• Title/Summary/Keyword: Exudates

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Studies on the Technical Development of Traditional Korean Golden Varnish (Hwangchil)(II) - Chemical Composition and Coatings-oil Characteristics of the Exudates and Bark-extractives of Hwangchil-namu(Dendrogapax morbifera Lev.) - (전통 황칠 도료 개발에 관한 연구 (II) - 황칠나무 수피 추출물 및 삼출액의 화학적 조성과 도료용 오일특성 -)

  • Lim, Kie-Pye;Jung, Woo-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.26 no.1
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    • pp.7-13
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    • 1998
  • In order to reconstruct the traditional technology of Korean golden varnish(Hwangchil) made from the exudates of D. morbifera, this study in the second step was carried out to analyze the chemical composition and oil properties of the exudates for coatings and bark extractives made from the trees of D. morbifera more than 20 years old grown in Wando and Jejudo islands in the southwestern part of Korean peninsula according to the physiological seasons. The results obtained are as follows: 1. The exudates appeared to be a phytoalexin because it exudated after several days in tapping only in summer season. 2. Both the exudates and bark-extracts with acetone contained 50~60% unsaponifiables and 30~50% saponifiables 3. A major component of essential oil in bark-extractives was ${\beta}$-cubebene and reached to 60~80%, while that of exudates included only 34% ${\beta}$-cubebene and some other compounds such as ${\beta}$-cadinene of 12%, aromadendrene of 9%, ${\beta}$-selinene of 9%, CAS030021-74-0 of 10%, etc. in GC-MS spectrometer. 4. A mjor component of fatty oil fraction in bark-extractives was linoleic acid and reached to more than 60%, while the exudates had mostly other components such as terpenes and phenolics instead of fatty acids accordint to GC-MS spectrometer. 5. Iodine value of samples after oil refining had 214mg/g in the exudates and more than than 150mg/g in bark-extractives, so the latter belonged to a drying oil.. 6. Therefore, the exudates from D. morbifera for traditional Korean golden varnish seems to have beeen used to a good varnish because it has some specific compounds different from its bark-extractives or general varnishes.

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A Diagnostic Significance of Pleural Fluid Cholesterol Level (흉막액 Cholesterol 농도에 대한 진단적 의의)

  • Kim, Young-Kyoon;Kwon, Soon-Seog;Kim, Kwan-Hyoung;Han, Ki-Don;Moon, Hwa-Sik;Song, Jeong-Sup;Park, Sung-Hak
    • Tuberculosis and Respiratory Diseases
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    • v.38 no.3
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    • pp.262-269
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    • 1991
  • The purpose of this study is to investigate the utility of the pleural fluid cholesterol level in separating the exudates from the transudates, and in differentiating tuberculous exudates from non-tuberculous exudates, 52 patients with pleural effusion were involved in this prospective study. By predefined criteria, 40 of these effusions were classified as exudates (Group I) and 12 as transudates (Group II). Group I was subdivided into tuberculous exudates (Group A) and non-tuberculous exudates (Group B). The followings are parameters used in separating the exudates from the transudates; pleural protein (P-PROT) 3.0 g/dl, pleural protein/serum protein ratio (P/S PORT) 0.5, pleural LDH (P-LDH) 200 IU, pleural LDH/serum LDH ratio (P/S LDH) 0.6, pleural cholesterol (P-CHOL) 50 mg/dl and pleural cholesterol/serum cholesterol ratio (P/S CHOL) 0.4. Mean values of the parameters in each group were compared, and then misclassified rate and the dignostic efficiency for each parameter were calculated. The results were as follows; 1) Mean P-CHOL ($94.98{\pm}73.86\;mg/dl$) in Group I was higher than that ($36.5{\pm}26.5\;mg/dl$) in Group II (p<0.05), but there was no significant difference in mean P-CHOL between Group A and Group B. 2) Mean P/S CHOL ($0.64{\pm}0.39$) in Group I was also higher than that ($0.27{\pm}0.15$) in Group II (p<0.01), but no difference was observed in mean P/S CHOL between Group A and Group B. 3) Misclassified rates for each parameter in separating the exudates from the transudates were as follows; P-PROT 1.9%. P/S PROT 3.8%. P-CHOL 9.6%, P/S CHOL 11.5%, P/S LDH 11.5%, and P-LDH 17.3%. 4) Diagnostic efficiencies for each parameter in separating the exudates from the transudates were as follows; P-PROT 98.1%, P/S PROT 96.2%. P-CHOL 90.4%. P/S CHOL 88.5%, P/S LDH 88.5%, and P-LDH 82.7%. In conclusion, we think that the pleural fluid chloesterol level could be used as a supportive parameter in separating the exudates from the transudates, but could not be used as a parameter in differentiating tuberculous exudates from non-tuberculous exudates.

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Role of Silver-containing Carboxymethyl Cellulose Dressing in the Management of Exudative Infected Wounds (삼출액과 감염이 동반된 창상의 치료에 있어 은 함유 카르복시메틸 셀룰로즈 드레싱의 역할)

  • Lee, Ji Hyuck;Lee, Eun Sang;Kang, So Ra
    • Archives of Plastic Surgery
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    • v.34 no.3
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    • pp.305-313
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    • 2007
  • Purpose: Wound healing is a result of complex processes whose components, such as cells, extracellular matrix, proteolytic enzymes, and their inhibitors receive effects from immune compartments, cytokines, chemokines, and growth factors. Impairment of normal physiologic response to wounding makes nonhealing chronic wounds. Wound infection and exacerbated proteolytic process may induce uncontrolled tissue degradation or exudates formation, which may result in the development of a nonhealing chronic wound. Thus proper management of wound infection and exudates is critical to prevent and treat nonhealing wound. The aim of this study is to evaluate effects of Aquacel AG, silver-containing carboxymethylcellulose dressing on treatment for exudative infected wound. Methods: The study included 31 patients with nonhealing wound. Wound was dressed with Aquacel AG. The effect of dressing was investigated by serial bacterial culture and wound exudates assessment. Each infection and exudates control time was determined and statistically analyzed. Results: Wound infection and exudates were effectively managed using Aquacel AG dressing. Mean infection and exudates control time were $3.4{\pm}1.2$ and $5.7{\pm}1.4$ weeks, respectively. Statistical analysis of the data indicated that infection control time correlated positively to age and exudates control time (p<0.05). Conclusion: There is as yet no ideal dressing for the topical treatment of chronic nonhealing wound. But silver-containing carboxymethylcellulose dressing can be used effectively for exudative, infected nonhealing wound.

Studies on the Development of Traditional Korean Golden Varnish(Hwangchil)(I) - Anatomical Characterisitics and Chemical Composition of Wood and Exudates of Hwangchil-namu(Dendropanax morbifera)- (전통 황칠 도료 개발에 관한 연구(I) - 황칠나무와 황칠액의 해부학적 특성과 화학적 조성-)

  • Lim, Kie-Pyo;Kim, Yoon-Soo;Chung, Woo-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.25 no.3
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    • pp.24-28
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    • 1997
  • As a way to revive the traditional technology of Korean golden varnish(Hwangchill), the exudates of D. morbifera, this study was carried out at first to investigate the anatomical characteristics and the chemical composition of D. morbifera stems and their exudates. Trees of more than 20 years old were harvested at Wando and Jejudo islands in the southwestern part of Korean peninsula during different physiological seasons of winter, spring, and summer, The results obtained are as follows: 1. In the anatomical aspect of wood, Hwangchil woods is ring-porous wood, has alternate inter-vessel pittings and horizontal intercellular canal in xylem. 2. In the chemical aspect of wood and bark, the general compositions appeared hot to differ from those of other hardwoods, and ash and alcohol-benzene extractives showed little increase with the increase of atmospheric temperature of harvesting season. 3. In the solvent-sequential extraction of bark, wood and exudate, the exudates was extracted up to 80% by ether, but the bark and wood contained a very small amount of ether extractives, about 2% in the bark and 0.5% in the xylem.

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Relationship Between Susceptibility to Pythium Seedling Blight and Pea Seed Exudates (완두종자분필물과 Pythium에 의한 묘립고병에 대한 감수성과의 관계)

  • Ohh Seung Hwan
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.193-197
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    • 1977
  • Seeds of pea cultivars susceptible to damping-off caused by Pythium ultimum produced sugars intheir exudates in half the time required for resistant cultivars. Also, the amount of reducing sugars was much greater in seed exudates of susceptible than resistant cultivars. Raffinose, melibiose, sucrose, glucose, and fructose (but not maltose, xylose or ribose) were identified in both resistant and susceptible cultivars; however, more glucose and sucrose were detected in seeds from susceptible than from resistant cultivars. Pythium ultimum grew more profusely around seeds from, and in exudates of, susceptible than resistant cultivars.

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Anthocyanins, Polyphenols and Antioxidant Activities of Black Raspberry Exudates (Black Raspberry 침출액의 Anthocyanin 및 Polyphenol 함량과 항산화능)

  • Lee, Bo-Kyung;Shin, Hye-Hyun;Jung, Ji-Hyun;Hwang, Keum-Taek;Lee, Yeon-Sook;Kim, Tae-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.2
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    • pp.125-130
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    • 2009
  • Intact black raspberry fruits, which were crushed by hand or ground using a blender, were immersed in 20%, 40%, 60%, and 80% ethanol (125 g fruits per 900 mL ethanol) and exuded for 30, 60, and 90 days at room temperature. Monomeric anthocyanin contents (MAC) and polyphenol contents (PHC) of the exudates were determined measuring absorbance using a spectrophotometer. DPPH and ABTS free radical scavenging activities (50%: $SC_{50}$) were determined for the antioxidant activities of the exudates. Twenty % ethanol exudates from black raspberry had significantly lower MAC and PHC regardless of the fruit types. $SC_{50}$ were higher in 20% ethanol exudates, suggesting 20% ethanol exudates from black raspberry had lower antioxidant activities. MAC and PHC of the black raspberry exudates decreased with time. This study suggests that MAC and PHC in the black raspberry could be exuded more efficiently using 40% or higher concentration of ethanol and that crushing and grinding little affect exuding MAC and PHC. The exudates with higher MAC and PHC showed higher antioxidant activities.

Chemotactic Response Study of Cylindrocarpon destructans towards Ginseng Root Exudates (인삼 추출물에 의한 Cylindrocarpon destructans의 주화성 반응 연구)

  • Xu, Yonghua;Chi, Kun;Zhang, Aihua;Lei, Fengjie;Yang, He;Zhao, Yan;Li, Kuo;Wang, Erhuan;Li, Qiong;Kim, Jong Seog;Lee, Seung Ho;Kim, Young Chang
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.5
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    • pp.360-369
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    • 2016
  • Background: Cylindrocarpon destructans (Zins) Scholten is an important pathogenic fungus that causes ginseng root rot in many ginseng growing areas in China. Although C. destructans have been studied worldwide, research on its chemotaxis towards ginseng (Panax ginseng C. A. Meyer) root exudates in the rhizosphere remains limited. Methods and Results: In this study, we collected ginseng root exudates with three different polarities from three-year-old ginseng roots, and performed chemotaxis and spore germination assays to investigate the ability of these exudates to induce the response in C. destructans. The results showed that, compared with other conditions, when C. destructans cultivated at $20^{\circ}C$ and a pH of 6 exhibited a strong positive chemotactic response toward $2mg/{\ell}$ aqueous phase, $20mg/{\ell}$ butanol phase, and $0.2mg/{\ell}$ petroleum ether from ginseng root exudates, the chemotactic moving indexes were 0.1581, 0.1638 and 0.1441, respectively. In addition, the spore germination rate with optimal chemotactic parameters were 48%, 53%, and 41% in the aqueous phase, butanol phase and petroleum ether groups, respectiviely, which were significantly higher than that in the control group (23%) (p < 0.05). The mycelial growth rate with optimal chemotactic parameters increased with culture time, and the maximum growth rates in the aqueous phase, butanol phase and petroleum ether groups were 0.425, 0.406 and 0.364 respectively, on the 4th day. The optimal chemotactic parameters were $39.73mg/50mg/{\ell}$, $48.93mg/50mg/{\ell}$, and $31.43mg/50mg/{\ell}$, in aqueous phase, butanol phase and petroleum ether respectively, from ginseng root exudates, compared with $5.5mg/50mg/{\ell}$, in the control group. Conclusions: The present study revealed that certain ginseng root exudates containing chemical attractants act as nutritional sources or signals for C. destructans and support its colonization of ginseng roots.

Leaf Exudates of Vicia faba and their Effects on Botrytis fabae and Some Associated Fungi

  • Migahed, Fatma F.;Nofel, Ashraf M.
    • Mycobiology
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    • v.29 no.4
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    • pp.198-204
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    • 2001
  • Analysis of leaf exudates of Vicia faba using paper chromatography to identify individual amino acids and sugars qualitatively was investigated. The results revealed that the number of identified amino acids detected in the leaf exudates of the susceptible plants was more than those of resistant plants. The results also showed an increase in the number of amino acids exuded by infected leaves, but no marked difference in sugars of infected and non infected plants. Lithium chloride application led to decrease in amino acid and sugar contents. The number of amino acids and sugars was also decreased with leaf age. Botrytis fabae and the selected fungal species(Alternaria alternata, Fusarium oxysporum and Aspergillus niger) were used to show the effect of individual amino acid and sugar on their spore germination. It was observed that all amino acids stimulated the fungal spore germination except serine which inhibited its spore germination. In case of A. alternata, spore germination was stimulated by all amino acids except serine, alanine, glutamic acid, arginine and methionine which caused the inhibition. In case of F. oxysporum, aspartic and glutamic acids inhibited spore germination but the other amino acids stimulated its spore germination. Aspartic acid and phenyl alanine inhibited the spore germination of A. niger. All the identified sugars(galactose, glucose, fructose and rhamnose) stimulated spore germination of all tested fungi.

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Studies on the Technical Development of the Traditional Korean Golden Varnish(Hwangchil) (III) - Main Component Analysis of Korea Golden Varnishes Traditonally Refined from the Exudates of Dendropanax morbifera Lev. - (전통 황칠 도료 개발에 관한 연구(III) - 전통 황칠 도료의 주성분 분석 -)

  • Lim, Kie-Pye;Jung, Woo-Yang;Hong, Dong-Hwa
    • Journal of the Korean Wood Science and Technology
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    • v.26 no.3
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    • pp.73-80
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    • 1998
  • In order to reconstruct the traditional technology of Korean golden varnish coatings, this study was carried out to separate and determine some main coloring components of the exudates of D. morbifera and its traditionally refined golden varnishes using a process of solvent extractions, chromatographies and spectrometries. The results obtained are as follows: 1. The exudate and its traditional-refined golden varnishes appear to have a kind of natural polyacetylenes because it has some triple bond peaks in FT-IR spectrometry. 2. Some yellowing spots of the polar-solvent extrats from the exudates and refined varnishes separated on TLC appeared under natural drying condition, but those of non-polar solvent extract such as hexane did not. 3. A traditional refining method for reconstructing a Korea golden varnishes was thought to be better than solvent separation because the former had higher triple-bond peaks than the latter in FT-IR spectrometry. 4. One of main conponents in the hexane-extracts of the traditional-refined varnishes and the exudates had the same molcular weighr of 204, but the fragmentation patterns was a little different between the exudate and the refined. in LC-MS soectrometry.

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Histopathological Studies on Enzootic Pasteurellosis of Domestic Rabbits in Korea (가토(家兎)의 Pasteurella성폐염(性肺炎) (Snuffles)에 관(關)한 병리학적(病理學的) 검색(檢索))

  • Kwon, Young Bang;Lim, Chang Hyeong
    • Korean Journal of Veterinary Research
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    • v.8 no.1
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    • pp.18-23
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    • 1968
  • Pathological studies on the Enzootic Pasteurellosis of domestic rabbits were performed and discussed. The chief pathological findings were as follows: Grossly, pneumonic lesions were located mainly in apical and cardiac lobes. The lesions were well demarcated with unaffected surrounding tissue. The pleural surface of pneumonic lesions were usually appeared as yellowish~white fibrinous exudate and, in some cases, abscesses were also shown. Microscopically, various cellular exudates composed mainly of neutrophils, large monocytes and lymphocytes were observed in the alveolar spaces. Some alveolar spaces were filled mostly with fibrinous exudates. Occasionally, thrombosis accompanied with infarcts were encountered in pneumonic lesions. There were deposition of purulo-fibrinous exudates on the visceral pleura.

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