• 제목/요약/키워드: Exotic Plant

검색결과 137건 처리시간 0.028초

Identification of a Third Haplotype of the Sequence Linked to the Restorer-of-fertility (Rf) Gene and Its Implications for Male-Sterility Phenotypes in Peppers (Capsicum annuum L.)

  • Min, Woong-ki;Lim, Heerae;Lee, Young-Pyo;Sung, Soon-Kee;Kim, Byung-Dong;Kim, Sunggil
    • Molecules and Cells
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    • 제25권1호
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    • pp.20-29
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    • 2008
  • Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

Evaluation and Verification of Barley Genotypes with Known Genes for Resistance to Barley yellow mosaic virus and Barley mild mosaic virus Under Field Conditions in South Korea

  • Kim, Hong-Sik;Baek, Seong-Bum;Kim, Dea-Wook;Hwang, Jong-Jin;Kim, Si-Ju
    • The Plant Pathology Journal
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    • 제27권4호
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    • pp.324-332
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    • 2011
  • Soil-borne barley yellow mosaic disease caused by Barley yellow mosaic virus (BaYMV) or Barley mild mosaic virus (BaMMV) gives a serious threat to the winter barley cultivated in the southern regions in Korea. It is important to develop resistant varieties for stable and high-yield production. The objectives of this study were to evaluate 22 genotypes of exotic barley germplasms carrying the resistance genes rym1 through rym12, with the exception of rym10, and to determine the genes that confer resistance to BaYMV or BaMMV in Korea. Using the traditional visual scoring of symptoms at 4 locations over 3 years, average disease rate values differed (P < 0.001) among the genotypes. ELISA test revealed the presence of both BaYMV and BaMMV in all of the field sites but Jinju and significantly different rates of infection among genotypes and years. Barley genotypes differed in how virus quantities and pathogen-induced symptoms were correlated, especially in response to BaYMV. Disease incidence was affected by the climatic conditions present during the early growing stage before overwintering. A Chinese landrace, 'Mokusekko 3', carrying rym1 and rym5 was comparatively resistant at all locations studied. The barley genotypes carrying either rym6 or rym9 were susceptible to the viral strains. The genotypes carrying rym5 were resistant in Jinju and Milyang but susceptible in Iksan and Naju. The resistance genes rym2 and rym3 were effective in local strains and would be potent contributors to disease resistance.

소면적 재배작물인 당귀밭에 발생하는 잡초현황과 잡초에 의한 피해 (Occurrence of Weed Flora and Their Yield Loss in Angelica gigas Upland Fields of Minor Crop in Korea)

  • 이인용;김창석;이정란;서영진;김종수;서현아;장형목
    • Weed & Turfgrass Science
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    • 제5권2호
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    • pp.65-70
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    • 2016
  • 충북 제천, 경북 봉화를 중심으로 당귀밭에 발생하는 잡초를 조사하여 당귀밭 문제잡초의 현황을 파악하고 잡초방제의 기초자료로 활용하기 위하여 2015년 5월과 6월에 실시하였다. 당귀밭에 발생하는 잡초는 37과 105초종으로 국화과 20종, 화본과 9종, 두과, 마디풀과, 사초과 각각 6종, 꿀풀과, 메꽃과 각각 5종 등의 순이었다. 이들 잡초를 생활형으로 구분하면, 일년생 53종, 월년생 24종, 다년생 28종이었다. 우점잡초로는 바랭이, 쇠비름, 흰명아주, 참방동사니, 망초, 털별꽃아재비 등이었다. 외래잡초는 27종으로 25.7%를 점유하고 있으며, 흰명아주, 망초, 털별꽃아재비, 개비름, 서양민들레, 개망초 순으로 우점도가 높았다. 잡초에 의해 당귀 수량은 49.6% 감소되었다.

병솔꽃나무(Callistemon citrinus (Curt.) Skeels)의 개화결실 및 증식특성 (Characteristics of flowering, fruit setting and propagation of Callistemon citrinus (Curt.) Skeels)

  • 김찬수;정은주;김지은;소인섭
    • 한국자원식물학회지
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    • 제15권2호
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    • pp.107-113
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    • 2002
  • 본 연구는 외래도입 병솔꽃나무(Callistemon citrinus)의 개화결실 습성과 효율적인 증식방법을 구명 하고자 수행하였다. 병솔꽃나무의 삭과는 개화후 4년간 가지에 존속하며, 종자의 수와 크기는 3년생 삭과와 2년생 삭과가 비슷한 반면 1년생 삭과는 크게 차이가 나는 것으로 나타났다. 삭과로부터 종자를 탈리시키기 위해 건조시킨 결과 삭과의 상대 수분함량이 약 75%일 때 삭과열개율이 100%에 달했다. 발아율은 3$0^{\circ}C$에서 건조시킨 종자가 가장 높았으며, 그 중 2년생 삭과에서 탈리된 종자의 발아율이 높았다. 반면 50, 7$0^{\circ}C$에서 건조되어 탈리된 종자의 발아율은 낮았다. 가을철 2차 개화를 유도하기 위하여 봄철 개화 후 삭과가 달린 가지를 전정한 개체에서는 모든 개체에서 가을에 개화하였으며 , 개화량은 평균 14송이였다. 반숙지를 IBA 100mgι$^{-1}$ 에 24시간 침적처리한 결과 86.4%가 발근되었으며, 평균 12.6개의 1차근을 가지며 생장이 가장 양호하였다.

Development of Neutaribeosut Varieties (Pleurotus ostreatus) Chongpung, Myongwol

  • Lee, He-Duck;Kim, Hong-Kyu;Kim, Young-Gyun;Han, Kyu-Heng;Min, Kwang-Ho;Jeong, Tae-Wo;Lee, Jae-Jung;Jeon, Tae-Sun;Kim, Duck-wan;Kim, Hun-Sick;Han, Jong-Ku
    • Plant Resources
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    • 제3권2호
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    • pp.105-109
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    • 2000
  • This study was carried out to find a useful mushroom at Chungnam Agricultural Research And Extention Service. Twenty materials used were collected from domestic and exotic area. These races were compared bontanical characteristics to leading varieties by PCR-RAPD methods. Mycelial growth temperature of Chongpung and Myongwol were at 20 to 25 $^{\circ}C$ and 25 to 30 $^{\circ}C$ at PDA medium, respectively mycelial growth of these varieties were similiar at pH 6.5 to 7.5. In case of mushroom cultivation temperature ranges, Chongpung was at 5 to 26$^{\circ}C$ and Myongwol was at 7 to 28$^{\circ}C$, but the optimum temperature range of these were appeared at 15 to 19$^{\circ}C$. Culture temperature of these was 23$^{\circ}C$ and period of mycelial culture was needed 23 to 24 days under 850cc/pp, while was needed 11 to 12 days at waste cotton medium. Cap color of these at first inducing mushroom was all dark blue, but at late growing stages Chongpung was shown as grey, and Myongwol was shown as dark grey. Yield of Chongpung was appeared as 46kg/3.3$m^2$ and that of Myongwol was 41kg /3.3$m^2$, while Chunchu No2 as check was 40kg/3.3$m^2$. Results from PDA medium and PCR-RAPD analysis two of these were different from others.

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Transferability of EST SSR-Markers from Foxtail Millet to Barnyard Millet (Echinochloa esculenta)

  • Myung Chul Lee;Yu-Mi Choi;Myoung-Jae Shin;Hyemyeong Yoon;Seong-Hoon Kim
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 춘계학술대회
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    • pp.45-45
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    • 2020
  • A large number of expressed sequence tags (ESTs) in public databases have provided an opportunity for the systematic development of simple sequence repeat (SSR) markers. EST-SSRs derived from conserved coding sequences show considerable cross-species transferability in related species. In the present study, we assessed the utility of foxtail millet EST-SSRs in barnyard millet. A total of 312 EST-SSRs of foxtail millet were tested using 84 Echinochloa crus-galli germplasm accessions; a high rate of transferability (62%) and 46 primer sets (13%) were shown the polymorphism in barnyard millet. The 13% of functional EST-SSRs) was demonstrated between cereals and barnyard millet. SSR marker profile data were scored for the computation of pairwise distances as well as a Neighbor Joining (NJ) tree of all the genotypes. The averaged values of gene diversity (HE) and polymorphism information content (PIC) were 0.213 and 0.179 within populations, respectively. The 84 barnyard millet germplasm accessions were divided into five different groups, which agreed well with their geographical origins. The exotic 12 accessions of India type barnyard millet (E. frumentacea) were all separated form Korean local collection genotype. The present results provide evidence of divergence between cultured and wild type barnyard, as a millet and grass. The polymorphic SSR markers indicated in this study were of great value in analysis of genetic diversity that can be further used for crop improvement through breeding.

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경관훼손지의 생태적 복구방안에 관한 연구 (A Study on the Ecological Restoration Strategies for the Disturbed Landscapes)

  • 김남춘
    • 한국환경복원기술학회지
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    • 제1권1호
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    • pp.28-44
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    • 1998
  • This study was conducted to suggest the ecological restoration strategies for the disturbed landscapes by theoretical study. Especially, it is aimed to suggest three objectives for restoration by using native plants ; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Cooperation between restoration ecologists and restoration practitioners is needed to delineate a scientific approach to restoration ; (1) Being aware of published literature that describes similar work and/or establish general principles. (2) Preparedness to carry out proper experiments to test ideas. (3) Preparedness to monitor fundamental parameters in a restoration scheme. (4) Providing information about the behavior of species. (5) Publish results. 2. There are three models of succession in theories of plant succession. The tolerance and facilitation models were recommended to ecological restoration. The inhibition model applies in most secondary succession, but the actual species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. 3. The objectives of erosion control, wildlife habitat provision, and visual quality improvement are not mutually exclusive. However, many revegetation practices in the past have emphasized one of these aspects at the expense of the others. 4. A native plant community can be the model of ecological restoration. By stylization/abstraction of native plant community, trying to learn the most essential characteristics of community types - environmental factors ; dominant, prevalent, and "visual essence" species composition - in order to use such information in restoration. 5. After developing mass/spaces plan, match plant communities to the mass/space plan. In utilizing community grouping, there needs aesthetic ability to understand design elements. 6. Several hydrophytes such as Pennisetum saccharifluous, Themeda triandra, Cirsium pendulum show relatively good germination rates. In case of mesophytes and xerophytes, Arundinella hina, Artemisia princeps, Oenothera odorata and legumes seem to have quick-germinating abilities at barren sites. Pinus thunbergii, Rhus chinensis, Evodia daniellii, Alnus firma and Albizzia julibrissin can be considered as "late succession" woody plants because they show low germinating rates and slow growing habitat. 7. The seeds used for restoration should be collected within a certain radius of where it will be planted. Consideration in genetic issues in the collection and use of germplasm can increase the odds for successful restoration efforts. 8. The useful model in the "drift" pattern occurs so abundantly in naturally evolving landscapes. As one species diminishes in density, a second or third species are increased. Thus, dynamic interactions between species are created. Careful using of "drift phenomenon" in planting was recommended. 9. Virtually no stand of vegetation today is immune from the introduction and/or spreading of exotic species. Therefore, the perpetuation of a restored sites requires conscious monitoring and management. Thus, management would be the most important process in ecological restoration. 10. In order to keep the sites "natural", alternative management strategies would be applied in management ; Atenative management strategies are decelerating successional process, accelerating successional processes or modifying species composition. As management tools, mechanical, chemical, biological or environmental factor manipulation, e.g., fire could be used.

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검역잡초종자 발아력 제거를 위한 수입농산물 열처리 효과 (Heat-treatment Effects of Agricultural Imports for Devitalization of Quarantine Weed Seeds)

  • 문광옥;오진보;경은선;이용호;홍선희;강병화
    • Weed & Turfgrass Science
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    • 제2권2호
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    • pp.170-175
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    • 2013
  • 우리나라는 열처리방법을 포함한 식물검역규정에 의하여 외래잡초의 유입방지를 위한 잡초검역을 실시하고 있다. 현행 열처리기준의 적절성을 확인하고 검역잡초의 발아력을 완전히 제거하기 위한 열처리조건을 구명하여 식물검역에 활용하기 위하여 검역잡초 9종과 검역잡초와 유사한 잡초 1종의 종자를 재료로 하여 건열처리와 상대습도 40%의 습열처리에 의한 사멸조건을 연구하였다. 다양한 온도 및 시간별 건열처리 결과 $95^{\circ}C$에서 48시간, $100^{\circ}C$에서 36시간, $110^{\circ}C$에서 24시간, $121^{\circ}C$에서 4시간, $130^{\circ}C$에서 30분 처리 후 모든 종자가 사멸되었고, 상대습도 40%에서의 습열처리 결과 $85^{\circ}C$에서 36시간, $90^{\circ}C$에서 16시간 습열처리 후 모든 종자가 사멸하였다. 상기의 결과는 현행 열처리기준이 검역잡초 몇 종에는 적용하기에 적합하지 않음을 보여주며, 검역잡초종자의 발아력을 제거할 수 있는 이 열처리조건은 수입농산물을 통한 검역잡초의 유입을 방지하기 위한 검역의 한 방법으로 적용할 수 있을 것이다. Picris echioides와 같이 강한 내열성이 있는 종과 새삼속(Cuscuta spp.)과 같이 열에 민감한 종을 위한 별도의 열처리조건이 필요하며 이는 다른 내열성의 검역잡초 종자를 사멸시키는 검역업무의 효율성을 향상시킬 수 있을 것으로 사료된다.

외래잡초 미국좀부처꽃(Ammannia coccinea)의 확산과 생육지 특성 (Spreading and Distribution of Exotic Weed Ammannia coccinea in Korea)

  • 황선민;길지현;김영하;김성열
    • Weed & Turfgrass Science
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    • 제3권4호
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    • pp.292-298
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    • 2014
  • 본 연구는 부처꽃과에 속하는 북아메리카 원산의 외래잡초이며 농경지에서벼와 경합을 하는 것으로 알려져 있는 미국좀부처꽃(Ammannia coccinea)의 분포와 생육지 유형별 특성을 조사하여, 관리 계획에 필요한 기초적인 자료를 제공하고자 하였다. 미국좀부처꽃은 자연상태의 생육지에서 넓은 면적으로 우점 분포하는 것은 확인되지 않았지만, 전라남도, 경상도, 충청도, 강원도 등 전국적으로 흩어져 분포하고 있다. 생육지 유형은 크게 논 주변부, 묵논, 하천습지, 저수지 등 4가지로 구분되었다. 조사된 지점들 중 많은 지점이 농경지 주변의 습지 지역으로 벼농사를 위해 농수로가 연결되어 있는 환경이었다. 다수의 조사지점에서 농수로가 흐르는 방향을 따라 미국좀부처꽃이 분포하는 것으로 볼 때 미국좀부처꽃은 물길을 따라 확산되는 것으로 판단된다. 미국좀부처꽃의 생육지에서 조사된 관속식물은 총 27과 51속 64종 5변종 등 모두 69분류군이 생육하는 것으로 조사 되었다. 생활형을 분석한 결과 일년생식물(Th)이 36분류군, 52%로 가장 높게 출현하여 미국좀부처꽃의 생육지가 교란이 많이 일어나는 지역임을 확인할 수 있었다.

녹화(綠化)와(と) 인간(人間)(III) - 비탈면 녹화(綠化)의 전망(展望) - (Revegetaton and human(III) - Perspective on the revegetation of the face of slopes -)

  • 전근우;서문원;서정일;안영상;김인애;이노우에 슈지;이와모토 토루;에자키 츠지오
    • Journal of Forest and Environmental Science
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    • 제18권1호
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    • pp.15-30
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    • 2001
  • 21 세기에 들어 재래목본식물(在來木本植物)을 이용한 비탈면 녹화(綠化)가 이용되기 시작되고 있으므로 그 발전방향을 예측하기 위해 녹화(綠化)의 기본적인 개념과 녹화(綠化)에 대한 주역주민의 인식을 정리하여 보았다. 조사결과, 지역주민은 녹화(綠化)의 필요성과 재래목본식물(在來木本植物)을 이용한 공법(工法)을 충분히 인식하고 있었으나, 외래종(外來種)의 도입에 대해서는 대부분의 주민이 부정적이었다. 따라서 앞으로는 비탈면 녹화(綠化)의 다면성(多面性)을 추구해야 하며, 재래목본식물(在來木本植物)의 도입이 곤란한 장소에는 재래잡초류(在來雜草類)의 이용을 추진해야 할 것이다. 또한 비탈면에 시공하고 있는 금망(金網)은 인위적(人爲的) 재해(災害)를 유인할 가능성이 있으므로 시정할 필요가 있다. 결론적으로 비탈면 녹화(綠化)는 단기적으로는 비탈면의 물리적(物理的) 안정(安定)과 생태적(生態的) 안정(安定)에, 중 장기적으로는 지구환경(地球環境)의 개선(改善)과 향상(向上)에 기여해야 할 것이다.

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