• 원자력산업
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• 제6권5호통권39호
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• pp.8-11
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• 1986

• 대학교육
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• 통권8호
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• pp.81-86
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• 1984

• 세라미스트
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• 제6권2호
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• pp.41-47
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• 2003

• Bulletin of the Korean Chemical Society
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• 제27권10호
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• pp.1581-1586
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• 2006

A La (III) ion-selective membrane sensor has been fabricated from poly vinyl chloride (PVC) matrix membrane, containing 3-hydroxy-N'-(pyridin-2-ylmethylene)-2-naphthohydrazide (HPMN) as a neutral carrier, potassium tetrakis (p-chlorophenyl) borate (KTpClPB) as an anionic excluder and ortho-nitrophenyloctyl ether (NPOE) as a plasticizing solvent mediator. The effects of membrane composition and pH as well as the influence of the anionic additive on the response properties were investigated. The sensor with 30% PVC, 62% solvent mediator, 6% ionophore and 2% anionic additive, shows the best potentiometric response characteristics. It displays a Nernstian behavior (19.2 mV per decade) across the range of $1.0{\times}10^{-2}-1.0{\times}10^{-7}$ M. The detection limit of the electrode is $7.0{\times}10^{-8}$ M ($\sim$10 ng/mL) and the response time is 15 s from $1.0{\times}10^{-2}$ up to $1.0{\times}10^{-4} $M and 30 s in the range of $1.0 {\times}10^{-5}-1.0{\times}10^{-7}$ M. The sensor can be used in the pH values of 3.0-9.0 for about seven weeks. The membrane sensor was used as an indicator electrode in the potentiometric titration of lanthanum ions with EDTA. It was successfully applied to the lanthanum determination in some mouth wash preparations.

• BMB Reports
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• 제40권4호
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• pp.501-510
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• 2007

Sex-related genes expressed in vitellogenic ovaries of the giant tiger shrimp, Penaeus monodon, were identified by an EST approach. A total of 1051 clones were unidirectionally sequenced from the 5 terminus. Nucleotide sequences of 743 EST (70.7%) significantly matched known genes previously deposited in the GenBank (E-value <$10^{-4}$) whereas 308 ESTs (29.3%) were regarded as newly unidentified transcripts (E-value >$10^{-4}$). A total of 559 transcripts (87 contigs and 472 singletons) were obtained. Thrombospondin (TSP) and peritrophin (79 and 87 clones accounting for 7.5 and 8.3% of clones sequenced, respectively) predominated among characterized transcripts. everal full length transcripts (e.g. cyclophilin, profillin and thioredoxin peroxidase) were also isolated. A gene homologue encoding chromobox protein (PMCBX, ORF of 567 nucleotides encoding a protein of 188 amino acids) which is recognized as a new member of the HP1 family was identified. Expression patterns of 14 of 25 sex-related gene homologues in ovaries and testes of P. monodon broodstock were examined by RT-PCR. Female sterile and ovarian lipoprotein receptor homologues were only expressed in ovaries whereas the remaining transcripts except disulfide isomerase related P5 precursor and adenine nucleotide translocator 2 were higher expressed in ovaries than testes of P. monodon broodstock. A homologue of ubiquitin specific proteinase 9, X chromosome (Usp9X) revealed a preferential expression level in ovaries than testes of broodstock-sized P. monodon (N = 13 and 11, P<0.05) but was only expressed in ovaries of 4-month-old shrimp (N = 5 for each sex).

• BMB Reports
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• 제39권1호
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• pp.37-45
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• 2006

Bulked segregant analysis (BSA) and AFLP were used for isolation of genomic sex determination markers in Penaeus monodon. A total of 256 primer combinations were tested against 6-10 bulked genomic DNA of P. monodon. Five and one candidate female- and male-specific AFLP fragments were identified. Female-specific fragments were cloned and further characterized. SCAR markers derived from FE10M9520, FE10M10725.1, FE10M10725.2 and FE14M16340 provided the positive amplification product in both male and female P. monodon. Further analysis of these markers using SSCP and genome walk analysis indicated that they were not sex-linked. In addition, sex-specific (or differential) expression markers in ovaries and testes of P. monodon were analyzed by RAP-PCR (150 primer combinations). Twenty-one and fourteen RAP-PCR fragments specifically/differentially expressed in ovaries and testes of P. monodon were successfully cloned and sequenced. Expression patterns of 25 transcripts were tested against the first stranded cDNA of ovaries and testes of 3-month-old and broodstock-sized P. monodon (N = 5 and N = 7 - 10 for females and N = 4 and N = 5 - 7 for males, respectively). Five (FI-4, FI-44, FIII-4, FIII-39 and FIII-58) and two (M457-A01 and MII-51) derived RAP-PCR markers revealed female- and male-specific expression patterns in P. monodon. Surprisingly, MII-5 originally found in testes showed a higher expression level in ovaries than did testes of juvenile shrimps but a temporal female-specific pattern in P. monodon adults.

• Structural Engineering and Mechanics
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• 제43권6호
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• pp.823-833
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• 2012

An experimental method was suggested for obtaining fracture toughness ($K_{Ic}$) and the tensile strength (${\sigma}_t$) of chopped strand glass fiber reinforced polymer concretes (PC). Semi-circular bend (SCB) specimens subjected to three-point bending were used for conducting the experiments on the PC material. While the edge cracked SCB specimen could be used to evaluate fracture toughness, the tensile strength was obtained from the un-cracked SCB specimen. The experiments showed the practical applicability of both cracked and un-cracked SCB specimens for using as suitable techniques for measuring $K_{Ic}$ and ${\sigma}_t$ in polymer concretes. In comparison with the conventional rectangular bend beam specimen, the suggested SCB samples need significantly less material due to its smaller size. Furthermore, the average values of ${\sigma}_t$ and $K_{Ic}$ of tested PC were approximately 3.5 to 4.5 times the corresponding values obtained for conventional concrete showing the improved strength properties of PC relative to the conventional concretes.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.3-10
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• 2014

The Japan National Committee for the Union for International Cancer Control (UICC) and UICC-Asia Regional Office (ARO) organized an international session as part of the official program of the 72nd Annual Meeting of the Japanese Cancer Association to discuss the topic "What is cost-effectiveness in cancer treatment?" Healthcare economics are an international concern and a key issue for the UICC. The presenters and participants discussed the question of how limited medical resources can be best used to support life, which is a question that applies to both developing and industrialized countries, given that cancer treatment is putting medical systems under increasing strain. The emergence of advanced yet hugely expensive drugs has prompted discussion on methodologies for Health Technology Assessment (HTA) that seek to quantify cost and effect. The session benefited from the participation of various stakeholders, including representatives of industry, government and academia and three speakers from the Republic of Korea, an Asian country where discussion on HTA methodologies is already advanced. In addition, the session was joined by a representative of National Institute for Health and Care Excellence (NICE) of the United Kingdom, which has pioneered the concept of cost-effectiveness in a medical context. The aim of the session was to advance and deepen understanding of the issue of cost-effectiveness as viewed from medical care systems in different regions.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10809-10812
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• 2015

Background: Human papillomavirus (HPV) DNA testing is an effective method to screen for precancerous changes in the cervix. Samples from self-collection rather than Pap smear can potentially be used to test for HPV as they are more acceptable and preferred for use in certain settings. The objective of this study was to compare HPV DNA testing from self-collected vaginal swabs and physician-collected cervical swabs. Materials and Methods: A total of 101 self-collected vaginal and physician-collected cervical swabs of known cytology from Thai women were tested by electrochemical DNA chip assay. The specimens were divided into 4 groups: 29 with normal cytology, 14 with atypical squamous cells of undetermined significance (ASCUS), 48 with low-grade squamous intraepithelial lesion (LSIL), and 10 with high-grade squamous intraepithelial lesion (HSIL). Results: Positive detection rates of HPV from self-collected swabs were similar to those from physician-collected swabs. Among specimens with abnormal cytology, HPV was found in 50% of self-collected swabs and 47.2% of physician-collected swabs. In specimens with normal cytology, 17.2% of self-collected swabs and 24.1% of physician-collected swabs were positive for HPV. Concordance was relatively high between results from self-collected and physician-collected samples. The most common HPV genotype detected was HPV 51. Conclusions: HPV DNA testing using self-collected swabs is a feasible alternative to encourage and increase screening for cervical cancer in a population who might otherwise avoid this important preventive examination due to embarrassment, discomfort, and anxiety.

• Journal of Microbiology and Biotechnology
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• 제21권9호
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• pp.954-959
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• 2011

There have been a number of studies conducted in order to compare the efficiencies of recovery rates, utilizing different protocols, for the isolation of L. monocytogenes. However, the severity of multiple cell injury has not been included in these studies. In the current study, L. monocytogenes ATCC 19112 was injured by exposure to extreme temperatures ($60^{\circ}C$ and $-20^{\circ}C$) for a one-step injury, and for a two-step injury the cells were transferred directly from a heat treatment to frozen state to induce a severe cell injury (up to 100% injury). The injured cells were then subjected to the US Food and Drug Administration (FDA), the ISO-11290, and the modified United States Department of Agriculture (mUSDA) protocols, and plated on TSAyeast (0.6% yeast), PALCAM agar, and CHROMAgar Listeria for 24 h or 48 h. The evaluation of the total recovery of injured cells was also calculated based on the costs involved in the preparation of media for each protocol. Results indicate that the mUSDA method is best able to aid the recovery of heat-injured, freeze-injured, and heat-freeze-injured cells and was shown to be the most cost effective for heat-freeze-injured cells.