• 제목/요약/키워드: FADS2

검색결과 20건 처리시간 0.022초

Comparison of overfed Xupu and Landes geese in performance, fatty acid composition, enzymes and gene expression related to lipid metabolism

  • Liu, Xu;Li, Peng;He, Changqing;Qu, Xiangyong;Guo, Songchang
    • Asian-Australasian Journal of Animal Sciences
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    • 제33권12호
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    • pp.1957-1964
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    • 2020
  • Objective: The aim of this study was to compare overfeeding performance, fatty acid composition, blood chemistry, enzymes and genes expression overfed Xupu and Landes geese. Methods: Sixty male Xupu geese (80 d) and Landes geese (80 d) were selected. After a period of one-week of pre-overfeeding, Xupu and Landes geese were overfed three meals of 550 and 350 g/d, respectively, of a high-carbohydrate diet in the first week of the overfeeding period. The next week, geese were given four meals of 1,200 and 850 g/d, respectively, over 8 to 14 d. Finally, geese were given five meals of 1,600 and 1,350 g/d, respectively, for the last two weeks. Results: After overfeeding for 28 d: Compared with Landes geese, Xupu geese liver weight and liver-to-body weight ratio decreased (p<0.05), while final weight, slaughter weight, total weight gain, abdominal fat weight, and feed-to-liver weight ratio increased (p<0.05). The levels of elaidic acid (C18:1t9), oleic acid (C18:1n-9), eicosenoic acid, and arachidonic acid in the liver of Xupu geese significantly increased (p<0.05), and the levels of myristic acid and stearic acid significantly decreased (p<0.05), while methyleicosanoate acid significantly increased (p<0.05). Xupu geese had higher plasma concentrations of triglyceride and very low density lipoprotein cholesterol (p<0.05), and decreased activities of alanine aminotransferase, aspartate aminotransferase, and lipase (LPS) (p<0.05). Landes geese had higher LPS activity (p<0.05), but lower cholinesterase activity (p<0.05) when compared with Xupu geese. The mRNA expression levels of fatty acid dehydrogenase (FADS) gene, elongase of long-chain fatty acid 1 (ELOVL1) gene, ELOVL5, and acyl-Co A: cholesterol acyltransferase 2 (ACAT2) gene were significantly upregulated (p<0.05) in Landes goose when compared with Xupu geese. Conclusion: This study demonstrates that the liver production performance of Landes geese was better than that of Xupu geese to some extent, which may be closely related to LPS activity, as well as the expression of FADS, ELOVL1, ELOVL5, and ACAT2.

한우 등심조직 내 인슐린 조절 유전자의 발현이 도체중에 미치는 영향에 관한 연구 (Association of Insulin-related Genes Expression with Carcass Weight in Loin Muscle of Korean Cattle (Hanwoo))

  • 임다정;조용민;채한화;이승환;최봉환;김남국
    • 생명과학회지
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    • 제25권1호
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    • pp.8-15
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    • 2015
  • PPAR signaling pathway는 지방대사와 지방세포 분화를 조절하는 대표적인 대사회로이기 때문에 가축에 있어서 주로 육질과의 연관성 연구가 진행되었다. 하지만, 최근 들어 육량(체중)과 관련이 있다는 연구결과가 보고되고 있다. 본 논문에서는 PPAR signaling pathway에 존재하는 48개 유전자 중에서, pathway 분석을 통하여 체중에 가장 영향을 주는 인슐린 대사 호르몬에 의해 조절 받는 16개 유전자를 선별하여 거세 한우 20두에서 유전자 발현을 조사하였다. 유전자 발현과 도체중과의 관련성 분석을 위하여 회귀분석을 수행하였으며, 3개 유전자(ACSL6, FADS2, ILK)가 통계적으로 유의한 결과(p<0.05)를 보였다. 마지막으로, pathway 분석을 통하여 한우의 도체중과 관련이 있는 3개 유전자를 공통적으로 조절하는 포도당(D-glucose)이 존재함을 확인하였다.

K 패션 착용경험에 관한 연구 -20~30대 네팔, 베트남, 몽골 외국인을 중심으로- (A Study on Foreigners' Experience ofWearingK Fashion -Focusing on Nepalese, Vietnamese, and Mongolian Foreigners in 20s and 30s-)

  • 머허러전 시르저나;차수정
    • 패션비즈니스
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    • 제28권2호
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    • pp.15-32
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    • 2024
  • This study aimed to investigate the perceptions and preferences of foreigners residing in Korea towards K-fashion. It sought to provide suggestions for the future development of K-fashion targeting this growing demographic. The results revealed that the longer foreigners had lived in Korea, the more positively they rated Korean fashion, shopping, and culture. The majority of foreigners purchased K-fashion items online, with popular choices including T-shirts, jackets, shirts, and jeans. Both men and women identified shoulders as a common problematic area when wearing clothing, with some issues also reported in the lumbar region. In terms of preferences, younger males (teens and twenties) were more inclined towards fads and celebrity wear compared to older males (thirties and forties). Similarly, younger females (teens and twenties) showed a higher preference for functionality and celebrity wear compared to older females (thirties and forties). These findings suggest that men tend to prioritize aesthetic factors when choosing K-fashion, while women prioritize practical considerations. To better cater to foreign consumers, suggestions for the development of K-fashion include promoting it through K-pop stars, considering dimensions related to foreigners' shoulders when designing top items, diversifying colors, sizes, and patterns, and providing English versions of online shopping platforms.

몽골 나담축제 복식에 관한 연구 (A Study on the Costumes of the Mongol Naadam Festival)

  • 홍정민
    • 복식
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    • 제52권7호
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    • pp.167-177
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    • 2002
  • This study revolves around the costume of the Naadam festival worn in the opening ceremony. during competition and the costume of the public. their purpose are two fold. First they are to be the main reference for the diverse Mongolian costumes that have been researched. Secondly, they will help us understand better the culture that revolves around Mongolian costumes. The results are as follows : 1) The opening ceremony costume, The opening ceremony starts with a procession of soldiers with 9 flags. The flags are made from the hairs of white horses and they signify peace and tranquility, ever since the beginning of Chinggis Khans time. The costumes resemble a European military uniform 2) The costumes for competition. $\circled1$ Wrestlers' costume comprises of a jacket with sleeves but no front and shorty shorts of red or blue colored silk, a traditional hat and traditional boots. $\circled2$ Archers' costume comprises of their traditional costume, deel, hat and boots. $\circled3$ Horse riders who are usually children wear a comfortable and simple jacket and pants. 3) The costume of the public : The general public that attended the festival were dressed in a western fashion but differ according to different age groups $\circled1$ The costumes of the younger Mongolian : The majority of the men wore traditional costumes that were appropriate to the festival, the women did not as they were more prone to the influence of fads of western cultures and dressed in a more daring fashion. In addition. the children also dressed in a more modem western fashion. $\circled2$ The costumes of the elderly Mongolian : The elderly, oblivious of whatever season it was, were dressed in a more traditional fashion which is the deel.

코스닥시장 IPO의 초과수익률 원인 분석

  • 박수웅;이명철;남기풍
    • 재무관리논총
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    • 제8권1호
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    • pp.157-188
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    • 2002
  • 본 연구는 우리나라 IPO시장에서 나타나고 있는 초과수익률의 원인을 밝히고자 최근 벤처금융의 중심으로 급부상하고 있는 KOSDAQ시장의 공모 IPO를 대상으로 실증분석을 시도하였다. 연구결과 상장일 표본전체 IPO의 초과수익률(AR1)이 9.91%로 나타났으며, 벤처캐피탈투자 IPO는 5.13%, 비투자 IPO는 13.29%로 나타났다. 상한가의 행진이 종료된 날을 기준으로 한 표본전체 IPO의 초과수익률(AR2)은 30.97%, 벤처캐피탈 투자기업 IPO는 24.34%, 비투자기업 IPO는 34.67%로 나타났다. 초과수익률의 원인을 분석하기 위한 8개의 변수 중 IPO의 수요증감 척도인 KOSDAQ 지수는 초과수익률(AR)과는 양(+)의 관계로, 벤처캐피탈리스트들의 능력 및 가치책정의 행태를 엿볼 수 있는 변수인 본질가치와 공모가액의 차이는 음(-)의 관계로, 사업규모나 자본조달의 크기를 엿볼 수 있는 공모금액의 크기는 양(+)의 관계로 1% 유의수준에서 유의한 것으로 나타났다. 그러나 여타 변수들은 통계적으로 유의성을 확보하지는 못하였으나 초과수익률의 원인이론으로 '신호이론'과 '투기적 거품가설'에 의한 설명이 가능하였으며 분석결과를 다음과 같이 정리할 수 있었다. 첫째, IPO 본질가치에 대한 신뢰와 IPO의 발행규모에 대한 신뢰수준이 통계적으로 유의한 결과로 나타났는바 이러한 결과는 기업가치와 벤처 또는 사업의 계속적 수행 등에 대한 신호역할을 수행하고 있다고 할 수 있으며 상장초기 초과수익률은 주로 이러한 신호역할에 의한 수요집중으로 발생된 결과인 것으로 사료되었다. 둘째, 노련한 벤처캐피탈회사로 선정된 KTB의 투자기업들은 상장 후 4주간의 거래에 있어 AR 평균이 음(-)으로 여타 벤처캐피탈이 투자된 기업보다 손실 폭이 더욱 켰음에도 불구하고 상장일 초과수익률 AR2가 매우 높게 나타나 우리나라 IPO시장에 있어 과민반응(fads) 현상이 존재하고 있는 것으로 분석되었다. 셋째, 벤처캐피탈 투자 IPO의 초과수익률이 상대적으로 낮아 벤처캐피탈 투자여부가 IPO의 저가발행 수준을 축소하고 있어 벤처캐피탈리스트의 제 3자 보증역할이 어느 정도는 수행되고 있는 것으로 분석되었다.

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Usability Evaluation of Mobile Banking Applications in Digital Business as Emerging Economy

  • Hamid, Khalid;Iqbal, Muhammad Waseem;Muhammad, Hafiz Abdul Basit;Fuzail, Zubair;Ghafoor, Zahid Tabassum;Ahmad, Sana
    • International Journal of Computer Science & Network Security
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    • 제22권2호
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    • pp.250-260
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    • 2022
  • Mobile Banking Applications (MBAPs) is one of the recent fads in mobile trading applications (Apps). MBAPs permit users to execute exchanges of money and many more whenever it might suit them; however, the primary issue for mobile banking Apps is usability. Hardly any investigation analyzes usability issues dependent on user's age, gender, exchanging accomplices, or experience. The purpose of this study is to determine the degree of usability issues, and experience of mobile banking users. The survey employs a quantitative method and performs user experiment on 240 participants with six different tasks on the application's interface. The post experiment survey is done with concerning participants. On the other hand, banking experts and Information Technology (IT) expert's group is also involved after the experiment. Expert's opinions about existing mobile banking Apps and suggestions for improving usability of MBAPs are collected through physical means (like questionnaire and interview) and online means like Google form. After that comparison of the opinions of users and experts about MBAPs is performed. The experimentation measures the tasks usability of various mobile banking apps with respect to its effectiveness, efficiency, trustfulness, learnability, memorability and satisfaction. The usability testing was led at different Universities and the outcomes acquired show that there are privacy and trust issues with their mobile banking apps. There is also a gap between users and experts which should be minimized by applying customized usability models, modes concept like other application software and also by adding complete features of banking in MBAPs. It will benefit mobile banking apps users, developers and usability engineers by providing user-friendly which are up to the mark of user's requirements.

Phosphoprotein Enriched in Astrocytes 15 (PEA15)가 과발현하는 형질전환 돼지의 생산 (Production of a Transgenic Enriched in Pig Overexpressing Phosphoprotein Astrocytes 15 (PEA 15))

  • 이휘철;김현미;이승훈;오건봉;정학재;양병철;김경운;이풍연;박진기;장원경
    • Reproductive and Developmental Biology
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    • 제35권3호
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    • pp.239-245
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    • 2011
  • The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These fads suggest that expression vector system is normally expressed in the transgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

Study on the fatty acid profile of phospholipid and neutral lipid in Hanwoo beef and their relationship to genetic variation

  • Beak, Seok-Hyeon;Lee, Yoonseok;Lee, Eun Bi;Kim, Kyoung Hoon;Kim, Jong Geun;Bok, Jin Duck;Kang, Sang-Kee
    • Journal of Animal Science and Technology
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    • 제61권2호
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    • pp.69-76
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    • 2019
  • Maize which has very high omega-6 fatty acid content has been used as a main feed grain for Hanwoo beef production to increase marbling, and thus omega-6 to omega-3 fatty acids ratio in Hanwoo beef is expected to be biased. To elucidate the current status of omega fatty acids ratio in Hanwoo beef, fatty acid profiles of neutral lipid and phospholipid fraction were analyzed separately using 55 Hanwoo steers' longissimus dorsi muscle slaughtered at Pyeongchang, Korea from Oct. to Nov. 2015. In addition, an association study was conducted to evaluate associations between single nucleotide polymorphism (SNP) markers from references and omega fatty acid profiles in phospholipid of Hanwoo beef samples using analysis of variance (ANOVA). In neutral lipid fraction, composition of saturated and monounsaturated fatty acids was higher and polyunsaturated fatty acids was lower compared to those in phospholipid fraction. The mean n-6/n-3 ratios of Hanwoo were $56.059{\pm}16.180$ and $26.811{\pm}6.668$ in phospholipid and neutral lipid, respectively. There were three SNPs showing statistically significant associations with omega fatty acid content. GA type of rs41919985 in fatty acid synthase (FASN) was significantly associated with the highest amount of C20:5 n-3 (p = 0.031). CC type of rs41729173 in fatty acid-binding protein 4 (FABP4) was significantly associated with the lowest amount of C22:2n-6 (p = 0.047). AG type of rs42187261 in FADS1 was significantly linked to the lowest concentration of C20:4 n-6 (p = 0.044). The total n-6/n-3 ratio of the steer which has all four SNP types in above loci (27.905) was much lower than the mean value of the total n-6/n-3 ratio in phospholipid of the 55 Hanwoo steers ($56.059{\pm}16.180$). It was found that phospholipid and neutral lipid of Hanwoo have very high n-6/n-3 ratios compared to the reported data from different cow breeds. Four SNPs in genes related with fatty acid metabolism showed significant associations with the fatty acid profile of phospholipid and may have potential as SNP markers to select Hanwoo steers in terms of n-6/n-3 balance in the future.

낙관적 투자자의 기대가 핫마켓상황 IPO 시장의 이상현상에 미치는 영향력 검증 (The Effect of Optimistic Investors' Sentiment on Anomalious Behaviors in the Hot Market IPOs)

  • 김현아;정성창
    • 재무관리연구
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    • 제27권2호
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    • pp.1-33
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    • 2010
  • 본 연구는 IPO 시장에서 나타나는 높은 초기수익률 및 장기저성과 현상이 주로 핫마켓상황에서 신규공개한 기업에 의해 야기되는지 분석하고, 이러한 핫마켓상황 공모 시장의 이상현상을 낙관적 투자자의 기대라는 행태적 관점에서 분석하였다. 특히, 낙관적 투자자의 기대가 영향을 미치는 가격결정메커니즘 측면과 낙관적 투자자의 기대를 이용하고자 하는 기업의 기회주의적 행동 측면으로 구분하여 살펴보았다. 국내 선행연구는 이상현상에 대한 원인을 버블로 해석할 뿐 이에 대한 체계적 실증연구가 미흡하다. 본 연구는 2001년부터 2005년 동안 코스닥시장에 신규상장을 마친 432개 기업들을 대상으로 하였으며, 이들 표본을 시장상황에 따라 '핫마켓 IPO'와 '콜드마켓 IPO'로 구분하여 비교 분석하였다. 분석결과 전체 IPO 시장의 이상현상은 대부분 '핫마켓 IPO'에 의한 것으로 생각되었다. 그리고 핫마켓에 존재하는 낙관적 투자자의 기대는 초기수익률을 높이지만 장기성과에는 부(-)의 영향을 미침으로써 핫마켓상황의 낙관적 투자자의 기대가 공모 시장의 이상현상을 초래하는 것으로 나타났다. 마지막으로, 코스닥시장의 핫마켓상황은 질적 여건이 좋지 않은 기업들에게 단지 기회로 이 용된다는 '기회의 창' 가설을 지지하는 증거는 발견되지 않았다.

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붉은 지렁이(Lumbricus rubellus) 체내로부터 정제한 Phenoloxidase (Endogenous Phenoloxidase Purified from an Earthworm, Lumbricus rubellus)

  • 백승렬;조은정;유경희;김유삼;서정진;장정순
    • 한국동물학회지
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    • 제39권1호
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    • pp.36-46
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    • 1996
  • 붉은 지렁이(Lumbricus rubellus)로부터 체내에 존재하는 phenoloxidase (EPO)를 ammonium sulfate. Blue-2, Phenyl-, Q-sepharose chromatography등을 이용하여 정제하였다. 이 효소는 SDS-PAGE상에서 59 kDa의 분자량을 갖는 단일 단백질로 나타났으며 nondenatudng-PAGE를 이용하여 DL-dopa를 기질로 in situ 염색 결과, 210 kDa 보다 다소 큰 단일 band가 dopachrome 침착에 의해 형성되었다. 이는 곧 이 효소가 자연상태에서 복합체의 형태로 존재하고 있음을 의미한다. 또한 이 효소는 monophenolase 활성도, 즉 tyrosine을 dopa로 전환시키는 활성도도 갖고 있음을 470nm에서 dopachrome축적을 관찰함으로써 확인할 수 있었다. Phenyithiourea(PUT), 1, 10-phenanthroline, EDTA, EGTA등을 사용한 효소억제 실험 결과, PTU만이 65 $\mu$M의 IC 0.5로 효소 활성도를 효과적으로 억제시켰다. 이는 EPO의 촉매기작에서 구리가 매우 중요한 역할을 하고 있음을 의미한다. 이 효소는 L-dopa를 기질로 사용하였을 때 35$^{\circ}C$와 pH8.0에서 최적의 활성도를 나타내었다. EPO의 L-dopa에 대한 Km은 pH6.5와 8.0에서 각각 1.86 mM과 13.8 mM로 나타났다. 또한, pH 8.0에서 Vmax는 pH 6.5에서 보다 약 6.6배 높은 반면, 각 조건에서 촉매 효율성은 거의 차이가 없음 [(kat/Km)pH8.0/(kcat/Km)pH6.5 = O.92]을 알 수 있었다. 따라서, 이 사실은 EPO 촉매기작에 미치는 pH의 효과가 효소 자체에보다는 기질 또는 효소-기질 복합체 형성과정에 영향을 줌을 의미한다. 이와 같은 사실을 종합해 보면, L. rubellus에 존재하는 phenoloxidase는 oligomeric form을 가지며 활성화 되기 위한 제한적 단백질 가수분해를 필요로 하지 않는다. 따라서, prophenoloxidase activating system의 존재 가능성을 완전히 배재할 수는 없으나 지렁이 체내의 PO는 최소한 부분적으로나마 latent from으로 존재함을 확인할 수 있었다. 이는 외부 침입시 host를 보호하기 위한 방법으로 EPO를 latent from으로 유지 시킬 수 있는 또는 활성화 시킬 수 있는 조절기작의 존재를 예측하게 한다.

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