• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.671-676
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• 2008

Forty-eight Naemi lambs (avg. BW 31.7 kg) were transported by truck for a distance of 1,450 km from Al-Jouf to Riyadh, Saudi Arabia. On arrival day, the lambs were randomly allocated to four groups receiving diets supplemented with 0.0, 0.3, 0.6 and 0.9 ppm organic chromium (Cr). Each group consisted of four separately housed replicates of three lambs each. The animals were fed ad libitum on a grower diet for 84 days. Blood samples were obtained shortly before transportation, upon arrival and at weekly intervals thereafter from all lambs for analysis of plasma and serum. Plasma glucose and serum cortisol, total protein, albumin, urea-N and total cholesterol concentrations were determined. A cursory clinical examination of the lambs, along with rectal temperature, was undertaken at different intervals during the experiment. The lambs were inoculated each with 2 ml i.v. chicken red blood cells (CRBC) on days 0, 21, and 42. Serum total, IgG and IgM antibody titers were determined at weekly intervals post-immunization. An in vivo intradermal hypersensitivity test was carried out on 6 lambs from each group on days 10 and 70. Transportation of the lambs resulted in a significant (p<0.001) elevation of serum cortisol, total protein and albumin levels, as well as increased plasma glucose concentration, with corresponding decrease in total cholesterol, while blood urea-N remained largely unchanged. These constituents returned to normal levels during subsequent weeks, with no significant differences in their concentrations being observed between the Cr-supplemented groups and controls. Rise in rectal temperature after transportation was reduced to a greater extent (p<0.05) in Cr-supplemented versus control lambs. Total, IgG and IgM antibody titers against CRBC rose significantly (p<0.05) during immunizations in all groups, with significantly and linearly higher (p<0.05) total and IgG titers in Cr-supplemented versus control lambs. By contrast, no significant effect due to Cr supplementation was recorded in IgG titers, which increased equally in Cr-fed and control groups. Skin thickness in response to intradermal inoculation of phytohaemagglutinin (PHA) was also significantly (p<0.01) increased as a result of Cr supplementation. These results indicate that dietary Cr supplementation might be useful during stress especially for enhancing immune responses in transport-stressed lambs.

• Journal of Dairy Science and Biotechnology
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• v.32 no.1
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• pp.47-53
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• 2014

In this study, our aim was to investigate the changes in ginsenosides and polyphenols in red ginseng extract fermented by Lactobacillus acidophilus and to manufacture fresh cheese using fermented red ginseng extract. Red ginseng extract (3%, w/v) was fermented by L. acidophilus for 24 h. On performing lactic acid bacteria counts, we determined that L. acidophilus reached its maximum growth phase after 16 h; this was followed by decrease in growth. During fermentation, the levels of ginsenosides Rg3 (20S) and Rg3 (20R) as well as protopanaxadiol (20R), F1, and compound K increased, while those of s Rb2, Rd, Rf, and Rg1 decreased. The pH, titratable acidity, and viable cell counts in fresh cheese prepared using fermented red ginseng extract were measured during the storage period. The pH decreased over time, while titratable acidity and viable cell counts increased with increase in the duration of the storage period. Sensory tests showed that the overall sensory properties of fresh cheese prepared using 1% fermented red ginseng extract were similar to those of the control groups. This result suggests that L. acidophilus-fermented red ginseng has potential for development as a new bioactive material.

• The Korean Journal of Ecology
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• v.16 no.3
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• pp.239-259
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• 1993

The vegetation types and distributional characteristics of the forest vegetation on Mt. Ilwol were investigated by the Z-M school method. Geographical distribution of the vegetation was a central/montane(CEMO) type of the Korean Peninsula subtype. The forest was classified into five vegetation units: Artemisio-Quercetum mongolicae (Artemisio-Quercetum typicum subass. nov., Artemsio-Quercetum pinetosum dens~florae subass. nov. ). Lychno-Quercetum mongolicae (Lychno-Quercetum Calamagrostetosum arundinaceae subass. nov.. Lychno-Quercetum ligularetosum f~scherii subass. nov.), Fraxinus rhynchophylla community, Quercus variabilis community and Larix leptolepis afforestation. Species with percentage constance degree more than 61% are Quercus mongolica (89.5%, V), Lespedeza maximowiczii (73.7%, IV), Isodon japonicus(73.7%, IV), Fraxinus rhynchophylla (68.%, IV), Arlemisia stolonifera (65, 8%, IV), etc. Artemisio-Quercetum mongolicae was chiefly distributed on the ridge lines of low altitudes (570~700m) and on the ground with exposed rocks in sloping surface (800~1, 000m). Both Fraxinus sievoldiana and Rhododendrou schlippenbachii of the Artemisio-Quercetum mongolicae showed a predominant condition all over the subtree and shrub layers. Of Artemisio-Quercetum pinetosum densifloraes, Pinus densiflora becomes powerful over the tree layer and, on the other hand, Quercus mongolica over the subtree layer. Lychno-Quercetum mongolicae was distributed somewhat on the humid region of the high altitude area (840~1.200m). Fraxinus rhynchophylla community was chiefly distributed in the vicinity of the ravines. Quereus rariabilis community was distributed at subxeric slope on southeast side of the mountain. Larix leptolepis has been reforested at the ecotone between Artemisio-Quercetum mongolicae and Lychno-Quercetum mongolicae. Percentage cover degrees of the subtree layer In Lychno-Quercetum mongolicae, Quereus rariabilis community and Larix leptolepis afforestation were relatively low, 15.3%, 5% and 2.5%, respectively.The contents of moisutre and organic matter in the soil were much higher in Luchno-Quercetum monogolicae and Larix leptolepis afforestaion than in the other communities. Contents o Ca, Mg, K and the available phosphorus were relaively high compared with forests of the other area. This result seems to be influenced by a forest fire. As the altitude increases, the moisure content in the forest soil also increases.

• Journal of Nutrition and Health
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• v.34 no.8
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• pp.896-904
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• 2001

Conjugated linoleic acid(CLA) is a group of positional and geometric isomers of linoleic acid(LA) and exhibits anticarcinogenic activity in multiple experimental animal models. Cis-9,trns-11(c9t11) and trans-10,cis-12(t10c12) CLA are the principal isomers found in foods. The present study was performed to determine whether CLA and the two isomers inhibits HT-29 cell proliferation and to assess whether such an effect was related to changes in secretion of eicosanoids. Cells were incubated in serum-free medium with various concentrations(0 to 20$\mu$M) of CLA or LA. CLA inhibited cell proliferation in a dose-dependent manner, with maximal inhibition(70 $\pm$ 1%) observed at 20$\mu$M concentration after 96 hours. However, LA had no effect at the same concentration range. To compare the ability of c9f11 and t10c12 to inhibit cell proliferation, cells were incubated with increasing concentrations(0 to 4$\mu$M) of these isomers. T10c12 inhibited cell proliferation in a dose-dependent manner. A 66 $\pm$ 2% decrease in cell number was observed within 96 hours after addition of 4$\mu$M t10c12. By contrast, c9t11 had no effect. The concentrations of CLA and the two isomers in the plasma membrane were increased when they were added to the incubation medium. However, they did not alter the levels of arachidonic acid in plasma membrane. To assess whether the proliferation inhibiting effect of CLA was related to changes in eicosanoid production, prostaglandin E$_2$(PGE$_2$) and leukotriene B$_4$(LTB$_4$) concentrations in conditioned media were estimated by a competitive enzyme immunoassay. Both CLA and t10c12 increased the production of materials reactive to PGE$_2$ and LTB$_4$ antibodies in a dose-dependent manner. By contrast, c9t11 had no effect. These results indicate that inhibition of HT-29 cell proliferation by CLA is attributed to the effect of the t10v12 isomer. The materials reactive to PGE$_2$ and LTB$_4$ antibodies may inhibit growth stimulatory effect of arachidonic acid-derived eicosanoids on HT-29 cell proliferation.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10513-10524
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• 2015

Background: Metastasis is a major reason for poor prognosis in patients with cancer, including hepatocellular carcinoma (HCC). A salient feature is the ability of cancer cells to colonize different organs. Long non-coding RNAs (lncRNAs) play important roles in numerous cellular processes, including metastasis. Materials and Methods: In this study, the lncRNA expression profiles of two HCC cell lines, one with high potential for metastasis to the lung (HCCLM3) and the other to lymph nodes (HCCLYM-H2) were assessed using the Arraystar Human LncRNA Array v2.0, which contains 33,045 lncRNAs and 30,215 mRNAs. Coding-non-coding gene co-expression (CNC) networks were constructed and gene set enrichment analysis (GSEA) was performed to identify lncRNAs with potential functions in organ-specific metastasis. Levels of two representative lncRNAs and one representative mRNA, RP5-1014O16.1, lincRNA-TSPAN8 and TSPAN8, were further detected in HCC cell lines with differing metastasis potential by qRT-PCR. Results: Using microarray data, we identified 1,482 lncRNAs and 1,629 mRNAs that were differentially expressed (${\geq}1.5$ fold-change) between the two HCC cell lines. The most upregulated lncRNAs in H2 were RP11-672F9.1, RP5-1014O16.1, and RP11-501G6.1, while the most downregulated ones were lincRNA-TSPAN8, lincRNA-CALCA, C14orf132, NCRNA00173, and CR613944. The most upregulated mRNAs in H2 were C15orf48, PSG2, and PSG8, while the most downregulated ones were CALCB, CD81, CD24, TSPAN8, and SOST. Among them, lincRNA-TSPAN8 and TSPAN8 were found highly expressed in high lung metastatic potential HCC cells, while lowly expressed in no or low lung metastatic potential HCC cells. RP5-1014O16.1 was highly expressed in high lymphatic metastatic potential HCC cell lines, while lowly expressed in no lymphatic metastatic potential HCC cell lines. Conclusions: We provide the first detailed description of lncRNA expression profiles related to organ-specific metastasis in HCC. We demonstrated that a large number of lncRNAs may play important roles in driving HCC cells to metastasize to different sites; these lncRNAs may provide novel molecular biomarkers and offer a new basis for combating metastasis in HCC cases.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.25 no.3
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• pp.1-8
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• 1980

Eight different weed community types growing in association with transplanted rice were identified in the experimental fields used in these studies. The Importance Value (LV.) of weed species growing in association with rice changed with time. The I. V. of Echinochloa glabrescens Munro ex Hook f. increased from 40 days after transplanting (DAT) until heading and then, decreased from heading to maturity of rice. However, the LV. of Monochoria vaginalis (Burm.) Pres!. and Echinochloa crus-galli ssp. hispidula (Retz.) Honda increased from 40 DAT to maturity while Scirpus maritimus L. decreased from 40 DAT. There was a high negative correlation between grain yield and weed weight at rice heading in all weed communities. The competitive ability of rice against weeds varied depending upon weed species. The highest yield decrease due to weed competition was obtained from the E. crus-galli ssp. hispidula-S. Maritimus community. Forty-two percent yield reduction was caused by competition of 100g dry weight of weeds per square meter of this community type at rice heading. The yield decrease for the same amount of weeds was 10% for the E. glabrescens community, 15% for the M vaginalis community and the M vaginalis-Scirpus supinus L. community and 21% for the M vaginalis-E. glabrescens-Fimbristylis littoralis Gaud.-S. maritimus community type.

• The Korean Journal of Nuclear Medicine
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• v.28 no.1
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• pp.22-30
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• 1994

Encephalo-duro-arterio-synangiosis (EDAS) is a relatively new surgical procedure for treatment of childhood moyamoya disease. We assessed regional cerebral perfusion in moyamoya patients before (1.3 mo) and after (6.8 mo) EDAS with $^{99m}Tc$-HMPAO brain SPECT. A total of 21 EDAS operations in 17 moyamoya patients was included. Preoperative CT or MRI showed cerebral infarction in 14 patients and carotid angiography showed Suzuki grade I to V stenosis in 6%, 9%, 62%, 12% and 12% of the hemispheres respectively. Preoperative SPECT showed regional hypoperfusion in all patients, bilateral frontal and temporal lobes being the most frequently involved site. $4{\times}4$ pixel sized ROIs were applied on the frontotemporal cortex in 3 slice averaged transverse tomographic images. An index of regional perfusion was measured as: PI (%)=average F-T activity/average cerebellar activity${\times}100$ Pre-EDAS ipsilateral PI ranged from 23.7 to 98.4% (mean: $74.3{\pm}17%$) and increased significantly after operation ($81.4{\pm}17%$, p<0.001). Individual post-EDAS PI improved in 15/21 cases, showed no significant change in 5 and was slightly aggravated in 1. The amount of clinical improvement (${\Delta}CI$) was graded with a scale of 0 to 4 based on frequency and severity of TIA attacks. When patients were grouped according to pre-EDAS PI, group II (PI 70-89) showed a significantly higher ${\Delta}CI$ (3.3) compared to group I (PI< 70, 1.57) or group III (PI >90, 0.5) (P< 0.001). The amount of perfusion improvement (${\Delta}PI$) showed significant correlation with ${\Delta}CI$ (r=0.42, p=0.04). ${\Delta}PI$ did not, however, correlate with the amount of neovascularization assessed angiographically in 8 patients. Serial HMPAO SPECT is an useful noninvasive study for assessing perfusion improvement after EDAS in childhood moyamoya patients.

• Journal of the Korean Society of Radiology
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• v.13 no.3
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• pp.473-479
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• 2019

PET-CT improves performance and reduces the time by combining PET and CT of spatial resolution, and uses CT scan for attenuation correction. This study analyzed PET image evaluation. The condition of the tube voltage and current of CT will be changed using. Uniformity phantom and resolution phantom were injected with 37 MBq $^{18}F$ (fluorine ; 511 keV, half life - 109.7 min), respectively. PET-CT (Biograph, siemens, US) was used to perform emission scan (30 min) and penetration scan. And then the collected image data were reconstructed in OSEM-3D. The same ROI was set on the image data with a analyzer (Vinci 2.54, Germany) and profile was used to analyze and compare spatial resolution and image quality through FWHM and SI. Analyzing profile with pre-defined ROI in each phantom, PET image was not influenced by the change of tube voltage or exposure dose. However, CT image was influenced by tube voltage, but not by exposure dose. When tube voltage was fixed and exposure dose changed, exposure dose changed too, increasing dose value. When exposure dose was fixed at 150 mA and tube voltage was varied, the result was 10.56, 24.6 and 35.61 mGy in each variables (in resolution phantom). In this study, attenuation image showed no significant difference when exposure dose was changed. However, when exposure dose increased, the amount of dose that patient absorbed increased too, which indicates that CT exposure dose should be decreased to minimum to lower the exposure dose that patient absorbs. Therefore future study needs to discuss the conditions that could minimize exposure dose that gets absorbed by patient during PET-CT scan.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.3
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• pp.474-480
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• 2004

The purpose of this in vitro study was to compare the remineralizing effects of three glass ionomer cements (high filled glass ionomer cement, compomer, resin modified glass ionomer cement) with resin composite (control group) on incipient interproximal caries, and to assess long-term change of remineralization effect, in each material, evaluated by microtomography. Proximal restoration was simulated with tooth specimen and Glass Ionomer Cements. And each of these groups was placed into a closed container with artificial saliva at $37^{\circ}C$ and pH 7.0 for a time period of thirty days with constant circulation. At the end of thirty and sixty days, tomographic images were taken from these specimens with micro CT scanner. Materials used in this study were as follows. Group 1: Fuji IX GP (GC Corp., Tokyo, Japan) Group 2: Vitremer (3M ESPE, St. Paul, Minn., USA) Group 3: F2000 (3M ESPE, St. Paul, Minn., USA) Group 4: Z250 (3M ESPE, St. Paul, Minn., USA) Using density-measuring program, the micro-density of carious lesions on the specimens were measured. The mean density changes of each group were compared to the other groups to evaluate the effect of remineralization. The results were as follows: 1. The lesion density of all groups increased. 2. The mean density increase of Group 1, 2, 3 were higher than that of Group 4 every month(p<0.05). 3. There were significant differences of density increase among glass ionomer group(Group 1, 2, 3).

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.3
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• pp.335-340
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• 2010

This study was conducted to select organic materials (OM) and nitrogen sources in composting of waste mushroom bed from Agaricus bisporus. We examined physio-chemical properties of the organic materials and the mixture ratio for preparing the wasted mushroom bed (M) compost. The carbon content of sawdust was higher than those of rice straw (R) as OM source and the nitrogen content was high in the order of fowl manure (F)>> pig manure (P)> cow manure (C). The compost was prepared to maintain the criteria of above 25% organic matter and then the change of their ingredients was estimated during the process of fermentation. The temperature of waste mushroom bed+pig manure+rice straw (MRP) treatment was varied fast throughout fermentation, on the other hand the temperature of waste mushroom bed+pig manure+sawdust (MSP) treatment was steadily elevated to the middle of composting. The pH of the compost was somewhat high to pH 8.5~9.0 at the early stage, but decreased to 7.5 at the end stage of composting. The content of OM after fermentation was decreased to the level of 19~21% in rice straw, but the sawdust treatment maintained 25~27% organic matter. The waste mushroom bed+fowl manure+rice straw (MRF) treatment, which contains 26.2% organic matter and 0.68% nitrogen, was the highest among them. The volume of compost was reduced to 50% by using rice straw as organic matter, but reduced to 30% by using the sawdust. The contents of heavy metal in the compost were suitable within the legal criteria. The number of microorganisms were higher in the rice straw than those in the sawdust. It was high in the order of fowl manure> pig manure> cow manure. The major groups consisted of aerobic bacteria, gram negative bacteria and Bacillus sp. and their populations after fermentation were increased to $1{\times}10^1{\sim}1{\times}10^2\;cfu\;g^{-1}$ rather than those before fermentation. Therefore we concluded that the waste mushroom bed+fowl manure+sawdust (MSF 3:9:1 v/v/v) treatment was suitable combination for high organic matter and nitrogen source, and the periods of composting were 50~60 days.