• Applied Biological Chemistry
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• v.34 no.2
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• pp.134-141
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• 1991

In order to investigate the changes and characteristics of biochemical metabolic substances of soybean tissue culture during the cultural period, immature cotyledons were detached form the plant on 15th days after flowering and cultured in vitro for 3 weeks. The cultures were classified into embryogenic(EC) and non-embryogenic callus(NEC). A part of the EC lines were subcultured for another 3 weeks and classified into root forming(RFC), and shoot forming cultures(SFC). Another part of the EC lines were used for isolation of protoplasts, which were subsequently cultured in vitro for 4 weeks. The cultures were classified into embryogenic(PEC) and non-embryogenic callus(PNEC) derived from the protoplasts. The cultures of EC and PEC lines showed higher phenylalanine content and lower methionine content than those of NEC and PNEC. At organ differentiation stage, both cultures showed the content of aspartic acid decreased, while the other amino acids increased as a whole. The protein pattern analysis of the cultures revealed that EC and NEC lines contained distinctive polypeptides, with mass of ca. 18KD for EC and ca. 22KD for NEC respectively. The EC and PEC lines also showed high activity of peroxidase isozyme A(piA), while the RFC and SFC lines showed that of peroxidase isozyme B(piB).

• Magazine of the Korean Society of Agricultural Engineers
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• v.27 no.1
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• pp.62-70
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• 1985

The purpose of this study is to investigate the basic data such the total, the daily maximum, and the peak stage of consumptive use of water and also the soil moisture extraction pattern for irrigation plan of tobacco during the growing period. The plots at which this study was conducted are divided into three fertilization levels of 30g, 60g, and 90g. Each block for three levels is divided as vinyl mulching and irrigation plot, vinyl mulching and nonirrigation plot, and nonmulching and irrigation plot. The results obtained are summarized as follows: 1. The evapotranspiration amount of mulching-irrigation plots are similar to that of mulching-nonirrigation plots. While, the evapotranspiration amount of mulching plots are different obviousely from that of nonmulching plots. Therefore, a significance was recognized between the mulching plots and the nonmulching plots. 2. The amount of evapotranspiration in case of 60g and 90g fertilization level was larger than that of 30g. But the 60g plots and the 90g plots showed little differences. 3. In the total amount of evapotranspiration for each of the experimental plots during the growing period, nonmulching-irrigation plot showed the largest value of 332.9mm, second the mulching-irrigation plot, 284. 9mm, and the mulching-nonirrigation plot, the smallest as 255. 9mm. 4. In the monthly average amount of evapotranspiration for each of the treatment plots, the mulching-irrigation the mulching-nonirrigation, and the nonmulching-irrigation plot showed 3. 6mm, 3. 2mm and 4. 2mm respectively. The daily maximum amount of evapotranspiration showed 5. 1mm, 4. 5mm, and 6.4mm for the mulching-irrigation, the mulching-nonirrigationl, and the nonmulching-irrigation plot respectively. 5. It was confirmed that the higher correlationship exists between the weight of dried leaves and the amount of evapotranspiration, and between the weight of dried leaves and the coefficient of evapotranspiration with the function of logarithms. The coefficient of evapotranspiration have a tendency to increase in proportion to the leaf area index. 6. The maximum coefficient of evapotranspration and the largest leaf area index showed 1. 45 and 5.5 respectively. The stage appeared maximum values was assumed to be before and after flowering. 7. The soil moisture extraction pattern has changed by the depth of root zone for the tobacco's growing. The soil moisture extraction influenced on the 20cm depth of soil after 15 days passed, the 30cm depth after 25 days passed and the whole root zone after 45 days passed from planting. It was shown in the only mulching-irrigation plot after S5days passed from planting that the rate of soil moisture extraction of 20cm layer was larger than that of 10cm layer.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.1
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• pp.55-60
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• 2007

This study was conducted to find characteristics of photosynthesis for dwarf and street tree cultivars of Hibiscus syriacus L. Characteristics of growth and flowering on some cultivars were investigated. The photosynthetic capacity for the cultivars was also measured. Bulsae showed the best height increase; Soyang represented the lowest growth. Baektanshim ${\times}$ Kyungbuk1 had the largest number of branches and leaves. Sundeok and Bulsae were the best in terms of height and width of flower, Soyang showed the smallest flowers. According to the analysis of chlorophyll content (chlorophyll a, b, total) for the cultivars, there was little difference between Sundeok and Bulsae. Based on light response curves for cultivars, it was found that Baektanshim ${\times}$ Kyungbuk1 and Soyang had lower light compensation and light saturation points than Bulsae and Sundeok. Cultivars Baektanshim ${\times}$ Kyungbuk1 and Soyang were also low in dark respiration, photosynthetic capacity, and net apparent quantum yield. In conclusion, it is recommended that cultivars Baektanshim ${\times}$ Kyungbuk1 and Soyang might be used as a dwarf type due to photosynthetic capacity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.7
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• pp.593-598
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• 2006

This study was conducted to investigate the effect of tillage methods such as plowing and rotary tillage (PRT), rotary tillage (RTG), no-tillage after barley straw application (NTB), and barley straw mulching after plowing and rotary tillage (BPR) on the growth and the yield of soybean when cultivated after the cultivation of barley. The methods were compared with the control method in which plowing and rotary tillage after barley straw incineration was applied. Barley straw application resulted in increase in organic matter, total nitrogen, phosphate, and exchangeable cation regardless of tillage methods. Porosity and moisture level in paddy soil was ranked as follows : PRT > RTG > BPR > control > NTB. Decomposition rate of barley straw dramatically increased to 41.7% toward 30 days after soybean sowing, higher in NTB, DRB, and RTG than in BPR. Weed occurrence was decreased 36% in NTB and 40% in BPR. Root activity, nodulation and the dry weight per plant of soybean at flowering stage were highest in NTB and lowest in PRT. Soybean yield in NTB was 3,070 kg/ha increasing 19%, whereas that in PRT was not increased. Therefore in case of a frequent rain during the cultivation of soybean in paddy field PRT could result in excess moisture level in soil, the cultivation without tillage is desirable.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.3
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• pp.128-134
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• 1987

This experiment was conducted to evaluate the effects of cutting in field and solution culture. Periodical samplings of alfalfa in cutting and uncutting plots were taken to measure nodule development and nodule activity. Regrowth of plant and nodule development after shoot cutting by different heights and nodule removal at different levels were investigated in solution culture of alfalfa plant. 1. Nodule weight in the field was reduced 30% after the first cutting and 25% after the second cutting, but during the following 30 days after second cutting, there was no significant difference between cutting and uncutting plots. 2. Specific nodule activities of cutting plots at the beginning of June and at the beginning of September were 80% and loo%, higher than those of uncutting plots respectively. Total nodule activities of cutting plots in late August and early September were 40% higher than those of uncutting plot. The decrease of nodule activity can be prevented by cutting at flowering stage. 3. The decrease of nodules in solution culture when 50% of the shoot was cut, was as much as that when shoot was not cut or flower buds were removered. But when 90% of the shoot was cut, the number of the nodules were decreased more remarkably than the above treatments. New nodules, when 90% of the shoot was cut, were reformed slowly and did not grow fully until 15 days after cutting.

• Korean Journal of Organic Agriculture
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• v.11 no.4
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• pp.103-115
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• 2003

In order to investigate the possibility for use to control environmental pollution, Kenaf(Hibiscus cannabinus L.) was used to obtain information of their growth and $CO_2$ response under different temperatures and $CO_2$ concentration. The highest percentage of germination and aboveground dry mass of Kenaf were found at 30$^{\circ}C$ and 35$^{\circ}C$ by 89.0% and 3.2g, respectively under different temperatures. The amount of $CO_2$ absorption and aboveground dry mass production of Kenaf were higher than those of maize during the whole growing period and the last sampling of aboveground dry mass of Kenaf and maize were 252.9g and 200.8g, respectively. The highest plant height was found at 400ppm by 131.0cm and the next was in the order of 600ppm by 1293cm, and 800ppm by 108.8cm. Leaf area was higher in the order of 400ppm > 600ppm > 800ppm, whereas leaf dry mass was in the order of 800ppm > 600ppm > 400ppm under different $CO_2$ concentration, showing that leaf became thicker as $CO_2$ concentration was increased. Days from seeding to flowering became shorter by 13 days in 35/25$^{\circ}C$ compared with 25/15$^{\circ}C$ between two temperature regimes and they also became shorter as $CO_2$ concentration was increased. Aboveground dry mass was higher in 35/25$^{\circ}C$ than that of 25/15$^{\circ}C$ between two temperature regimes. while it was increased in the order of 800ppm > 600ppm > 400ppm as CO2 concentration was increased. Temporal changes of leaf dry mass during growth period showed no difference between $CO_2$ concentration in 25/15$^{\circ}C$ , but the highest of it was found at 800ppm in 35/25$^{\circ}C$. The highest temporaI increase of root dry mass was found at 800ppm in 25/15$^{\circ}C$, but 35/25$^{\circ}C$ showed no difference between different $CO_2$ concentration.

• Molecules and Cells
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• v.23 no.2
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• pp.145-153
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• 2007

Elucidation of the roles of circadian associated factors requires a better understanding of the molecular mechanisms of circadian rhythms, control of flowering time through photoperiodic pathways, and photosensory signal transduction. In Arabidopsis, the APRR1 quintet, APRRs 1, 3, 5, 7, and 9, are known as central oscillator genes. Other plants may share the molecular mechanism underlying the circadian rhythm. To identify and characterize these circadian response genes in Brassica crops whose genome was triplicated after divergence from Arabidopsis, we identified B. rapa BAC clones containing these genes by BLAST analysis of B. rapa BAC end sequences against the five corresponding Arabidopsis regions. Subsequent fingerprinting, Southern hybridization, and PCR allowed identification of five BAC clones, one for each of the five circadian-related genes. By draft shotgun sequencing of the BAC clones, we identified the complete gene sequences and cloned the five expressed B. rapa circadian-associated gene members, BrPRRs 1, 3, 5, 7, and 9. Phylogenetic analysis revealed that each BrPRR was orthologous to the corresponding APRR at the sequence level. Northern hybridization revealed that the five genes were transcribed at distinct points in the 24 hour period, and Southern hybridization revealed that they are present in 2, 1, 2, 2, and 1 copies, respectively in the B. rapa genome, which was triplicated and then diploidized during the last 15 million years.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.3
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• pp.199-207
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• 2006

Recent global warming seems to be dramatic and has influenced forest ecosystems. Changes in phonology of biota, species distribution range shift and catastrophic climatic disasters due to recent global warming have been observed during the last century. Korean forests located mainly in the temperate zone also have been experienced climatic change impacts including shifting of leafing and flowering phonology, changes in natural disasters and forest productivity, However, little research has been conducted on the impact of climate change on forest ecosystems in Korea which is essential to assess the impact and extent of adaptation. Also there is a shortage in basic long-term data of forest ecosystem processes. Careful data collection and ecological process modeling should be focused on characteristic Korean forest ecosystems which are largely complex terrain that might have hindered research activities. An integrative ecosystem study which covers forest dynamics, biological diversity, water and carbon flux and cycles in a forest ecosystem and spatial and temporal dynamics modeling is introduced. Global warming effects on Korean forest ecosystems are reviewed. Forestry activity and the importance of forest ecosystems as a dynamic carbon reservoir are discussed. Forest management options and challenges for future research, impact assessment, and preparation of mitigating measures in Korea are proposed.

• Korean Journal of Agricultural and Forest Meteorology
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• v.10 no.1
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• pp.1-8
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• 2008

The concept of growing degree-days (GDD) is widely accepted as a tool to relate plant growth, development, and maturity to temperature. Information on GDD can be used to predict the yield and quality of several crops, flowering date of fruit trees, and insect activity related to agriculture and forestry. When GDD is expressed on a spatial basis, it helps identify the limits of geographical areas suitable for production of various crops and to evaluate areas agriculturally suitable for new or nonnative plants. The national digital climate maps (NDCM, the fine resolution, gridded climate data for climatological normal years) are not provided on a daily basis but on a monthly basis, prohibiting GDD calculation. We applied a widely used GDD estimation method based on monthly data to a part of the NDCM (for Hapcheon County) to produce the spatial GDD data for each month with three different base temperatures (0, 5, and $10^{\circ}C$). Synthetically generated daily temperatures from the NCDM were used to calculate GDD over the same area and the deviations were calculated for each month. The monthly-data based GDD was close to the reference GDD using daily data only for the case of base temperature $0^{\circ}C$. There was a consistent overestimation in GDD with other base temperatures. Hence, we estimated spatial GDD with base temperature $0^{\circ}C$ over the entire nation for the current (1971-2000, observed) and three future (2011-2040, 2041-2070, and 2071-2100, predicted) climatological normal years. Our estimation indicates that the annual GDD in Korea may increase by 38% in 2071-2100 compared with that in 1971-2000.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.15
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• pp.69-75
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• 1974

This experiment was conducted to investigate the influence of different amount of nitrogen and deficiency of soil moisture on yield components of soybean. Soybean were seeded on 1/2000a wagner pot. Deficiency of soil moisture was treated at each growth atage of soybean. 1.In case of deficiencyt of soil moisture at the flowering time in the plot of non-nitrogen(NO D3), the growth duration of soybean was shortened about three to four days. 2. The leaf area was greatly affected by the influence of both treatments till 49days after germinating. 3.The increase of stem height, stem doameter,number of branches and lengeh of the branches came to an end about 70 days after seeding. These growing condition of tje soybean were lowest the plot of No D$_1$,in which the frowth of the soybeans were poor at the early stage. 4.The number of pods was not increased by the increase of fertilizing nitrogenous fertilizer. The number of pods was much decreased by the influence of soil mousture deiciency, and under this condition, the proportion of main stem pods and two or three grain pods was high. 5.The 3rd and 4h nodes and the 10th to 12th nodes from bottom had more pods than the other nodes had, but of the plants had grown well, they had more pods on the 3rd and 4th nodes, but if the plants had grown poorly, they had more pods on the 10th to 12th nodes. 6.The content of protein in the soybean was low at the plit of N。D$_4$which had not heavy weight of 100 grains, and the content of oil in the soybean was low in the plot in which each plant had a small number of grains.