• Title/Summary/Keyword: Extraction treatment

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Enzymatic Extraction of Pilocarpine from Pilocarpus jaborandi (Pilocarpus jaborandi로부터 필로카르핀의 효소반응추출)

  • Cho, Jun-Ho;Bhattarai, Saurabh;Oh, Tae-Jin;Jang, Jong Hwa
    • Microbiology and Biotechnology Letters
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    • v.41 no.2
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    • pp.236-241
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    • 2013
  • Pilocarpine is an imidazole alkaloid, found exclusively in the Pilocarpus genus, with huge pharmaceutical importance. In order to extract pilocarpine from Pilocarpus jaborandi, environmentally friendly enzyme-assisted extraction was applied. Viscozyme$^{(R)}$ L, a commercially available enzyme cocktail, was used for the study. The conditions for extraction were optimized on the basis of substrates, enzymes, temperatures and pHs. Optimum conditions for extraction with the highest yield were 30 h reaction of 100 mg substance at $45^{\circ}C$ in 40 ml of 50 mM acetic acid, pH 4. A 10% enzyme concentration was found to be the best for extraction. Total pilocarpine content after extraction was analyzed by HPLC. The total pilocarpine content ($1.14{\mu}g/mg$) obtained from Viscozyme$^{(R)}$ L treatment was 3.08-fold greater than those of the control treatment ($0.37{\mu}g/mg$).

Short-Term Surgical Outcome of the Partial Nail Extraction in Ingrown Nail of Military Trainee: Is Matrixectomy Necessary? (훈련병에게 처음 발생한 내향성발톱에서 부분 발톱 절제술의 단기 추시 결과: 기질 절제술이 꼭 필요한가?)

  • Kim, Jaeyoung;Kim, Yoon Seok;Yi, Young
    • Journal of Korean Foot and Ankle Society
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    • v.23 no.2
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    • pp.52-57
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    • 2019
  • Purpose: An ingrown nail is common in military trainees who are exposed to highly demanding activities. Although the matrixectomy procedure has been the main treatment modality, several drawbacks may follow after the procedure, such as infection, periostitis, and continued pain that causes a delayed return to duty. This study examined the outcomes of a simple partial nail extraction with the hypothesis that this procedure may bring an earlier return to duty, lower the perioperative complications, and produce a comparable recurrence rate. Materials and Methods: The medical records of patients who had surgical treatment for an ingrown nail in the authors' institution between April 2016 and December 2017 were reviewed retrospectively. Under the inclusion and exclusion criteria, 28 patients with a simple partial nail extraction (group A) and 29 patients with a partial nail extraction with matrixectomy (group B) were investigated. As the clinical outcome, the visual analogue scale (VAS) and satisfaction score, time to return to duty, complications, and recurrence rate were checked and compared between the groups. Results: The VAS scores of group B were significantly higher during the first (p<0.001) and second (p=0.026) follow-up week than group A. The time to return to duty was shorter in group A (7.8 days) than group B (10.1 days), and this difference was significant (p<0.001). Group B had five patients with complications, whereas group A had none (p=0.028). No differences in the recurrence rate (p=0.197) and patient satisfaction (p=0.764) were found between the groups. Conclusion: In this study, simple partial nail extraction in military trainees resulted in lower postoperative pain, lower complication rates, and earlier return to duty than the procedure with matrixectomy. Military trainees are temporarily exposed to highly demanding activities. Thus, a satisfactory outcome would be expected with simple partial nail extraction without performing a radical procedure, such as a matrixectomy.

Compromised extraction sockets: a new classification and prevalence involving both soft and hard tissue loss

  • Kim, Jung-Ju;Amara, Heithem Ben;Chung, Inna;Koo, Ki-Tae
    • Journal of Periodontal and Implant Science
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    • v.51 no.2
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    • pp.100-113
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    • 2021
  • Purpose: Previous studies have solely focused on fresh extraction sockets, whereas in clinical settings, alveolar sockets are commonly associated with chronic inflammation. Because the extent of tissue destruction varies depending on the origin and the severity of inflammation, infected alveolar sockets may display various configurations of their remaining soft and hard tissues following tooth extraction. The aim of this study was to classify infected alveolar sockets and to provide the appropriate treatment approaches. Methods: A proposed classification of extraction sockets with chronic inflammation was developed based upon the morphology of the bone defect and soft tissue at the time of tooth extraction. The prevalence of each type of the suggested classification was determined retrospectively in a cohort of patients who underwent, between 2011 and 2015, immediate bone grafting procedures (ridge preservation/augmentation) after tooth extractions at Seoul National University Dental Hospital. Results: The extraction sockets were classified into 5 types: type I, type II, type III, type IV (A & B), and type V. In this system, the severity of bone and soft tissue breakdown increases from type I to type V, while the reconstruction potential and treatment predictability decrease according to the same sequence of socket types. The retrospective screening of the included extraction sites revealed that most of the sockets assigned to ridge preservation displayed features of type IV (86.87%). Conclusions: The present article classified different types of commonly observed infected sockets based on diverse levels of ridge destruction. Type IV sockets, featuring an advanced breakdown of alveolar bone, appear to be more frequent than the other socket types.

Analysis of Some Online Questions with High Frequency about Dental Treatment in Korea

  • Kang, A-Reum;Go, Ye-Eun;Kim, Ka-Eun;Kim, Min-Joo;Kim, Seon-Jeong;Hwang, SooJeong
    • Journal of dental hygiene science
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    • v.19 no.3
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    • pp.190-197
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    • 2019
  • Background: The Internet has advantages in terms of accessibility and amount of information, and the search for health information over the Internet is increasing exponentially. The purpose of this study is to analyze the information generated about some dental treatment on the internet by year. Methods: Naver Knowledge (JisikIn in Korean) which is an interactive search service was selected as the first search site in Korea. Scaling, wisdom tooth extraction, and endodontic treatment that can be paid by Korean health insurance were selected. Finally, 4,729 questions about scaling, 23,963 wisdom teeth extraction questions and 17,733 endodontic treatment questions were extracted. The question contents, the information about the questioner and the answerer, and an error of answers were investigated. Frequency analysis was used and chi-square test was used if necessary. Results: The most frequently asked questions were discomfort and dissatisfaction after the treatment. The need for treatment was the second in questions of the wisdom tooth extraction and endodontic treatment, but the health insurance benefit was the second in dental scaling. Most of the questioners didn't disclose personal information. The public answered the most in 2013~2014, but the highest percentage of the respondents was experts in 2017. Responses were mostly personal experience, but showed a tendency to decrease with years, and professional knowledge showed an increasing tendency. The error of the answer has also gradually decreased. Conclusion: Questions about dental care over the Internet are increasing exponentially, experts are responding increasingly, and errors in answers are decreasing. Nevertheless, it is necessary to pay attention to the related expert group to prevent misinformation.

The Study of Curcuma Longs L. Dyeing (울금의 염색성에 관한 연구)

  • 주영주;소황옥
    • Journal of the Korean Society of Clothing and Textiles
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    • v.20 no.3
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    • pp.429-437
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    • 1996
  • This paper surveys extraction condition-temperature, pH, and changes of adsorbance in accordance with extraction condition of curcuma Longa L. solution. Using extracted curcuma Longa L. Solution, dyeing behaviors with natural fiber, effect of mordants to color fastness and color change were investigated. It was found that the amount of absorption (K/S value) was increased by mordants (Fe, Cr) and mordants treatment affected color change of dyed fabric. Among the mordants, effect of citric acid to color change of dyed fabric was the smallest, and color difference of post- mordant treatment is smaller than that of pee-mordant treatment in making use of citric acid as mordant. And K/S value of post mordant was higher than that of pre mordant. It was found that the pre - and post- mordants treatment little affected color fastness, but affected the amound of adsorption and color change of dyed fabric.

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Development of Separating Techniques on Quercetin-Related Substances in Onion(Allium cepa L.) 2. Optimal Extracting Condition of quercertin-Related Substances in Onion (양파의 Quercetin 관련 물질의 분리 기술 개발 2. 양파의 Quercetin 물질의 최적 추출조건)

  • 강성구;김용두;현규환;김영환;서재신;박양균
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.4
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    • pp.687-692
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    • 1998
  • To use onion(Allium cepa L.) residue as raw materials of food product, yields of onion-juice according to various extracting methods and optimal extracting condition of quercetin and its related glycosides were carried out. Onion juices gained by the methods of pressing, rotary crushing, freeze pressing and enzyme treatment. The yield by the method of enzyme treatment was higher than others. The yields of juice from fresh onion and heat-treated onion(8$0^{\circ}C$/10min) by pressing were 66% and 83%, respectively. Ethanol extraction of onion was efficient at 75$^{\circ}C$ and acetic acid extraction was proper at 3% concentration for 3 hours at 11$0^{\circ}C$. The onion extract was fractionated in the order of hexane, chloroform, ethylacetate and butanol to test flavonoids. The highest abundant flavonoids were found in ethylacetate and butanol fraction.

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Changes in Extraction Efficiency of Pine Needles depending on Extraction Method and the Condition (추출 방법과 조건에 따른 소나무 지엽 추출효율 변화)

  • Kim, Dong Sung;Kim, Hyung Min;Sung, Yong Joo;Kang, Seog Goo;Kang, Ho-Yang;Lee, Jun-Woo;Kim, Se Bin;Park, Gwan-Soo
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.48 no.1
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    • pp.93-99
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    • 2016
  • The extraction efficiency depending on the extracting methods and the conditions of extraction was investigated. The common steam extraction was compared to the distillation extraction method. The effects of the samples size and the extraction time on the extract yield were also investigated by using UV-Vis spectrophotometer. One of the functional components of pine needle extract as the natural phenol base components were detected by the UV-VIS at around 235 nm wavelength range. The absorbance intensity at around 235 nm wavelength of the pine needle extract was used as the indicator of the extraction efficiency in this experiment. The distillation extraction showed the higher extract yield than the steam extraction. The grinding treatment of pine needles also resulted in the better extract performance, but the severe grinding showed a little decrease in the extract yield especially in case of the distillation extraction method. More than half of the extract was collected at the first stage of the extraction, that was the first 15 minutes in the total 60 minutes extraction.

Evaluation of the Extraction Process in the Recycling of the Biological Waste (폐생물자원 활용에서 추출공정의 특성 평가)

  • Sung, Yong-Joo;Han, Young-Lim;Kim, Kun-Soo
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.38 no.3 s.116
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    • pp.72-78
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    • 2006
  • The extraction properties of the biological waste originated from the Tobacco industry were evaluated. The biological waste have been recycled and transformed into a valuable material, so called a reconstituted tobacco sheet(Recon) by the papermaking process. In this process, The mechanical extraction process, which divides the mixed raw material slurry into the soluble stock and the insoluble stock, could affect not only the quality of final Recon product but also the productivity of whole Recon making process. This study investigated the effects of the extraction process on the slurry properties in detail. In order to quantify the amounts of the solubles which resided in the insoluble fibers, the washing treatment of the stock before and after squeezing process was applied. The amounts of the residual solubles showed little changes according to each stage of the extraction process. The fractionation of the slurry showed the difference in the amount of soluble in the stock depending on the size of the biological waste. After the extraction process, the bigger size fiber portion contained about 19%(by weight) in soluble after pressing but the smaller size fiber portion 9% in soluble. The fractionation ratio of the stocks also was changed by the screw press process, which could demonstrate the physical effects of the mechanical extraction.