• Proceedings of the Botanical Society of Korea Conference
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• 1987.07a
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• pp.133-148
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• 1987

Gibberellin(GA) 3-$\beta$ hydroxylation is very important for the shoot elogation in the higher plants, since only 3$\beta$-hydryoxylated GAs promote shoot elogation in several plants. Fluctuation of 3$\beta$-hydryoxylase activity was examined during seed maturation using two cultivars of , P. vulgaris, Kentucky Wonder (normal) and Masterpiece (dwarf). Very immature seeds of both cultivars contain high level of 3$\beta$-hydroxylase activity (per mg protein). Both cultivars showed maximum of enzyme activity (per seed) in the middle of their maturation process. Gibberellin 3$\beta$-hydroxylase catalyzing the hydroxylation of GA20 to GA1 was purified 313-fold from very early immature seeds of P. vulgaris. Crude soluble enzyme extracts were purified by 15% methanol precipitation, hydrophobic interaction chromatogrphy, DEAE ion exchange column chromatography and gel filtration HPLC. The 3$\beta$-hydroxylase activity was unstable and lost much of its activity duting the purification. The molecular weight of purified enzyme was extimated to be 42, 000 by gel filtration HPLC and SDS-PAGE. The enzyme exhibited maximum activity at pH 7.7. The Km values for [2.3-3H] GA20 and [2.3-3H]GA9 were 0.29 $\mu$M and 0.33 $\mu$M, respectively. The enzyme requires 2-oxoglutarate as a cosubstrate; the Km value for 2-oxoglutarate was 250 $\mu$M using 3H GA20 as a substrate. Fe2+ and ascorbate significantly activated the enzyme at all purification steps, while catalase and BSA activated the purified enzyme only. The enzyme was inhibited by divalent cations Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+. Effects of several GAs and GA anaogues on the putrified 3$\beta$-hydroxylase were examined using [3H]GA9 and GA20 as a substrates. Among them, GA5, GA9, GA15, GA20 and GA44 inhibited the enzyme activity. [13C, 3H] GA20 was converted by the partially purified enzyme preparation to [13C, 3H]GA1, GA5 and GA6, which were identified by GC-MS, GA9 was converted only GA4, GA15 and GA44 were converted to GA37 and GA38, respectively. GA5 was epoxidized to GA6 by the preparation. This suggests that 3$\beta$-hydroxylation of GA20 and epoxidation of GA5 are catalyzed by the same enzyme in P, vulgaris.

• Journal of the Korean Magnetics Society
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• v.21 no.4
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• pp.127-131
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• 2011

In this paper, we study the electronic and magnetic properties of Cr substituted SiTe in the rock-salt structure compound using the full potential linearized augmented plane wave method within the generalized gradient approximation to the exchange correlation potential. Two stoichiometries are studied: $CrSi_3Te_4$ with 25 %, and $CrSiTe_2$ with 50 % Cr substitution. We found, from the total energy calculations, that the equilibrium lattice constant for cubic $CrSi_3Te_4$ is 11.64 a.u. and a = 7.89 a.u. and c = 11.13 a.u. for tetragonal $CrSiTe_2$. The integer value of the calculated magnetic moment per unit cell, $4{\mu}_B$ for $CrSiTe_2$ suggests that this compound is halfmetallic. The magnetic moment per unit cell for $CrSi_3Te_4$ is slightly larger than $4{\mu}_B$. The magnetic moment on Cr atoms are 3.61 and $3.62{\mu}_B$ in the $CrSi_3Te_4$ and $CrSiTe_2$, respectively. The presence of Cr atoms causes that the other atoms become slightly magnetized in both compounds. The electronic properties and the magnetism are discussed with the calculated spin-polarized density of states.

• Journal of the Korean Magnetics Society
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• v.9 no.6
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• pp.271-277
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• 1999

Amorphous $Fe_{83}B_9Nb_7Cu_1$ alloy has been studied by M$\"{o}$ssbauer spectroscopy. Revised Vincze method was used and distributions of hyperfine field, isomer shift, and quadrupole line broadening of the sample at various temperatures have been evaluated and Curie temperature and $H_{hf}\;(0)$ were calculated to be 393 K and 231 kOe, respectively. Temperature variation of reduced average hyperfine field shows a flattered curvein comparison with the Brillouin curve for S=1. This behavior can be explained on the basis of Handrich molecular field model, in which the parameter Δ, which is a measure of fluctuation in exchange interactions, is assumed to have the temperature dependence ${Delta}=0.75-0.64{\tau}+0.47{\tau}^2$ where $\tau$ is $T/T_C$. At low temperature, the average hyperfine field can be fitted to $H_{hf}\;(T)=H_{hf}\;(0)\;[1-0.44\;(T/T_C)^{3/2}-0.28(T/T_C)^{5/2}-… ]$, which indicates the presence long wave length spin wave excitations. At temperature near TC, reduced average hyperfine field varies as $1.00\;[1-T/T_C]^{0.39}$. It is also found that half-width of the hyperfine field distribution was 102 kOe (3.29 mm/s) at 13 K and decreased monotonically as temperature increased. Above the Curie temperature, an average quadrupole splitting value of 0.43 mm/s was found. Average line broadening due to quadrupole splitting distribution was 0.31 mm/s at 13 K and decreases monotonically to 0.23 mm/s at 320 K, whereas that due to the isomer shift distribution is 0.1 mm/s at 13 K and 0.072 mm/s at 320 K, which is much smaller than that of both hyperfine field and quadrupole splitting. The temperature dependence of the isomer shift can be fitted within the harmonic approximation to a Deybe model with a Debye temperature ${Theta}_D=424{\pm}5K$.TEX>.

• Korean Journal of Food Science and Technology
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• v.35 no.4
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• pp.628-634
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• 2003

To investigate the effects of storage and pasteurization temperature on the quality of kiwi juice $(13.5^{\circ}Brix)$, the Kiwi juice was pasteurized at 65, 75 and $85^{\circ}C$ for 15 sec. The microbial, physicochemical measurements and sensory evaluations were conducted at the same condition during storage at 4 and $25^{\circ}C$ for 30 days. Most of the vegetative bacteria cells in kiwi juice were destroyed by heat treatment over $65^{\circ}C$, and they did not actively grow in kiwi juice after pasteurization. The D values of bacteria in kiwi juice by tubular type of heating exchange were 4.17, 1.47 and 0.81 sec at 65, 75 and $85^{\circ}C$, respectively. The growth of microorganisms during storage were not detected in the most samples. The amounts of vitamin C decreased as the pasteurization-storage-temperature and storage time increased. While reducing sugar increased as the pasteurization-storage temperature-storage time increased, it decreased rapidly after 20 days of storage. Hunter's color values L, a and b of stored kiwi juice were decreased at all storage conditions, browning reaction rate increased as the pasteurization-storage-temperature was decreased.

• The Korean Journal of Financial Management
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• v.27 no.1
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• pp.89-120
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• 2010

In this paper, we analyse empirically the relations between ownership structure and cash holdings of firms listed on Korea Securities Market and Kosdaq Market of Korea Exchange. The main results of this study can be summarized as follows. Cash holdings increase as large shareholder's equity holdings increase. Cash holdings increase as the difference between first largest shareholder's and second largest shareholder's equity holdings increase, and cash holdings increase as the ownership concentration increase. Managerial ownership exert a non-linear effects on cash holdings. So to speak, at lower level of managerial ownership, managers hold more cash to pursue their own interests at the expense of minority shareholders, but at higher level of managerial ownership, the interests of managers and shareholders are aligned, and also at highest level of managerial ownership, managers hold more cash to pursue their own interests at the expense of minority shareholders. Cash holdings increase larger in owner-controlled firm than in management-controlled firm. These results support the expropriation of minority shareholders hypothesis that large shareholders can extract private benefits from corporate resources under their control at the expense of minority shareholders. This paper contributes to defining information value of large shareholder's equity holdings on cash holdings for a firms' other stakeholders such as investors and creditors, and to strengthening a legal and institutional safeguard for external minority shareholders. Ownership concentration might have negatively affected the evolution of the legal and institutional frameworks for corporate governance and the manner in which economic activity is conducted. It could be a formidable barrier to future policy reform.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.5
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• pp.1087-1096
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• 2011

Vehicular Ad Hoc Network(VANET) using wireless network is offering the communications between vehicle and vehicle(V2V) or vehicle and infrastructure(V2I). VANET is being actively researched from industry field and university because of the rapid developments of the industry and vehicular automation. Information, collected from VANET, of velocity, acceleration, condition of road and environments provides various services related with safe drive to the drivers, so security over network is the inevitable factor. For the secure message authentication, a number of authentication proposals have been proposed. Among of them, a scheme, proposed by Jung, applying database search algorithm, Bloom filter, to RAISE scheme, is efficient authentication algorithm in a dense space. However, k-anonymity used for obtaining the accurate vehicular identification in the paper has a weak point. Whenever requesting the righteous identification, all hash value of messages are calculated. For this reason, as the number of car increases, a amount of hash operation increases exponentially. Moreover the paper does not provide a complete key exchange algorithm while the hand-over operation. In this paper, we use a Received Signal Strength Indicator(RSSI) based velocity and distance estimation algorithm to localize the identification and provide the secure and efficient algorithm in which the problem of hand-over algorithm is corrected.

• Journal of KIISE:Information Networking
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• v.29 no.1
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• pp.65-76
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• 2002

QoS routing is a routing technique for finding feasible path that satisfies QoS requirements required by application programs. Since QoS routing determines such paths in terms of dynamic network state, it satisfies the requirement of applications and increases the utilization of the network. The overhead is, however, generated by routers to exchange the information of the dynamic state of network. In order to reduce this protocol overhead, a timer based update mechanism is proposed in which router checks the change of the network status periodically and network state information is exchanged if the change is greater than a certain value. Using large update period makes, though, routing performance irresponsive to the parameters which determine the update of the network state of the router. In addition to this, large update period may result in inaccurate network state information at routers and cause resource reservation failure. The resource reservation failure generates additional overhead to cancel the resource reservation of the part of the path. In this paper, we propose mechanisms enhancing the existing network state update policy with respect to these two problems. Performance of the proposed schemes are evaluated through a course of simulation.

• Journal of Astronomy and Space Sciences
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• v.26 no.2
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• pp.237-266
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• 2009

In 1631 A.D. Jeong Duwon, an ambassador of the Joseon dynasty was sent to the Ming dynasty. There he met $Jo\tilde{a}o$ Rodrigues, a Jesuit missionary, in Dengzhou of Shandong peninsula. The missionary gave the ambassador a number of results of latest European innovations. A detailed description on this event was written in 'Jeong's official report regarding a message from an European country'(西洋國奇別狀啓), which is an important literature work to understand the event. Since the document was written in classical Chinese, we make a comprehensive translation to Korean with detailed notes. According to the report, the items that Rodrigues presented include four books written in Chinese that describe European discoveries about the world, a report on the tribute of new cannons manufactured by Portuguese in Macao, a telescope, a flintlock, a Foliot-type mechanical clock, a world atlas drawn by Matteo Ricci, an astronomical planisphere, and a sun-dial. We discuss the meaning of each item in the Korean history of science and technology. In particular, Jeong's introduction is an important event in the history of Korean astronomy, because the telescope he brought was the first one to be introduced in Korean history. Even though king Injo and his associates of the Joseon dynasty were well aware of the value as military armaments of new technologies such as telescopes, cannons, and flintlocks, they were not able to quickly adopt such technologies to defend against the military threat of Jurchen. We revisit the reason in view of the general history of science and technology of east-Asian countries in the 17th century.

• Korean Journal of Poultry Science
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• v.17 no.2
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• pp.109-114
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• 1990

Enzymatic activity of isolated Lysozyme from egg white by cation ion-exchange chromatography was detected with various methods and stability of lysozyme in solution was studied by heat and pH treatments. Lysozyme activity refered to mg pure lysozyme/mg sample was more accurate although it needed standard lysozyme. But lysozyme activity refered to units/mg sample could be detected easily and reducted total detection time. Enzymatic activity of isolated lysozyme which dissolved in 0.066M phosphate buffer(pH 6.3) and then incubated at $37^{\circ}C$ for 2hr was increased remarkably on the lysis of Micrococcus lysodeikticus. The activity of isolated lysozyme by CM Sephadex C-25 was higher in eluting solution of above O. D. 1.0 at 640nm and attained 36, 000 units/mg solid. The stability of isolated lysozyme was decreased by various heat treatment. Activity began to decrease above 6$0^{\circ}C$ and dropped rapidly at $100^{\circ}C$. Especially, 35% loss of activity occured in 0.066M phosphate buffer at $100^{\circ}C$. for 15min. The stability of lysozyme was also affected by pH. lysozyme was very stable in acidic solution but in alkaline solution. Enzymatic activity showed maximum value at pH 3.0 solution while decreased rapidly above pH 6.0 solution.

• Journal of Internet Computing and Services
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• v.17 no.6
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• pp.33-39
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• 2016

With recent advances in sensor technology, CMOS-based semiconductor devices and networking protocol, the areas for application of wireless sensor networks greatly expanded and diversified. Such diversification of uses for wireless sensor networks creates a multitude of beneficial possibilities for several industries. In the application of wireless sensor networks for monitoring systems' data transmission process from the sensor node to the sink node, transmission through multi-hop paths have been used. Also mobile sink techniques have been applied. However, high energy costs, unbalanced energy consumption of nodes and time gaps between the measured data values and the actual value have created a need for advancement. Therefore, this thesis proposes a new model which alleviates these problems. To reduce the communication costs due to frequent data exchange, a State Prediction Model has been developed to predict the situation of the peripheral node using a geographic autocorrelation of sensor nodes constituting the wireless sensor networks. Also, a Risk Analysis Model has developed to quickly alert the monitoring system of any fatal abnormalities when they occur. Simulation results have shown, in the case of applying the State Prediction Model, errors were smaller than otherwise. When the Risk Analysis Model is applied, the data transfer latency was reduced. The results of this study are expected to be utilized in any efficient communication method for wireless sensor network monitoring systems where all nodes are able to identify their geographic location.