• Title/Summary/Keyword: Expression analysis

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Effects of Working Memory Load on Negative Facial Emotion Processing: an ERP study (작업기억 부담이 부적 얼굴정서 처리에 미치는 영향: ERP 연구)

  • Park, Taejin;Kim, Junghee
    • Korean Journal of Cognitive Science
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    • v.29 no.1
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    • pp.39-59
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    • 2018
  • To elucidate the effect of working memory (WM) load on negative facial emotion processing, we examined ERP components (P1 and N170) elicited by fearful and neutral expressions each of which was presented during 0-back (low-WM load) or 2-back (high-WM load) tasks. During N-back tasks, visual objects were presented one by one as targets and each of facial expressions was presented as a passively observed stimulus during intervals between targets. Behavioral results showed more accurate and fast responses at low-WM load condition compared to high-WM load condition. Analysis of mean amplitudes of P1 on the occipital region showed significant WM load effect (high-WM load > low-WM load) but showed nonsignificant facial emotion effect. Analysis of mean amplitudes of N170 on the posterior occipito-temporal region showed significant overall facial emotion effect (fearful > neutral), but, in detail, significant facial emotion effect was observed only at low-WM load condition on the left hemisphere, but was observed at high-WM load condition as well as low-WM load condition on the right hemisphere. To summarize, facial emotion effect observed by N170 amplitudes was modulated by WM load only on the left hemisphere. These results show that early emotional processing of negative facial expression could be eliminated or reduced by high load of WM on the left hemisphere, but could not be eliminated by high load on the right hemisphere, and suggest right hemispheric lateralization of negative facial emotion processing.

Change of Refractive Index of Air in X-band due to Atmospheric Humidity, Temperature and Pressure measured by GB-SAR Interferometry (GB-SAR 간섭기법으로 측정된 X-밴드 대기 굴절률의 상대습도, 기온 및 기압에 따른 변화)

  • Lee, Jae-Hee;Lee, Hoon-Yol;Cho, Seong-Jun;Sung, Nak-Hoon;Kim, Kwang-Eun
    • Korean Journal of Remote Sensing
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    • v.27 no.2
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    • pp.163-170
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    • 2011
  • In this paper, we analyzed the phase change of 5-triangular trihedral comer reflectors by using X-band Ground-Based Synthetic Aperture Radar (GB-SAR) system. Each reflector was set as a stationary target at a different distance from the system. We obtained total 123 full-polarization images during 40 hours continuously at 20 minute interval. Results of SAR interferometric analysis showed phase changes of maximum 2 radians and followed similar pattern with atmospheric data. Through a GB-SAR phase formula that includes refractive index in the air, we performed regression analysis for refractive index as a function of atmospheric humidity, temperature and pressure. As a result, refractive index of air in X-band showed relatively high coefficient of determination with humidity and temperature (0.72 and 0.76 on average, respectively) but not so with pressure (0.34). The refractive index of air in X -band changed by 3.14\;{\times}\;10^{-5}$ during the measuring time with a humidity range of 50% ~ 90% and a temperature range of $-1^{\circ}C$ ~ $9^{\circ}C$. We expect that a total expression of refractive index of air including humidity, temperature and pressure can be calculated when more extensive data would be collected at various atmospheric conditions.

The Development of STEAM Program with the Unit 'Energy and Transportation Technology' on the Subject of Technology.Home Economics (기술.가정 교과 '에너지와 수송 기술' 단원에서 활용할 STEAM 프로그램 개발)

  • Kim, Ki Yeol;Ham, Hyung In;Kim, Ki Soo
    • 대한공업교육학회지
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    • v.38 no.1
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    • pp.29-48
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    • 2013
  • The purpose of this research is to provide a desirable case of STEAM education utilizing 'Energy and Transportation Technology' unit on the subject of technology home economics. The middle school students learned with the integrated approach based on STEAM integrated education, and through production process they had the opportunity for application and expression in diverse forms. The results of this process to achieve the purpose are as follows. First, as the procedural model to develop STEAM program utilizing 'Energy and Transportation Technology' unit on the subject of technology home economics, the five stages: preparation, development, verification, practice, and evaluation, were presented. The preparation stage was composed of requirement analysis, the selection of program subject, and STEAM program curriculum analysis, the selection and organization of STEAM program lesson. The development stage was composed of the development of lesson plan, multimedia teaching materials, worksheet and worksheet answer key. In the verification stage, the verification of validity by experts was conducted, and in the practice stage, the developed program was applied to the middle school students in the educational field, and in the evaluation stage, based on the evaluations received from learners and teachers, it was revised and supplemented. Second, the STEAM program was developed into the program summary map, lesson plan, multimedia teaching materials, worksheet and worksheet answer, etc., and after the validity was secured through experts' verification, it was revised and supplemented and applied to actual classes. Third, the results of the learners' evaluation of the developed STEAM program showed that the degree of satisfaction with the program was high with the average score of the entire questions being 4.00 on a five-point scale. As the teachers also evaluated the developed STEAM program as very effective, the opinions of learners and teachers were collected and the program was finally improved and completed.

Proteomic Analysis of Cytokinin Induced Proteins in Arabidopsis (단백체를 이용한 애기장대 Cytokinin 유도 단백질의 분석)

  • Liang Ying-Shi;Cha Joon-Yung;Ermawati Netty;Jung Min-Hee;Bae Dong-Won;Lee Chang-Won;Son Dae-Young
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.251-256
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    • 2005
  • Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching, We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.

Analysis of Seed Hair Formation Related Genes by EST Profiling in Carrot (Daucus carota var. sativa) (EST profiling을 통한 당근(Daucus carota var. sativa)의 종모 형성에 관련된 유전자 분석)

  • Hwang, Eun-Mi;Oh, Gyu-Dong;Shim, Eun-Jo;Jeon, Sang-Jin;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.1039-1050
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    • 2010
  • Carrot is one of the useful crops used abundantly in cooking in Western as well as Asia regions such as China and Korea. However, seed coats have hairs which should be removed to increase germination rate. Furthermore, because of seed hairs, farmers face several additional losses, such as time consumption, manpower, capital and so on, for seed handling. To prevent these problems, study of gene related hair formation using short-hair seed lines is required. We analyzed genes related to hair formation from seed through expressed sequenced tag (EST) profiling, based on the fact that the development of carrot seed hair is related to cellulose synthesis pathway in secondary cell wall synthesis stage. To study the gene expression related to hair formation of the carrot seed, a cDNA library was constructed by using the early maturation stage of the short-hair line (659-1) and hairy seed line (677-14). In short-hair (659-1) and hairy seed (677-14) lines, results from of EST profiling through BLASTX search analysis using the NCBI database showed that 172 and 224 unigenes had significant homology with known protein sequences, whereas 233 and 192 unigenes were not, respectively. All ESTs were grouped into 16 categories according to their putative functions. Twenty nine unigenes among all ESTs were considered to be genes regulating seed hair development from cellulose synthesis pathway during secondary cell wall synthesis stage; in results, 14 unigenes related to seed hair development were found only in hairy seed line.

Comparative Analyses for Aroma and Agronomic Traits of Native Rice Cultivars from Central Asia

  • Sarhadi, Wakil Ahmad;Hien, Nguyen Loc;Zanjani, Mehran;Yosofzai, Wahida;Yoshihashi, Tadashi;Hirata, Yutaka
    • Journal of Crop Science and Biotechnology
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    • v.11 no.1
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    • pp.17-22
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    • 2008
  • Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

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Developmental Changes of Adolescent's Big Five Personality Factors (Big 5 성격요인에 따른 청소년 성격특성의 발달적 변화)

  • Jang, Eun-Ji;Choi, Eun-Sil
    • The Journal of the Korea Contents Association
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    • v.17 no.10
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    • pp.307-321
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    • 2017
  • This study examined the developmental changes of adolescent personality regarding personality traits of Big 5 model in 2,260 middle and high school students. We found that there was a difference in the developmental change of personality 5 factor according to sex and grade. In addition, we investigated the timing and characteristics of adolescent problem behavior by analyzing the sub - factors of neurosis. Analysis was used for One-Way ANOVA. if there is significant differences we proceeded post hoc tests. The results of this study are as follows; first, The five personality traits of the adolescents showed differences according to gender. The girls were higher than boys in the Openness, Conscientiousness and Extraversion. In contrast, the boys were higher than girls in the Neuroticism. Second, There was a difference in the characteristics of five personality traits of adolescents according to grade. Third, Gender-specific developmental grade tendency characteristics of adolescent personality trait shows a different pattern in gender. Especially, in the analysis by gender and grade boys showed that personality traits prominent in the middle school Grade 2. likewise, girls showed that personality traits prominent in the high school Grade 3. Then, associated with Neuroticism the Externalizing behavior problems was found to be expressed in the middle school Grade 1 and 2. likewise, the Internalizing behavior problems was found to be expressed in high school Grade 3. Therefore, this study was able to determine the current developmental change in personality traits adolescence of our country. Also it found that mental health problems can be a different expression depending on gender and grade.

Analysis of Bacterial Spot Disease in Red Pepper Caused by Increase of CO2 Concentration (CO2 농도 상승 효과에 의한 고추 세균점무늬병 발병 양상 분석)

  • Jang, Jong-Ok;Kim, Byung-Hyuk;Moon, Doo-Gyung;Koh, Sang-wook;Joa, Jae-Ho
    • Microbiology and Biotechnology Letters
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    • v.46 no.1
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    • pp.77-84
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    • 2018
  • An increase in $CO_2$ will affect plant pathogenic microorganisms, the resistance of host plants, and host-pathogen interactions. This study used Capsicum annuum and Xanthomonas euvesicatoria, a pathogenic bacterium of pepper, to investigate the interactions between hosts and pathogens in conditions of increased $CO_2$ concentrations. Our analysis of disease resistance genes under 800 ppm $CO_2$ using quantitative RT-PCR showed that the expression of CaLRR1, CaPIK1, and PR10 decreased, but that of negative regulator WRKY1 increased. Additionally, the disease progress and severity was higher at 800 ppm than 400 ppm $CO_2$. These results will aid in understanding the interaction between red pepper and X. euvesicatoria under increased $CO_2$ concentrations in the future.

Codon usage analysis of rice glutelin genes (쌀 저장 단백질 글루텔린 유전자 암호 분석)

  • Shin, Yun-Cheol;Kim, Ju-Kon;Nahm, Baek-Hie
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.517-524
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    • 1993
  • To characterize glutelins, the most abundant storage protein in rice, 13 complete coding sequences of glutelin genes from the database were analyzed. According to the phylogenic analysis, these genes could be classified into 5 groups, Group I to V. The degrees of homology were calculated to be in the range of 90 to 60%, but the patterns of hydrophobicity were similar in all the groups. Also, each group was found to have similar amino acid composition with variations in lysine content from 2.5 to 3.6% due to the point mutation of arginine to lysine. The isoelectric points of mature proteins and their basic chains of all the groups showed the value of about 9.0 and 10.0, respectively, while the isoelectric points of acidic chains in these groups showed the distinct value of 6.6, 6.7, 7.2, 8.4 and 7.9. The plot of the fraction of G+C at synonimous site in codons (GC3s) against effective codon numbers suggest no major difference in translational efficiency in the expression of glutelin multigenes.

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Identification of Cuts-specific Myogenic Marker Genes in Hanwoo by DNA Microarray (DNA Microarray 분석을 통한 한우 부위별 특이 마커 유전자의 발굴)

  • Lee, Eun-Ju;Shin, Yu-Mi;Lee, Hyun-Jeong;Yoon, Du-Hak;Chun, Tae-Hoon;Lee, Yong-Seok;Choi, In-Ho
    • Journal of Animal Science and Technology
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    • v.52 no.4
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    • pp.329-336
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    • 2010
  • Myogenic satellite cells (MSCs) are mononuclear, multipotent progenitors of adult skeletal muscle possessing a capacity of forming adipocyte-like cells (ALC). To identify the skeletal muscle type-specific myogenic and adipogenic genes during MSCs differentiation, total RNA was extracted from bovine MSCs, myotube-formed cell (MFC), and ALC from each of Beef shank, Longissimus dorsi, Deep pectoral, and Semitendinosus. DNA microarray analysis (24,000 oligo chip) comparing MSCs with MFC and ALC, respectively, revealed 135 differentially expressed genes (> 4 fold) among four cuts. Real-time PCR confirmed expression of 29 genes. Furthermore, the whole tissue sample RNAs analysis showed 6 differentially expressed genes in Beef shank. Among which, 1 gene in MSCs, 4 in MFC, and 1 in ALCs were highly expressed. This study will provide an insight for better understanding the molecular mechanism of differentiation of skeletal muscle type-specific MSCs. The identified genes may be used as marker to distinguish skeletal muscle types.