• Title/Summary/Keyword: Expression Strategies

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Basic Concept of Gene Microarray (Gene Microarray의 기본개념)

  • Hwang, Seung Yong
    • Korean Journal of Biological Psychiatry
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    • v.8 no.2
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    • pp.203-207
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    • 2001
  • The genome sequencing project has generated and will continue to generate enormous amounts of sequence data including 5 eukaryotic and about 60 prokaryotic genomes. Given this ever-increasing amounts of sequence information, new strategies are necessary to efficiently pursue the next phase of the genome project-the elucidation of gene expression patterns and gene product function on a whole genome scale. In order to assign functional information to the genome sequence, DNA chip(or gene microarray) technology was developed to efficiently identify the differential expression pattern of independent biological samples. DNA chip provides a new tool for genome expression analysis that may revolutionize many aspects of biotechnology including new drug discovery and disease diagnostics.

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High-throughput identification of chrysanthemum gene function and expression: An overview and an effective proposition

  • Nguyen, Toan Khac;Lim, Jin Hee
    • Journal of Plant Biotechnology
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    • v.48 no.3
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    • pp.139-147
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    • 2021
  • Since whole-genome duplication (WGD) of diploid Chrysanthemum nankingense and de novo assembly whole-genome of C. seticuspe have been obtained, they have afforded to perceive the diversity evolution and gene discovery in the improved investigation of chrysanthemum breeding. The robust tools of high-throughput identification and analysis of gene function and expression produce their vast importance in chrysanthemum genomics. However, the gigantic genome size and heterozygosity are also mentioned as the major obstacles preventing the chrysanthemum breeding practices and functional genomics analysis. Nonetheless, some of technological contemporaries provide scientific efficient and promising solutions to diminish the drawbacks and investigate the high proficient methods for generous phenotyping data obtaining and system progress in future perspectives. This review provides valuable strategies for a broad overview about the high-throughput identification, and molecular analysis of gene function and expression in chrysanthemum. We also contribute the efficient proposition about specific protocols for considering chrysanthemum genes. In further perspective, the proper high-throughput identification will continue to advance rapidly and advertise the next generation in chrysanthemum breeding.

Stress and Coping Strategies of Breast Cancer Patients and their Spouses (유방암 환자와 배우자의 스트레스와 대처방식)

  • Cha, Kyeong-Sook;Yoo, Yang-Sook;Cho, Ok-Hee
    • Asian Oncology Nursing
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    • v.12 no.1
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    • pp.20-26
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    • 2012
  • Purpose: The purpose of this study was to identify the stress and the coping strategies in breast cancer patients and their spouses. Methods: The stress level was measured by the Stress Questionnaire of Andersson & Albertsson (2000). The coping strategies were measured by the modified Lazarus & Folkman's Ways of Coping Questionnaire. The data were collected by a survey sampling 49 couples from one hospital in Seoul. The data were analyzed by t-test, ANOVA and paired t-test. Results: There was no significant differences between the stress level of breast cancer patients and their spouses. The problem-focused coping of breast cancer patients was significant higher than their spouses. The cancer patients and their spouses used problem-focused coping mode more than emotion-focused coping mode. In the problem-focused coping mode, breast cancer patients used two coping strategies - 'seeking information' and 'cognitive reconstruction' - significantly more than their spouses. In emotion-focused coping mode, the breast cancer patients used one coping strategy, 'emotional expression', significantly more than the their spouses. Conclusion: Further study needs to attempt to develop nursing interventions that could improve positive coping strategies.

Communication Strategies of Online-Based Leadership and Members' Work Engagement and Job Burnout

  • Hahm, Sang-Woo
    • Journal of Internet Computing and Services
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    • v.18 no.5
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    • pp.103-112
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    • 2017
  • Work engagement and job burnout are crucial problems in Korea, particularly in the business environment, and they also indicate people's motivation. Online-based teams in e-business also have these issues. Especially the role of the leader in this new type of teams is more crucial for members' motivation, behavior, and performance. Contingency leadership theories posit that different situations need different traits or behaviors of a leader. For an online-based team, which is internet-based team in e-business, a leader should use specific communication strategies to enhance a team's performance and members' motivation. In an online-based team and an offline-based team, the ways in which leaders motivate members will be different. This paper suggests particular communicative strategies for leaders of online situations. Online-based team members communicate using mobile phones or IT tools, and leaders mayinfluences members in virtual meetings. Moreover, online-based leadership needs to be more entrepreneurial and risk encouraging, and less conservative than offline-based leadership. In addition, online-based leadership should be energetic, decisive, adaptable, and inspiring, and furtherutilize communication tools such as being more gregarious/talkative, assertiveness, linguistic diversity and affect. This paper explains the roles of communicative elements such as coordination, expression, direction-giving/uncertainty reduction, empathy, meaning-making, satisfaction, and competence to decrease job burnout and to increase work engagement. The purpose of this study is to suggest communication strategies' differentiated influences on work engagement and job burnout in an online-based team.

Feeding strategies alter gene expression of the calpain system and meat quality in the longissimus muscle of Braford steers

  • Coria, Maria Sumampa;Reineri, Pablo Sebastian;Pighin, Dario;Barrionuevo, Maria Guadalupe;Carranza, Pedro Gabriel;Grigioni, Gabriela;Palma, Gustavo Adolfo
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.5
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    • pp.753-762
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    • 2020
  • Objective: The aim of the present study was to determine the effect of supplementing pasture-finished steers with corn silage on the expression level of the calpain system proteins and beef tenderization. Methods: Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day (Suppl) whereas the remaining 15 steers only received pasture (Contr). Carcass and meat traits were evaluated and compared between groups. Gene expression and activities of proteases (calpain 1 and calpain 2) and inhibitor (calpastatin) were measured using real-time polymerase chain reaction and casein zymography. Results: Carcass and meat traits were significantly different between feeding systems. Supplemented steers showed higher hot carcass weight (p<0.01), fat content (p = 0.02), and Warner-Bratzler shear force (p = 0.03). Furthermore, the control group showed higher protease:inhibitor ratios, at mRNA (p = 0.01) and protein levels (p<0.10). Warner-Bratzler shear force and mRNA calpains:calpastatin ratio were associated in both feeding systems (p<0.01). Conclusion: Based on the results obtained in the study, beef tenderness differences among finishing strategies could be modulated through differential expression of the calpain system proteins.

The Influence of Children's Emotional Expression and Sociability, and Their Mothers' Communication Pattern on Their Prosocial Behavior (아동의 정서 표현성과 사교성, 어머니의 의사소통 유형이 아동의 친사회적 행동에 미치는 영향)

  • Song, Ha-Na;Choi, Kyoung-Sook
    • Journal of the Korean Home Economics Association
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    • v.47 no.6
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    • pp.1-10
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    • 2009
  • This study investigated the influence of children's emotional expression and sociability, and their mothers' communication pattern on their prosocial behavior. The participants were 65 preschool children aged between 5 and 6, and their mothers. Each child-mother dyad was observed for 30 minutes in a lab setting, which was designed to evaluate the child's socioemotional competence and the mother's socialization behavior. Videotaped data were analyzed by two coders for aspects of sharing behavior, the expression of happiness, sadness, anger, anxiety, and sociability for children, and mothers' communication strategies. Results showed that children's anger and anxiety expression were the most significant predictors for their prosocial behavior. Mothers' punitive communication pattern negatively affected children's prosocial behavior. However, when compared to the children's emotional expression, its' accountability were not significant. The influence of negative emotions, and its' adverse role in interpersonal interactions are discussed.

Effects of Allicin on Cytokine Production Genes of Human Peripheral Blood Mononuclear Cells (마늘의 Allicin이 사람 단핵세포의 사이토카인 생산 유전자의 발현에 미치는 영향)

  • 박란숙
    • The Korean Journal of Food And Nutrition
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    • v.15 no.3
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    • pp.191-196
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    • 2002
  • The effect of allicin, the major component of garlic (Allium sativum), on the gene expression profiles of peripheral blood mononuclear cells from healthy donors was analyzed. DNA microarray which can detect expression signal of 862 genes revealed that allicin induced the expression of cytokine, chemokine, and immune-related genes in peripheral blood mononuclear cells. In contrast, allicin repressed the expression of adaptive immune-related genes, which are expressed in T helper 1 Iymphocytes. Simultaneous inhibitory and stimulatory effects of allicin were found on inflammatory cells. It is likely that allicin down-regulated the expression of specific genes that were previously up-regulated in resting cells, suggesting a new mechanism by which they exert positive and negative effect. Considering the broad and renewed interest in allicin, the profiles we describe here will be useful in designing more specific and efficient treatment strategies.

Anger Expression and Health Behavior in Patients with Coronary Arteries Disease (관상동맥질환자의 분노표현유형과 건강행위)

  • Hong, Eun-Mi;Park, Jin-Hee
    • Journal of Home Health Care Nursing
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    • v.21 no.2
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    • pp.101-109
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    • 2014
  • Purpose: The purpose of this study was to identify the difference in health behavior according to the anger expression styles in patients with coronary arteries disease. Methods: Participants included 99 patients with coronary artery disease who were treated with a percutaneous coronary intervention in K University Hospital in Seoul, from January to March 2012. The survey data were collected using the Anger Expression Inventory Korea Version and the Health Promoting Lifestyle Profile Version 2. The data were analyzed using descriptive statistics, acluster analysis, chi-square test,and ANOVA with the PASW 19.0program. Results: The anger expression styles identified from the cluster analysis were anger-control type(43.3%), anger-in/out type(42.4%), and high anger expression type(14.4%). The total score of the Health Promoting Life style Profile for the anger-control type was significantly higher than the other two types. Additionally, anger-control type showed significantly higher scores than the other two types in all domains of the Health Promoting Life style Profile. Conclusion: These results indicated that higher levels of anger-in and anger-out increased the risk of adverse health behavior and that anger control strategies could have some benefit in reinforcing healthy behavior in patients with coronary artery disease.

An Analysis of Contents on Written Clothing Described in Advertisement of Women's Underwear (여성 속옷 광고에 나타난 문자의상의 내용 분석)

  • 김미영;한명숙
    • The Research Journal of the Costume Culture
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    • v.5 no.2
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    • pp.181-193
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    • 1997
  • The purpose of this study is to review, from the point of view that the advertisements are cultural system which transfers the life styles or value systems of a certain time, the expression styles by analyzing written clothing described in underwear advertisements of women's magazines targeting the women aged from twenties to forties, that advertisements are trying to represent. Accordingly, this study is intended to provide the underwear industry with useful information for implementing sales strategies by verifying the determining factors in selecting and wearing of underwear. The result of this study is as follows : First, the headlines of advertisements could be analyzed through merchandise, situation and consumers and were categorized by the fourteen different expression styles such as functionality, sense of beauty, tradition, future, family, exotic, globalization, environment, sensuality, youth, individuality, health, newness and top-class. Therefore, the advertisers choose and provide the various expression styles in order to maximize the delivering power of the underwear advertisements. Second, frequently used expression styles have been analyzed through frequency analysis of expression styles and, as a result, the important factors of choosing and wearing underwear have been verified. In addition, the fact that the ways of thinking of modern society and value systems are implied in the expression of underwear advertisements has been found.

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Effects of Children's Temperament, Emotional Intelligence, Maternal Socialization Beliefs and Strategies on Preschoolers' Pro-social Behavior (유아의 친사회적 행동발달에 관한 연구 - 유아의 사회인구학적 특성 및 기질, 정서지능과 어머니의 양육신념 및 사회화 전략을 중심으로 -)

  • Hong, Hae-Ran;Ha, Ji-Young;Seo, So-Jung
    • Korean Journal of Child Studies
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    • v.29 no.6
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    • pp.15-33
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    • 2008
  • The purpose of this study was to investigate which variables predicted preschoolers' pro-social behavior. Subjects consisted of 250 preschooler-mother pairs; children were 3 to 6 years of age Children's emotional intelligence and pro-social behavior were assessed by classroom teachers. Data on the mothers' socialization beliefs and strategies as well as children's temperament were gathered through maternal self-reported questionnaires. Data were analyzed by descriptive statistics, t-tests, correlation and regression analyses. Results showed that children's temperament (withdrawal predisposition) and emotional intelligence (self emotional expression) predicted children's pro-social behavior. Mothers' perceptions of the importance of pro-social behavior correlated negatively with children's pro-social behavior.

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