• Microbiology and Biotechnology Letters
• /
• v.20 no.4
• /
• pp.483-490
• /
• 1992

In order to understand the transfer behavior of a particular gene in water environments, kanamycin resistance ($Km^r$) gene was tracked by Southern hybridization with DNA probe in its conjugal transfer. A $Km^r$ natural bacterial isolate and genetically modified microorganisms (GMMs) constructed from the isolate were used as donor for conjugal transfer of the $Km^r$ gene. The transfer frequencies of the $Km^r$ gene from GMM strains were generally 10 to 100 times higher than those from the natural isolate. The conjugants obtained from GMM strains in LB broth had more plasmids newly appeared, and particularly the conjugants in A Wand FW waters revealed more rearrangement in their plasmids as a function of conjugation time. When plasmids of the conjugants obtained in LB broth were Southern hybridized with DNA probe of the $Km^r$ gene, the $Km^r$ plasmids in the conjugants were detected at the same position of the plasmids in donor cells, in spite of the fact that the plasmids were highly rearranged in conjugant cells. But the $Km^r$ plasmids in the donor of DKI and DKC601, and DKC600 were not identified in the conjugants obtained after 50 h conjugation in AW and after 30 h in AW, respectively. The size of the $Km^r$ plasmids showing hybridization signal were a little changed in the conjugants obtained in A Wand FW waters. Therefore, the method of Southern hybridization with DNA probe was proved to be very specific and useful for tracking of particular genes in water environments.

• Proceedings of the KSME Conference
• /
• 2004.04a
• /
• pp.1869-1874
• /
• 2004

Acoustic pressure field around the cross-flow fan is predicted by a hydrodynamic-acoustic splitting method. Unsteady flow field is obtained by solving the incompressible Navier-Stokes equations using an unstructured finite-volume method on the triangular meshes, while the acoustic waves generated inside the cross-flow fan are predicted by solving the perturbed compressible equations(PCE) with a 6th-order compact finite difference method. Computational results show that the acoustic waves of BPF tone are generated by interactions of the blades wakes with the stabilizer, which then are reflected from the rear-guider and mainly propagate towards the fan inlet. Also, a directivity of BPF noise predicted by the PCE is noticeably different from that of the FW-H equations, in which a fan casing effect cannot be included.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.6 no.2
• /
• pp.81-92
• /
• 2001

According to analyses of high-resolution seismic profiles (air gun, sparker, and SBP) and a deep-drill core(YSDP 105) in the mid-eastern Yellow Sea, stratigraphic and geoacoustic models have been established and seismo-acoustic modeling has been fulfilled using ray tracing of finite element method. Stratigraphic model reflects seismo-, litho-, and chrono-stratigraphic sequences formed under a significant influence of Quaternary glacio-eustatic sea-level fluctuations. Each sequence consists of terrestrial to very-shallow-marine coarse-grained lowstand systems tract and tidal fine-grained transgressive to highstand systems tract. Based on mean grain-size data (121 samples) of the drill core, bulk density and P-wave velocity of depositional units have been inferred and extrapolated down to a depth of the recovery using the Hamilton's regression equations. As goo-acoustic parameters, the 121 pairs of bulk density and P-wave velocity have been averaged on each unit of the stratigraphic model. As a result of computer ray-tracing simulation of the subsurface strata, we have found that there are complex ray paths and many acoustic-shadow zones owing to the presence of irregular layer boundaries and low-velocity layers.

• Journal of the Korean Vacuum Society
• /
• v.15 no.3
• /
• pp.325-330
• /
• 2006

In order to verify the integrity of the first wall (FW) of the International Thermonuclear Experimental Reactor (ITER), the fabricated Cu/SS mock-up is tested in the JAEA Electron Beam Irradiation Test Stand (JEBIS). To fabricate the Cu/SS mock-up, CuCrZr and 316L authentic stainless steel (SS316L) are used for Cu alloy and steel, respectively The hot isostatic pressing (HIP) is used as a manufacturing method with a $1050^{\circ}C$ and 150 MPa. The high heat flux (HHF) test is performed using an electron beam with a heat flux of $5MW/m^2$ and a cycle of 15-sec on time and 30-sec off time. The temperature measurement in the HHF test shows good agreement with the results obtained from ANSYS code analysis, which is used for determining the HHF test conditions.

• Korean Journal of Crystallography
• /
• v.9 no.1
• /
• pp.71-76
• /
• 1998

Crystal structure of Bis(ethylenediamine)palladium(II)-Bis(oxalato)palladate(II0 has been determined by X-ray crystallography. Crystal data : (Pd(C2H8N2)2.Pd(C2O4)2), Fw=509.04, Monocline, Space Group P21/c (no=14), a=6.959(2), b=13.506(2), c=15.339(2) Å, β=99.94(3), Z=4, V=1420 Å3, Dc=2.380 gcm-3, μ=25.46cm-1, F(000)=992. The intensity data were collected with Mo-Kα radiation (λ=0.7107 Å) on an automatic four-circle diffractometer with a graphite monochromater. The structure was solved by Patterson method and refined by full matrix least-square methods using unit weights. The final R and S values were R=0.021, Rw=0.030, Rall=0.032 abd S=2.1 for 1472 observed reflections. The essentially planar complex anions form diade of interplanar distances of 3.41 Å and their diads are stacked along aaxis with interplanar separation of 3.44 Å.

• The Journal of the Acoustical Society of Korea
• /
• v.38 no.3
• /
• pp.321-327
• /
• 2019

The purpose of this study is paper is to improve the flow performance and to reduce the aerodynamic noise of air discharge system consisting of a centrifugal fan, ducts and a housing for the clothes dryer. Using computational fluid dynamics and acoustic analogy based on FW-H (Ffowcs-Williams and Hawkings) Eq., air flow field and acoustic fields of the air discharge system are investigated. To optimize aerodynamic performance and aerodynamic noise, the response surface method is employed. The two factors central composite design using the inflow and outflow angles of fan blades is adopted. The devised optimum design shows the reduction of turbulent kinetic energy in the ducts and the housing of the system, and as a result, the improved flow rate and reduce noise is confirmed. Finally, the experment using the proto-type manufactured usign the optimum design shows the increase of flow rate by 4.2 %.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2002.05a
• /
• pp.1005-1009
• /
• 2002

The objective of this research is to suggest the noise prediction method of the centrifugal compressor. It is focused on the Blade Passing Frequency (BPF) component which is regarded as the main part of the rotating impeller noise. Euler solver is used to simulate the flow-field of the centrifugal compressor and time-dependent pressure data are calculated to perform the near-field noise prediction by Ffowcs Williams-Hawkings (FW-H) formulation. Indirect Boundary Element Method (IBEM) is applied to consider the noise propagation effect. Pressure fluctuations of the inlet and the outlet in the centrifugal compressor impeller are presented and Sound Pressure Level (SPL) prediction results are compared with the experimental data.

• 유체기계공업학회:학술대회논문집
• /
• 2004.12a
• /
• pp.567-571
• /
• 2004

The 2-dimensional unsteady flows on and around the cambered airfoils were computed by applying LES with the deductive dynamic SGS model. The unsteady flow field were used as inputs to compute the far-field sounds and directivity patterns from rotating blades by a hybrid approach that exploits Farassat's formula. The BEM (Boundary Element Method) was applied to predict the frequency characteristics from the rotating blades for the cases of even- and uneven-pitched fans. The BEM results suggested that the unevenly pitched fan have less pronounced discrete peaks at BEF frequencies, which was confirmed by the experiment.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.29 no.5
• /
• pp.512-519
• /
• 2023

Noise pollution poses significant challenges to human well-being and marine ecosystems. It is primarily caused by the flow around ships and marine installations, emphasizing the need for accurate noise evaluation of flow noise to ensure environmental safety. Existing flow noise analysis methods for underwater environments typically use a hybrid method combining computational fluid dynamics and Ffowcs Williams-Hawkings acoustic analogy. However, this approach has limitations, neglecting near-field effects such as reflection, scattering, and diffraction of sound waves. In this study, an alternative using direct method flow noise analysis via the lattice Boltzmann method (LBM) is incorporated. The LBM provides a more accurate representation of the underwater structural boundaries and acoustic wave effects. Despite challenges in underwater environments due to numerical instabilities, a novel DM-TS LBM collision operator has been developed for stable implementations for hydroacoustic applications. This expands the LBM's applicability to underwater structures. Validation through flow noise analysis in pipe orifice demonstrates the feasibility of near-field analysis, with experimental comparisons confirming the method's reliability in identifying main pressure peaks from flow noise. This supports the viability of near-field flow noise analysis using the LBM.

• 유체기계공업학회:학술대회논문집
• /
• 2004.12a
• /
• pp.89-93
• /
• 2004

To numerically construct the sound fields by a plenum fan mostly found in Air-Handling Unit (AHU), the Kirchhoff-BEM approach was applied to the near-field data of a turbo fan. The scattering effects were found to be significant by the plenum box structure for high-frequency components of source. The directivity petterns and sound pressure levels were also dependent upon the helmholts number which must be considered of the design stage for sound reduction program.