• 한국작물학회:학술대회논문집
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• 한국작물학회 1995년도 추계 학술대회지
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• pp.64-65
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• 1995

• 대한약침학회지
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• 제14권1호
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• pp.5-24
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• 2011

Objective: This study was performed to analyse four weeks repeated -dose toxicity of Sweet Bee Venom (SBV-pure melittin, the major component of honey bee venom) in rats. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female rats of 5 weeks old were chosen for the pilot study of four weeks repeated-dose toxicity and was injected at the level of 0.56 mg/kg body weight (eighty times higher than the clinical application dosage as the high dosage), followed by 0.28 and 0.14 mg/kg as midium and low dosage, respectively. Equal amount of normal saline was injected as the control group every day for four weeks. Results: 1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups appealed pain sense in the treating time compared to the control group, and side effects such as hyperemia and movement disorder were observed around the area of injection in all experiment groups, and the higher dosage in treatment, the higher occurrence in side effects. 3. Concerning weight measurement, neither male nor female groups showed significant changes compared to the control group. 4. Concerning to the CBC and biochemistry, all experiment groups didn't show any significant changes compared to the control group. 5. Concerning weight measurement of organs, experiment groups didn't show any significant changes compared to the control group. 6. To verify abnormalities of organs and tissues, those such as cerebellum, cerebrum, liver, lung, kidney, and spinal cords were removed and we conducted histologocal observation with H-E staining. Concerning the histologocal observation of liver tissues, some fatty changes were observed around portal vein in 0.56 mg/kg experiment group. But another organs were not detected in any abnormalities. 7. The proper high dosage of SBV for the thirteen weeks repeated test in rats may be 0.28 mg/kg in one time. Conclusion: Above findings suggest that SBV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.

• 대한한방내과학회지
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• 제26권1호
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• pp.12-47
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• 2005

First, mice were dosed with 50mg/kg of streptozotocin(STZ) twice every 24 hours to cause high blood-sugar. Then, after three days, mice were injected with 100mg/kg of STZ again. Two different dosages of Saengjihwangeumja-gami were given to the experiment groups: SA group, 15mg/kg/day, and SB group, 90mg/kg/day, in order to determine the effects of Saengjihwangeumja-gami, which has been known to be good for DM(Diabetes Mellitus). By observing weight and blood-sugar level changes, blood tolerance, the numerical value of BUN(Blood Urea Nitrogen) and creatinine in blood, and through light-electronicmicroscopic and immunohistologic investigations of pancreas and kidneys, the following results were obtained: 1. The experiment groups showed a high suppressive effect of weight-loss. 2. The experiment groups' blood-sugar and blood tolerance showed an effective lowering of blood-sugar levels. 3. The experiment groups did not show any noticeable change in the numerical value of BUN and creatinine in blood compared with that of the control groups. 4. The experiment groups showed a higher Insulin positive reaction of pancreatic islets ${/beta}-cell$ than the control groups. 5. The experiment groups showed a higher immuno-reaction against IGF- II than the control groups. 6. Observation of apoptosis of the pancreatic islets showed that the cells of experiment groups were less injured compared with those of the control groups, and fewer apoptag-positive reaction cells were seen in experiment groups than in the control groups. 7. Uunder electron-microscopy, the insulin-containing granules in pancreatic islets ${/beta}-cells$ had increased more in the experiment groups than in the control groups. 8. Under light microscopy, the injury on the inner & outer membrane of the glomerulus and epithelial cells of capillaries and cells among vessels were fewer in the experiment groups than in the control groups. 9. More apoptag-positive reaction cells in the kidney were seen in the control groups than in the experiment groups. 10. PAS-positive reaction substances had increased more in the substrate among the vessels of a glomerulus belonging to the control group than those of the experiment group. 11. Uunder electron-microscopy, the nucleonic membrane, nucleoplasm and mitochondria of proximal and distal renal tubular were more injured in the control groups than in the experiment groups. In conclusion, strong evidence for the efficacy of Saengjihwangeumja-gami in lowering blood-sugar, and in recovery and generation of pancreatic tissues injured by DM was observed. Results suggest Saengjihwangeumja-gami is an effective treatment for DM. Further study of the principles of blood-sugar dropping effects of Saengjihwangeumja-gami are needed, as well as further study of recovery and regeneration of pancreatic tissues injured by DM.

• 음성과학
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• 제14권1호
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• pp.93-103
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• 2007

The aims of this study were to examine the acoustic features of Korean and Japanese vowels, and to determine whether new phones that do not have counterparts in Japanese or similar phones that have counterparts improve more from learning. This study consisted of three parts. In Experiment I, a speech production test was performed to observe the acoustic features of Korean and Japanese vowels. In Experiment II, the speech production of Korean vowels produced by Koreans, advanced Japanese learners of Korean, and beginning Japanese learners of Korean was investigated. In Experiment III, a speech perception study of Korean vowels produced by the two Japanese learner groups was conducted to observe the effect of learning on acquiring L2 phones. The conclusion drawn from the study was that the similar phones produced by Japanese show more similarity with those of Koreans than new phones in terms of F1 and F2, but Japanese learners of Korean displayed more improvement in new phones from learning.

• 대한정형도수물리치료학회지
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• 제13권2호
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• pp.21-30
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• 2007

Purpose: The purpose of this study was to evaluate effects of lumbar vertebrae mobilization and lumbar stabilizing exercises on the muscle strength. Methods: The subject were sixty healthy adult(30 females. 30 males) two decade from 21 to 35. All subjects randomly assigned the control group, lumbar vertebrae mobilization group, lumbar stabilizing exercises group. lumbar stabilizing exercises group received lumbar stabilizing exercises for 30 minutes, lumbar vertbrae mobilization group received lumbar vertbrae mobilization for 4-5 minutes per day and three times a week during 3 weeks period. BIODEX was used to measure muscle strength. All measurements of each subjects were measured at pre-experiment, after 10 day, and post-experiment. Results: The results of this study were summarized as follows; 1. The flexion strength test of control group, lumbar vertebrae mobilization group, lumbar stabilizing exercises group were no significantly differences at pre-experiment and after 10 days(p> .05), however significantly increased post-experiment(p <.05). The result of analyzed effects of flexion strength test was significantly increased accordining to experiment type(p <.05). 2. The extension strength test of control group, lumbar vertebrae mobilization group, lumbar stabilizing exercises group were no significantly differences at pre-experiment and after 10 days(p> .05), however significantly increased post-experiment(p <.05). The result of analyzed effects of extension strength test was significantly increased accordining to experiment type(p <.05). Conclusion: conclusionally these data suggest that a 3-week lumbar stabilizing exercises improved muscle strength. Additional randomized controlled trials to more fully investigate trement effects and factors that may mediate these effect are needed.

• Plant Resources
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• 제6권1호
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• pp.16-26
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• 2003

Molecular markers are useful tools for evaluating genetic diversity and determining cultivar identity. Present study was conducted to evaluate the genetic diversity within a diverse collection of rice accessions used for Korean breeding programs. Two hundred eighty-seven rice cultivars, composed of temperate japonica, tropical japonica, indica, and Tongil-type of Korean crossing parents were evaluated by means of 15 simple sequence repeat (SSR) markers. A total of 99 alleles were detected, and the number of alleles per marker ranged from 4 to 11, with an average of 6.6 per locus. Polymorphism information content (PIC) for each of the SSR markers ranged from 0.2924 to 0.8102 with an average of 0.5785. These results, with the result that use of only 15 SSR markers made all rice cultivars examined could be uniquely distinguished, imply the efficiency of SSR markers for analysis of genetic diversity in rice. Cluster analysis was performed on similar coefficient matrics calculated from SSR markers to generate a dendogram in which two major groups corresponding to japonica (Group I) and indica and Tongil type rice (group II) with additional subclasses within both major groups. The narrowness of the Korean breeding germplasm was revealed by the fact that most of the Korean-bred and Japan-bred temperate japonica cultivars were concentrated into only 2 of the sub-group I-1 (143 cultivars) and I-2 (58 cultivars) among six sub-groups in major group of japonica. This is because of the japonica accessions used in this study was a very closely related ones because of frequent sharing of the crossing parents with similar genetic background with synergy effect of the inherited genetic difference between indica and japonica. A rice breeding strategy with the use of molecular markers was discussed for overcoming of genetic vulnerability owing to this genetic narrowness.

• The Journal of Korean Physical Therapy
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• 제16권3호
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• pp.9-21
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• 2004

The purpose of study is that we will observe the change of c-fos with the immunohistochemistry method and then we will study the effect of micro current stimulation following the frequency after inducing pain to rats with capsaicin. Rat were classified to SD and they have growed for 8 weeks. We classify to four groups, ordinal group is used in experiment I, the group which we induce pain is used in experiment II, the application group which we induce pain and then the high frequency micro current stimulation is used in experiment III, the application group which we induce pain and then the low frequency micro current stimulation is used in experiment IV, we get the following result. Compare with experiment II, experiment III, and experiment IV from acute pain modal in the immunohistochemistry experiment which has c-fos protein as an antigen, c-fos immunoreactive positive neurons significantly after induced pain for two hours(p<0.001). According to these results, from rats induced pain, micro current stimulation effect to reducing pain, but following frequency micro current stimulation theraphy isn't different from immunoreactive c-fos

• 한국작물학회지
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• 제48권1호
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• pp.56-64
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• 2003

If a quantitative trait loci (QTL) marker identified in a population is applicable to different populations (marker universality), this will not only reduce the labor and cost in marker assisted selection (MAS), but accelerate the application of molecular markers to real breeding programs. Present study aims to evaluate the defined QTL related markers from a population to a different breeding population for the MAS. Four rice breeding populations were subjected to seventy-five simple sequence repeat (SSR) markers which were already identified for their polymorphism information content (PIC) in the parents of the crossings. Among them, eight markers were evaluated for their correlation between presence of marker alleles and phenotypic expression in breeding populations. A reasonable level of polymorphism for the mapped markers originated from any sources of rice accessions was observed between crosses of any sources (marker repeatability). However, correlation between presence of markers and expression of the traits in rice breeding populations was not significant except for minor portion of traits and markers examined (failure of marker universality). In the present study, various strategies were discussed to develop new markers with universality of breeding application.

• 한국과학교육학회지
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• 제19권1호
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• pp.78-90
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• 1999

본 연구에서는 실험에 포함된 이상조건의 이해를 조사하기 위해 수행되었다. 주요 연구질문은 다음과 같다. (1) 실제 실험 상황에는 어떠한 이상조건이 포함 되어 있다고 생각하는가? (2) 실험에 포함된 이상조건들이 실제 실험에서 얼마나 만족되고 있다고 생각하는가 ? (3) 실험에 포함된 이상조건들이 실험 결과에 얼 마나 영향을 준다고 생각하는가 ? (4) 교사들은 실험에 포함된 이상조건에 대해 일반적으로 어떠한 관점을 가지고 있는가 ? 연구질문 1, 2, 3을 위해서 6개의 설문을 개발하여 총 85명의 고등학생과 과학교사에게 직접 실험과 함께 제시하여 응답하도록 하였으며, 연구질문 4를 위해서 과학교사 42명을 대상으로 4개의 설문을 개발하여 응답하도록 하였다. 응답결과는 연구 질문에 따라 분류되어 요약 제시되었으며, 물리 실험 지도를 위한 시사점 추출을 위해 사용되었다.

• 말소리와 음성과학
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• 제2권2호
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• pp.101-108
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• 2010

An inhaled salbutamol and salmeterol for chronic obstructive pulmonary disease(COPD) and asthma have been used worldwidely. But there has been few study about the voice change evoked from the post-medicine effect. To evaluate the voice influenced of short-acting and long-acting ${\beta}_2$-agonists, two experiments were carried out: one was salbutamol experiment 1 with eight patients, the other was salmeterol experiment 2 with six patients. Experiment 1 was made of two stages: premedication & postmedication. Experiment 2 was four stages: stageI was premedication, stageII was postmedication & pregaggling, stageIII was postmedication & postgaggling(100 ml with water), and stageIV was postmedication & 30 minutes later. Measured parameters were F0, F0_SD, Jitter_rap, Shimmer_apq11, HNR, BW(1, 2, 3), Intensity, and H1-H2. The mean data collected from 3 repetitions each was statistically analyzed by Wilcoxon signed rank test for experiment 1 and repeated measures ANOVA for experiment 2. In experiment 1, significant differences were found in the Jitter_rap(Z= -2.10, p=0.036). The findings indicated that the postmedicated voice was worse than premedicated voice. In experiment 2, there wasn't significant difference, but values of parameters related to voice quality(Jitter_rap, Shimmer_apq11, HNR, and H1-H2) showed changes toward stageⅣ, that is, the voice quality was worse under medication.