• Journal of Plant Biotechnology
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• v.46 no.2
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• pp.71-78
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• 2019

The objective of this study was to use 'Danta PR', NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, 'Hyangseo 1' and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of 'Danta PR' BC1 (F1 x 'Danta PR') population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties.

• BMB Reports
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• v.43 no.8
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• pp.530-534
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• 2010

Cytochrome P450 (CYP) 1A2 gene polymorphisms are thought to be involved in the metabolism of theophylline (TP). We aimed to investigate the effect of CYP1A2*1C, CYP1A2*1D, CYP1A2*1E, and CYP1A2*1F polymorphisms of the CYP1A2 on TP metabolism by PCR-RFLP in 100 Turkish patients with chronic obstructive pulmonary disease (COPD) receiving TP. One hundred and one healthy volunteers were included as control group. The genotype frequencies of the CYP1A2*1D and CYP1A2*1F were found to be significantly different in the patients compared to the controls. The "T" allele at -2467 delT and the "C" allele at -163 C > A in the CYP1A2 displayed association with a significantly increased risk for COPD. "T" allele at -2467 delT was also associated with a high risk of disease severity in COPD. In conclusion, our data suggest that genetic alterations in CYP1A2 may play a role both in the pharmacogenetics of TP and in the development of COPD.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.9 no.1
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• pp.133-140
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• 2014

The purpose of this research was to identify how service composition factor impact organizational performance of long-term care facilities for elderly people. The target population was the staffs who were working at long-term care facilities for elderly people in urban areas of Seoul, Inchon and cities in Kyonggi-do as of September 2012. Independent variables, main factors for organizational performances, are leadership, educational training, compensation, initiative, and service quality. Dependent variables are selected as duty satisfaction, job performance, financial aptitude and beneficiary-oriented policy. SPSS ver18.0 statistical computer program was conducted in order to analyze the multivariate statistical data. The results are examined in detail in terms of the influence of two managing systems in the senior-care service on the organizational performance and the evaluations of the relation and difference caused by the input variables in two managing systems. In one case of the influence of two managing systems in the senior-care service on the organizational performance, the findings show 1) the influence of the service managing factor is clearly related to duty satisfaction with the result of 0.001 (F=37.429) regression data, 2) the influence of the service managing factor is clearly related to job performance with the result of 0.001 (F=55.099) regression data, 3) the influence of the service managing factor is closely related to financial aptitude with the result of 0.001 (F=56.904) regression data, and 4) the influence of the service managing factor is also clearly related to beneficiary-oriented policy with the result of 0.001 (F=61.367) regression data.

• The Korean Journal of Ecology
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• v.27 no.5
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• pp.269-281
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• 2004

The survey of birds in Nakdong River Basin was conducted from March in 2003 to February in 2004. This study shows that the population of birds was 94,481 individuals and 114 species. The bird recorded in Daemadeung (Ⅰ) was 21,781 individuals and 55 species. It is surrounded with silt to have a better condition for a habitat and the rest place than other areas in the Nakdong River Basin. In the traits of the groups, Grebes were well established in the lower estuary(Eulsook islet), Cormorants in the main stream of the Nakdong River and around West Nakdong River (F). Swans, Shelduck and Shorebirds were using silt in the lower estuary where the depth of water is shallow, dabbling ducks were also established in West Nakdong River (F) and Yummak (C), diving ducks in the upper Nosan Watergate in West Nakdong River (F). Besides, land birds was distributed in the Yummak (C), Eulsook islet (A) and West-Nakdong River (F). Because they are connected with the artifical forest in Eulsook Islet Garbage Field and Ⅱwoong islet, it is good for the land birds and the water birds to have a habitat in this kinds of vacant lot. The Nakdong River Basin is not only an important area as the habitat and rest place for ducks and geese but also one of the areas where we can not get out of the temptation to development. To preserve effectively the natural habitat from human s intervention, it needs to keep supervising in Daejeo Watergate (E) and the upper area Noksan watergate in West Nakdong River (F) and Yummak (C).

• Korean Journal of Ichthyology
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• v.19 no.3
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• pp.179-184
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• 2007

Maturity and spawning of spinyhead sculpin, Dasycottus setiger were investigated using samples collected by the eastern sea danish seine and gillnet in the East Sea of Korea from January to December 2004. We analyzed monthly changes in maturity stages, gonadosomatic index (GSI), egg diameter, fecundity, total length at 50% group maturity, and sex ratio. GSI began to increase in August, reached a maximum between September and November, and decreased in December. The spawning period was August to December, and the main spawning period was September to November. The fecundity ranged from 6,210 eggs at 22.3 cm (TL) to 15,898 eggs at 32.2 cm (TL). The relationship between total length (TL) and fecundity (F) was $F=25.475TL^{1.818}$ ($R^2=0.761$), and F increased with TL. The TL at 50% group maturity was estimated to be 23.4 cm. The sex ratio was 1 : 1.3 (female : male), indicating the male population was seem to be slightly predominant.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.689-693
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• 2011

The purpose of this study was to investigate the Cytochrome P450 (CYP2A6) gene as a candidate gene for the traits related with meat fatty acid composition traits in pigs. Porcine CYP2A6 polymorphisms were detected and PCR-RFLP was performed for genotyping of Korean native pig (n=14), Landrace (n=3), Duroc (n=3), Berkshire (n=3), Yorkshire (n=8) and F2 population composed of 202 individuals from an intercross between Korean Native pig and Yorkshire. PCR primer set amplified a 612 bp fragment of CYP2A6 and digestion of the PCR products was performed with the restriction enzymes SchI. The CYP2A6 SchI polymorphism was only found in the KNP breed. The genotype frequencies of TT, TC and CC genotypes were 0.36, 0.56 and 0.08 in the KNP respectively and the other pig breeds were fixed with CC genotype (Duroc, Landrace, Berkshire and Yorkshire). Statistical association between genotypes and fatty acid composition traits were tested in the Korean native pig and Yorkshire crossed F2 pigs. The CYP2A6 SchI polymorphism was associated with only fatty acid composition C20:3n3 level (cis11,14,17-Eicosatrienoic acid, p=0.0252). The 'T' allele was associated with lower C20:3n3 level. Further study is required to validate the genotypic association and biological consequence of the CYP2A6 gene polymorphism in pigs.

• Journal of Crop Science and Biotechnology
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• v.11 no.4
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• pp.243-248
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• 2008

The bean bug Riptortus clavatus Thunberg (Heteroptera: Alydidae) is an important pest, causing serious yield loss in soybean. But the information on mechanism of resistance to R. clavatus is limited. The objective of this study was to identify QTLs for R. clavatus resistance using simple sequence repeat (SSR) markers in a soybean population of recombinant inbred lines (RILs) developed from the cross PI 171451 ${\times}$ Hwaeomputkong. A genetic map from this population was constructed with a total of 136 SSR markers covering 1073.9 cM on 20 linkage groups (LGs). With 126 $F_5$ RILs, two independent QTLs for resistance to R. clavatus were mapped on LGs B1 and C2. The amount of phenotypic variation explained by these QTLs ranged from 12 to 16%. PI 171451 showed an escape response to R. clavatus. Under feeding conditions, 14.4% of RILs showed greater resistance to R. clavatus than the resistant parent. The resistance to R. clavatus in soybean from PI 171451 was incomplete and quantitatively inherited and the QTLs for resistance to R. clavatus detected in the RIL population were not significantly affected by epistatic interactions.

• Childhood Kidney Diseases
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• v.22 no.2
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• pp.37-41
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• 2018

Purpose: Familial renal glucosuria (FRG, OMIM #233100) is a rare but relatively benign genetic condition characterized by persistent isolated glucosuria with a normal blood glucose level. We report three additional SLC5A2 mutations and examine their phenotypic and genetic characteristics in a Korean FRG cohort. We also reviewed the literature and summarized the genotypes of all Korean patients with FRG. Methods: A genetic analysis was conducted by directly sequencing all 14 exons of the SLC5A2 gene and their flanking regions in six unrelated Korean children with FRG and their family members. Novel non-synonymous single-nucleotide polymorphisms were identified and compared with known mutations that are repeatedly detected in the Korean population. Results: We found two novel mutations [c.274G>A (G92S) and c.1168C>T (L390F)] and one known [c.1382G>A (S461N)] mutation in each family and one recurrent mutation [c.1346G>A (G449D) (rs768392222)] in two pedigrees. The recurrent G449D was predicted to be "possibly damaging," with a score of 0.883 in Polyphen-2, while G92S, L390F, and S461N were predicted to be "probably damaging," with scores of 1.000, 0.999, and 0.996, respectively. Conclusions: Two novel, one previously reported, and one recurrent mutation were identified in six Korean FRG pedigrees as causative mutations of renal glucosuria. Sequence variations in the SLC5A2 gene were frequently detected in children with persistent isolated glucosuria. A long-term follow-up of this FRG cohort is needed to understand how these specific SGLT2 mutations impair kidney function and energy homeostasis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.3
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• pp.460-468
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• 2017

The purpose of this study was to compare the effects of class-based and simulation-based CPR education for the rural elderly on their attitudes toward CPR, self-efficacy, and satisfaction. Using a quasi-experimental design with a nonequivalent control group, this study used a pretest-posttest method with a sample of 43 elderly people aged 65 or older in rural areas. The twenty-two elderly people in the control group received class-based CPR education, while the experimental group underwent both class-based and simulation-based education. Both groups were given surveys to complete before and after the education. The statistical analysis was based on the $X^2$-test, Fisher's exact test, paired t-test, and ANCOVA. The study found no significant differences between the two groups in terms of their attitudes (F=0.89, p=.353). However, statistical differences were found in the self-efficacy (F=5.54, p=.025) and satisfaction (F=4.66, p=.039) between the two groups. This study provides the baseline data indicating that the education methods implemented in this study were effective for the elderly living in rural areas. Thus, it is necessary to make efforts to ensure that the elderly are able to manage emergency situations by boosting their CPR self-efficacy and educational satisfaction through simulation-based education.

• Korean journal of applied entomology
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• v.31 no.1
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• pp.50-68
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• 1992

The results of survey on the nematodes associated with sapling from 8 Forestry Experiment Stations(F.E.S.) in Korea are reported herein. A total of 35 species under 20 genera, 11 families, and 2 orders were identified from the soils around the roots of 119 tree species and amongst them, Xiphinema insigne is unrecorded species for Korea and Geocenamus sp. is a new species. Tylenchorhynchus claytoni, which showed the highest detection rate (D.R.) from all F.E.S., except Nambu F.E.S, was the dominant species among the nematodes associated with saplings. The D.R. of T. claytoni was the highest in Ch’ungnam F.E.S. by 100% from 15 saplings sampled, followed by 87.5% in Kangwon, 66.6% in Kyongbuk, 58.3% in Cheonbuk, 48.1% in Ch’ungbuk, 41.3% in Kyongki, and 40% in Cheonnam F.E.S. Other important nematode species which showed very high densities by stations are; Criconemella morgensis (D.R.: 37.5%), Helicotylenchus digonicus (D.R. : 35%), Pararotylenchus pini (D.R.: 37%), Tylenchorhynchus nudus (D.R.: 37.9%), Criconemella informis (D.R.: 27.5%), and Meloidogyne sp. (D.R.: 12.5), from Chunbuk, Chunnam, Ch’ungbuk, Kyongki, and Kangwon F.E.S., respectively.