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Immunostimulatory and Anti-Obesity Activity of Lonicera insularis Nakai Extracts in Mouse Macrophages RAW264.7 Cells and Mouse Adipocytes 3T3-L1 Cells (섬괴불나무(Lonicera insularis Nakai) 추출물의 면역자극 및 항비만 활성)

  • Yu, Ju Hyeong;Yeo, Joo Ho;Choi, Min Yeong;Lee, Jae Won;Geum, Na Gyeong;An, Mi-Yun;Jeong, Jin Boo
    • Korean Journal of Plant Resources
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    • v.35 no.4
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    • pp.417-427
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    • 2022
  • In this study, we investigated in vitro immuno-stimulatory and anti-obesity activity of fruit (LIF), leaves (LIL) and stems (LIS) from Lonicera insularis Nakai in mouse macrophages RAW264.7 cells and mouse pre-adipocytes 3T3-L1 cells. LIF, LIL and LIS increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and activated phagocytosis in RAW264.7 cells. Inhibition of toll-like receptor 2/4 (TLR2/4) partly blocked LIF, LIL and LIS mediated production of immunostimulatory factors. In addition, inhibition of mitogen-activated protein kinases (MAPK) signaling attenuated the production of immunostimulatory factors induced by LIF, LIL and LIS. Based on these results of this study, LIF, LIL and LIS is thought to activate macrophages the production of immunostimulatory factors and phagocytosis through toll-like receptor 2/4 (TLR2/4) and MAPKs signaling pathway. In anti-obesity study, LIF reduced the lipid accumulation in 3T3-L1 cells. LIF increased the protein phosphorylation expressions such as AMP-activated protein kinase (AMPK), hormone sensitive lipase (HSL), adipose triglyceride lipase (ATGL) related to the lipolysis of the adipocytes. In addition, LIF increased the expression of proteins involved in energy metabolism and brown adipose tissues differentiation such as peroxisome proliferator-activated receptor gamma coativator 1α (PGC-1α) and PR domain-containing16 (PRDM16). These results suggest that LIF is involved in lipid accumulation inhibition through expressing the proteins such as lipolysis and differentiation of white adipocytes to brown adipocytes.

A Study of Anti-wrinkle Activities as a Functional Cosmetic Ingredient of Rhododendron brachycarpum Extracts (만병초(Rhododendron brachycarpum) 추출물의 기능성 화장품 소재로써의 주름개선 활성에 관한 연구)

  • Yeom, Hyeon-Ji;Oh, Min-Jeong;Chae, Jung-Woo;Lee, Jin-Young
    • Journal of Life Science
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    • v.32 no.8
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    • pp.622-632
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    • 2022
  • The purpose of this study was to investigate activities as functional cosmetic materials, focusing on Rhododendron brachycarpum (RB) and Rhododendron fortunei (RF). The tyrosinase inhibitory effect, related to skin-whitening, was 32.6% and 39.3% respectively at the concentration of 1,000 ㎍/ml. The elastase inhibitory effect, related to skin anti-wrinkling activity, was 30% and 36.2% at 1,000 ㎍/ml concentration. Collagenase inhibitory activity showed 77.7%, and 80.2% respectively at 1,000 ㎍/ml concentration, which demonstrated excellent inhibitory activity. For a cell viability test, that measured on fibroblast cells by RB and RF extracts. Furthermore, the cell viability test showed 100.9% and 98.9% with cell viability at 100 ㎍/ml concentration in CCD-986Sk. The protein expression inhibitory effect of RB and RF extracts was measured by western blot at 25, 50, and 100 ㎍/ml concentrations, and the β-actin was used as a positive control. As a result, western blot of RB and RF extracts was measured by the expression inhibition rate of the MMP-1, MMP-2, MMP-3 protein, and was decreased by 67.2%, 65.5%, 13.6%, and 89.1%, 85.0%, and 62.7% at 100 ㎍/ml concentration. The mRNA expression inhibitory effect of RB and RF extracts was measured by RT-PCR at 25, 50, and 100 ㎍/ml concentrations, and the GAPDH was used as a positive control. According to the results of RT-PCR of RB and RF extracts, the expression inhibition rate of the MMP-1, MMP-2, and MMP-3 mRNA was decreased by 70.1%, 9.1%, 37.9%, and 38.2%, 8.3%, 57.3% at 100 ㎍/ml concentrations. So, RB and RF extracts showed the anti-wrinkle effectiveness as a functional cosmetic material.

A Study on the Demand for Cultural Ecosystem Services in Urban Forests Using Topic Modeling (토픽모델링을 활용한 도시림의 문화서비스 수요 특성 분석)

  • Kim, Jee-Young;Son, Yong-Hoon
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.4
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    • pp.37-52
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    • 2022
  • The purpose of this study is to analyze the demand for cultural ecosystem services in urban forests based on user perception and experience value by using Naver blog posts and LDA topic modeling. Bukhansan National Park was used to analyze and review the feasibility of spatial assessments. Based on the results of topic modeling from blog posts, a review process was conducted considering the relevance of Bukhansan National Park's cultural services and its suitability as a spatial assessment case, and finally, an index for the spatial assessment of urban forest's cultural service was derived. Specifically, 21 topics derived through topic analysis were interpreted, and 13 topics related to cultural ecosystem services were derived based on the MA(Millennium Ecosystem Assessment)'s classification system for ecosystem services. 72.7% of all documents reviewed had data deemed useful for this study. The contents of the topic fell into one of the seven types of cultural services related to "mountainous recreation activities" (23.7%), "indirect use value linked to tourism and convenience facilities" (12.4%), "inspirational activities" (11.2%), "seasonal recreation activities" (6.2%), "natural appreciation and static recreation activities" (3.7%). Next, for the 13 cultural service topics derived from data gathered about Bukhansan National Park, the possibility of spatial assessment of the characteristics of cultural ecosystem services provided by urban forests was reviewed, and a total of 8 cultural service indicators were derived. The MA's cultural service classification system for ecosystem services, which was widely used in previous studies, has limitations in that it does not reflect the actual user demand of urban forests, but it is meaningful in that it categorizes cultural service indicators suitable for domestic circumstances. In addition, the study is significant as it presented a methodology to interpret and derive the demand for cultural services using a large amount of user awareness and experience data.

Review of the Weather Hazard Research: Focused on Typhoon, Heavy Rain, Drought, Heat Wave, Cold Surge, Heavy Snow, and Strong Gust (위험기상 분야의 지난 연구를 뒤돌아보며: 태풍, 집중호우, 가뭄, 폭염, 한파, 강설, 강풍을 중심으로)

  • Chang-Hoi Ho;Byung-Gon Kim;Baek-Min Kim;Doo-Sun R. Park;Chang-Kyun Park;Seok-Woo Son;Jee-Hoon Jeong;Dong-Hyun Cha
    • Atmosphere
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    • v.33 no.2
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    • pp.223-246
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    • 2023
  • This paper summarized the research papers on weather extremes that occurred in the Republic of Korea, which were published in the domestic and foreign journals during 1963~2022. Weather extreme is defined as a weather phenomenon that causes serious casualty and property loss; here, it includes typhoon, heavy rain, drought, heat wave, cold surge, heavy snow, and strong gust. Based on the 2011~2020 statistics in Korea, above 80% of property loss due to all natural disasters were caused by typhoons and heavy rainfalls. However, the impact of the other weather extremes can be underestimated rather than we have actually experienced; the property loss caused by the other extremes is hard to be quantitatively counted. Particularly, as global warming becomes serious, the influence of drought and heat wave has been increasing. The damages caused by cold surges, heavy snow, and strong gust occurred over relatively local areas on short-term time scales compared to other weather hazards. In particularly, strong gust accompanied with drought may result in severe forest fires over mountainous regions. We hope that the present review paper may remind us of the importance of weather extremes that directly affect our lives.

Physiological and Growth Responses of M. thunbergii to Different Levels of Fertilization (시비처리에 따른 후박나무의 생리 및 생장 반응)

  • Jung-Won Sung;Yeong Geun Song;Haeun Koo;Hyeon Hwa Kim;Se Min Byun;Chae Rim Lee;Seok-Gon Park;Kyeong Cheol Lee
    • Korean Journal of Plant Resources
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    • v.36 no.2
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    • pp.172-180
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    • 2023
  • In the current study, four groups; control, 500, 1000, and 2000 mg/L, were treated to investigate the effects of physiological and growth characteristics on Machilus thunbergii under various fertilization levels. As a result of the physiological response to the fertilization treatment, the fertilized group demonstrated relatively higher levels of A, ITE, WUEi, Vcamx, PIabs, and SFIabs in comparison to the control. The best photosynthetic mechanism was most clearly seen at 1000 mg/L, which involved gas exchange through active stomatal opening and closing. For a productive photosynthetic mechanism. As seen in the growth response of M. thunbergii to fertilization treatment, the fertilized group has significantly higher height, DRC, leaf dry weight, stem dry weight, total dry weight, LWR, and SWR than the control group. A healthy seedling quality index was particularly evident at 1000 mg/L, and other growth indicators were also at a decent level. 500 mg /L also demonstrated growth characteristics that were comparable to those at 1000 mg/L. As a result, M. thunbergii featured the best physiological and growth characteristics in response to the fertilization treatment at 1000 mg/L, and 500 mg/L also showed a similar trend, which is considered to be a good option from an economical perspective.

Predicting the Effects of Agriculture Non-point Sources Best Management Practices (BMPs) on the Stream Water Quality using HSPF (HSPF를 이용한 농업비점오염원 최적관리방안에 따른 수질개선효과 예측)

  • Kyoung-Seok Lee;Dong Hoon Lee;Youngmi Ahn;Joo-Hyon Kang
    • Journal of Wetlands Research
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    • v.25 no.2
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    • pp.99-110
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    • 2023
  • Non-point source (NP) pollutants in an agricultural landuse are discharged from a large area compared to those in other land uses, and thus effective source control measures are needed. To develop appropriate control measures, it is necessary to quantify discharge load of each source and evaluate the degree of water quality improvement by implementing different options of the control measures. This study used Hydrological Simulation Program-FORTRAN (HSPF) to quantify pollutant discharge loads from different sources and effects of different control measures on water quality improvements, thereby supporting decision making in developing appropirate pollutant control strategies. The study area is the Gyeseong river watershed in Changnyeong county, Gyeongsangnam-do, with agricultural areas occupying the largest proportion (26.13%) of the total area except for the forest area. The main pollutant sources include chemical and liquid fertilizers for agricultural activities, and manure produced from small scale livestock facilities and applied to agriculture lands or stacked near the facilities. Source loads of chemical fertilizers, liquid fertilizers and livestock manure of small scale livestock facilities, and point sources such as municipal wastewater treatment plants (WWTPs), community WWTPs, private sewage treament plants were considered in the HSPF model setup. Especially, NITR and PHOS modules were used to simulate detailed fate and transport processes including vegitation uptake, nutrient deposition, adsorption/desorption, and loss by deep percolation. The HSPF model was calibrated and validated based on the observed data from 2015 to 2020 at the outlet of the watershed. The calibrated model showed reasonably good performance in simulating the flow and water quality. Five Pollutants control scenarios were established from three sectors: agriculture pollution management (drainge outlet control, and replacement of controlled release fertilizers), livestock pollution management (liquid fertilizer reduction, and 'manure management of small scale livestock facilities) and private STP management. Each pollutant control measure was further divided into short-term, mid-term, and long-term scenarios based on the potential achievement period. The simulation results showed that the most effective control measure is the replacement of controlled release fertilizers followed by the drainge outlet control and the manure management of small scale livestock facilities. Furthermore, the simulation showed that application of all the control measures in the entire watershed can decrease the annual TN and TP loads at the outlet by 40.6% and 41.1%, respectively, and the annual average concentrations of TN and TP at the outlet by 35.1% and 29.2%, respectively. This study supports decision makers in priotizing different pollutant control measures based on their predicted performance on the water quality improvements in an agriculturally dominated watershed.

Analysis of the impact of mathematics education research using explainable AI (설명가능한 인공지능을 활용한 수학교육 연구의 영향력 분석)

  • Oh, Se Jun
    • The Mathematical Education
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    • v.62 no.3
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    • pp.435-455
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    • 2023
  • This study primarily focused on the development of an Explainable Artificial Intelligence (XAI) model to discern and analyze papers with significant impact in the field of mathematics education. To achieve this, meta-information from 29 domestic and international mathematics education journals was utilized to construct a comprehensive academic research network in mathematics education. This academic network was built by integrating five sub-networks: 'paper and its citation network', 'paper and author network', 'paper and journal network', 'co-authorship network', and 'author and affiliation network'. The Random Forest machine learning model was employed to evaluate the impact of individual papers within the mathematics education research network. The SHAP, an XAI model, was used to analyze the reasons behind the AI's assessment of impactful papers. Key features identified for determining impactful papers in the field of mathematics education through the XAI included 'paper network PageRank', 'changes in citations per paper', 'total citations', 'changes in the author's h-index', and 'citations per paper of the journal'. It became evident that papers, authors, and journals play significant roles when evaluating individual papers. When analyzing and comparing domestic and international mathematics education research, variations in these discernment patterns were observed. Notably, the significance of 'co-authorship network PageRank' was emphasized in domestic mathematics education research. The XAI model proposed in this study serves as a tool for determining the impact of papers using AI, providing researchers with strategic direction when writing papers. For instance, expanding the paper network, presenting at academic conferences, and activating the author network through co-authorship were identified as major elements enhancing the impact of a paper. Based on these findings, researchers can have a clear understanding of how their work is perceived and evaluated in academia and identify the key factors influencing these evaluations. This study offers a novel approach to evaluating the impact of mathematics education papers using an explainable AI model, traditionally a process that consumed significant time and resources. This approach not only presents a new paradigm that can be applied to evaluations in various academic fields beyond mathematics education but also is expected to substantially enhance the efficiency and effectiveness of research activities.

A Study on the Pattern and Promotion of Landscape Architects Participation in Urban Regeneration Projects- Focusing on the Urban Regeneration New Deal Project (2017-2019) - (도시재생사업에서 조경의 참여 양상과 증진 모색 - 도시재생 뉴딜 선정사업(2017-2019)을 중심으로 -)

  • Lee, Jeong-Hee;Yun, Young-Jo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.50 no.6
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    • pp.96-109
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    • 2022
  • This study aims to identify the role and the use of landscape architects as a core strategy in urban regeneration projects by project type. This study attempted to find ways to promote landscape architects' participation patterns and characteristics in promoting urban regeneration and revitalization plans. Among the New Deal projects, from 2017 to 2019, when the urban regeneration projects were promoted, a total of 822 projects with terms such as park, garden, landscaping, rest space, forest, greening, planting, and flowers were included, In this study, an integrated approach was applied, considering the status of landscape architects participation, a literature survey by project entity, analysis of prior research, revitalization plan reports, and interviews by project entity, and aspects and characteristics of landscape architects participation according to project type. The results were classified. In the urban regeneration projects, the landscape characteristics were analyzed in terms of hardware or software participation methods according to the project type, and the participation patterns and promotion measures of landscape architecture and ways to promote them identified in the course of the project are as follows. First, the role and participation pattern of landscape architects in urban regeneration projects differed depending on the type of project. As the prerequisites for each type of project, the subject of the project, the direction, the differentiation of the regeneration strategy for each project, and the level of participation of residents interacted in a complex way. The participation of landscape architects has changed and expanded its meaning and role. Second, the change and value of the expanded the participation method of landscape architects in the urban regeneration project and appeared as a multi-purpose and complex role in the process of a project, indicating that the value of landscape in the classical sense is changing and its substantive aspect depends on the type of project. Third, this study confirmed the importance of recognizing the role and value of 'landscape architecture' as a strategic and practical means of regeneration that can be utilized. Therefore, in order to promote the participation of landscape architects, diversification is required through changes in participation methods according to urban regeneration strategies for each project, rather than participation focused on hardware projects, such as park creation in terms of form and place. In addition, it was confirmed that the participation of landscape architects, which play an important role in fostering a sense of community among residents as an experience and activity program, should be further activated in terms of the process of the ideal way of existence. This study is meaningful in that it substantially grasps that the role of landscape architects is expanding as the subject of urban regeneration projects, and presents the direction for its promotion. Participation and research should be continued.

Management Planning of Wind Corridor based on Mountain for Improving Urban Climate Environment - A Case Study of the Nakdong Jeongmaek - (도시환경개선을 위한 산림 기반 바람길 관리 계획 - 낙동정맥을 사례로 -)

  • Uk-Je SUNG;Jeong-Min SON;Jeong-Hee EUM;Jin-Kyu MIN
    • Journal of the Korean Association of Geographic Information Studies
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    • v.26 no.1
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    • pp.21-40
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    • 2023
  • This study analyzed the cold air characteristics of the Nakdong Jeongmaek, which is advantageous for the formation of cold air that can flow into the city, in order to suggest the wind ventilation corridor plans, which have recently been increasing interest as a way to improve the urban thermal environment. In addition, based on the watershed analysis, specific cold-air watershed areas were established and management plans were suggested to expand the cold air function of the Nakdong Jeongmaek. As a result of the analysis of cold air in the Nakdong Jeongaek, cold air was strongly generated in the northern forest of the Jeongamek, and flowed into nearby cities along the valley topography. On average, the speed of cold air was high in cities located to the east of the Jeongmaek, while the height of cold air layer was high in cities located to the west. By synthesizing these cold air characteristics and watershed analysis results, the cold-air watershed area was classified into 8 zones, And the plans were proposed to preserve and strengthen the temperature reduction of the Jeongmaek by designating the zones as 'Conservation area of Cold-air', 'Management area of Cold-air', and 'Intensive management area of Cold-air'. In addition, in order to verify the temperature reduction of cold air, the effect of night temperature reduction effect was compared with the cold air analysis using weather observation data. As a result, the temperature reduction of cold air was confirmed because the night temperature reduction was large at the observation station with strong cold air characteristics. This study is expected to be used as basic data in establishing a systematic preservation and management plan to expand the cold air function of the Nakdong Jeongmaek.

A Study on the Characteristic of Habitat and Mating Calls in Korean Auritibicen intermedius (Hemiptera: Cicadidae) Using Bioacoustic Detection Technique (생물음향탐지기법을 활용한 한국 참깽깽매미 서식 및 번식울음 특성 연구)

  • Yoon-Jae Kim;Kyong-Seok Ki
    • Korean Journal of Environment and Ecology
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    • v.36 no.6
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    • pp.592-602
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    • 2022
  • This study aimed to check habitat distribution and analyze influencing factors by analyzing the mating calls of Auritibicen intermedius inhabiting limited locations in South Korea by applying bioacoustic detection techniques. The study sites were 20 protection areas nationwide. The mating call analysis period was 4 years from 2017 to 2021, excluding 2020. The bioacoustic recording system installed at each study site collected recordings of mating calls every day for 1 minute per hour. Climate data received from the Meteorological Agency, such as temperature, humidity, rainfall, cloudiness, and sunshine, were analyzed. The results of this study identified A. intermedius habitat only in four national parks in the highlands of Gangwon Province (Mt. Seorak, Mt. Odae, Mt. Chiak, and Mt. Taebak) out of 20 study sites. During the four years of study, the mating call period of A. intermedius was between August 5 and September 28, and the duration of the mating call was 31 to 52 days. The temperature analysis during the appearance period of A. intermedius showed that A. intermedius mainly produced mating calls at temperatures between 13.1℃ and 35.3℃, and the average temperature during the circadian cycle of mating calls (09:00 to 16:00) was 24.4 to 24.9℃. The analysis of the circadian cycle of mating calls at four study sites where A. intermedius appeared in 2019 showed that A. intermedius produced mating calls from 06:00 to 16:00 and that they peaked around 11:00 to 12:00. During the appearance period of A. intermedius, four species appeared in common: Hyalessa maculaticollis, Meimuna opalifera, Graptopsaltria nigrofuscata, and Suisha coreana. A logistic regression analysis confirmed that sunlight was the environmental factor affecting the mating call of A. intermedius. Regarding interspecific influence, it was confirmed that A. intermedius exchanged interspecific influence with 4 other common species (H. maculaticollis, M. opalifera, G. nigrofuscata, and S. coreana). The above results confirmed that A. intermedius habitats were limited in the highlands of Gangwon Province highlands in Korea and produced mating calls at a lower temperature compared to other species. These results can be used as basic data for future research on A. intermedius in Korea.