• Development and Reproduction
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• v.21 no.1
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• pp.79-91
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• 2017

The aim of this study was to compare the efficacy of cryopreservation methods for ex situ conservation of spermatozoa from far eastern catfish, Silurus asotus. The spermatozoa activity index (SAI) and hatching rates were higher in spermatozoa stored in Alserver's solution than those of spermatozoa stored in glucose solution. The SAI and hatching rates in all experimental groups gradually decreased with increasing duration of storage. Additionally, the SAI and hatching rates gradually decreased with increasing thawing temperatures at all storage durations (P<0.05). Based on the SAI and hatching rates, our results suggest that the optimal cryopreservation conditions of catfish spermatozoa involve storage in Alserver's solution with 15% ethylene glycol, and thawing at $25^{\circ}C$. Cryopreservation of spermatozoa is a useful and reliable technique for conserving gene resources and for artificial propagation of far eastern catfish.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.45-48
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• 2006

혼합유류(등,경유)로 오염된 대상부지의 토양특성을 파악하여 세척공법의 적용성을 판단하고, 적용성시험을 통하여 장치별 설계인자를 도출하였다. 시험결과 본 세척대상토양의 경우 계면활성제와 같은 첨가제를 주입하지 않고 물리적인 탈착공정만을 거쳐도 90%이상의 오염물질이 토양입자표면으로부터 탈착됨을 알 수 있었으며, 이를 반영하여 장치를 제작, 설치하였다. 세척대상 오염토량은 총 $12,225m^3$, 사업수행기간은 약 6개월, 세척장치에 주입된 세척토양의 평균오염농도는 약 3,152ppm 이었으며 세척 완료된 토양의 평균농도는 약 150ppm으로 약 97%의 제거효율을 보여 복원목표인 800ppm을 만족시킬 수 있었다. 그리고, 세척토양의 입도분포를 정확히 파악하고 분리시킬 토양입자 크기를 결정하여 현장 적용한 결과 세척공법으로부터 배출되는 응집슬러지를 최소화할 수 있었으며, 발생되는 세척폐수 또한 세척수 처리시스템을 거쳐 재활용 할 수 있도록 하였다. 이런 결과를 통하여 세척공법이 다른 Ex-situ 공법과 비교하였을 경우 현장의 적용성, 경제성, 복원기간 등을 감안하였을 경우 성공적인 세척공법의 적용사례라 할 수 있다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.5
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• pp.566-570
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• 2018

This study determined the acoustic target strength (TS; dB) of black seabream Acanthopagrus schlegeli off the southern coast of Korea. For the ex-situ measurements, 200 kHz split beam transducers were used, and a Kirchhoff-ray mode (KRM) model acoustic model was used for the calculation. The fork length and total weight of the black seabream ranged from 6.4 to 30.8 cm and 6.4 to 683.8 g. respectively 200 kHz, the TS could beexpressed as a function of fork length as: $TS_{max}=20log_{10}(FL)-60.35(R=0.92)$ and $TS_{avg.}=20log_{10}(FL)-66.89(R=0.88)$. These TS results for black seabream can be used for estimating the biomass of fish in acoustic surveys in coastal areas.

• Progress in Superconductivity and Cryogenics
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• v.16 no.3
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• pp.4-6
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• 2014

Among Fe-based superconductors, potassium doped $BaFe_2As_2$ is favorable for applications because of its relatively high transition temperature and low anisotropy. To study the superconducting properties and the applicable aspects, high quality thin films of potassium doped $BaFe_2As_2$ should be fabricate. However, the high volatility of potassium makes it difficult to fabricate thin films of this compound. In this paper, we discuss the details of the experimental conditions used to fabricate $Ba_{1-x}K_xFe_2As_2$ films by ex situ PLD method. In the first set of samples, barium ratio in the target was controlled to make films with various potassium doping rate. However, in the second set of samples, the amount of potassium was controlled to find out optimal conditions for making high quality $Ba_{1-x}K_xFe_2As_2$ films.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.34-34
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• 2018

본 연구는 예산지역에서 현지외보전을 하는 매실나무(220여점) 및 살구나무(9점) 유전자원에 대한 개화 특성평가를 위하여 개화기를 지난 만개기를 연차별로 조사하였다. 대한민국에서 수집한 매실나무의 만개기는 이식 초기의 2012년에 4월 중순이었으나 이식 3년 후부터 4월 초순으로 약간 앞당겨졌다. 만개기는 2012년의 경우 모두 4월 11일 이후이었으나 2015년 이후는 4월 1~10일까지 70% 이상이었다. 연도별 보면 2015년과 2016년은 4월 1~5일에 가장 많이 만개하였고, 2017년과 2018년은 4월 6~10일에 가장 많이 만개하였다. 또한 일본에서 수집한 매실나무의 만개기도 대한민국 수집의 자원처럼 4월 초순이 대부분이었는데, 연도별로는 2015년, 2016년 및 2017년의 경우 4월 1~5일에 가장 많이 만개하였고, 2018년은 4월 6~10일에 가장 많이 만개하였다. 그러나 일본 수집의 매실나무 만개기는 3월말에서 4월 초순에 집중되어 3월 중순에서 4월 중순까지의 범위를 나타낸 대한민국 수집 자원과 약간의 차이를 보였다. 그리고 예산지역 현지외보전 살구나무(대한민국 수집)의 만개기는 2015년부터 2018년까지 모두 4월 1일부터 10일까지이었으며, 3월 및 4월 중순 이후에 만개한 꽃이 나타나지 않았다.

• Journal of Electrochemical Science and Technology
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• v.2 no.1
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• pp.8-13
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• 2011

Interfacial compatibility between the Si-Cu electrode and diluted ionic liquid electrolyte containing 50 vol.% of 1M lithium bis(trifluoromethanesulfonyl)imide (LiTFSI)/1-methyl-1-propylpyrrolidinium bis(trifluoromethylsulfonyl)imide (MPP-TFSI) and 50 vol.% dimethyl carbonate (DMC) in a lithium cell and dilution effect on surface chemistry are examined. ex-situ ATR FTIR analysis results reveal that the surface of the Si-Cu electrode cycled in the diluted ionic liquid electrolyte is effectively passivated with the SEI layer mainly composed of carboxylate salts-containing polymeric compounds produced by the decomposition of DMC. Surface species by the decomposition of TFSI anion and MPP cation are found to be relatively in a very low concentration level. Passivation of electrode surface with the SEI species contributes to protect from further interfacial reactions and to preserve the electrode structure over 200 cycles, delivering discharge capacity of > 1670 $mAhg^{-1}$ and capacity retention of 88% of maximum discharge capacity.

• Journal of Forest and Environmental Science
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• v.32 no.3
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• pp.311-322
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• 2016

Since forest trees form the basis of forest ecosystem, their prolong subsistence is crucial for various flora and fauna. The foremost challenges to sustain the forest ecosystem comprise of the declining forest tree population accompanied with structural changes due to afforestation and exploitation of forest area, environment changes, pests, pollution, and introgressive hybridization. For ex situ conservation approach, in vitro techniques encompass basic role for conserving tree genetic resources, predominantly where natural propagules like recalcitrant seed might not be appropriate for long-term conservation. The practice includes restricted growth techniques, conventional micropropagation, production and storage of synthetic seeds, and cryopreservation. Even though these practices have been applied chiefly to herbaceous species, but recently, woody species were also focused upon. Key conceptions, challenges and techniques for forest tree seed conservation are discussed briefly in this review with special emphasis on some successful cryopreservation approaches for long-term storage.

• Progress in Superconductivity and Cryogenics
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• v.16 no.2
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• pp.24-28
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• 2014

Magnesium diboride superconductor is still of considerable interest because of its appealing characteristics towards application mainly at around 20 K. Unlike Nb-based superconductors, $MgB_2$ can be operated by cryogen-free cooler which provides a cost effective alternative at low field of around 2-5 T. To explore this operating field region considerable efforts are necessary to marginally improve the superconducting properties of $MgB_2$. Under this situation, even the heat treatment environment during the synthesis is considered as an important factor. The addition of $H_2$ gas in small amount with Ar as a mixed gas during annealing has an adverse effect on the superconducting properties of $MgB_2$. It is although interesting to find that the presence of Mg vapor along with hydrogen during heat treatment results in the appreciable improvement in the flux pinning and the overall response of the critical current density for the ex-situ $MgB_2$ samples.

• Progress in Superconductivity
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• v.14 no.1
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• pp.34-38
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• 2012

The effect of graphene inclusion in the ex-situ $MgB_2$ was analyzed with the help of resistivity behavior and critical current density studies. Amount of graphene was systematically varied from 0% for pristine sample to 3% by the weight of $MgB_2$. Graphene that is considered as a good source of carbon was found to be intact without any significant carbon doping in $MgB_2$ structure as reveled by XRD measurements. There was no signature of graphene inclusion as far as the superconducting transition is concerned which remained same at 39 K for all the samples. The transition width being sensitive to defect doping remained more or less about 2 K for all the samples showing no variation due to doping. Although there was no change in the superconducting transition or transition width, the graphene doped sample showed noticeable decrease in the overall resistivity behavior with respect to decrease in temperature. The graphene inclusion acted as effective pinning centers which have enhanced the upper critical field of these samples.

• Journal of Hydrogen and New Energy
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• v.32 no.5
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• pp.308-314
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• 2021

Chemical durability issue in polymer electrolyte membranes has been a challenge for the commercialization of polymer electrolyte membrane fuel cells (PEMFCs). In this study, we proposed a manufacturing method of Nafion composite membrane containing a stable polyimide antioxidant to improve the chemical durability of the membrane. The thermal casting of the Nafion solution with poly (amic acid) induced polyimide reaction. We evaluated proton conductivity, oxidative stability with ex-situ Fenton's test, and fluoride ion emission to analyze the effect of polyimide antioxidants. We confirmed that incorporating the polyimide antioxidant improves the chemical durability of the Nafion membrane while maintaining inherent proton conductivity.