• Asian-Australasian Journal of Animal Sciences
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• v.26 no.6
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• pp.856-863
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• 2013

An in vitro gas production technique was used in this study to elucidate the effect of two strains of active live yeast on methane ($CH_4$) production in the large intestinal content of pigs to provide an insight to whether active live yeast could suppress $CH_4$ production in the hindgut of pigs. Treatments used in this study include blank (no substrate and no live yeast cells), control (no live yeast cells) and yeast (YST) supplementation groups (supplemented with live yeast cells, YST1 or YST2). The yeast cultures contained $1.8{\times}10^{10}$ cells per g, which were added at the rates of 0.2 mg and 0.4 mg per ml of the fermented inoculum. Large intestinal contents were collected from 2 Duroc${\times}$Landrace${\times}$Yorkshire pigs, mixed with a phosphate buffer (1:2), and incubated anaerobically at $39^{\circ}C$ for 24 h using 500 mg substrate (dry matter (DM) basis). Total gas and $CH_4$ production decreased (p<0.05) with supplementation of yeast. The methane production reduction potential (MRP) was calculated by assuming net methane concentration for the control as 100%. The MRP of yeast 2 was more than 25%. Compared with the control group, in vitro DM digestibility (IVDMD) and total volatile fatty acids (VFA) concentration increased (p<0.05) in 0.4 mg/ml YST1 and 0.2 mg/ml YST2 supplementation groups. Proportion of propionate, butyrate and valerate increased (p<0.05), but that of acetate decreased (p<0.05), which led to a decreased (p<0.05) acetate: propionate (A: P) ratio in the both YST2 treatments and the 0.4 mg/ml YST 1 supplementation groups. Hydrogen recovery decreased (p<0.05) with yeast supplementation. Quantity of methanogenic archaea per milliliter of inoculum decreased (p<0.05) with yeast supplementation after 24 h of incubation. Our results suggest that live yeast cells suppressed in vitro $CH_4$ production when inoculated into the large intestinal contents of pigs and shifted the fermentation pattern to favor propionate production together with an increased population of acetogenic bacteria, both of which serve as a competitive pathway for the available H2 resulting in the reduction of methanogenic archaea.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.2 no.3
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• pp.87-99
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• 1982

This study proposes a reliability based design criteria for the R.C. superstructures of highway bridges. Uncertainties associated with the resistance of T or rectangular sections are investigated, and a set of appropriate uncertainties associated with the bridge dead and traffic live loads are proposed by reflecting our level of practice. Major 2nd moment reliability analysis and design theories including both Cornell's MFOSM(Mean First Order 2nd Moment) Methods and Lind-Hasofer's AFOSM(Advanced First Order 2nd Moment) Methods are summarized and compared, and it has been found that Ellingwood's algorithm and an approximate log-normal type reliability formula are well suited for the proposed reliability study. A target reliability index (${\beta}_0=3.5$) is selected as an optimal value considering our practice based on the calibration with the current R.C. bridge design safety provisions. A set of load and resistance factors is derived by the proposed uncertainties and the methods corresponding to the target reliability. Furthermore, a set of nominal safety factors and allowable stresses are proposed for the current W.S.D. design provisions. It may be asserted that the proposed L.R.F.D. reliability based design criteria for the R.C. highway bridges may have to be incorporated into the current R.C. bridge design codes as a design provision corresponding to the U.S.D. provisions of the current R.C. design code.

• Composites Research
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• v.23 no.3
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• pp.19-30
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• 2010

The present study describes a method for analytical solution to elastic field in structural elements of general symmetric laminated composite materials. The two dimensional plane stress elasticity problems under mixed boundary conditions are reduced to the solution of a single fourth order partial differential equation, expressed in terms of a single unknown function, called displacement potential function. In addition, all the components of stress and displacement are expressed in terms of the same displacement potential function, which makes the method suitable for any boundary conditions. The method is applied to obtain analytical solutions to two particular problems of structural elements consisting of an angle-ply laminate and a cross-ply laminate, respectively. Some numerical results are presented for both the problems with reference to the glass/epoxy composite. The results are highly accurate and reliable as all the boundary conditions including those in the critical regions of supports and loads are satisfied exactly. This verifies the method as a simple and reliable one as well as capable to obtain exact analytical solution to elastic field in structural elements of composite materials under mixed and any other boundary conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.650-657
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• 2002

Two experiments were conducted to evaluate the effects of forage:concentrate ratios and feeding principles on milk yield, milk composition, body weight change, postpartum oestrus and feed cost. A total of 36 crossbred F1 cows (Holstein Friesian${\times}$Local Yellow) in the 8th week of lactation were used. In each experiment, animals were divided into three groups using a randomized block design according to the milk yield of the previous eight weeks. Cows were fed 30, 50 and 70% concentrate in the diet based on DM. In experiment 1 (Fc), cows were given the same amount of DM with constant ratios of forage and concentrate within treatments. In experiment 2 (Fa), cows were given the same constant amounts of concentrate as in experiment 1 and ad libitum forage. The forage consisted of a natural grass mixture based on 5 species of grasses with high nutritive values. There was no difference in total DM intake between treatments within experiments. However, cows fed forage ad libitum had higher DM intakes compared to cows fed constant forage (1.6, 4.5 and 9.5% for cows fed 70, 50 and 30% forage, respectively). Daily milk yield of cows fed forage ad libitum was higher than that of cows fed constant forage:concentrate ratio. Within experiment, milk yield was highest for cows fed 30% DM forage, followed by cows fed 50% and then cows fed 70% forage (11.17, 10.98 and 10.71 for the 30Fc, 50Fc and 70Fc cows; 11.73, 11.16 and 10.81 kg for the 30Fa, 50Fa and 70Fa cows, respectively). Decreased forage ratio in the diets resulted in decreased milk fat content and tended to increase milk protein. Increased concentrate ratio in the diet and feeding forage ad libitum increased body weight gain. The effect of forage:concentrate ratio on postpartum oestrus was not significant. The feed cost per kg milk produced was lowest for the cows fed 70% forage. It is concluded that increased ratio of concentrate resulted in increased body weight gain, milk yield, milk protein, and decreased milk fat. Feeding forage ad libitum increased feed intake, milk yield and body weight gain. The ratio of 50% forage is more suitable for milk production and animal condition, but in terms of feed cost and under the conditions of small dairy farmers, the 70% ad libitum forage feeding is recommended.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.7
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• pp.948-956
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• 2000

The effect of high and low level of feed intakes on nutrient digestibility, nutrient losses through methane, energy and protein utilization by goats fed on alfalfa (Medicago sativa L.) pellets based diets was investigated in this study. Twelve castrated Japanese goats were employed in two subsequent digestion and metabolism trials. The goats were divided into three groups, offered three diets. Diet 1 consisted of 100% alfalfa pellet, Diet 2 was 70% alfalfa pellet and 30% corn, and Diet 3 was 40% alfalfa pellet and 60% corn. The two intake levels were high (1.6 times) and low (0.9 times) the maintenance requirement of total digestible nutrients (TON). Rumen ammonia nitrogen ($NH_3$-N) level of Diet 1 was lower (p<0.001) compared to Diets 2 and 3, but the values were always above the critical level (I50 mg/liter), The pH values of rumen liquor ranged from 6.02 to 7.30. Apparent digestibility of nutrient components did not show differences (p>0.05) between the two intake levels but inclusion of corn significantly altered the nutrient digestibility. Diet 3 had highest (p<0.001) dry matter (DM), organic matter (OM), ether extract (EE) and nitrogen fee extract (NFE) digestibility followed by the Diet 2 and Diet 1. The crude protein (CP) digestibility values among the three diets were in a narrow range (70.1 to 70.8%). Crude fiber (CF) digestibility for Diet 3 was slight higher (p>0.05) than that for other two diets. When alfalfa was replaced by corn, there were highly significant (p<0.001) increases in DM, OM, EE and NFE apparent digestibility and a slight increase in the CF digestibility (p>0.05). There were no differences (p>0.05) in energy losses as methane ($CH_4$) and heat production among the diets but energy loss through urine was higher for the Diet 1. The total energy loss as $CH_4$ and heat production were higher for the high intake level but the energy loss as $CH_4$ per gram DM intake were same (0.305 kcal/g) between the high and low intake level. Retained energy (RE) was higher for Diet 3 and Diet 2. Nitrogen (N) losses through feces and urine were higher (p<0.001) for Diet 1. Consequently, N retention was lower (p>0.05) for Diet 1 and higher in Diets 3 and 2. It is concluded that inclusion of corn with alfalfa increased the metabolizable energy (ME) and RE, and retained N through reducing the energy and N losses. The high level of intake reduced the rate of nutrient losses through feces and urine.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.120-123
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• 2008

This experiment was conducted to investigate the effect of different nipple angles on water disappearance in growing pigs, which is defined as the water which leaves the watering device but is not consumed by the pig. This water adds to the volume of the total waste slurry. Four crossbred pigs averaging $70.0{\pm}1.4$ kg were assigned into 1 of 4 treatments using a $4{\times}4$ Latin-square design. Treatments were distinguished by the angle between nipple terminal and the perpendicular wall. These angles were: 1) NA-30 (= Nipple Angle $30^{\circ}$), 2) NA-45 (= Nipple Angle $45^{\circ}$), 3) NA-60 (= Nipple Angle $60^{\circ}$) and 4) NA-90 (= Nipple Angle $90^{\circ}$). All of the nipples were fixed at shoulder height of each pig. After a 7-day adaptation period, samples were collected from each pig for 4 days, followed by a day for change-over. Pigs were fed a 0.8 kg diet twice daily at 08:00 and 20:00 and supplied water ad libitum. Throughout the experimental period, pigs in the NA-30 treatment group showed greater water disappearance than other treatments. Water disappearance was the lowest for the NA-60 treatment group (p<0.01). The percentage of water disappearance to the water supply was significantly reduced in the NA-60 treatment group (p<0.01). Water intake was the same for all the treatment groups. The rate of water disappearance per unit of water intake was significantly decreased in pigs in the NA-60 treatment group compared to other groups (p<0.01). These results suggested that the nipple angle for growing pigs should be $60^{\circ}$ to reduce water disappearance and, subsequently, the amount of waste generated.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.9
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• pp.1278-1285
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• 2008

Measurement of gas produced during in vitro fermentation was used to investigate the fermentation characteristics and interactions of rice straw, wheat straw or maize stover mixed with alfalfa or clover at proportions of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Cumulative gas production was recorded at 2, 4, 8, 12, 16, 24 and 48 h of incubation, and the Gompertz function was used to describe the kinetics of gas production. In vitro dry matter and organic matter disappearances (IVDMD and IVOMD) were determined after 48 h incubation. The rate of gas production of clover was higher (p<0.05) than that of rice straw, wheat straw, maize stover and alfalfa when straws and hays were incubated separately. Increasing the proportion of alfalfa in the straw-alfalfa mixtures increased (p<0.05) the rates, but not the maximum volume of gas production. However, both rate and the maximum volume of gas production were increased (p<0.01) as the proportions of clover increased in the straw-clover mixtures. The cumulative gas production at 48 h, IVDMD and IVOMD showed no consistent interaction effects between different mixtures of cereal straws and hays. The extent of interactive effects was affected by the types of cereal straw, legume hay and their proportions in the mixture. The appropriate combination for the mixture of rice straw or maize stover with leguminous hays was 75:25 and 25:75, respectively. The better combination occurred at a proportion of 50:50 for the mixture of wheat straw and alfalfa. We conclude that the suitable proportion of low-quality straw and high quality legume hay combination should be considered in the ration formulation system of ruminants according to the extent of positive interactive effects.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.1
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• pp.1-8
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• 1991

Population dynamics of Matted sole Limanda yokohamae($G{\"{U}}NTHER$) in Tokyo bay, Japan has been studied by virtual population analysis (VPA) for multi cohort and experimental fishing. Based on the biological data, the present parameters of the Limanda yekohamae stock at the Tokyo bay, Japan were estimated as follows: natural mortality coefficient(M) were 0.313 for male and 0.250 for female, terminal fishing mortality coefficient(F) were 2.190 for male, and 0.798 for female, rate of exploitation(E) was $30\%\;to\;50\%$. From the result of virtual population analysis for multi cohort, the population size were estimated from 3,5000,000 to 9,200,000 fishes, according to the result of experimental fishing, estimated stock size were 2,400,000 to 8,700,000 fishes. Stock size difference of the two methods were about two times in 1987, however, other years has been showed from 0.8 to 1.5 times. Both method has been showed same increase and decrease tendency of the c. p. u. e. and catches. From the isopleth diagram plot by Beverton and Holt's yield per recruit, the catches could be increase two times for female, 1.3 times for male than present aspects by the fishing management. And further, as reducing fishing effort, extension of mesh size and rising the length at first caputre, are reasonable in order to manage the stock at the optimum level.

• Journal of Nutrition and Health
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• v.19 no.4
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• pp.246-254
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• 1986

Weanling female Sprague Dawley rats were fed d diets containing 22mg pyridoxine. BCI/kg diet (control diet) and l.2mg pyridoxine. BCI/kg diet (deficient diet). One control group and one defi­c dent group were fed their diet throughout growth, g gestation and lactation. After the pups were born and weaned, the deficient group was divided into two groups. One switched to control diet(supple­I mented group) and the other continued the same d deficient diet( deficient group) until 10 week -old. The liver mitochondrial and cytosolic asparate a aminotransferase activity and pyridoxal phosphate content were determined in offspring rats. The aspartate aminotransferase activities in both liver mito$\phi$ondrial and cytosolic fractions of den­d cient group were significantly lower than those of controls, but there were no significant differences between two groups after addition of 1O^{-4}M pyri­d do뼈I phosphate to the medium. By pyridoxine s supplementation after weaning, the reduced aspar­a tate aminotnmsferase activities were only partialy I restored to control levels. The pyridoxal phospha­t te content of deficient group in Iiver mitochondr­ial and cytosoIic fractions were alo significantly different from those of controls, but readily restored by dietary supplementation. These results suggest that there is a quantitative and a qualitative changes of aspartate amino trans­f ferase and pyridoxal phosphate in liver mitochon­d drial and cytosolic fraction by long-term pyrido­x xine deficiency and these reductions can partially recovered by dietary pyridoxine supplementation after weaning.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.7
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• pp.942-948
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• 2005

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a non-synonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variable-region-like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.