• Title/Summary/Keyword: Experiment study

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Genetic Diversity of Korean Rice Breeding Parents as Measured by DNA Fingerprinting with Simple Sequence Repeat (SSR) Markers

  • Song, Moon-Tae;Lee, Jeom-Ho;Lee, Sang-Bok;Cho, Youn-Sang;Ku, Ja-hwan;Seo, Kyoung-In;Choi, Seong-ho;Hwang, Heung-Goo
    • Plant Resources
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    • v.6 no.1
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    • pp.16-26
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    • 2003
  • Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

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The Effect of Microcurrent Stimulation on Immediately Early Gene in Pain Induced Model (실험적 통증유발 모델에서 조기발현 유전자에 대한 미세전류자극의 효과)

  • Kim Gye-Yeop;Kim Tae-Youl;Oh Myung-Hwa;Kim Sun-Eun;Cheong Mee-Sun;Suh Young-Sook
    • The Journal of Korean Physical Therapy
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    • v.16 no.3
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    • pp.9-21
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    • 2004
  • The purpose of study is that we will observe the change of c-fos with the immunohistochemistry method and then we will study the effect of micro current stimulation following the frequency after inducing pain to rats with capsaicin. Rat were classified to SD and they have growed for 8 weeks. We classify to four groups, ordinal group is used in experiment I, the group which we induce pain is used in experiment II, the application group which we induce pain and then the high frequency micro current stimulation is used in experiment III, the application group which we induce pain and then the low frequency micro current stimulation is used in experiment IV, we get the following result. Compare with experiment II, experiment III, and experiment IV from acute pain modal in the immunohistochemistry experiment which has c-fos protein as an antigen, c-fos immunoreactive positive neurons significantly after induced pain for two hours(p<0.001). According to these results, from rats induced pain, micro current stimulation effect to reducing pain, but following frequency micro current stimulation theraphy isn't different from immunoreactive c-fos

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Evaluation of QTL Related SSR Marker Universality in Korean Rice Breeding Populations

  • Song, Moon-Tae;Lee, Jeom-Ho;Lee, Sang-Bok;Ku, Ja-Hwan;Cho, Youn-Sang;Song, Myung-Hee;Park, Sung-Ho;Hwang, Hung-Goo
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.1
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    • pp.56-64
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    • 2003
  • If a quantitative trait loci (QTL) marker identified in a population is applicable to different populations (marker universality), this will not only reduce the labor and cost in marker assisted selection (MAS), but accelerate the application of molecular markers to real breeding programs. Present study aims to evaluate the defined QTL related markers from a population to a different breeding population for the MAS. Four rice breeding populations were subjected to seventy-five simple sequence repeat (SSR) markers which were already identified for their polymorphism information content (PIC) in the parents of the crossings. Among them, eight markers were evaluated for their correlation between presence of marker alleles and phenotypic expression in breeding populations. A reasonable level of polymorphism for the mapped markers originated from any sources of rice accessions was observed between crosses of any sources (marker repeatability). However, correlation between presence of markers and expression of the traits in rice breeding populations was not significant except for minor portion of traits and markers examined (failure of marker universality). In the present study, various strategies were discussed to develop new markers with universality of breeding application.

A Study of High School Students' and Science Teachers' Understanding of Ideal Conditions Involved in the Theoretical Explanation and Experiment in Physics: Part IV- Focused on the Ideal Condition Involved in the Experiment - (물리학에서 이론적 설명과 실험에 포함된 이상조건에 대한 고등학생과 과학교사의 이해 조사 IV-실험에 포함된 이상조건을 중심으로-)

  • Park, Jong-Won;Chung, Byung-Hoon;Kwon, Sung-Gi;Song, Jin-Woong
    • Journal of The Korean Association For Science Education
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    • v.19 no.1
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    • pp.78-90
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    • 1999
  • This study investigated the high school students' and science teachers' understanding of idealization involved in the physics experiment. Major research questions are as follows: (1) what kind of ideal conditions do subjects identify from the presented experimental context? (2) do subjects think how well ideal conditions are satisfied with the experiment? (3) how well do subjects expect the effect of idealization on the experimental result? (3) what kind of view point do science teachers have about the ideal condition involved in the experiment? A total of 85 subjects were given 6 questions related with the research questions I. 2. and 3. with simple experiment about the brightness of the bulbs connected with parallel to the battery. And another 4 questions for the forth research question were given to 42 science teachers. Subjects' responses were summarized and used to draw the implications for the teaching of physics experiment.

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A Study about Voice of Patients with Chronic Obstructive Pulmonary Disease/Asthma before & after ${\beta}_2$-agonist (${\beta}_2$-촉진제 사용전후에 따른 만성폐쇄성폐질환/천식 환자의 음성 연구)

  • Kang, Young-Ae;Kim, Se-Hun;Jong, Seong-Su;Lee, Tae-Yong;Seong, Cheol-Jae
    • Phonetics and Speech Sciences
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    • v.2 no.2
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    • pp.101-108
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    • 2010
  • An inhaled salbutamol and salmeterol for chronic obstructive pulmonary disease(COPD) and asthma have been used worldwidely. But there has been few study about the voice change evoked from the post-medicine effect. To evaluate the voice influenced of short-acting and long-acting ${\beta}_2$-agonists, two experiments were carried out: one was salbutamol experiment 1 with eight patients, the other was salmeterol experiment 2 with six patients. Experiment 1 was made of two stages: premedication & postmedication. Experiment 2 was four stages: stageI was premedication, stageII was postmedication & pregaggling, stageIII was postmedication & postgaggling(100 ml with water), and stageIV was postmedication & 30 minutes later. Measured parameters were F0, F0_SD, Jitter_rap, Shimmer_apq11, HNR, BW(1, 2, 3), Intensity, and H1-H2. The mean data collected from 3 repetitions each was statistically analyzed by Wilcoxon signed rank test for experiment 1 and repeated measures ANOVA for experiment 2. In experiment 1, significant differences were found in the Jitter_rap(Z= -2.10, p=0.036). The findings indicated that the postmedicated voice was worse than premedicated voice. In experiment 2, there wasn't significant difference, but values of parameters related to voice quality(Jitter_rap, Shimmer_apq11, HNR, and H1-H2) showed changes toward stageⅣ, that is, the voice quality was worse under medication.

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Effects of Meteorological Conditions on Cloud and Snowfall Simulations in the Yeongdong Region: A Case Study Based on Ideal Experiments (영동지역 기상조건이 구름 및 강설 모의에 미치는 영향: 이상 실험 기반의 사례 연구)

  • Kim, Yoo-Jun;Ahn, Bo-Yeong;Kim, Baek-Jo;Kim, Seungbum
    • Atmosphere
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    • v.31 no.4
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    • pp.445-459
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    • 2021
  • This study uses a cloud-resolving storm simulator (CReSS) to understand the individual effect of determinant meteorological factors on snowfall characteristics in the Yeongdong region based on the rawinsonde soundings for two snowfall cases that occurred on 23 February (Episode 1) and 13 December (Episode 2) 2016; one has a single-layered cloud and the other has two-layered cloud structure. The observed cloud and precipitation (snow crystal) features were well represented by a CReSS model. The first ideal experiment with a decrease in low-level temperature for Episode 1 indicates that total precipitation amount was decreased by 19% (26~27% in graupel and 53~67% in snow) compared with the control experiment. In the ideal experiment that the upper-level wind direction was changed from westerly to easterly, although total precipitation was decreased for Episode 1, precipitation was intensified over the southwestern side (specifically in terrain experiment) of the sounding point (128.855°E, 37.805°N). In contrast, the precipitation for Episode 2 was increased by 2.3 times greater than the control experiment under terrain condition. The experimental results imply that the low-level temperature and upper-level dynamics could change the location and characteristics of precipitation in the Yeongdong region. However, the difference in precipitation between the single-layered experiment and control (two-layered) experiment for Episode 2 was negligible to attribute it to the effect of upper-level cloud. The current results could be used for the development of guidance of snowfall forecast in this region.

Verification on Debris Reduction Ability of the Sweeper by Real Scale Experiment (실규모 실험검증을 통한 스위퍼의 유송잡물 저감능력 검토)

  • Kim, Sung-Joong;Jung, Do-Joon;Kang, Joon-Gu;Yeo, Hong-Koo;Kim, Jong-Tae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.9
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    • pp.34-44
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    • 2016
  • This study is an experimental study about a facility for preventing the accumulation of floating debris at a bridge by flooding at a small river. Generally, structures installed at a small river are damaged frequently by floating debris during typhoons or localized rainfall events. On the other hand, there is no method available for preventing such damage. The facilities used in other countries to prevent such damage by the accumulation of floating debris include debris fins, deflectors, and sweeper. Among these facilities, the present study was conducted with a sweeper to investigate the damage-reducing capability through a real-scale accumulation experiment. A sweeper was installed in front of a bridge to bypass floating debris by self-rotation so that the floating debris may not be accumulate at the bridge. A small bridge model was prepared in a real-scale for the real-scale experiment. The accumulation reducing capability was compared through an accumulation experiment before and after the sweeper installation depending on the length of the debris and flow conditions. The result showed that the accumulation rate increased with increasing length of the debris or decreasing flow rate. The installation of a sweeper decreased the debris accumulation rate by a minimum of 55% to a maximum of 88% compared to the case without an installed sweeper. The result of the present study showed that the installation of a sweeper at a small river having a high potential of generating floating debris may help secure the stability of a bridge in the case of floating debris accumulation.

화재감지기의 경년도에 상관한 감응특성 실험.연구

  • Lee, Bok-Yeong;Yu, In-Ho
    • Fire Protection Technology
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    • s.16
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    • pp.13-19
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    • 1994
  • An Experimental study on the relation between responsiveness and elapse of years This experiment study is to appraise a fire detector's durability & reliability. Fire detector is located in office, temp range 10-27 고 65 15%, installed in the ceiling with power on. This is the experiment report of elapsed 5 years.

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AN EXPERIMENTAL STUDY OF MANDIBULAR FRACTURE WOUND HEALING IN THE CALCIUM DEFICIENT RAT (저칼슘식이가 백서의 하악골 골절치유에 미치는 영향에 관한 실험적 연구)

  • LEE Sang-Hoon;HWANG Eui-Hwan;LEE Sang-Rae
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.27 no.1
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    • pp.123-140
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    • 1997
  • The purpose of this study was to investigate effects of osteoporosis on fracture wound healing in the calcium deficient rat. To research the experiment some ten-week old Wistar strain rats with approximately 300 gms weight were selected. Then, the rats were divided into two groups: Normal diet group(rats given a normal diet before and after bone fracture) and Low calcium diet group(rats given a low calcium diet before and after bone fracture). Both groups had been provided with each diet for three weeks. When the rats became thirteen weeks old, the mandibular angle of rats in both groups was artificially fractured for test. The healing of fracture wounds was reviewed by using soft x-ray radiography and /sup 99m/Tc-MDP bone scan and also histopathologic examination. The obtained results were as follows : 1. The radiolucency of the fracture site for the Normal diet group started to decrease from the 14th day since the experiment was made, while the Low calcium diet group began decrease in the radiolucency from the 21st day of the experiment. The radiolucency for the Normal diet group disappeared at the 42nd day, but one for the Low calcium diet group disappeared at the 56th day of the experiment. 2. The highest uptake rate of /sup 99m/Tc-MDP stood at the 14th day of the experiment in the Normal diet group and the Low calcium diet group's maximum rate was recorded at the 21st day of the experiment. These both groups were gradually experiencing decrease in the uptake rate as the experiment time was going on. However, the uptake rate in the Low calcium diet group was lower than one in the Normal diet group. 3. For the Normal diet group, the newly formed trabeculae, which were similar to one of the surroundung bone, were seen at the 42nd day of the experiment. On the other hand, the Low calcium diet group showed at the 56th day of the experiment that the osteoporotic findings looked weak, irregular trabeculae, and also large bone marrow space were observed clearly. As a result of the above experiment, it is said that the healing of the fracture can be completed for both groups, the Normal diet group and the Low calcium diet group. However, the amount of the newly formed bone wound in the Low calcium diet group is rather decreased compared to one in the Normal diet group and at the same time the healing of the fracture is delayed in the Low calcium diet group. Consequently, for the successful healing of fracture in osteoporosis, it is considered that the management of the etiologic factors of osteoporosis must be preceded. The more study of calcium metabolism and functions of osteoblast and osteoclast needs to proceed on.

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Comparison of Motor Fitness of Cerebral Palsy Chidren with normal throug Phyisical Fitness Diagnosis Evaluation (체력진단 평가를 통한 뇌성마비 아동과 정상아동의 운동능력 비교연구)

  • Lee Kang-Jun;Park Rae-Joon;Kim Jong-Yul
    • The Journal of Korean Physical Therapy
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    • v.12 no.1
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    • pp.101-112
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    • 2000
  • The aim of this study is to compared the cerebral palsy children with normal children in the exercise and cardio vascular ability after this study as the fundamental data fer mating programs for the cerebral palsy children. The test of this study is about twenty girls, the control group of the normal children(n=10) and the experiment group of cerebral palsy children(n=10). They were studied in four aspects which were the anthropometry, the medical check in the rest, the physical fitness or exercise roads test and the change of the target heart rate during exercise. The result were as follows : 1. The characteristic of the physical type : The control group is higher than the experiment group in the standing height and the body weight but their's little difference between them (p<0.05). The control group is lower than the experiment group in the body fat. 2. The characteristic of the medical check in the rest : The control group is higher than the experiment group in the vital capacity and flood expiratoryvolume one second. The control group is higher that the experiment group in the blood pressure of systolic and Diastolic. There's little difference between them(p<0.01). The control is lower than the experiment group in the heart pulse rate. There's little difference between them(p<0.005). 3. The characteristic of basic physical strength evaluation : The experiment group is the lowest dynamic muscular endurance, balance, agility and endurance which need to move the body with weight. The control group is much higher than the experiment group in the flexibility and muscle strength(Back strength). There's no difference between them(p<0.05). 4. The characteristics of the exercise stress last : The control group is higher than the experiment group in the endurance, the maximum of oxygen intake, endurance level and the out take of calory. There's little difference between them(p<0.01). 5. The characteristic of the change of the target heart rate during exercise : The control group is lower than the experiment group exchange of target heart rate, There's no difference them.

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