• Journal of Bio-Environment Control
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• v.17 no.2
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• pp.132-137
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• 2008

This experiment was conducted to investigate the effect of supplemental lighting of low light intensities on growth and yield of rose 'Vital' in a forcing culture. Metal halide lamp (MH), High pressure sodium lamp (HSP), and MH+HSP were used as the light sources, and they were set up at a $310\;cm{\times}450\;cm$ interval and at 120cm above the culture beds. Light intensity at 1m point distance from supplemental lighting sources was $32{\sim}34\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Days to the 1st and 2nd harvests decreased by $5{\sim}8$ and $3{\sim}5$days, respectively in supplemental lighting treatment as compared to the control. Days to harvesting was the shortest in MH+HPS treatment, followed by BPS and MH, although there was no significant difference between HPS and MH treatments. The growth was better and incidence of blind shooting decreased by $5{\sim}7%$ in supplemental lighting treatments than the control, increasing marketable cut flowers. The incidence of blind shoot was the lowest in MH+BPS treatment, and there were no significant difference between MH and BPS treatments. In conclusion, supplemental lighting of low light intensities was effective in reducing days to flowering and reduced occurrence of blind shoots.

• Journal of Bio-Environment Control
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• v.25 no.4
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• pp.294-301
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• 2016

This study was conducted to analyze the seedling quality of korean melon and the growth after transplanting of korean melon nursed under the LED sources. LED sources were RB7 (Red:Blue=14:2), RB3 (Red:Blue=12:4) and Blue(B=16). Photosynthetic photon flux density(PPFD) was 50, 100 and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The lighting treatment was started after graft-taken and was applied for 20 days at 4 hours(05:30 and 07:30, 17:30 and 19:30) per day. Plant height and stem diameter of scion were longer and thicker under a high ratio of blue light condition. Dry matter ratio and compactness were highest in RB3 compared to the other LED sources treatments. $CO_2$ exchange rate increased $5.44{\mu}molCO_2{\cdot}m^{-2}{\cdot}s^{-1}$ under RB7 $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and dropped to negative values under control. PPFD $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ of RB3 resulted in the longest plant height by 132.3cm and flowering ratio also was the highest by 75%.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.180-185
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• 2015

Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.23-29
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• 2009

We have previously isolated a CCCH type zinc-finger protein gene, OsZF2 (Oryza sativa Zinc Finger 2), from the cold-treated rice cDNA library. To investigate the potential role of OsZF2, transgenic rice lines over-expressing OsZF2 under the control of CaMV 35S promoter have been developed through Agrobacterium-mediated transformation. Elevated level of OsZF2 transcripts was confirmed by RNA gel blot analysis in transgenic rice. Under the 100 mM NaCl condition, the transgenic rice showed significantly enhanced growth rate in terms of shoot length and fresh weight, implicating that OsZF2 is likely to be involved in salt response of rice. In the field condition, however, the transgenic rice showed a dwarf phenotype and flowering time was delayed. Genome expression profiling analysis of transgenic plants using the 20K NSF rice oligonucleotide array revealed many up-regulated genes related to stress responses and signaling pathways such as chaperone protein dnaJ 72, salt stress-induced protein, PR protein, disease resistance proteins RPM1 and Cf2/Cf5 disease resistance protein, carbohydrate/ sugar transporter, OsWAK kinase, brassinosteroid LRR receptor kinase, and jasmonate O-methyltransferase. These data suggest that the CCCH type zinc-finger protein OsZF2 is a upstream transcriptional factor regulating growth and stress responsiveness of rice.

• Molecules and Cells
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• v.38 no.3
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• pp.259-266
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• 2015

The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.

• Research in Plant Disease
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• v.20 no.2
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• pp.79-86
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• 2014

In order to seek more eco-friend, economic and safer quarantine method than current methyl bromide fumigation, the convergent treatment with 200 Gy of gamma irradiation and several chemicals such as nano-siver particles (NSS), sodium dichloroisocyanurate (NaDCC) was tried on the cuttings of lily in the packing of catonnage box for export. With 6 independent experiments of gamma irradiation on the three lily cultivars, cvs. Siberia, Le reve and Sorbonne, incidence and severity of lily leaf blight was investigated on leaves and petals at 8-d after infection. 200 Gy of gamma irradiation decreased at 13-25% of severity on the leaf of Sorbonne, but it increased at 2-5% of severity on the leaf of Siberia and Le reve. Chemical substitutes such as NSS and NaDCC were not effective to control of lily blight on cuttings. By 200 Gy of gamma irradiation treatment, chlorophyll contents were statistically significantly decreased at 12-d after irradiation and the longevities vaselife of fully open flower of Siberia and Sorbonne were increased at 0.4 to 1.2 days. In addition, the relative fresh weights of the gamma irradiated cuttings were severely dried compared to the non-irradiated control. On the other hands, the symptoms of phyto-toxicity of high dose gamma irradiation at 1 or 2 kGy on cv. Siberia were to be blight at the tip of bloom, bent necks of flower, and delayed the process of flowering.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.85-93
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• 1984

This research was carried out to study the seed dormancy of radishes which were used for summer vegetation. The differences of dormancy of cultivars, maternal effects, the effect of full day length to mother plants, and the effects of chemical treatments for breaking the seed dormancy were considered for this study. These results sere summarized as follows; 1. The germination percentage of 7 cultlvars sown just after harvest were as follows;SP70-:0, PS11-:37.5, GJ75-:75.5, JD127-:77.5, YH85-:81.0, M55-:96.5, and JC73-:98.0%. 2. The dormany of $F_1$ seed of radish was severe when SP70-was used for mother plant. 3. Germination behavior was different by the maturity of plants grown for seed production. The dormancy of seed harvested from immature field-grown plants sown Nov. 10 was deeper than mature plants sown Mar. 22. 4. The germination ratio of tire seed of SP70-radish was increased by treatment of full day length to tire mother plant during full period of growing or since first flowering stage, while that was 0% by treatment since yellow ripening stage. 5. GA 200 ppm+thiourea 0.2% treatment was most effective for breaking of radish seed dormancy.

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.77-84
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• 1984

This experiment was carried out to determine the effects of night temperature and amount of fertilization on growth and photosynthesis of red pepper plants for providing some basic imformation needed in improving the productivity of red pepper. 1. Plant height, stem length, number of flower buds, dry weight of plants and photosynthetic rate were higher in King Gun Gochu than in Dohusa, but number of leaves, number of internodes and leaf area we re higher in Dohusa compared to King Gun Gochu. 2. Plant height, number of leaves, number of flower buds, dry weight of plants, leaf area and photosynthetic rate were significantly increased by 50% increased fertilization compared to normal fertilization. 3. High night temperature($25^{\circ}C$) treatment increased plant height, stem length, number of leaves, number of internodes, number of flower buds, dry weight of plants and leaf area. 4. RGR and LAR were increased by 50% increased fertilization and high night temperature but NAR was decreased by high flower buds 5. Photosynthetic rate of King Gun Gochu was increased by 50% increased fertilization and high light intensity. 6. Number of flower buds was increased at King Gun Gochu by 50% increased fertilization and high night temperature ($25^{\circ}C$).

• Korean Journal of Agricultural Science
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• v.9 no.1
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• pp.231-238
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• 1982

The study was done to determine the proper transplanting dates and seedling ages of rice when mechanical transplanting was attempted. Heading dates of the Milyang #23, developed from Tong-il rice was delayed by delayed transplanting and the percent of filling grain was also low. The proper heading dates for the variety should be the first part of August and the transplanting should be done by the end of May. The presumed last days for the transplantation was considered early part of June. 30 to 40 days old seedlings were very effective to have healthy rooting systems. However, the Milyang #15 had shown more tolerance to the late transplantation than Milyang #23 and the degree of delaying heading dates due to the delayed transplantation was rather smaller than that of the Milyang #23. Even when the Milyang #15 was transplanted on June 30, the heading dates were around the middle of August and the ripening percentage was comparatively high and yield was also high. Therefore, the proper heading dates of the Milyang #15 might be around the middle of August and transplanting dates should be the first part of June. The possible last dates of transplantation of the Milyang #15 was assumed to be the last days of June and the proper seedling ages would be 20 to 30 day after sowing.

• Korean Journal of Agricultural Science
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• v.33 no.2
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• pp.141-147
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• 2006

This study was conducted on a commerce silt loam soil at paddy field in the southwestern Korea ($36^{\circ}N$ lat). In the study of planting time, seed were hand planted at 24 May, 14 June and 5 July, and at 24 May, 14 June and 5 July in 2005, respectively. Two seedlings plants per hill were taken prior to V3 stage. Fertilizer was applied prior to plant at a rate of 3.0 - 3.0 - 3.4 g (N - P - K) per square meter. Experimental design was a randomized complete block in a split plot arrangement with three replications. Yield was significantly affected by different of the planting times and cultivars. The seed yield in planting time was the highest on late in May 24 but was the lowest on July 5. Also, the plant time significantly affected on increasing pod and seed number and seed weight. The days from emergence to flowering and maturity was reducing tendency with late planting time. The highest and lowest total dry matter production per square meter appeared at late in May and early in July with planting time, respectively. RGR, LAR and SLA was increased with late planting time. Photosynthetic rate at each planting time was not significant on the expanded the highest leaf position but at seed development stage, it was higher photosynthetic rate at May 24 than that of late planting time on $7^{th}$ laef position from the basal part.