• 한국어병학회지
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• 제37권1호
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• pp.147-153
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• 2024

The World Organization for Animal Health (WOAH) recommends two protocols (ITS and COI) for conventional PCR of G. salaris diagnosis. However, ITS PCR protocol may yield false-positive results, leading to unnecessary countermeasures. It's difficult to distinguish between G. salaris and false-positive by similar amplicon size of PCR, since the amplicon size of ITS PCR in G. salaris and false-positive was 1,300 and 1,187 bp, respectively. The nucleotide sequences of ITS false-positive in rainbow trout is 99.7% identical to previously reported host genome sequences of rainbow trout (Oncorhynchus mykiss) and 95.3 to 89.1% identical to those of other salmonid fish species. To reduce false-positive PCR band, PCR was performed by the different annealing temperature, but PCR bands were still detected. In RFLP analysis by HaeIII, the PCR product of G. salaris was digested into four bands of 512, 399, 234 and 154 bp, while the false-positive was digested into seven bands of 297, 263, 242, 144, 93, 80 and 68 bp. In the RFLP patterns digested by HindIII, G. salaris showed two bands of 659 and 640 bp, while false-positive had one fragment of 1,187 bp without any digestion. Therefore, the RFLP method of ITS PCR with HaeIII and HindIII can be used for differentiation between G. salaris and false-positive. These results might provide important information on the improvement of PCR diagnostic method of G. salaris.

• BMB Reports
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• 제35권2호
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• pp.248-250
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• 2002

Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, which was not generated during the reverse transcriptase step. The possibility exists that Taq DNA polymerase acts as a reverse transcriptase, generating cDNA from RNA during the PCR step. In order to test this hypothesis, we incubated samples with a DNAse-free RNAse after the cDNA synthesis. Comparison of the results that were obtained from these samples (incubated with or without DNAse-free RNAse) confirms that the reverse transcriptase activity of Taq DNA polymerase I is a possible source of false positive results when performing RT-PCR from intronless genes. Moreover, we describe here a simple and rapid method to overcome the false positive results that originate by this activity of Taq polymerase.

• 대한임상독성학회지
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• 제19권1호
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• pp.24-30
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• 2021

Purpose: In Korea, it is predicted that the proportion of drug abusers among patients visiting the emergency room will soon increase. Several emergency medical institutions in Korea are conducting field urine screening tests for poisoning. In this study, we investigated the characteristics and usefulness of urine toxicology screening tests. Methods: The medical records of patients with positive results for tetrahydrocannabinol and methamphetamine from urine toxicology screening tests at a tertiary university hospital from August 2016 to August 2019 were reviewed retrospectively. The subjects were classified into positive and false-positive groups, and their clinical characteristics were compared and analyzed. Results: Of the 2,026 patients surveyed, 823 patients (40.6%) tested positive for one or more drugs. Among them, 12 cases (0.6%) were positive for methamphetamine and 40 cases (2.0%) were positive for tetrahydrocannabinol. The positive and the false-positive rates for methamphetamine were 66.7% and 33.3%, respectively. The positive and the false-positive rates for tetrahydrocannabinol were 2.5% and 97.5%, respectively. Conclusion: Methamphetamine showed a relatively low false-positive rate in our study. Therefore, this test seemed to assist in diagnosing methamphetamine poisoning when considered together with the present illness and physical examination results. On the other hand, the high false-positive rate for tetrahydrocannabinol tests indicates that this test was unlikely to assist in diagnosing tetrahydrocannabinol poisoning. However, considering the growing trend of illegal drug abusers in Korea, it may still be useful as a diagnostic tool for identifying drug users.

• IEIE Transactions on Smart Processing and Computing
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• 제4권3호
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• pp.163-168
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• 2015

Many IP address lookup approaches employ Bloom filters to obtain a high-speed search performance. Especially, it has been recently studied that the search performance of trie-based algorithms can be significantly improved by adding Bloom filters. In such algorithms, the number of trie accesses can be greatly reduced because Bloom filters can determine whether a node exists in a trie without actually accessing the trie. Bloom filters do not have false negatives but have false positives. False positives can lead to unnecessary trie accesses. The false positive rate must thus be reduced to enhance the performance of lookup algorithms applying Bloom filters. One important characteristic of trie-based algorithms is that all the ancestors of a node are also stored. The proposed algorithm utilizes this characteristic in reducing the false positive rate of a Bloom filter without increasing the size of the memory for the Bloom filter. When a Bloom filter produces a positive result for a node of a trie, we propose to check whether the ancestors of the node are also positives. Because Bloom filters have no false negatives, the negatives of any of the ancestors mean that the positive of the node is false. In other words, we propose to use more Bloom filter queries to reduce the false positive rate of a Bloom filter in trie-based algorithms. Simulation results show that querying one ancestor of a node can reduce the false positive rate by up to 67% with exactly the same architecture and the same memory requirement. The proposed approach can be applied to other trie-based algorithms employing Bloom filters.

• 대한유전성대사질환학회지
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• 제16권1호
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• pp.18-23
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• 2016

TMS가 확산되고 검사가 많아짐에 따라 위양성 건수도 증가하고 있다. 본 연구에서는 2012년 1월 1일부터 2014년 12월 31일까지 단일 기관에서 출생한 신생아를 대상으로 시행한 TMS의 위양성율을 조사하고 관련된 요인들을 분석하였다. 총 검사 대상자 중 남아는 8,942명(51.7%), 여아는 8,350명(48.3%)이었고, 평균 제태연령은 $38.6{\pm}1.7$주 였다. 평균 출생 체중은 $3,155.6{\pm}502.4g$, 평균 출생 신장은 $49.1{\pm}2.9cm$, 평균 체질량지수는 $13.0{\pm}3.8kg/m^2$ 이었다. 질식 분만은 9,713 (56.2%)건, 제왕절개는 7,579 (43.8%)건이었다. 평균 검사 시행일은 $2.8{\pm}1.1$일이었다. 위양성을 포함하여 TMS에서 양성으로 확인된 224명은 전체 대상자의 1.3%였다. 다중 로지스틱 회귀분석을 통해 분석한 결과 27주 이하의 초미숙아에서 위양성으로 나타날 odds ratio가 6.957 (95% CI: 1.273-38.008), 1,000 g 이하의 극저체중아에서 5.616 (95% CI: 1.134-27.820)로 나타났다. 위양성 대상자들 중에서는 지방산 대사이상 질환(fatty acid oxidation dis-orders)이 104명(47%), 아미노산 이상질환(amino-acidopathy)가 75명(34%), 유기산 이상 질환(organic aciduria)가 43명(19%)이었다. 전국적인 자료를 모아 평가한다면 국내 발생률에 대한 정확한 평가 및 접근이 가능할 것으로 생각한다.

• 한국컴퓨터정보학회논문지
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• 제14권5호
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• pp.65-75
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• 2009

IT의 발전으로 많은 컴퓨터 사용자들이 인터넷 사용을 생활화하고 있다. HTML 기술을 이용한 웹 기술의 발전은 현대인들의 정보를 빠르고 쉽게 공유할 수 있도록 하고 있으며, 그 이용이 기하급수적으로 증가하고 있는 추세이다. 그러나 그에 따른 부작용으로 중요 시스템에 대한 정보 유출, 전산망 침해 등과 같은 침입 행위 또한 빠른 속도로 증가하고 있다. 이에 본 논문에서 제안하는 공격 트래픽 탐지 기법은 전통적인 네트워크기반 공개 침입탐지시스템인 Snort 탐지한 공격 트래픽 중 false positive 가능성이 있는 패킷을 Nmap 정보를 이용하여 필터링하고, nessus 취약점 정보를 이용하여 2차 필터링을 실시한 후, 운영체제의 적합성, 시그너처 위험도, 보안 취약점을 고려하여 상관성 분석을 최종적으로 실시하여 false positive 경고 메시지를 줄이고 false positive에 의한 오류를 최소화하여 전체적인 공격 탐지 결과를 높였다.

• 전기전자학회논문지
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• 제9권2호
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• pp.108-114
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• 2005

현재 전자우편은 정보통신 사회의 중요한 의사소통 수단이 되고 있으나, 스팸 메일의 증가로 인해 각종 사회 문제를 발생시키고 있다. 스팸 차단을 위해 관련 기관 및 기업이 다양한 형태의 스팸 차단 기술을 개발하고 있으나, 다양한 스팸 유형에 따른 대응이 각각 이루어져야 하므로 많은 처리 비용과 시스템의 복잡도가 가중되고 있다. 그리고 스팸 차단 기술 적용 시, 스팸 방지 필터의 적용 순서에 따라 차단 오인율(False-Positive Error)이 달라져 이용 신뢰도에 큰 영향을 끼친다. 본 논문에서는 스팸 차단 필터의 이용 신뢰도를 향상시키기 위해, Privacy 정보 기반의 False-Positive 메일 복구 기법을 제안 및 구현하였다. 구현된 프로토 타입을 통해 False-Positive 메일 복구 과정을 검증하고, 처리 결과를 분석 및 요약한다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권6호
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• pp.658-665
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• 2000

Purpose : The present study was carried out to determine the diagnostic usefulness of bone scan for evaluating jaw bone extension of oral cancer. Materials and Methods : Medical records, preoperative bone scans, computerized tomographic (CT) scans, conventional radiographs, and findings of histopathologic sections of twenty patients who had been treated for oral malignant tumors by a resection of mandible and soft tissue at Chonnam University Hospital from January, 1994 to September, 1999 were analyzed. Results : In 13 cases which showed histopathologically positive, preoperative bone scans were positive in 12 (92.3%) and false negative in 1 (7.7%). Preoperative CT scans were positive in 9 (69.2%) and false negative in 4 (30.8%) of the 13 cases. Preoperative conventional radiographs were positive in 8 (61.5%) and false negative in 5 (38.5%) of the 13 cases. In 7 cases showing negative histopathologic findings, 1 (14.3%) was in CT scans and 2 (28.6%) were false positive in preoperative conventional radiographs. Conclusion : These results suggest that bone scan is more sensitive and reliable method for evaluating jaw bone extension of oral cancer than conventional radiographs or CT scans.

• 대한방사선기술학회지:방사선기술과학
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• 제44권6호
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• pp.635-643
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• 2021

The results of empirical researches on the diagnosis of lung cancer are insufficient, so it is limited to objectively judge the clinical possibility and utilization according to the accuracy of diagnosis. Thus, this study retrospectively analyzed the lung cancer diagnostic performance of PET-MRI (Positron Emission Tomography-Magnetic Resonance Imaging) by using the decision matrix. This study selected and experimented total 165 patients who received both hematological CEA (Carcinoembryonic Antigen) test and hybrid PET-MRI (¹⁸F-FDG, 5.18 MBq/kg / Body TIM coil. VIVE-Dixon). After setting up the result of CEA (positive:>4 ㎍/ℓ. negative:<2.5㎍/ℓ) as golden data, the lung cancer was found in the image of PET-MRI, and then the SUV_max (positive:>4, negative:<1.5) was measured, and then evaluated the correlation and significance of results of relative diagnostic performance of PET-MRI compared to CEA through the statistical verification (t-test, P>0.05). Through this, the PET-MRI was analyzed as 96.29% of sensitivity, 95.23% of specificity, 3.70% of false negative rate, 4.76% of false positive rate, and 95.75% of accuracy. The false negative rate was 1.06% lower than the false positive rate. The PET-MRI that significant accuracy of diagnosis through high sensitivity and specificity, and low false negative rate and false positive rate of lung cancer, could acquire the fusion image of specialized soft tissue by combining the radio-pharmaceuticals with various sequences, so its clinical value and usefulness are regarded as latently sufficient.

• Clinical and Experimental Pediatrics
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• 제64권5호
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• pp.208-222
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• 2021

The publication of genetic epidemiology meta-analyses has increased rapidly, but it has been suggested that many of the statistically significant results are false positive. In addition, most such meta-analyses have been redundant, duplicate, and erroneous, leading to research waste. In addition, since most claimed candidate gene associations were false-positives, correctly interpreting the published results is important. In this review, we emphasize the importance of interpreting the results of genetic epidemiology meta-analyses using Bayesian statistics and gene network analysis, which could be applied in other diseases.