• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.829-832
• /
• 2001

Phage display technique as a new antibody production method can express the protein on the minor coat of phage particle in a library constructed by utilizing a recombination of genes coding the variable regions of immunoglobulin. This new method is particularly advantageous in producing antibodies against toxic substances and compounds with low immunogenicities. We first confirmed the concept of antibody expression on the phage particle by selecting a positive control of the phage library (e.g., Griffin.l donated from MRC center in England). The library was then employed to produce antibodies specific to human serum albumin via repetitive bio-panning procedure. The mean affinity of the antibodies selected gradually increased along with the number of bio-panning, which demonstrated that the phage display method could produce monocloanl antibodies with high affinities.

• Archives of design research
• /
• v.11 no.2
• /
• pp.229-239
• /
• 1998

This study alms at developing the basic substance of education in Creating in 2- Dimentional Space, Which will make it possible to express and pursue creating which is appropriate for the characteristics of computer graphic as well as the technical side of the creating shape using computer in the established basic educational programs of the field, Thus in this study, I will put the concepts of creating shape relevant to various expression media in order. Based on this, I will clarify the potential of utilizing computer graphics and the extent to which they can applied, The purpose of this study lies 10 understanding computer as a means of creating and revealing the possibility for broadening the boundary of the establishd creating, For this, I am gOlOg to propose a plan for the education of Creating 10 2-Dimentional Space, limiting myself to the area relevant to computer graphics.

• Journal of Environmental Impact Assessment
• /
• v.2 no.2
• /
• pp.59-71
• /
• 1993

Evaluation methods are employed in environmental impact assessment to choose between different project site, to determine the required measures to compensate impact and to decide whether the environmental impacts are more important than the social or economic effects of a project. The main obstacles that restrict use of quantitative evaluation method are a Lack of knowledge about the environmental effects (e.g. if impacts on wildlife or landscape amenities are predicted) and the relative importance of economic and social issues compared with nature conservation stability of ecosystem or landscape beauty. In Germany, the most common method for site planning is the "ecological risk analysis". It is a kind of multi-criteria-decision-method based on quantitative and qualitative description and ordinal ranking. The various kinds of "ecological balancing methods" that are more recently developed (within the last decade) to quantify the required amount for compensatory measures instead often use cardinal figures to express the value of ecosystems, the intensity of impacts, the need for additional measures to compensate for long recuperative periods when restoring ecosystems and so on. There are still only a view attempts to quantify decisions between environmental and socio-economic issues. Multicriteria-analysis as well as cost-benifit-analysis was used. Some new approaches which are still in a preliminary status are based on contingent valuation and on calculations for compensatory payments (instead of compensatory measures).

• Proceedings of the KSAR Conference
• /
• 2004.06a
• /
• pp.228-228
• /
• 2004

Interferon-tau (IFN-τ) is the primary agent responsible for maternal recognition of pregnancy in cattle. Bovine embryos begine to express IFN-τ as the blastocyst forms. Pregnancy recognition in ruminants occurs when IFN-τ from the trophoblast prevents the increase of oxytocin receptors, disrupting luteolytic pulses of prostaglandin (PG) F2a by oxytocin. The expression of IFN-τ is strongly associated with the degree of methylation of the CpG islands in promoter region. (omitted)

• Journal of Microbiology and Biotechnology
• /
• v.6 no.6
• /
• pp.451-455
• /
• 1996

Physiological studies on the expression of Bacillus thuringiensis subsp. tenebrionis (Btt) gene coding for insecticidal protein in recombinant Escherichia coli 537 were carried out to identify optimal culture condition. It was necessary to shift culture temperature from 30 to $42^{\circ}C$ to express the gene. Expression of the Btt toxin gene by recombinant E. coli 537 began within one hour after induction. Complex nitrogen sources increased production of the insecticidal protein. The total insecticidal protein was 0.5 g/I when using yeast extract as a complex nitrogen source. Soybean hydrolysate showed apparently the highest induction efficiency. After induction, the cellular content of the insecticidal protein was 5.4 times higher than it had been before induction. The optimal cultivation strategy was found to grow cells for 7hours at $30^{\circ}C$ and then 5-8 hours at $42^{\circ}C$. The optimal cultivation pH for the production of insecticidal protein was 6.5. The Btt toxin produced by the recombinant E. coli 537 was found to have the same level of potency against Colorado potato beetle as the original toxin.

• Journal of Microbiology and Biotechnology
• /
• v.25 no.10
• /
• pp.1629-1633
• /
• 2015

Peptide assimilation in Helicobacter pylori necessitates a coordinated working of the peptide transport systems (PepTs) and aminopeptidase (PepA). We found that H. pylori hydrolyzes two detector peptides, _L-phenylalanyl- _L-3-thiaphenylalanine (PSP) and _L-phenylalanyl- _L-2-sulfanilylglycine (PSG), primarily before intake and excludes their antibacterial effects, whereas Escherichia coli readily transports them with resultant growth inhibition. PSP assimilation by H. pylori was inhibited by aminopeptidase inhibitor bestatin, but not by dialanine or cyanide-m-chlorophenylhydrazone, contrary to that of E. coli. RT- and qRT-PCR analyses showed that H. pylori may express first the PepTs (e.g., DppA and DppB) and then PepA. In addition, western blot analysis of PepA suggested that the bacterium secretes PepA in response to specific inducers.

• The Journal of the Korea Contents Association
• /
• v.14 no.12
• /
• pp.574-585
• /
• 2014

Filming of movie is not working for translating scenario of story or character's moving. Director of Photography accumulates emotion in scenario through image. The accumulation of continuation is the essence of movie. Therefore, cinematographer strives consistently to analysis scenario and to express through visual which operation of the most basis can be filming technology. Eventually, Filming of movie is based on mean of technology to show artistry work. Accordingly, searching the most fundamental of filming's technical factor considerably help to understand movie art. There are variation technology factors to expose character's emotion. And the most simple and influential try by only using the pure function of camera is utilizing shutter without supporting lighting and production design. As a result, this thesis discuss about essential and basic factors of utilizing shutter.

• Journal of Multimedia Information System
• /
• v.4 no.4
• /
• pp.255-262
• /
• 2017

In recent software development, repetitive code fragments (i.e., clones) are common due to the copy-and-paste programming practice, the framework-based development, or the reuse of same design patterns. Such similar code fragments are likely to introduce more bugs but are easily disregarded by a code reviewer or a programmer. In this paper, we present a code review tool to help code reviewers identify important code changes written by other programmers and recommend which changes need to be reviewed first. Specifically, to identify important code changes, our approach detects code clones across revisions and investigates them. Then, to help a code reviewer, our approach ranks the identified changes in accordance with several software quality metrics and statistics on those clones and changes. Furthermore, our approach allows the code reviewer to express their preferences during code review time. As a result, the code reviewer who has little knowledge of a code base can reduce his or her effort by reviewing the most significant changes that require an instant attention. To evaluate our approach, we integrated our approach with a modern IDE (e.g., Eclipse) as a plugin and then analyzed two third-party open source projects. The experimental results indicate that our approach can improve code reviewer's productivity.

• Journal of the Korean Chemical Society
• /
• v.3 no.1
• /
• pp.23-27
• /
• 1954

The relation between the height of ascending solvents and its velocity were studied with strips of Toyo No. 50 filter paper. The filter paper strip of 3cm width and cat 30 cm length is hooked down or fixed between two sheets of glass plates inside a cylinder containing the solvent, in which the lower end of the strip is dipped. As the solvents, acetone, hydrochloric acid, water or mixtures of these are used. For short time intervals, Ostwald's folmula $h = Kt^n$ was found to be most suitable to express the relation between the height of the solvent front, h, and time, t. For longer time intervals, results will be discussed in the next paper. The mean values of the constant m in the above formula for pure acetone and pure water were 0.44 and 0.485, respectively, and that of K were 0.05 and 2.4, respectively. The time interval whose the above formula applies, for each solvent was 2 hours for the former, and a half hour for the later, respectively. The movement of solutes, such as $Cu^{++}$ and $Fe^{++}$ showed that the Rf values of solutes become constant values after a long periods; e.g., ca. 6 hours with 10N-HCl-Acetone (1 : 9) solvent.

• Journal of the Korean Society of Costume
• /
• v.59 no.9
• /
• pp.131-145
• /
• 2009

Literature in beauty art is insufficient regarding beauty spot, though it has played an important role in beauty art history. This study aims at analyzing various patterns and decorative functions of beauty spots and examining their remarkable differences by place and time. It attempts to find the symbolistic characteristics of beauty patch which influenced European countries socially and culturally in the 17th and 18th centuries, findings are as follows: first, beauty spots are extremely various by patch patterns and by forms, e.g. Wha-Jeon(花鈿), Bindi and artificial points. In the view of cosmetic purpose, they have been used to beautify appearances, reveal social positions and express the desires like blessing or protection from evil. In the times of Baroque and Rococo, though temporarily, people with beauty spots and their belonged societies used them as communicative tools: obviously, they functioned symbolistically. This study surveyed 156 persons to find what cosmetic messages and images beauty spots present in the contemporary world. Using SPSS 13.0, the study analyzed the responses and found that beauty spots are still functioning and welcomed to let women look more beautiful and more attractive, though their cosmetic messages and sizes have been simplified and scaled down.