• Title/Summary/Keyword: Experiment study

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In Vitro Cytotoxicity, Skin Regeneration, Anti-wrinkle, Whitening and In Vivo Skin Moisturizing Effects of Oncheongeum (온청음 물 추출물의 세포독성, 피부재생, 주름개선, 미백 및 보습 효과)

  • An, Tteul-E-Bom;Kim, Dong-Chul
    • The Journal of Korean Obstetrics and Gynecology
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    • v.29 no.1
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    • pp.14-34
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    • 2016
  • Objectives: The objective of this study was to evaluate the effects of cytotoxicity, skin regeneration, anti-wrinkle, whitening and skin moisturizing of Oncheongeum (OCE).Methods: The cytotoxicity of OCE lyophilized aqueous extracts (yield=13.82%) was observed against human normal fibroblast cells and B16/F10 murine melanoma cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium Bromide (MTT) assay, and skin regeneration and anti-wrinkle effects were also evaluated through the assay of collagen type I synthesis compared to the transformation of the growth factor (TGF)-β1, hyaluronidase, collagenase and matrix metalloproteinase (MMP)-1 inhibitory assays compared to oleanolic acid (OA), and elastase inhibitory effects compared to phosphoramidon disodium salt (PP). In addition, OCE’s whitening effects were measured by a tyrosinase inhibitory assay and melanin formation test in B16/F10 murine melanoma cells compared to arbutin, and skin moisturizing effects were observed through a mouse skin water content test, respectively. Results: No OCE treatment-related cytotoxic effects appeared on human normal fibroblasts and B16/F10 murine melanoma cells. OCE concentration-dependently increased the collagen Type I synthesis on human normal fibroblast cells, and also effectively inhibited hyaluronidase, elastase, collagenase and MMP-1 activities. In addition, OCE inhibited melanin production of B16/F10 murine melanoma cells and activity of tyrosinase. And significant and dose-dependent increases of skin water content were detected in OCE-treated mouse skin compared to vehicle control skins. Conclusions: OCE showed favorable and sufficient effects in skin regeneration, anti-wrinkle, whitening and skin moisturizing in this experiment. But more detail mechanisms and studies on the skin protective efficiency of in vivo are needed with the screening of active biological compounds in individual OCE herbs.

Effects of Vitamin C on Residual Aflatoxin $B_1$ in Rat Sera Treated with Radiation and Aflatoxin $B_1$ (Vitamin C가 방사선과 Aflatoxin $B_1$을 투여한 흰쥐의 혈청 중 Aflatoxin $B_1$ 잔류량에 미치는 영향)

  • Chung, Do-Young;Kim, Han-Soo;Kang, Jin-Soon
    • Food Science and Preservation
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    • v.18 no.3
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    • pp.374-382
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    • 2011
  • Aflatoxin ($AFB_1$) is a potent hepatotoxic and hepatocarcinogenic mycotoxin in humans. It is also well-known to be accumulated in animal tissues via various metabolic pathways. This study was conducted to determine the effects of vitamin C on the residual $AFB_1$ in rat sera that were treated with radiation and $AFB_1$. Six week-old male Sprague-Dawley rats were randomly divided into five groups: a control group, $AFB_1$-treated group, the group treated with $AFB_1$ and vitamin C, the group treated with X-ray and AFB1, and the group treated with X-ray and $AFB_1$ with vitamin C. On the first day of the experiment, only one dose of X-rays was exposed to the entire liver at 1,500 cGy. Next, vitamin C was injected at 10 mg/kg body weight via intraperitoneal injection, followed 1 hr later by the administration of 0.4 mg/kg of $AFB_1$ via intraperitoneal injection. These treatments were then administered every three days over a period of 15 days. On the 16th day of treatments, the animals were sacrificed. The contents of $AFB_1$ in rat sera were determined via indirect competitive ELISA and HPLC method. In the quantitative analysis of $AFB_1$ in rat sera via ELISA, $5.17{\pm}0.34$ ng/mL of $AFB_1$ was detected in the $AFB_1$-treated groups, but the amount more significantly decreased to $3.23{\pm}0.76$ ng/mL in the groups treated with $AFB_1$ and vitamin C (p<0.01) than in the $AFB_1$-treated groups. The $AFB_1$ contents of the rat sera of the groups treated with X-ray and $AFB_1$ did not significantly decreased with the administration of vitamin C. The $AFB_1$ content of the rat sera that was analyzed via HPLC showed a tendency similar to that of the content that was analyzed via ELISA. With regard to these data, vitamin C was very effective in reducing $AFB_1$ residue in rat sera.

Effect of Packaging Systems with High CO2 Treatment on the Quality Changes of Fig (Ficus carica L) during Storage (저장 중 무화과(Ficus carica L) 선도유지를 위한 고농도 이산화탄소 처리된 포장 시스템 적용 연구)

  • Kim, Jung-Soo;Chung, Dae-Sung;Lee, Youn Suk
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.799-806
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    • 2012
  • This experiment was conducted to establish the optimum conditions for high $CO_2$ gas treatment in combination with a proper gas-permeable packaging film to maintain the quality of fig fruit (Ficus carica L). Among the fig fruits with different high $CO_2$ treatments, the quality change was most effectively controlled during storage in the 70%-$CO_2$-treated fig fruit. Harvested fig fruit was packaged using microperforated oriented polypropylene (MP) film to maintain the optimum gas concentrations in the headspace of packaging for the modified-atmosphere system. MP film had an oxygen transmission rate of about $10,295cm^3/m^2$/day/atm at $25^{\circ}C$. The weight loss, firmness, soluble-solid content (SSC), acidity (pH), skin color (Hunter L, a, b), and decay ratio of the fig fruits were monitored during storage at 5 and $25^{\circ}C$. The results of this study showed that the OPP film, OPP film + 70% $CO_2$, and MP film+70% $CO_2$ were highly effective in reducing the loss rate, firmness and decay occurrence rate of fig fruits that were packaged with them during storage. In the case of using treatments with packages of OPP film and OPP film+70% $CO_2$, however, adverse effects like package bursting or physiological injury of the fig may occur due to the gas pressure or long exposure to $CO_2$. Therefore, the results indicated that MP film containing 70% $CO_2$ can be used as an effective treatment to extend the freshness of fig fruits for storage at a proper low temperature.

Seed Dormancy Type and Germination Characteristics in Tiarella polyphylla D. Don Native to Korea (한반도 자생식물 헐떡이풀 종자의 휴면유형과 발아특성)

  • Choi, Han;Lee, Seung Youn;Rhie, Yong Ha;Lee, Jeong Ho;Kim, Sang Yong;Lee, Ki Cheol
    • Korean Journal of Plant Resources
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    • v.31 no.4
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    • pp.363-371
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    • 2018
  • Tiarella polyphylla D. Don is a native plant distributed only in Ulleung Island in Korea and has been traditionally used for medicinal purposes, although it is also used ornamentally. This study was conducted to determine the requirements for dormancy break and germination and to classify the type of seed dormancy. The experiments were performed with cold stratification (0 or 12 weeks at $5^{\circ}C$), warm stratification (0, 4, 8, or 12 weeks at $23^{\circ}C$, followed by 8 weeks at $5^{\circ}C$, and then incubation at $23^{\circ}C$), and $GA_3$ treatments (0, 10, 100, or 1000 mg/L). The treated seeds were incubated on aseptic media at room chamber ($23^{\circ}C$, a 16h photoperiod of fluorescent lamps with $40{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$). The seeds were dispersed in nature as underdeveloped embryos with no physical barrier to absorb water to prevent water absorption. However, the seeds did not germinate for 30 days after sowing without any pre-treatments. Thus, the seeds had morphological dormancy (MD) and physiological dormancy (PD). The final germination percentage following cold stratification (0 or 12 weeks) was 66.7% and 45.6%, respectively. The cold stratification delayed seed germination by about 3 weeks. In the warm stratification experiment (0, 4, 8, or 12 weeks), the final germination percentage was 21.1%, 27.8%, 41.1%, and 57.8%, respectively, 20 weeks after sowing. The embryos of the T. polyphylla seed grew in relatively warm temperatures ($23^{\circ}C$). $GA_3$ application overcame seed dormancy and promoted germination. Following $GA_3$ treatment (0, 10, 100, or 1000 mg/L), the final germination percentage was 33.3%, 45.0%, 42.5%, and 72.5%, respectively. These results suggest that the T. polyphylla seeds had non-deep simple morphophysiological dormancy (MPD) and $GA_3$ treatment could be used as a substitute for warm stratification for breaking seed dormancy. To our knowledge, this is the first report of seed dormancy characteristics of the genus Tiarella native to Korea.

Development and Application of Scientific Model Co-construction Program about Image Formation by Convex Lens (볼록렌즈가 상을 만드는 원리에 대한 과학적 모형의 사회적 구성 프로그램 개발 및 적용)

  • Park, Jeongwoo
    • Korean Journal of Optics and Photonics
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    • v.28 no.5
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    • pp.203-212
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    • 2017
  • A scientific model refers to a conceptual system that can describe, explain, and predict a particular physical phenomenon. The co-construction of the scientific model is attracting attention as a new teaching and learning strategy in the field of science education and various studies. The evaluation and modification of models compared with the predicted models of data from the real world is the core of modeling strategy. However, there were only a limited data provided by the teacher in many studies of modeling comparing the students' predictions of their own models. Most of the students were not given the opportunity to evaluate the suitability of the model with the data in the real world. The purpose of this study was to develop a scientific model co-construction program that can evaluate the model by directly comparing the predicted models with the observed data from the real world. Through a collaborative discussion between teachers and researchers for 6 months, a 5-session scientific model co-construction program on the subject 'image formation by convex lenses' for second grade middle school students was developed. Eighty (80) students in 3 classes and a science teacher with 20 years of service from general public co-educational middle school in Gyeonggi-do participated in this 2-week program. After the class, students were asked about the helpfulness and difficulty of the class, and whether they would like to recommend this class to a friend. After the class, 95.8% of the students constructed the scientific model more than the model using the construction rule. Students had difficulties to identify principles or understand their friends, but the result showed that they could understand through model evaluation experiment. 92.5% of the students said that they would be more than willing to recommend this program to their friends. It is expected that the developed program will be applied to the school and contribute to the improvement of students' modeling ability and co-construction ability.

Evaluation of Surface Covering Methods for Reducing Soil Loss of Highland Slope in Radish Cultivation (고랭지 경사 밭 무 재배지에서 토양유실경감을 위한 피복방법 평가)

  • Lee, Jeong-Tae;Lee, Gye-Jun;Ryu, Jong-Soo;Park, Suk-Hoo;Han, Kyung-Hwa;Zhang, Yong-Seon
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.667-673
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    • 2011
  • There is relatively high vulnerability of soil erosion in slope highland agriculture due to a reclamation of mountain as well as low surface covering in early summer season with high rainfall intensity time. The aim of this study was to evaluate various surface covering methods for reducing soil loss in highland radish cultivation in highland. The experiment was conducted in 17% sloped lysimeter ($2.5m{\times}13.4m$) with 8 treatments including covering with cut rye, sod culture of rye, Ligularia fischeri var. spiciformis Nakai, Arachniodes aristata Tindale, Aster koraiensis Nakai, Festuca myuros L. and mulching with black polyethylene film, and runoff water, eroded soil and radish growth were investigated. Surface covering with sod culture and plant residue, especially cut rye treatment, had lower runoff water than non-covering, whereas black polyethylene film mulching had the reverse. The amount of eroded soil was also lowest in cut rye treatment, $0.3Mg\;ha^{-1}$, and increased in the order of rye sod culture, Ligularia fischeri var. spiciformis Nakai, Aster koraiensis Nakai, Festuca myuros L., Arachniodes aristata Tindale, black polyethylene film, and non-covering, $68.2Mg\;ha^{-1}$. The results showed that surface covering with sod culture or plant residue could be effective for reducing runoff water and soil erosion in the radish field, significantly in cut rye treatment. On the other hand, in sod culture of rye, Aster koraiensis Nakai and Ligularia fischeri var. spiciformis Nakai, radish yields were lower than in the non-covering. Unlike this, covering with cut rye, sod culture of Festuca myuros L. had similar radish yield to the non-covering radish yield. In conclusion, covering with cut rye and sod culture of Festuca myuros L. were beneficial for reduction of soil loss without decreasing in radish yield in highland sloped fields.

The Effect of Application Levels of Slurry Composting and Bio-filtration Liquid Fertilizer on Soil Chemical Properties and Growth of Radish and Corn (총각무와 옥수수 재배시 SCB액비 시용수준이 토양화학성과 생육에 미치는 영향)

  • Kang, Seong-Soo;Kim, Min-Kyeong;Kwon, Soon-Ik;Kim, Myong-Suk;Yoon, Sung-Won;Ha, Sang-Gun;Kim, Yoo-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1306-1313
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    • 2011
  • A liquid fertilizer treated with slurry composting and biofiltration (SCB) process has been applied increasingly on agricultural field but the effects on the soil properties and crop production has not been throughly evaluated. This study was conducted to investigate the effect of the SCB application on soil chemical properties and the growth of radish and corn. SCB liquid fertilizer as a basal fertilization was treated with five levels based on $6kg\;10a^{-1}$ for radish and $10kg\;10a^{-1}$ for corn. The experimental design was the completely randomized block design with five levels and three replicates. Electrical conductivity (EC), $NO_3$-N, Exch. K and Exch. Na increased depending on the treatment levels of SCB. There were no changes in soil organic matter, Avail. $P_2O_5$, Exch. Ca and Exch. Mg. EC, $NO_3$-N and Exch. Na content decreased as precipitation increased. Especially, they decreased up to the initial condition before the treatment after the heavy rainy season in 2008. Although Exch. K decreased at the rainy season, they remained relatively higher content after the experiment on August, 2008. Fresh weight and the amount of N uptake of radish increased due to the levels of SCB, but corn did not present any significant increase. It is recommended that we need to decide the proper amount of SCB as well as the application method on the field to increase the productivity and decrease environmental stress. Additional experiments also need to clarify the effect of the trace element and heavy metal accumulations due to long term application of SCB.

Effect of Monoculture and Mixtures of Green Manure Crimson Clover (Trifolium incarnatum) on Rice Growth and Yield in Paddy (답리작에서 녹비작물 크림손클로버 단파 및 혼파가 벼 생육 및 수량에 미치는 영향)

  • Jeon, Weon-Tai;Seong, Ki-Yeong;Kim, Min-Tae;Oh, In-Seok;Choi, Bong-Su;Kang, Ui-Gum
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.5
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    • pp.847-852
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    • 2011
  • Green manure crops are mainly used to reduce the application of chemical fertilizers. Mixture of green manure crops have beneficial effects in agroecosystem. In this study, experiments were conducted to evaluate the effects of monoculture and mixtures of crimson clover (Trifolium incarnatum) on rice growth and yield in paddy. This experiment was conducted at Sinheung series (fine loamy, mixed, nonacid, mesic family of Fluvaquentic Endoaquepts) from Oct. 2007 to Oct. 2009 at the National Institute of Crop Science (NICS), RDA, Suwon, Gyeonggi province, Korea. Seeding rates of crimson clover (CC) were consisted of monoculture (CC2, 3, 4 kg and hairy vetch 5 kg $10a^{-1}$) and mixtures (CC 2 + barley 7, CC 3 + barley 7, CC 4 + barley 7, and CC2 + hairy vetch $5kg\;10a^{-1}$). Seeds were drilled by partial tillage machine on 9th Oct. in 2007. Monoculture and mixture of crimson clover as a green manure crop was incorporated in soil for rice cultivation on 15th May in 2008. Chemical fertilizers had not been applied to monoculture and mixture plots. The biomass and N production of monoculture plots were lower than mixture plots. The biomass and N production of CC 2 + hairy vetch $5kg\;10a^{-1}$ plot were the highest among mixtures treatments. In rice growing season, ammonium nitrogen concentrations in soil were a little high trends at CC 2 + hairy vetch $5kg\;10a^{-1}$ plot. And soil bulk density and porosity were improved at mixture plot after rice harvesting. The rice yield of CC 2 + hairy vetch $5kg\;10a^{-1}$ plot was not significantly different from conventional practice plot. These results indicated that cropping of crimson clover with hairy vetch mixture was better than barley mixture for environmental friendly rice cultivation.

Effect of Green Manure Crops Incorporation with Rice Cultivation on Soil Fertility Improvement in Paddy Field (벼 재배시 녹비작물 혼입에 따른 지력개선 효과)

  • Yang, Chang-Hyu;Ryu, Jin-Hee;Kim, Taek-Kyum;Lee, Sang-Bog;Kim, Jae-Duk;Baek, Nam-Hyun;Kim, Sun;Choi, Weon-Young;Kim, Si-Ju
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.5
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    • pp.371-378
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    • 2009
  • This study was carried out to investigate the improvement effect of soil fertility by incorporation of GMC(green manure crops) at rice cropping after cultivation GMC such as the barley for alternative rye in paddy field over the past two years(2006~2007). Plots, which consisted of incorporation time of GMC as rye; heading stage, barley; heading stage, heading stage of rye and 10days after heading stage were divided by amount of applied rates; standard fertilizer fertilization, diagnosis fertilization and non-fertilization. we investigated change of soil physico-chemical properties, degree of decomposition on GMC in soil, growth and yield potential. The obtained results were summarized as follows. The fresh weight of GMC at incorporation time on heading stage of rye, heading stage and 10days after heading stage of barley were $2,715,\;2,352,\;2,867kg\;10a^{-1}$ respectively. Content of total nitrogen at three incorporation times was 1.31, 1.46, 1.38% and the C/N ratio were 33.4, 28.7, and 34.6, respectively. Some soil physical properties, such as soil hardness and bulk density tended to decrease with incorporation of GMC, while surface soil depth and porosity were increased. Some soil chemical properties, such as content of exchangeable cations and cation exchangeable capasity(CEC) were increased with incorporation of GMC compared with before experiment. Rice yields was increased 3~9% in diagnosis application plots on application of barley compared with control($559kg\;10a^{-1}$) and incorporation of barley caused to improve perfect kernel ratio 73.6~78.7% in appearance characteristics of brown rice compared with cotrol(73.0%). It was found that incorporation with 10days after heading stage of barley was more effective to reduce chemical nitrogen fertilizer and to improve soil fertility.

Environmental Impacts of Food Waste Compost Application on Paddy Soil (음식물쓰레기 퇴비 시용이 논토양에 미치는 영향)

  • So, Kyu-Ho;Seong, Ki-Seog;Seo, Myung-Chul;Hong, Seung-Gil
    • Korean Journal of Soil Science and Fertilizer
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    • v.40 no.1
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    • pp.85-94
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    • 2007
  • To determine the influence of food waste compost (FWC) application on paddy soil, FWC was applied to the paddy soil and then compared with farmer's practice as to the effects on rice and soil environment. Initially, pig manure compost (PMC) had high content of phosphorus ($15g\;kg^{-1}$) and potassium ($23g\;kg^{-1}$), while FWC had high content of total nitrogen ($13g\;kg^{-1}$) and salinity ($18.5g\;kg^{-1}$). Comparison was also made between chemical fertilizer and FWC use as a trial in the paddy field under the clay loam and sandy loam soil. In the panicle formation stage, chemical fertilizer application was proper in clay loam while PMC application was proper in sandy loam. However, chemical fertilizer produced higher yield compared to compost treatment, both on clay loam and sandy loam with 20~25% and 17~19%, respectively. The lower yield in sandy loam maybe due to slow mineralization of compost such that the crop did not effectively use it. Organic matter content in paddy soil after experiment was higher in FWC and PMC plots compared to that in chemical fertilizer plots. But the other soil properties were comparable. Therefore, the FWC compost had little effect on soil when it use as a trial in paddy field. Likewise, after the application of FWC as a trial, analysis of nitrate nitrogen and ammonium nitrogen in the surface water and 60 cm depth of paddy soil water nine days after planting was done. Results revealed that concentration of ammonium nitrogen was similar to irrigation water while nitrate nitrogen concentration was not detected, and hence did not contribute to water pollution. It is concluded that the application of FWC in the paddy field had not affected on environmental pollution in the paddy field. But its use as compost during rice culture reduced yield quantity. Such study should include selection of compost material, amount and method of compost application.