• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.144-144
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• 2017

Micronutrients such as zinc (Zn), iron (Fe), manganese (Mn) have important roles for development and growth in plants but it also have roles in animals and humans. In previous studies, a Korean weedy rice, KH2J was selected to have tolerance to heavy metal, lead (Pb) compared with a cultivar, Milyang23. To identify QTLs for micronutrients concentration in grain, an F2 population (120 plants) were developed from a cross between KH2J and an indica rice cultivar, Milyang23. To measure the concentration of eight ions, Zn, Fe, Mn, Pb, calcium (Ca), copper (Cu), cadmium (Cd) and arsenic (As), grains were collected and digested with 65% nitric acid, and the ion contents were measured using inductively coupled plasma mass spectrometry. A total 27 putative quantitative trait loci (QTLs) were detected on 12 chromosomes by single point analysis and 22 putative QTLs were detected by composite interval mapping. The co-locations of QTL for Zn, Fe and Mn were observed on chromosome 5. The QTLs for Cd, Cu and Zn were co-localized on chromosome 10, and QTLs for Zn, As and Mn was on chromosome 12. The Zn concentration in F2 generation showed significant correlation with concentrations of As (r = -0.4), Cu (r = 0.5) and Fe (r = 0.2) (P < 0.01). Also, the Ca concentration was significantly related with Mn and Fe concentrations (P < 0.01). Fine mapping of these QTLs is underway to analyze their functional relationship.

• Journal of Sericultural and Entomological Science
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• v.35 no.2
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• pp.100-104
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• 1993

The genetics analysis for weight of matured silkworm(WMS) and number of eggs produced per moth(NEM) was studied by the seven parents diallel. Mean squares of additive effect, dominant effect, maternal effect and reciprocal effect were significant for two characters observed. The component of genetic variance analysis for WMS and NEM showed that dominant effect was higher than additive effect. Narrow sense heritability(h2ns) estimates were 0.773 and 0.228, in the WMS and NEM. The estimate of broad sense heritability(h2bs) value was higher than that of h2ns because of the low importance of dominance effect. Incomplete dominance was shown by Vr-Wr graphic analysis in the weight of matured silkworm and overdominance in the number of eggs produced per moth. In general combining ability effect, Jam 107 and Jam 124 was showed positively high for WMS and Jam 107 and S1 was expressed positively high for NEM. In specific combining ability effect, hybrids in S1XC51, S1XJam124 and Jam 107XJam 108 were exhibited positively high for WMS and Jam 107XN63, S1XC51, N74XJam 108 and Jam 107XJam 108 were found positively high for NEM.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.5
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• pp.699-706
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• 2020

Predictive models in food microbiology are used for predicting microbial growth or death rates using mathematical and statistical tools considering the intrinsic and extrinsic factors of food. This study developed predictive growth models for Bacillus cereus on dried laver Pyropia pseudolinearis stored at different temperatures (5, 10, 15, 20, and 25℃). Primary models developed for specific growth rate (SGR), lag time (LT), and maximum population density (MPD) indicated a good fit (R²≥0.98) with the Gompertz equation. The SGR values were 0.03, 0.08, and 0.12, and the LT values were 12.64, 4.01, and 2.17 h, at the storage temperatures of 15, 20, and 25℃, respectively. Secondary models for the same parameters were determined via nonlinear regression as follows: SGR=0.0228-0.0069*T₁+0.0005*T₁²; LT=113.0685-9.6256*T₁+0.2079*T₁²; MPD=1.6630+0.4284*T₁-0.0080*T₁² (where T₁ is the storage temperature). The appropriateness of the secondary models was validated using statistical indices, such as mean squared error (MSE<0.01), bias factor (0.99≤B_f≤1.07), and accuracy factor (1.01≤A_f≤1.14). External validation was performed at three random temperatures, and the results were consistent with each other. Thus, these models may be useful for predicting the growth of B. cereus on dried laver.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.268-268
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• 2022

Protein is a major nutrient of food and has long been studied for nutritional and utility value. Among them, rice protein is attracting attention because of its hypoallergenic characteristics and nutritional value. Mutant DM225 with increased protein content was selected by EMS treatment on the weed rice Dharial. QTL analysis of Protein content was carried out using BC₂F₂ populations derived from a cross between "Hanareum2" as a recurrent parent and "DM225" as a donor parent. The protein content of populations was between 5~11%, with an average of 7.7%. To identify QTLs related to Protein content, 117 KASP markers(polymorphic ratio: 15%) showing polymorphisms between the parents were genotyped for the BC₂F₂ population. One QTL was detected between markers SK07 06 and SK07_10 on chromosome 7(LOD: 28.1). This QTL explained 71.4% of the phenotypic variance for Protein content. This QTL will be useful for protein-related rice breeding program.

• Journal of Animal Science and Technology
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• v.65 no.4
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• pp.735-747
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• 2023

The composition of fatty acids determines the flavor and quality of meat. Flavor compounds are generated during the cooking process by the decomposition of volatile fatty acids via lipid oxidation. A number of research on candidate genes related to fatty acid content in livestock species have been published. The majority of these studies focused on pigs and cattle; the association between fatty acid composition and meat quality in chickens has rarely been reported. Therefore, this study investigated candidate genes associated with fatty acid composition in chickens. A genome-wide association study (GWAS) was performed on 767 individuals from an F2 crossbred population of Yeonsan Ogye and White Leghorn chickens. The Illumina chicken 60K significant single-nucleotide polymorphism (SNP) genotype data and 30 fatty acids (%) in the breast meat of animals slaughtered at 10 weeks of age were analyzed. SNPs were shown to be significant in 15 traits: C10:0, C14:0, C18:0, C18:1n-7, C18:1n-9, C18:2n-6, C20:0, C20:2, C20:3n-6, C20:4n-6, C20:5n-3, C24:0, C24:1n-9, monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA). These SNPs were mostly located on chromosome 10 and around the following genes: ACSS3, BTG1, MCEE, PPARGC1A, ACSL4, ELOVL4, CYB5R4, ME1, and TRPM1. Both oleic acid and arachidonic acid contained the candidate genes: MCEE and TRPM1. These two fatty acids are antagonistic to each other and have been identified as traits that contribute to the production of volatile fatty acids. The results of this study improve our understanding of the genetic mechanisms through which fatty acids in chicken affect the meat flavor.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.2
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• pp.169-175
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• 2007

The Bio Best Bacillus(B3) and Rotating Activated Bacillus Contactor(RABC) processes, in which Bacillus strains are predominating, are reported to remove nitrogen and phosphorus as well as organic matter effectively. Nevertheless the nutrient removal characteristics of the Bacillus strains have not been studied in detail so far. This study investigated the organic and nutrient removal by Bacillus strains, Bacillus megaterium(KCTC 3007), Paenibacillus polymyxa(KCTC 3627), and Bacillus sp. A12, C21, F12, and L1(isolated from a B3 process), by incubating the strains in 0.2% nutrient broth at $30^{\circ}C$. Burkholderia cepacia(KCTC 2966), a common activated sludge organism, was used as a reference species for comparison. Although the degradation rate was affected by the population sire, the specific removal rates of organic matter by Bacillus strains were greater by $2\sim5$ times than that of Burkholderia. In particular, the culture bottles inoculated with the endospores of Bacillus megaterium and Bacillus sp. C21, F12, and N12 showed significantly higher degradation rate than those of vegetative cells.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.359-369
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• 2018

Harmful algal blooms (HABs) are a serious problem for public health and fisheries industries, thus there exists a need to investigate the possible ways for effective control of HABs. In the present study, we investigated the algicidal effects of a newly developed GreenTD against the HABs (Chattonella marina, Heterosigma akashiwo, Cochlodinium polykriokides, and Heterocapsa circularisquama) and non-HABs (Chaetoceros simplex, Skeletonema sp. and Tetraselmis sp.), which is focused on the different population density and concentration gradients of algicidal substances. The time series viability of target alga was assessed based on the activity of Chl. a photosynthetic efficiency in terms of $F_v/F_m$, and in vivo fluorescence (FSU). Effective control of Raphidophyta, C. marina and H. akashiwo was achieved at a GreenTD concentration of $0.5{\mu}gL^{-1}$ and $0.2{\mu}gL^{-1}$, respectively, and regrowth of both the species was not observed even after 14 days. The inhibitory ratio of the dinoflagellate, C. polykriokides was more than 80% at $0.2{\mu}gL^{-1}$ of GreenTD. H. circularisquama was constantly affected in the presence of $0.2{\mu}gL^{-1}$ of GreenTD in the high- and low-population density experimental groups. On the other hand, diatoms, C. simplex, and Skeletonema sp. were not significantly affected even in the presence of $0.2{\mu}gL^{-1}$ of GreenTD and exhibited re-growth activity with the passage of incubation time. In particular, green alga Tetraselmis sp. remained unaffected even in the presence of the highest concentration of GreenTD ($1.0{\mu}gL^{-1}$), implying that non-HABs were not greatly influenced by the algicidal substances. As a result, the algicidal activity of GreenTD on the harmful and nonharmful algae was as follows: raphidophyte>dinoflagellates>diatoms>green alga. Consequently, our results indicate that inoculation of GreenTD substances into natural blooms at a threshold concentration ($0.2{\mu}gL^{-1}$) can maximize the algicidal activity against HABs species. If we consider the dilution and diffusion rate in the field application, it is hypothesized that GreenTD will demonstrate economic efficiency, thus leading to effective control against the target HABs in the closed bay.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.3
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• pp.255-263
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• 2014

We investigated the reproductive characteristics of Lycodes tanakae in the coastal waters of the middle East Sea to elucidate the species' population structure. We investigated Lycodes tanakae maturation and spawning based on samples collected by Danish seine and gill nets from January 2012 to December 2013. We analyzed monthly changes in maturity stage, gonadosomatic index (GSI), egg diameter, fecundity, and total length at 50% group maturity. The spawning period was December to February, while fecundity ranged from 1,677 eggs at 57.3 cm (total length;TL) to 6,445 eggs at 75.7 cm. The relationship between TL and fecundity (F) was $F_e=6E-05TL^{3.127}$ ($R^2$ = 0.516), and F increased with increasing TL. We estimated the TL at 50% group maturity as 60.4 cm for females and 59.8 cm for males. This study is the first report of Lycodes tanakae reproductive characteristics in the coastal waters of the middle East Sea.

• Journal of the Korean Institute of Landscape Architecture
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• v.29 no.1
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• pp.80-91
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• 2001

The purpose of this research is to provide a basic knowledge to open up for undeveloped park in process of the park policy establishment by setting up the priority of park development and valuation factor, valuation index as well as building a basic material to undeveloped neighborhood park in Daegu Metropolitan City. Thirteen urban neighborhood parks(1,841,663$m^2$) in the Daegu Metropolitan City were selected for this study. Analyzing and reviewing the previous research and literature review were conducted, and the evaluation index and analysis criteria were constructed to decide the development priority of parks. The results of this research are as follows; 1) Following to the result of the expert questionnaire test in order to estimate weight of valuation factors. It had a high score in available population, and estimated amount of the gross compensation, unpurchased land rate. On the other hand park rate of autonomous district, altitude, rate of population increase in autonomous district and a low score. 2) Generally, most f the plain type parks had a high score in the Sea level, land using pattern, an estimated amount of the gross compensation, but hill type parks had a high score the rate of green covered, park area for one person of administration building. 3) Daebong, Soosung, Dosan, 2.28 youth, Kyungnam parks were selected high priority. Soochang, Donsibong, Janggi, Dongin parks were selected middle priority. Bokhyun, Galsan, Manchon, Gusoosan parks, which were selected low priority. This research for undeveloped park is a basic research step to open up. Three many difficult things in shortage of the basic material for building valuation factor and measuring of the method measurement. The application of weight which make a decision importance among the valuation factor should have ore reasonable study and more scientific research from now because there still remains a lot of shortage of positive materials in there.

• The Plant Pathology Journal
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• v.38 no.6
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• pp.572-582
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• 2022

Sheath blight disease caused by the necrotrophic, soilborne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 rice landraces were screened against R. solani under artificial epiphytotics and identified six moderately resistant landraces, Jigguvaratiga, Honasu, Jeer Sali, Jeeraga-2, BiliKagga, and Medini Sannabatta with relative lesion height (RLH) range of 21-30%. Landrace Jigguvaratiga with consistent and better level of resistance (21% RLH) than resistant check Tetep (RLH 28%) was used to develop mapping population. DNA markers associated with ShB resistance were identified in F₂ mapping population developed from Jigguvaratiga × BPT5204 (susceptible variety) using bulk segregant analysis. Among 56 parental polymorphic markers, RM5556, RM6208, and RM7 were polymorphic between the bulks. Single marker analysis indicated the significant association of ShB with RM5556 and RM6208 with phenotypic variance (R²) of 28.29 and 20.06%, respectively. Co-segregation analysis confirmed the strong association of RM5556 and RM6208 located on chromosome 8 for ShB trait. This is the first report on association of RM6208 marker for ShB resistance. In silico analysis revealed that RM6208 loci resides the stearoyl ACP desaturases protein, which is involved in defense mechanism against plant pathogens. RM5556 loci resides a protein, with unknown function. The putative candidate genes or quantitative trait locus harbouring at the marker interval of RM5556 and RM6208 can be further used to develop ShB resistant varieties using molecular breeding approaches.