• 한국작물학회지
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• 제60권1호
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• pp.33-40
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• 2015

재배환경에 따른 단백질 발현을 이차원전기영동을 이용하여 단백질 발현양상을 확인하고 이미지 분석을 통하여 단백질 발현 정도를 수치화하여 비교 분석한 결과, 재배환경에 따른 단백질 발현 양상은 전반적으로 매우 유사하였으며, 주요 단백질의 발현에는 차이가 없었다. 하지만 논과 밭, 즉 재배환경은 단백질의 발현 정도에는 영향을 미치는 것을 확인 할 수 있었다. 공시품종들 중에서 백천콩은 다른 세 품종과는 다르게 논에서 단백질 발현 정도가 밭에서의 단백질 발현 정도보다 높았다. 이 결과로 보아 품종마다 재배환경에 따른 단백질의 발현 정도에 차이가 있는 것으로 사료된다. 향후, 단백질 총 함량을 재배환경에 따라 비교하고 또한 질량분석을 통하여 증가 또는 감소되는 단백질의 기능을 확일 할 필요가 있다고 사료된다.

• Journal of Microbiology
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• 제44권2호
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• pp.217-225
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• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.

• 복식문화연구
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• 제14권1호
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• pp.128-141
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• 2006

The purposes of this study are to consider characteristics of 'movement expression' that are not discovered in preceeding design studies in order to suggest 'movement expression' as a design formative principal and to make clothes which follows this idea. For the method of this study I used various literature about 'movement expression' as theoretical background and analyzed modern clothing in which 'movement expression' is expressed by collecting magazines including foreign collections from 1989 to 2004 and searching the internet. The results obtained from this study is as follows. First, clothing in which 'movement expression' is expressed removes monotony and tedium of clothing design, provides visual interest and satisfaction for clothing, and delivers designers' concept effectively by attracting a gaze quickly. And It is suggested a new division of 'movement expression' through analysis of the 'movement expression' expressed in clothing. It included the intentional movement by wearers, which is the 'movement expression' of broader meaning such as changes of form or position by a certain operation and changes of existence or function. This movement is not fixed and repeated but changes continuously. As a result of including the design expressing the intentional movement by wearers, one's own personality can be expressed more effectively. It is presented a design which has changeable movement expression continuously.

• 한국의류학회지
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• 제29권11호
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• pp.1410-1420
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• 2005

The purpose of this study was to analyze characteristics of effective expression on T-shirts pattern by peirce's semiotic theory. The methods of this study was to analyze 721 T-shirts patterns in designer's collection from 2000 to 2004. The results of this study were as follows: First of all, iconic expression showed high frequency after 2000. Characteristics of effective expression were as follows: In geometric expression, repetition was presented as a symbolic sign by regular proportion and rule of dot, line, plane, irregularity, mixture of irregular dot, line, plane. Mixed expression was presented as a iconic and symbolic sign by collage, mixture of dot, line, plane, icon and letter. In iconic expression, simplification of iconic sigrl was presented as a iconic sign by simplification of form, color, texture, realistic expression using digital as a iconic sign, and symbolic face, body as a symbolic sign. Pop art's expression was presented as a iconic sign by a cartoon and commercial character and illusion was presented as a iconic sign. In letter's expression, brand logo was presented as a symbolic sign by transformation of letter's design, a symbolic sign of numeral by transformation of size, thickness, form, color. Symbolic message phrase was presented by slogan, fashion trend, brand image, descriptive indication message as a index sign by using icon or singleness. In conclusion, characteristics of effective expression on T-shirts pattern will present not only the theoretical foundation to raise the value added, but also the information about beauty sense of times, political and social value.

• Asian Pacific Journal of Cancer Prevention
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• 제15권16호
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• pp.6761-6766
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• 2014

Background: CD44v6 (CD44 variant exon 6) is the chief CD44 variant isoform regulating tumor invasion, progression, and metastasis. The prognostic value of CD44v6 expression in non small cell lung cancer (NSCLC) has been evaluated in many studies, but the results have remained controversial. Thus, we performed a meta-analysis of currently available studies to investigate the prognostic value of CD44v6 expression in NSCLC patients and the relationship between the expression of CD44v6 and clinicopathological features. Materials and Methods: Two independent reviewers searched the relevant literature in Pubmed, Medline and Embase from 1946 to January 2014. Overall survival (OS) and various clinicopathological features were collected from included studies. This meta-analysis was accomplished using STATA 12.0 and Revman 5.2 software. Pooled hazard ratios (HRs) with 95% confidence intervals (95%CIs) were calculated to estimate the effects. Results: A total of 921 NSCLC patients from ten studies met the inclusion criteria. The results showed that CD44v6 high expression was a prognostic factor for poor survival (HR=1.91, 95%CI=1.12-3.26, p<0.05). With respect to clinicopathological features, CD44v6 high expression was related to histopathologic type (squamous cell carcinoma versus adenocarcinoma: OR=2.72, 95%CI=1.38-5.38, p=0.004), and lymph node metastasis (OR=3.02, 95%CI=1.93-4.72, p<0.00001). Conclusions: Our results suggested CD44v6 high expression as a poor prognostic factor for NSCLC, and CD44v6 expression is associated with lymph node metastasis and histopathologic type. Therefore, CD44v6 expression can be used as a novel prognostic marker in NSCLC cases.

• Asian-Australasian Journal of Animal Sciences
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• 제30권3호
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• pp.328-337
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• 2017

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.

• 전자공학회논문지CI
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• 제42권1호
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• pp.1-7
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• 2005

마이크로어레이 데이터의 클러스터 분석은 생물학적으로 연관성 있는 유전자 그룹을 찾기 위해 종종 사용되는 방법이다. 기능적으로 연관된 유전자들이 대개 유사한 발현 패턴을 나타내는 특징을 이용하여 유사한 발현 프로파일을 가진 유전자 그룹을 찾아냄으로써 알려지지 않은 유전자들의 기능을 같은 그룹에 속한 다른 유전자로부터 유추할 수 있기 때문이다. 본 논문에서는 클러스터 분석을 위해 시드 클러스터링 알고리즘을 새로이 제안하고, 이 방법을 마이크로어레이 데이터 분석에 적용해본다. 시드 클러스터링 방법은 주어진 데이터를 계산적으로 분석하여 시드 패턴을 자동 추출하고, 이러한 시드 패턴을 목적 클러스터의 프로토타입 벡터로서 간주하여 클러스터를 생성하는 방법이다. 이러한 시드 클러스터링 방법은 수학적 원리에 기초하고 있기 때문에, 매우 체계적인 방법으로 안정적이며 일관성 있는 클러스터링 결과를 생성할 수 있다. 또한, 실제 마이크로어레이 데이터 분석에 적용해본 결과 데이터에 내재된 각 클러스터를 대표하는 시드 패턴을 매우 효과적으로 자동 추출할 수 있었으며, 클러스터링 결과 또한 타 방법에 비해 다소 우월한 경향을 나타내었다.

• 한국한의학연구원논문집
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• 제8권2호통권9호
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• pp.95-107
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• 2002

Yukmijihwang-tang(YM) is a noted herbal prescription in Chinese and Korean traditional medicines, and it has been known to reinforce the vital essence and has been widely used for a variety of disease such as stroke, osteoporosis, anti-tumor, and hypothyrodism. Regarding its traditional use, YM has been known to reinforce the Yin (vital essence) of liver and kidney. Also it has been known to reinforce nutrition and biological function in brain. Recently, studies suggested that YM increase antioxidant activities and exert the protective effect against oxidant-induced liver cell injury. We investigated the high-throughput gene expression analysis on the Yukmijihwang-tang administrated in SD rats. Microarray data were validated on a limited number of genes by semiquantitative RT-PCR and Western blot analyses. The recent availability of microarrays provides an attractive strategy for elaborating an unbiased molecular profile of large number of genes in drug discovery This experimental approach offers the potential to identify molecules or cellular pathways not previously associated with herbal medicine. Total RNA from normal control brain and Yukmijihwang-tang administrated brain were hybridized to microarrays containing 10,000 rat genes. The 52 genes were found to be up-regulated(twice or more) excluding EST gene. The nine genes were found to be down-regulated(twice or more) excluding EST gene. Gene array technology was used to identify for the first time many genes expression pathway analysis that arecell cycle pathway, apoptosis pathway, electron transport chain pathway, cytoplasmic ribosomal protein pathway, fatty acid degradation pathway, and TGF-beta signaling pathway. These differentially expressed genes pathway analysis have not previously been iavestigated in the context of herbal medicine efficacy and represent novel factors for further study of the mechanism of herbal medicine efficacy.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.173-173
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• 2003

With the introduction of DNA microarrays, a high throughput analysis of gene expression is now possible as a replacement to the traditional time-consuming Southern-blot analysis. This cDNA microarray should be ahighly favored technology in the area of molecular toxicology or analysis of environmental stresses.In this study, therefore, we developed a novel cDNA microarray for analyzing stress-specific responses in japanese Medaka fish. In the design and fabrication of this stress specific functional cDNA microarray, 123 different genes in Medaka fish were selected from eighteen different stress responsive groups and spotted on a 25${\times}$75 mm glass surface. After exposure of the fish to bisphenol A which is the one of the well-known endocrine disrupting chemicals (EDCs), over 1 or 10 days, the responses of the DNA chip were found to show distinct expression patterns according to the mode of toxic actions from environmental toxicants. As a results, they showed specific gene expression pattern to bisphenol A, additionally, the chemical spesific biomarkers could be suggested based on the chip analysis data. Therefore, this chip can be used to monitor stress responses of unknown and/or known toxic chemicals using Medaka fish and may be used for the further development of biomarkers by utilizing the gene expression patterns for known contaminants.

• 대한한의학회지
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• 제20권1호
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• pp.91-105
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• 1999

The effects of Yinjin and Yinjinsaryongsangagambang on a DNA damaging agent, etoposide-induced apoptosis, cell viability, cell cycle progression, and mRNA expression of apoptosis-related genes of human hepatocyte cell line HepG2 were investigated using tryphan blue exclusion assay, MTT assay, flow cytometry, immunocytometric analysis of PCNA, and quantitative RT-PCR analysis. MTT assay showed that Yinjin and Yinjinsaryongsangagambang increases cellular viability of HepG2 cells in a dosage-dependent manner. Stimulation of cell cycle progression by Yinjin or Yinjinsaryongsangagambang was detected by flow cytometric analysis of the DNA content and immunocytometric analysis of PCNA expression. A significant reduction of a DNA-damaging agent, etoposide-induced apoptosis were found in both Yinjin and Yinjinsaryongsangagambang-treated cells in dosage-dependent manner. In overall, 3-fold reduction of apoptosis was recognized in $10.0\;{\mu}g/ml$ of Yinjin or Yinjinsaryongsangagambang-treated cells compared to untreated cells. Although the difference is not significant, Yinjinsaryongsangagambang showed slightly higher effect on the inhibition of apoptosis than Yinjin. From flow cytometric analysis of apoptosis, while 39.9% of untreated cells showed etoposide-induced apoptotic cell death, only 19.6% or 17.4% of Yinjin or Yinjinsaryongsangagambang-treated cells were fond at apoptotic sub G1 phase, respectively. Interestingly, strong induction of Gadd45-mRNA was observed from Yinjin or Yinjinsaryongsangagambang-treated cells. However, no changes in expression levels of p53 and Waf1 were detected, demonstrating that induction of Gadd45 mRNA expression by Yinjin or Yinjinsaryongsangagambang occurs by p53-independent mechanism. Marked mRNA inductions of two apoptosis-inhibiting genes, Bcl-2 and Bcl- XL, were found in both Yinjin or Yinjinsaryongsangagambang-treated HepG2 cells while no changes was detected in expression levels of an apoptosis-promoting gene, Bax.