• Korean Journal of Breeding Science
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• 제42권5호
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• pp.507-514
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• 2010

Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

• Asian-Australasian Journal of Animal Sciences
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• 제30권1호
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• pp.1-7
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• 2017

Objective: Three genome-wide association studies (GWAS) and a meta-analysis of GWAS were conducted to explore the genetic mechanisms underlying variation in pig teat number. Methods: We performed three GWAS and a meta-analysis for teat number on three pig populations, including a White Duroc${\times}$Erhualian $F_2$ resource population (n = 1,743), a Chinese Erhualian pig population (n = 320) and a Chinese Sutai pig population (n = 383). Results: We detected 24 single nucleotide polymorphisms (SNPs) that surpassed the genome-wide significant level on Sus Scrofa chromosomes (SSC) 1, 7, and 12 in the $F_2$ resource population, corresponding to four loci for pig teat number. We highlighted vertnin (VRTN) and lysine demethylase 6B (KDM6B) as two interesting candidate genes at the loci on SSC7 and SSC12. No significant associated SNPs were identified in the meta-analysis of GWAS. Conclusion: The results verified the complex genetic architecture of pig teat number. The causative variants for teat number may be different in the three populations

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1425-1428
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• 2001

This study was conducted to investigate the effects of supplementation of a probiotics complex on growth performance, nutrient digestibility, diarrhea score and microbial population in pigs weaned at 21 days of age. Treatments were 1) control A (0.2% antibiotics, Avilamycin), 2) control B (0.1 % $Ractocom^{(R)}$), 3) 0.1%, 4) 0.2% and 5) 0.3% probiotics complex; 80 pigs were used and each treatment had 4 replicates with 4 pigs per replicate (16 pigs per treatment). During phase I period (d 0 to 14), although there was no significant difference, pigs fed control B diet showed higher ADG (average daily gain) and better F/G (feed/gain) than any other treatments. During late experimental period (d 15 to 28), pigs fed diet supplemented with 0.2% probiotics complex showed slightly higher ADG. Overall (d 0 to 28) the diet that contained 0.2% probiotics complex gave slightly higher ADG and ADFI (average daily feed intake) than the other diets. In a metabolic trial using 20 piglets, nutrient digestibility showed the best results in pigs fed 0.2% probiotics complex diet, but not significantly different from other groups. Diarrhea score and microbial population status in intestine, colon and feces were not affected by dietary treatments. In conclusion, this study suggested that a newly developed probiotics complex can replace antibiotics in weaned pigs.

• Journal of Animal Science and Technology
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• 제50권5호
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• pp.621-632
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• 2008

Genetic polymorphisms was investigated at five single nucleotide polymorphisms(SNP) sites in four porcine genes(ADCYAP1R1, FABP3, MC4R, and MYL2) and analyzed their statistical association with growth traits in F2 reciprocal-crossbred population between Landrace and Jeju native black pig(JNP). All populations, JNP, Landrace and their F2 were polymorphic for all five SNP loci tested, however, the homozygote T/T of FABP3 g.-158T>C and the homozygote G/G of ADCYAP1R1 intron 2 337A>G were not found in JNP and Landrace, respectively. The genotypes of ADCYAP1R1 were significantly associated with body weights(BW) at 3 weeks and at 20 weeks(P<0.05), respectivley, those of FABP3 g.-135delT were associated with late average daily gain(LADG) (P<0.01), and those of FABP3 g.-158T>G were associated with body weights during late growth period such as, BW20 and LADG(P<0.01). Those of MC4R were also significantly associated with BW10 suggesting by the difference of early average daily gain(EADG) (P<0.05), and with LADG(P<0.01). The body length of F2 animals was affected by the genotypes of ADCYAP1R1, MC4R, and MYL2(P<0.05), respectively. Among these, MC4R A/A homozygotes showed over 3 cm longer in body length than those of other genotypes. As the useful basic information, these results suggested that SNP markers showing statistical association with growth traits and the results help to select the sires of JNP for improving the productivity in JNP-related crossbreeding system in pig industry and also to construct the molecular breeding system for breed improvement of JNP itself.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제51권2호
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• pp.169-172
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• 2006

Leaflet number of soybean controlled by Lf2 locus is the important trait in photosynthesis and plant type. The objective of this research was to identity molecular markers linked to the lf2 locus. A total of $115F_2$ plants were derived from a cross between normal three-leaflet type Sinpaldalkong (Lf2Lf2) and seven-leaflet mutant type T255 (lf2lf2). All leaflet counts of parents and $F_2$ individual plants were made in the field on fully expanded leaves on the main stem when terminal growth of the main stem had ceased. One-thousand 10-mer oligonucleotide RAPD primers and 664 SSR primers were used. The segregation ratios of 3 : 1 were observed in the $F_2$ population and the Chi-square values strongly suggested that the seven-leaflet was controlled by a single recessive gene. A genetic map was constructed from the 15 segregating markers (9 RAPDs, 5 SSRs, 1 lf2 locus). OPAD03 and OPAI13 RAPD markers were linked to the lf2 locus that controlled seven-leaflet type at a distance of 20.5 and 23.5 cM, respectively. Molecular markers identified in this study linked with lf2 locus will be helpful to locate lf2 locus on the public soybean molecular linkage map and would be useful for tagging the lf2 locus that controls seven-leaflet trait.

• Journal of Korean Academy of Fundamentals of Nursing
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• 제4권2호
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• pp.283-300
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• 1997

This study was undertaken to grasp health-promoting behavior of the elderly and to identify variables related to them in order to facilitate nursing intervention for health promotion of this population. The subjects for this study were 291 old persons obtained by cluster sampling from twenty general social welfare centers located in Tague. Data were collected by self-reported questionnaires from August 13 to September 13, 1996. Questionnaires were developed based on Sherer and others' Self-Efficacy scale, Rosenberg's Self-Esteem scale, Wallston and other's Multidimensional Health Locus of Control scale, Northern illinois University's Helath Self Rating Scale, Walker and others' Health Promotion Lifestyles Profile. Analysis of the data was done by use of descriptive statistics, stepwise multiple regression, Pearson Correlation Coefficient, MANOVA, t-test, and ANOVA. The results were summarized as follows : 1. For the practice of health-promoting behavior, the mean score was 2.89 and range was 3.59 to 2.09. The factor of the highest mean score was regular diet(M=3.42) and factor of the lowest mean score as stress management(M=2.27). 2. The combination of self-efficacy, internal health locus of control, family number, and average monthly pocket money explained 30.0% of the variance of health-promoting behavior. 3. With regard to the relationship between health-promoting behavior and cognitive perceptual factor, self-efficacy correlated positively with health-promoting behavior(r=.4951, P=.0001), self-esteem correlated positively(r=.3263, P=.0001), internal health locus of control correlated positively(r=.3244, P=.0001), perceived health status correlated positively(r=.1355, P=.0274). 4. According to age(F=2.50, P=.0431), sex(t=2.14, P=.0332), marital status(F=7.85, P=.0005), education(F=5.44, P=.0003), family number(F=11.18, P=.0001), people living together(F=7.21, P=.0009), previous occupation(F=5.83, P=.0001), average monthly pocket money(F=7.27, P=.0001), there were differences of health-promoting behavior. The above findings show that health-promoting behavior are related to demographic characteristics, four cognitive perceptual factors(self-efficacy, self-esteem, internal health locus of control, perceived health status). On the basis of the above findings the following recommendations are made ; 1. Nursing interventions enhancing exercise or activity, accountability for health, stress management of the elderly must be provided. 2. Nursing strategies enhancing self-efficacy which is most significant effect on health-promoting behavior must be developed.

• The Korean Journal of Ecology
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• 제23권2호
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• pp.181-185
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• 2000

This study was carried out to see whether Gastrophysa viridula (Degeer) (Coleoptera : Chrysomelidae) could be used as a biological control agent for Rumex obtusifolius L., with human manipulation of the beetle population. The study was consisted of three experimental sets : Wet-Dry experiment (the wet weight Vs dry weight relationship of Rumex obtusifolius L.), Greenhouse feeding experiment, and Field experiment. There was a significant correlation between the total wet and dry weight of Rumex obtusifolius as follows : Total dry weight : -0.23542+ (0.17514${\times}$Total wet weight) ($R^2$=0.9317, p=0.047, T=16.927 (dF=21)). In the Greenhouse feeding experiment, the result was very promising. The relationship between the density unit of the beetles and the growth of the plant is given below (20 day) : Plant growth =105.8+(-34.4${\times}$Density unit) ($R^2$=0.76, p=0.13). A repeated introduction of the beetle population into the field vegetation of R. obtusifolius from April to October is suggested to see the beetle's grazing ability on the plant. This study shows that the potential grazing power of the beetle on Rumex obtusifolius was enough to defoliate the plants, but it was able to recover from its root reserves. The practical question remains as to whether repeated additions (by man) of the beetles to Rumex obtusifolius could eliminate them.

• International Journal of Reliability and Applications
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• 제7권2호
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• pp.177-186
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• 2006

Typical confidence intervals for a mean or mean residual life (MRL) are centered about the mean or mean residual life. We discuss novel confidence intervals that produce statements like "we are 95% confident that the MRL function, e(t), is greater than a prespecified $\mu_o$ for all t in the interval [0, $\hat{\theta})$)" where $\hat{\theta}$ is determined from the sample data, confidence level, and $\mu_o$. Also, we can have statements like 'we are 95% confident that the MRL of population 1, namely $e_1$(t), is greater than the MRL of population 2, $e_2$(t), for all t in the interval [0, $\hat{\theta}$)" where $\hat{\theta}$ is determined from the sample data and confidence level. We illustrate these one and two sample confidence intervals on internal bonds (tensile strengths) for an important modem engineered wood product, called medium density fiberboard (MDF), used internationally.

• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.669-676
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• 2007

This study was conducted to detect quantitative trait loci (QTL) affecting growth and body composition in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 18 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between the models were used to characterize QTL for mode of expressions. A total of 8 (39) QTL were detected at the 5% genome (chromosome)-wise level for the 17 analyzed traits. Of the 47 QTL detected, 21 QTL were classified as Mendelian expressed, 13 QTL as paternally expressed, 6 QTL as maternally expressed, and 7 QTL as partially expressed. Of the detected QTL at 5% genome-wise level, two QTL had Mendelian mode of inheritance on SSC6 and SSC9 for backfat thickness and bone weight, respectively, two QTL were maternally expressed for leather weight and front leg weight on SSC6 and SSC12, respectively, one QTL was paternally expressed for birth weight on SSC4, and three QTL were partially expressed for hot carcass weight and rear leg weight on SSC6, and bone weight on SSC13. Many of the Mendelian QTL had a dominant (complete or overdominant) mode of gene action, and only a few of the QTL were primarily additive, which reflects that heterosis for growth is appreciable in a cross between Korean native pig and Landrace. Our results indicate that alternate breed alleles of growth and body composition QTL are segregating between the two breeds, which could be utilized for genetic improvement of growth via marker-assisted selection.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1507-1510
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• 2012

Quantitative trait loci (QTL) associated with fat deposition traits in pigs are important gene positions in a chromosome that influence meat quality of pork. For QTL study, a three generation resource population was constructed from a cross between Korean native boars and Landrace sows. A total of 240 F2 animals from intercross of F1 were produced. 80 microsatellite markers covering chromosomes 1 to 10 were selected to genotype the resource population. Intervals between adjacent markers were approximately 19 cM. Linkage analysis was performed using CRIMAP software version 2.4 with a FIXED option to obtain the map distances. For QTL analysis, the public web-based software, QTL express (http://www.qtl.cap.ed.ac.uk) was used. Two significant and two suggestive QTL were identified on SSC 6, 7, and 8 as affecting body fat and IMF traits. For QTL affecting IMF, the most significant association was detected between marker sw71 and sw1881 on SSC 6, and a suggestive QTL was identified between sw268 and sw205 on SSC8. These QTL accounted for 26.58% and 12.31% of the phenotypic variance, respectively. A significant QTL affecting IMF was detected at position 105 cM between markers sw71 and sw1881 on SSC 6.