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Effects of Bovine Serum Albumin and Sugars on Sperm Livability and Acrosome Morphology of Frozen-thawed Boar Semen (소혈청알부민과 당류가 돼지 동결정자의 생존성 및 두모형태에 미치는 영향)

  • 윤종택;임경순;이용빈
    • Korean Journal of Animal Reproduction
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    • v.10 no.1
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    • pp.19-26
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    • 1986
  • This experiment was carried out to investigate the effect of bovine serum ablumin (BSA), sugars, glycerol equilibration time, straw size and thawing method on the survival index and the morphology of frozen boar spermatozoa. The results obtained were summarized as follow: 1. When the semen frozen in BF5 dilutor as pellet form was thawed in BTS at 37$^{\circ}$and 50$^{\circ}C$, BF5 dilutor with fructose showed higher sperm survival index than that with dextrose, however, when the semen was thawed on dry test tube at 37$^{\circ}C$, BF5 dilutor with sucrose showed higher sperm survival index than with other sugars. 2 When the semen forzen in BF5 dilutor with straw and thawed at 37$^{\circ}C$, BF5 dilutor with dextrose showed higher sperm survival index than those with other sugars, and there was no difference in sperm survival index between 0.5 and 1.0 ml straws. 3. The sperm survival index of frozen sperm was significantly (P<0.05) improved due to addition of BSA (0.05%) to BF5 dilutor. 4. When the extended semen with BF5 dilutor contatining 0.01 to 0.05% of BSA was frozen in the straw, the semen without glycerol equilibration showed significantly (P<0.05) higher sperm survival index than those with 2, 4 and 6 hrs glycerol equilibration time. 5. The sperm frozen in BF5 dilutor with dextrose or fructose, sucrose and raffinose showed 77 to 88% in normal acrosome rate and no difference among sugars. 6. The frozen semen showed lower normal acrosome rate than the first and second diluted semen, whereas the frozen semen showed higher swollen, damaged and missing acrosome rate than the first and second diluted semen. 7. Damaged and missing acrosome rate of sperm head due to freezing was somewhat inhibited by addition of BSA (0.01 to 0.05) to the BF5 dilutor.

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A component method model for blind-bolts with headed anchors in tension

  • Pitrakkos, Theodoros;Tizani, Walid
    • Steel and Composite Structures
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    • v.18 no.5
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    • pp.1305-1330
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    • 2015
  • The successful application of the component-based approach - widely used to model structural joints - requires knowledge of the mechanical properties of the constitutive joint components, including an appropriate assembly procedure to derive the joint properties. This paper presents a component-method model for a structural joint component that is located in the tension zone of blind-bolted connections to concrete-filled tubular steel profiles. The model relates to the response of blind-bolts with headed anchors under monotonic loading, and the blind-bolt is termed the "Extended Hollo-bolt". Experimental data is used to develop the model, with the data being collected in a manner such that constitutive models were characterised for the principal elements which contribute to the global deformability of the connector. The model, based on a system of spring elements, incorporates pre-load and deformation from various parts of the blind-bolt: (i) the internal bolt elongation; (ii) the connector's expanding sleeves element; and (iii) the connector's mechanical anchorage element. The characteristics of these elements are determined on the basis of piecewise functions, accounting for basic geometrical and mechanical properties such as the strength of the concrete applied to the tube, the connection clamping length, and the size and class of the blind-bolt's internal bolt. An assembly process is then detailed to establish the model for the elastic and inelastic behaviour of the component. Comparisons of model predictions with experimental data show that the proposed model can predict with sufficient accuracy the response of the component. The model furthers the development of a full and detailed design method for an original connection technology.

Performance Evaluation of Caching in PON-based 5G Fronthaul (PON기반 5G 프론트홀의 캐싱 성능 평가)

  • Jung, Bokrae
    • Journal of Convergence for Information Technology
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    • v.10 no.1
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    • pp.22-27
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    • 2020
  • With the deployment of 5G infrastructure, content delivery network (CDN) will be a key role to provide explosive growing services for the independent media and YouTube which contain high-speed mobile contents. Without a local cache, the mobile backhaul and fronthaul should endure huge burden of bandwidth request for users as the increase number of direct accesses from contents providers. To deal with this issue, this paper fist presents both fronthaul solutions for CDN that use dark fibers and a passive optical network (PON). On top of that, we propose the aggregated content request specialized for PON caching and evaluate and compare its performance to legacy schemes through the simulation. The proposed PON caching scheme can reduce average access time of up to 0.5 seconds, 1/n received request packets, and save 60% of backhaul bandwidth compared to the no caching scheme. This work can be a useful reference for service providers and will be extended to further improve the hit ratio of cache in the future.

An development of framework and a supporting tool for organizing Grouped Folksonomy (그룹화된 폭소노미 구축을 위한 프레임워크와 지원도구의 개발)

  • Kang, Yu-Kyung;Hwang, Suk-Hyung
    • Journal of the Korea Society of Computer and Information
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    • v.16 no.5
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    • pp.109-125
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    • 2011
  • A folksonomy is a new classification approach for organizing information by users to freely attach one or more tags to various resources published on the web. Recently, in order to provide useful services and organize the folksonomy data, many collaborative tagging systems based on folksonomy offer additional functionalities for grouping each elements of a folksonomy. In this paper, organization framework for grouped folksonomy is proposed. That is, we suggest the grouped folksonomy model that is an extended folksonomy with the concept of "group" and fundamental operations(Group Aggregation, Group Composition, Group Intersection, Group Difference) for grouping of folksonomy elements. Also, we developed a supporting tool(GFO) that constructs grouped folksonomy and executes fundamental operations. And we introduce some cases using the fundamental operations for grouping of each elements of folksonomy. Based on suggested our approach, we can construct grouped folksonomy and organize and extract useful information from the folksonomy data by grouping each elements of a folksonomy.

Leak Detection and Evaluation for Power Plant Boiler Tubes Using Acoustic Emission (음향방출을 이용한 보일러튜브 누설평가)

  • Lee, Sang-Guk
    • Journal of the Korean Society for Nondestructive Testing
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    • v.24 no.1
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    • pp.45-51
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    • 2004
  • Boiler tubes in power plants are often leaked due to various material degradations including creep and thermal fatigue damage under severe operating conditions such as high temperature and high pressure over an extended period of time. To monitor and diagnose the tubes on site and in real time, the acoustic emission (AE) technology was applied. We developed an AE leak detection system, and used it to study the variation of AE signal from the on-site tubes in response to the changes in the boiler operation condition and to detect the locations of leakage based on it. Detection of leak was performed by acquiring and evaluating the signals in separate regimes of high and low frequency signal. As a result of these studies, we found that on-line monitoring and detection of leak location for boiler tubes is possible using the developed system. Thus, the system is expected to contribute to the safe operation of power plants, and prevent economic losses due to potential leak.

A grid-line suppression technique based on the nonsubsampled contourlet transform in digital radiography

  • Namwoo Kim;Taeyoung Um;Hyun Tae Leem;Bon Tack Koo;Kyuseok Kim;Kyu Bom Kim
    • Nuclear Engineering and Technology
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    • v.55 no.2
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    • pp.655-668
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    • 2023
  • In radiography, an antiscatter grid is a well-known device for eliminating unexpected x-ray scatter. We investigate a new stationary grid artifact suppression method based on a nonsubsampled contourlet transform (NSCT) incorporated with Gaussian band-pass filtering. The proposed method has an advantage that extracts the Moiré components while minimizing the loss of image information and apply the prior information of Moiré component positions in multi-decomposition sub-band images. We implemented the proposed algorithm and performed a simulation and an experiment to demonstrate its viability. We did this experiment using an x-ray tube (M-113T, Varian, focal spot size: 0.1 mm), a flat-panel detector (ROSE-M Sensor, Aspenstate, pixel dimension: 3032 × 3800 pixels, pixel size: 0.076 mm), and carbon graphite-interspaced grids (JPI Healthcare, 18 cm × 24 cm, line density: 103 LP/inch and 150 LP/inch, ratio: 5:1, focal distance: 65 cm). Our results indicate that the proposed method successfully suppressed grid artifacts by reducing them without either reducing the spatial resolution or causing negative side effects. Consequently, we anticipate that the proposed method can improve image acquisition in a stationary grid x-ray system as well as in extended x-ray imaging.

Preliminary Analysis of the CANDU Moderator Thermal-Hydraulics using the CUPID Code (2상 유동 해석코드 CUPID를 이용한 CANDU 원자로 감속재 열수력 예비해석)

  • Park, Sang Gi;Lee, Jae Ryong;Yoon, Han Young;Kim, Hyoung Tae;Jeong, Jae Jun
    • Journal of Energy Engineering
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    • v.21 no.4
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    • pp.419-426
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    • 2012
  • A transient, three-dimensional, two-phase flow analysis code, CUPID, has been developed in KAERI. In this work, we performed a preliminary analysis using the CUPID code to investigate the thermal-hydraulic behavior of the moderator in the Calandria vessel of a CANDU reactor. At first, we validated the CUPID code using the three experiments that were performed at Stern Laboratories Inc. To avoid the complexity to generate computational mesh around the Calandria tube bundles, a porous media approach was applied for the region. The pressure drop in the porous media zone was modeled by an empirical correlation. The results of the calculations showed that the CUPID code can predict the mixed flow pattern of forced and natural convection inside the Calandria vessel very well. Thereafter, the analysis was extended to a two-phase flow condition. Also, the local maximum temperature in the Calandria vessel was plotted as a function of the injection flow rate, which may be utilized to predict the local subcooling margin.

Urinary Estrone Sulfate for Monitoring Pregnancy of Dairy Cows

  • Yang, C.J.;Wu, L.S.;Tseng, C.M.;Chao, M.J.;Chen, P.C.;Lin, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.9
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    • pp.1254-1260
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    • 2003
  • The purpose of this study firstly was conducted to establish a radioimmunoassay (RIA) of estrone sulfate ($E_1S$), secondly to monitor the reproductive status of dairy cows using their urine samples. Urine and blood samples were collected in series within a day from four pregnant Holstein-friesian cows to evaluate the relationship between $E_1S$ levels in blood and urine with or without urinary creatinine basis. The urine was then collected biweekly from three cows in estrous and those artificially inseminated; collection from pregnant cows was made on a monthly basis. Results indicated that sensitivity for the $E_1S$ RIA was 5 pg/tube and the recovery rate was 100%. The daily urinary creatinine concentrations fluctuated within a day, but changes were slighter in midday, whereas the changes of concentrations of $E_1S$ in urine were relatively smaller. The concentrations of serum $E_1S$ during the estrous cycle were undetectable due to the limitation of assay, but the urinary $E_1S$ level could be measured with no obvious changes during the cycle. The urinary $E_1S$ levels increased remarkably around 7.7 to 8.3 ng/ml, 80 to 100 days after pregnancy but the serum $E_1S$ levels did not elevate until 120 to 150 days. The level of $E_1S$ increased gradually during pregnancy and eventually reached its peak before parturition at around 40 ng/ml and finally decreased to its basal level 2 days postparturition. During pregnancy, $E_1S$ concentrations of urine increased earlier than those in blood. The correlation coefficients between urinary and serum $E_1S$ concentration during pregnancy and postparturm were higher than those adjusted with creatinine (creatinine ratio). The concentrations of $E_1S$ in urine could be maintained unchanged for 8 days storing the samples in room temperature, which was extended to 8 days when the samples were pretreated by boiling for 30 minutes or treated with autoclave. In conclusion urinary $E_1S$ concentrations can be used directly for monitoring the pregnant status and fetal viability of dairy cows and can assist accurate confirmation of pregnancy in cows at least 80 to 100 days after insemination much earlier than by serum $E_1S$.

Monitoring the Reproductive Status of Dairy Cows by Urinary Pregnanediol Glucuronide

  • Yang, C.J.;Wu, L.S.;Liu, S.H.;Lin, J.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.4
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    • pp.460-466
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    • 2004
  • This study was undertaken with the aim to establish a reliable radioimmunoassay (RIA) system for urinary pregnanediol glucuronide (PdG) and to employ it for monitoring the reproductive status of dairy cows. Urine and blood samples were collected from the Holstein cows both pregnant and non-pregnant. The samples were then investigated for evaluating the relationship between progesterone ($P_{4}$) in blood and PdG in urine adjusted with or without urinary creatinine basis. Biweekly urine collection was employed for three cows in estrous and those artificially inseminated, while urine from pregnant cows was collected on a monthly basis. P_{4}$ and PdG levels were measured by enzymeimmunoassay (EIA) and RIA techniques, respectively. Our results indicated the sensitivity of PdG for RIA being 35 pg/tube and the recovery rate of 100%. Urinary creatinine concentrations also fluctuated within a day, but change at midday was not noteworthy. Regardless of the time of urination the change in concentrations of PdG was relatively smaller and did not vary significantly. The urinary PdG concentration showed periodic changes as that with serum P_{4}$ levels during the cow's estrus cycle. The correlation coefficient rose when creatinine level in urine was adjusted but the change was also not significant. The concentrations of PdG during the luteal phase were detected between 8.2 and 17.4 ng/ml, three to five times higher than that in the follicular phase. The concentration of PdG from pregnant cows (21 days after conception) was three to four times higher than in the nonpregnant cows. Our finding suggests that the determination of urinary PdG could be reliably employed for early pregnancy detection. The urinary PdG level continued to raise until 30 days pre-partum while the concentration reached its peak at 30 ng/ml, after which it started to fall 18 to 30 days before parturition and finally fell to its nadir value one week after parturition. As the correlation coefficient between the urinary PdG and serum P_{4}$ was higher than that corrected by urinary creatinine it can be suggested that the adjustment is not needed. The concentrations of urinary PdG could be maintained stably for 2 days in urine samples stored at room temperature and extended to 8 days when the samples were pretreated by boiling for 30 minutes. In conclusion urinary PdG concentration even without the need for creatinine basis adjustment can be used directly for monitoring the reproductive status of dairy cows.

Inflammatory Polyps in 2 Cats (두 마리 고양이에서의 염증성 용종 예)

  • Ko, Seung-Bo;Jung, Ji-Youl;Kim, Jae-Hoon;Kang, Sang-Chul;Yoon, Jeong-Sik;Yang, Jung-Hwan;Shin, Yong-Eun;Kim, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.27 no.5
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    • pp.622-625
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    • 2010
  • Inflammatory polyps in feline ear are nonneoplastic, inflammatory growths that arise from the middle ear or the eustachian tube and extended into the pharynx or external ear canal. Two 2-year-old female Russian blue cats showed 2-3 weeks history of aural discharge, crust formation in external ear, and head or ear shaking. Two masses were surgically excised from ear canal, and submitted for diagnosis. Histopathologically, these masses were covered with hyperplastic ciliated epithelium or nonkeratinizing squamous epithelium with partial erosion and ulceration. The core of masses was consisted of proliferated connective tissue and massive infiltration of mononuclear cells. Immunohistochemically, about 90% of infiltrated mononuclear cells demonstrated CD3 positive T cell. According to both polymerase chain reaction (PCR) and reverse transcriptase-PCR, tissues samples were negative for feline viral pathogens. Based on the clinical, gross, histopathologic findings, these two cases were diagnosed as inflammatory polyps originated from the middle ear in cats.