treating at low ($60^{\circ}C$, LT), mild ($100^{\circ}C$, MT), and high ($120^{\circ}C$, HT) temperatures, and their inhibitory effects on growth, infection, and VacA vacuolation of Helicobacter pylori (HP) were investigated. The molecular weights of polysaccharides decreased as the processing temperature increased. Ginseng polysaccharides inhibited the HP infection into KATO III cells, but did not inhibit growth of HP and VacA vacuolation of HeLa cells. HT polysaccharides showed the most potent inhibition with $IC_50$ value of 6.8 mg/ml. Ginseng saponins did not inhibit the infection of HP into KATO cells. However, 20(s)-protopanaxadiol showed the most potent inhibition of HP growth and vacuolation of HeLa by VacA toxin with $IC_50$ values of 0.05 and 0.067 mg/ml, respectively.

• Journal of Microbiology and Biotechnology
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• v.24 no.12
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• pp.1699-1706
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• 2014

A lichenase gene (mt-lic) was identified for the first time through function-based screening of a soil metagenomic library. Its deduced amino acid sequence exhibited a high degree of homology with endo-${\beta}$-1,3-1,4-glucanase (having both lichenase and chitosanase activities), encoded by the bgc gene of Bacillus circulans WL-12. The recombinant lichenase overexpressed and purified from Escherichia coli was able to efficiently hydrolyze both barley ${\beta}$-glucan and lichenan. The enzyme showed maximal activity at a pH of 6.0 at $50^{\circ}C$, with Azo-barley-glucan as the substrate. The metal ions $Mn^{2+}$, $Mg^{2+}$, $Ca^{2+}$, and $Fe^{2+}$ enhanced the enzymatic activity, whereas the $Cu^{2+}$ and $Zn^{2+}$ ions inhibited the enzymatic activity. The $K_m$ and $V_{max}$ values of the purified lichenase were determined to be 0.45 mg/ml and 24.83 U/min/mg of protein, respectively.

• Proceedings of the SCSK Conference
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• 2003.09a
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• pp.590-600
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• 2003

The matrix metalloproteinases (MMPs) are a family of enzymes responsible for degrading connective tissue. MMPs catalyze the breakdown of collagen from the extracellular matrix, leading to wrinkle formation and accelerated skin aging. Furthermore, ultraviolet irradiation causes increased expression of certain MMPs. In the extracellular matrix turnover, MMPs are interacting with endogenous regulators named tissue inhibitors of metalloproteinases (TIMPs). Using peptide substrate assays, it has been demonstrated that TIMP-MMP complexes interact highly specifically with $K_{i}$ values of 10$^{-9}$ -10$^{-16}$ M. Therefore applications for TIMP as inhibitor of collagen degradation are suggested for cosmetic anti-aging products to prevent wrinkle formation and loss of elasticity. To date four TIMP proteins (TIMP-1, TIMP-2, TIMP-3 and TIMP-4) have been identified which show a high degree in sequence similarity. The production of human TIMP-2, a 194-residue nonglycosylated protein, was performed by fed-batch culture of Escherichia coli. TIMP-2 accumulated in the bacterial cells in an insoluble form as inclusion bodies. The inclusion bodies were solubilized and the protein refolded to yield the native TIMP-2 in the active form. The integrity of the protein was confirmed by mass analysis, Edman sequencing and gel shift experiments with authentic samples. The inhibitory activity of the refolded and purified TIMP-2 was demonstrated with MMP-1 and MMP-2 assays using synthetic fluorogenic peptide substrates.s.

• Journal of the Korean Wood Science and Technology
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• v.22 no.3
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• pp.39-44
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• 1994

This study was carried out to investigate features pertaining to spiral thickenings, which was considered one of the most important diagnostic values, for wood identification. Species, kind of cells with spiral thickening, and ridge numbers of spiral thickening per axial mm were recorded in 71 families, 144 genera, 316 species of Korean hardwoods. Spiral thickening was observed in 128 of 316 species, about 40.5 % of all the investigated, and classified into 6 types on the basis of distributional patterns and morphological features as follows: 1. Type 1, present throughout all vessel element, which was found in 14 families, 19 genera, 43 species. 2. Type 2, present only in small vessel element, which was found in 18 families, 29 genera, 41 species. 3. Type 3, present both in small vessel element and wood fibers, which was found in 8 families, 17 genera, 29 species. 4. Type 4, present in wood fibers, which was found in 1 family, 1 genus, 1 species. 5. Type 5, present only in tail of vessel element, which was found in 4 families, 5 genera, 9 species. 6. Type 6, being present in vessel element faintly or partially, which was found in 2 families, 3 genera, 5 species.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.1
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• pp.81-85
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• 2013

Background: Early diagnosis of carcinoma of bladder remains a challenge. Survivin, a member of the inhibitor of apoptosis (IAP) protein family, is frequently activated in bladder carcinoma. The objective of this study was to investigate urinary survivin as a marker for diagnosis of urinary bladder. Materials and Methods: We examined urinary survivin concentration in 28 healthy individuals, 46 positive controls and 117 cases of histologically proven TCC prior to transurethral resection, using ELISA, and compared values with findings for urinary cytology. Results: Survivin was found to be significantly higher in the cancer group (P<0.05). A cut off value of 17.7 pg/ml was proposed, with an approximate sensitivity of 82.9% and specificity of 81.1% (P<0.0001), whereas urine cytology had a sensitivity of 66.7% and a specificity of 96.0%. Conclusions: Urinary survivin can be used as a non-invasive diagnostic biomarker for TCC bladder, both for primary and recurrent disease.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.8
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• pp.3953-3958
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• 2016

Prostatic cancer is the second cause of cancer-related death among men worldwide. The human papilloma viruses (HPVs) are a family of sexually transmitted viruses which have may have roles in the ethiology of inflammation in prostate leading to benign prostatic hyperplasia (BPH) and prostate cancer (PCa). In this study, we evaluated the frequency of different HPV types in prostatic cancer and benign prostatic hyperplasia (BPH) in Kerman province, southeast of Iran, using real-time PCR techniques. The aim of the present research was to clarify any association with prostatic carcinogenesis. Real Time PCR showed that HPV DNA was found in 20% of 200 PCa samples, 80 percent of these with high-risk HPV types, 40% with type-16,18, 30 % type-31,33 and 10% type 54. High risk HPV DNA was detected in only 2% of BPH samples. Values for low risk types were much higher. Our study provided a support for the role of high risk HPV infection in prostatic disease in Iranian patients, and association between presence of HPV DNA and prostate carcinoma. In particular, HPV 16 and18 might have an important role in prostate cancer.

• Advances in Traditional Medicine
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• v.7 no.2
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• pp.157-161
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• 2007

We undertook the present study to evaluate different pharmacological as antioxidant, antibacterial and cytotoxic activities of the crude ethanolic extract of the stem bark of Holarhena antidysenterica (Family: Apocynaceae). The antioxidant property of the extract was assessed by 1,1-diphenyl-2-picryl hydrazyl free radical scavenging assay. The extract showed antioxidant activity ($IC_{50}$ about ${\sim}08$ ${\mu}g/ml$), which was comparable to standard drug ascorbic acid ($IC_{50}$ about ${\sim}10$ ${\mu}g/ml$). The extract showed a broad spectrum of antibacterial activity against all tested gram positive and gram negative bacteria most prominent against Enterococci, Staphylococcus pyogenase and Shigella sonnie. And the zones of inhibitions were ranging from 10 - 21 mm for all the tested bacteria. Its cytotoxic property was evaluated by brine shrimp lethality bioassay. The extract showed significant lethality and the $LC_{50}$, $LC_{90}$ values were 80 ${\mu}g/ml$, 320 ${\mu}g/ml$ respectively. The results tend to suggest that the extract might possess chemical constituent(s) that are responsible for antioxidant, antibacterial and cytotoxic activities.

• Journal of the Korean housing association
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• v.13 no.1
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• pp.1-9
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• 2002

The purpose of this study was to analyze the differences housing needs between Korean and Japanese employed wives. Subjects consisted of 90 Korean employed and 141 Japanese employed wives who have children aged between 0-12 years old. The subjects were surveyed with a questionnaire specifically developed for this study to evaluate the housing needs of this growing segment of population. The data were analyzed using the SPSS statistical package. The results of descriptive statistics, x$^2$-test, multiple linear regressions are presented. The findings were as follows: 1) Korean employed wives depend on grandparents and relatives in their child-care and housework when the wives were out. On the contrary, Japanese employed wives put much more values on aid of their husband and community facilities fur child-care and housework. 2) The housing community needs showed significant differences. Korean employed wives wanted to provide a better physical environment for child-care and education of children more than Japanese employed wives. But Japanese employed wives needed more convenient community facilities that could be socialized housework than Korean employed wives. 3) Korean employed wives wanted gathered apartment house, and Japanese employed wives wanted row house. In comparison of working space needs in individual house, Korean employed wives wanted dining kitchen filled with many kinds of electric appliance including laundry space more, Japanese employed wives wanted LDK type which they have family interaction more and to depart laundry space from kitchen.

• Journal of the Korean Home Economics Association
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• v.27 no.2
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• pp.53-63
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• 1989

A untrition survey of early childhood was undertaken among 102 rural young children, aged 3 to 6, attending three day care centers of Hongcheon-gun, Kangwon province, in July of 1987, to investigate dietary and nutritional status. The results were obtained as follows: Mean values of height and arm circumference ranged from 96 to 97% of the KSRI's standards. However, mean value of weight met 91% of that standard. Mean value of hematocrit was 37.3±3.5%. According to the criterion established by the WHO, 3% of the subjects were proven to be anemic. Mean value of urinary urea nitrogen/creatinine ratio was 13.8±7.6; the higher the age lower the ratio was shown. Intake of energy and nutrients ranged from 63 to 88% of the RDAs. Carbohydrate provided 68% of total energy intake; protein accounted for 14%; fat provided 18%. Energy intake was divided among breakfast, lunch supper and snacks in a percentage ratio of 21:35:26:18. The survey clarified that the day care feeding largely supplemented the inadequate dietary intakes of these young children at home. Family factors, anthropometric measurements, biochemical results were positively correlated with nutrient intakes. Authors with this study can be contributed, as a reference, to develop the community nutrition programs as well as improving the quality of day care feeding.