• Title/Summary/Keyword: F2 population

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Genetic Structure of the Mulberry Silkworm Population in Sri Lanka: I. Estimation of Combining Ability and Heritability

  • Lea, Ho-Zoo;Alwis, Siriani-M.de
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.10-15
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    • 1995
  • Genetic characterization of Sri Lankan silkworm bivoltine population has not been at-tempted so far, since its sporadic introduction of bivoltine strains into the island, starting from the 1950's. Genetic structure of Sri Lankan population of mulberry silkworm Bombyx mori was investigated through estimation of general (GCA) and specific combining ability(SCA) and heritability(${h^2}_B$), on the economic quantitative characters from leading 8 inbreds and their 28 F1's in a half diallel cross, in an attempt to utilize the estimates in determination of future breeding methods and to predict the breeding value over the phenotypic value. It was found that the breeding population of the bivoltine silkworm in Sri Lanka has still maintained considerable amounts of additive gene action as well as nonadditive. For some time in the future, both breeding strategies of "selection without inbreeding" and also "inbreeding followed by crossing" should therefore be effective in genetic improvement of economic characters investigated. In addition, superior combiners in general and in specific F1′s were identified for each of 6 economic characters, to be immediately utilized in selection and also in cross breeding programs in Sri Lanka.

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Genetic Diversity and Population Structure of Kaloula borealis (Anura, Microhylidae) in Korea

  • Yang, Suh-Yung;Kim, Jong-Bum;Min, Mi-Sook;Suh, Jae-Hwa;Kang, Young-Jin
    • Animal cells and systems
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    • v.4 no.1
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    • pp.39-44
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    • 2000
  • To assess the genetic diversity and population structure of Korean K. borealis, allozyme analysis was performed. The average genetic variability of Korean K. borealis populations was %P=13.2, Ho=0.048, and He=0.045. This value was the lowest in comparison with other Korean amphibian species studied. Also, the value was much lower than that of a reference population from Chinese K. borealis (%P=50, Ho=0.125, He=0.172). Wright's F-statistics showed that Korean K. borealis has distinctly low level of gene flow among regional populations (F$_{ST}$=0.339, Nm=0.487) in comparison with other Korean amphibian species studied. However, the average level of genetic divergence among Korean K. borealis populations was moderate (Nei's D=0.020). Therefore, it appeared that low levels of genetic diversity (He=0.045) and gene flow (Nm=0.487) among regional populations ave probably due to the results of decreasing population size and patchy distribution of this species in Korea.

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Inheritance of Insecticide Resistance to Plant- and Leaf-hoppers Inherited Properties of MEP Resistance to Small Brown Plant-hopper (Laodelphax striatellus Fallen) (멸구 매미충류에 대한 약제저항성의 유전성에 관한 연구 I. 애멸구의 MEP제에 대한 약제저항성의 유전적 특성)

  • Shim Jai Wook
    • Korean journal of applied entomology
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    • v.17 no.2 s.35
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    • pp.75-80
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    • 1978
  • The study was conducted to determine the inherited properties of the MEB resistance to natural population of the small brown plant-hopper(Laodelphax striatelluss). The plant-hoppers were collected from the natural population of Naju area where the highest insecticide resistance of MEF to the green rice leaf hopper (Nephotettix cincticeps) have been examined in 1976. And Naju collections were crossed to the susceptible Lab stock to examine the MEP resistance in the $F_1,\;BC_1F_1\;and\;F_2$ and $F_2$ populations. Ail the data were analyzed by the probit method. There was a difference in MEP resistance between Naju collection and susceptible Lab stock, showing $LD_{50}$ value of the former was 0.0029ug/insect compared to 0.0008ug/insect for the later. The $LD_{50}$ values and dosage-mortality lines of the $F_1$ and $BC_1F_1$ tended to close their resistant parent. and it was considered that the character of the MEP resistance in the Naju collection of the small brown plant hopper was controlled by the genetic traits. However, $LD_{50}$ value and dosasage-mortality lines of the $F_2$ populations were intermediate to their parents, it would be conclusive that the trait will be governed by a interaction of the genes or factors rather than the single genic control.

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ON THE MARTINGALE PROBLEM AND SYMMETRIC DIFFUSION IN POPULATION GENETICS

  • Choi, Won;Joung, Yoo-Jung
    • Journal of applied mathematics & informatics
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    • v.28 no.3_4
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    • pp.1003-1008
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    • 2010
  • In allelic model $X\;=\;(x_1,\;x_2,\;\cdots,\;x_d)$, $$M_f(t)\;=\;f(p(t))\;-\;\int_0^t\;Lf(p(t))ds$$ is a P-martingale for diffusion operator L under the certain conditions. In this note, we define $T_tf\;=\;E_{p_0}^{p^*}\;[f((P(t))]$ for $t\;{\geq}\;0$ for using a new diffusion operator $L^*$ and we show the diffusion relations between $T_t$ and diffusion operator $L^*$.

Linkage Map Construction and Molecular Genetic Approach in Capsicum spp.

  • Kim, Byung-Dong
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.5
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    • pp.367-373
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    • 2000
  • We have constructed a molecular linkage map of chili pepper (Capsicum spp.) in an interspecific (C. annuum cv. TF68 x C. chinense cv. Habanero) F$_2$ population of 107 plants with 150 RFLP and 430 AFLP markers. The resulting linkage map consists of 11 large (206-60.3 cM) and 5 small (32.6- 10.3 cM) linkage groups cover-ing 1,320 cM with an average map distance between framework markers of 7.5 cM. Most (80%) of the RFLP markers were pepper-derived clones and these markers were evenly distributed across the genome. By using 30 primer combinations, 444 AFLP markers were generated in the F$_2$population. The majority of the AFLP markers clustered in each linkage group, although PstI/MseI markers were more evenly distributed than Eco RI/MseI markers within the linkage groups. Genes for biosynthesis of carotenoids and capsaicinoids were mapped on our linkage map. This map will provide the basis of studying secondary metabolites in pepper.

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THE APPLICATION OF STOCHASTIC ANALYSIS TO POPULATION GENETICS MODEL

  • Choi, Won
    • Journal of applied mathematics & informatics
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    • v.23 no.1_2
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    • pp.455-460
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    • 2007
  • In allelic model $X=(x_1,\;x_2,\;{\cdots},\;x_d)$, $$M_f(t)=f(p(t))-{\int}_0^t\;Lf(p(t))ds$$ is a P-martingale for diffusion operator L under the certain conditions. In this note, we try to apply diffusion processes for countable-allelic model in population genetic model and we can define a new diffusion operator $L^*$. Since the martingale problem for this operator $L^*$ is related to diffusion processes, we can define a integral which is combined with operator $L^*$ and a bilinar form $<{\cdot},{\cdot}>$. We can find properties for this integral using maximum principle.

Evaluation of Reciprocal Cross Design on Detection and Characterization of Non-Mendelian QTL in $F_2$ Outbred Populations: I. Parent-of-origin Effect

  • Lee, Yun-Mi;Lee, Ji-Hong;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.12
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    • pp.1805-1811
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    • 2007
  • A simulation study was conducted to evaluate the effect of reciprocal cross on the detection and characterization of parent-of-origin (POE) QTL in $F_2$ QTL populations. Data were simulated under two different mating designs. In the one-way cross design, six $F_0$ grand sires of one breed and 30 $F_0$ grand dams of another breed generated 10 $F_1$ offspring per dam. Sixteen $F_1$ sires and 64 $F_1$ dams were randomly chosen to produce a total of 640 $F_2$ offspring. In the reciprocal design, three $F_0$ grand sires of A breed and 15 $F_0$ grand dams of B breed were mated to generate 10 $F_1$ offspring per dam. Eight $F_1$ sires and 32 $F_1$ dams were randomly chosen to produce 10 $F_2$ offspring per $F_1$ dam, totaling 320 $F_2$ offspring. Another mating set comprised three $F_0$ grand sires of B breed and 15 $F_0$ grand dams of A breed to produce the same number of $F_1$ and $F_2$ offspring. A chromosome of 100 cM was simulated with large, medium or small QTL with fixed or different allele frequencies in parental breeds. A series of tests between Mendelian and POE models were applied to characterize QTL as Mendelian, paternal, maternal or partial expression QTL. The overall detection powers were similar between the two mating designs. However, the proportions of paternally expressed QTL that were declared as paternal QTL type were greater in the reciprocal cross design than in the one-way cross, and vice versa for Mendelian QTL. When QTL alleles were segregating in parental breeds, a significant proportion of Mendelian QTL were spuriously declared POE QTL, suggesting that care must be taken to characterize imprinting QTL in a QTL mapping population with a small number of $F_1$ parents.

Population Genetic Analyses of Gibberella fujikuroi Isolated from maize in Korea

  • Park, Sook-Young;Seo, Jeong-Ah;Lee, Yin-Won;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.17 no.5
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    • pp.281-289
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    • 2001
  • We analyzed 88 strains of Gibberella fujikuroi (Analmorph: Fusarium section Liseola) from maize in Korea for mating population, mating type, fumonisin production vegetative compatibility, and random amplified polymorphic DNA (RAPD) patterns. We found 50 strains that were MATA-2, 22 that were MATA-1, 1 that was MATD-1, and 15 that were not reproducibly fertile with any of the mating type testers. Of the 50 MATA-2, 15 were female fertile, while 10 of the 22 MATA-1 strains were female fertile. A total of 1,138 nitrate non-utilizing (nit) mutants were recovered from a total of 88 strains. These strains were grouped into 39 vegetative compatability groups (VCGs) by demonstrating heterokaryosis between nit mutants. A single maize ear could be infected by more than one VCG of F. moniliforme. RAPD analysis measured genetic diversity among 63 strains of F. moniliforme. Several VCGs were distinguished by RAPD fingerprinting patterns. Most strains produced significant levels of fumonisins. However, 6 MATA-2 strains from a single VCG produced higher levels of fumonisin $\textrm{B}_3$ than that of fumonisin $\textrm{B}_1$ or $\textrm{B}_2$. From these data, we concluded that most Korean strains of F. moniliforme associated with maize belonged to mating population A and produced significant levels of fumonisins. Futhermore, RAPD analysis could differentiate strains associated with different VCGs.

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MORPHOMETRIC ANALYSIS OF PLECOGLOSSUS ALTIVELIS OF CHEJU ISLAND AND THE NAKDONG RIVER (제주도 및 낙동강산 은어군의 형태 측정학적 분석)

  • Kim Eul-Bae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.4
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    • pp.228-232
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    • 1970
  • From the standpoint of fishery science it is an interesting matter to note that the size of Plecoglessus altivelis population on Cheju Island is smaller than that of the Nakdong River located in the southern part of Korea's mainland. Therefore the author carried out a comparative analysis to determine whether the populations differ because of racial or because of environmental conditions. The morphometric characters of the head length and the body length were used for the analysis of covariance and computed by a standard regression method of testing for the homogeneity of the populations. All samples were collected during 1968 and 1969 in the Milyang Stream of the Nakdong River and the Cheonji Stream of Cheju Island. The results of the analysis of the populations based on the significance test are as follows: 1. Since the F value observed was only 1.023 ($n_1=1,\;n_2=42$) the conclusion was reached that the samples could both belong to the same population. 2. In comparison with the deviations of the regression coefficients between the fish of the Nakdong River and Cherinji Stream, the populations were also highly significant suggesting that thelr belong to the same population. 3. The conclusion is that the fish of Cheju Island became a smaller fish in size due to an environmental condition but not due to racial characteristics.

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Geographic Variations of Three Fulvia mutica Populations

  • Kang, Seo-Kyeong;Yoon, Jong-Man
    • The Korean Journal of Malacology
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    • v.29 no.3
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    • pp.163-169
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    • 2013
  • In the present study, the seven primers BION-33, BION-34, BION-37, BION-41, BION-44, BION-45 and BION-42 generated the total number of loci, average number of loci per lane and specific loci in Hongseong, Yeosu and Goheung population of F. mutica, respectively. 7 primers generated 19 specific loci in the Hongseong population, 29.3 in the Yeosu population and 23.1 in the Goheung population, respectively. Especially, the decamer primer BION-37 generated 7 unique loci to each population, which were identifying each population, approximately 700 bp in Hongseong population. In this study, the dendrogram obtained by the seven primers indicates three genetic clusters: cluster 1 (HONGSEONG 01-HONGSEONG 07), cluster 2 (YEOSU 08-YEOSU 14) and cluster 3 (GOHEUNG 15-GOHEUNG 21). Among the twenty one cockles, the shortest genetic distance that displayed significant molecular differences was between individuals 17 and 19 from the Goheung population (genetic distance = 0.051), while the longest genetic distance among the twenty-one cockle individuals that displayed significant molecular differences was between individuals HONGSEONG no. 03 and YEOSU no. 12 (genetic distance = 0.616). Relatively, individuals of YEOSU population were fairly closely related to that of GOHEUNG population. Ultimately, PCR fragments revealed of in this study may be useful as a DNA marker the three geographic populations to distinguish.