• Journal of Life Science
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• v.16 no.2 s.75
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• pp.253-258
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• 2006

Garlic is a perennial herb primarily distributed throughout the world. These plants are regarded as a medically and agricultural important crop in the world. The genetic relationships between cultivated and wild species were investigated at the population levels by constructing tree based on ISSR (inter-simple sequence repeats) markers. In addition, ISSR analysis was also conducted to estimate genetic diversity and population structure of these species. Three wild garlic populations in Korea were found to have more alleles per locus (mean 1.672 vs. 1.510) higher percent polymorphic locus (67.2 vs. 51.0), and higher diversity (0.250 vs. 0.198) than three cultivated populations. The cultivated and wild species in Korea are well separated from each other at phylogenetic trees. Although there is not direct evidence that A. victorialis is an ancestor of Korean A. sativum, there is a possibility that cultivated A. sativum in Korea has evolved from wild A. victorialis in Korea. Populations of A. victorialis may be useful in germ-plasm classification and evolutionary process.

• Journal of Animal Science and Technology
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• v.57 no.12
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• pp.44.1-44.9
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• 2015

Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.2
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• pp.161-168
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• 2015

An experimental study was conducted to scrutinize the influence of the frequency of an ultrasonic standing wave on the variation in the behavior of a methane-air premixed flame. The evolutionary features of the propagating flame were captured by a high-speed camera, and the macroscopic flame behavior, including the flame structure and local velocities, was investigated in detail using a post-processing analysis of the high-speed images. It was found that a structural variation and propagation-velocity augmentation of the methane-air premixed flame were caused by the intervention of the ultrasonic standing wave, which enhanced the combustion reaction. Conclusive evidence for the dependency of the flame behaviors on the driving frequency of the ultrasonic standing wave and equivalence ratio of the reactants is presented.

• Journal of Microbiology and Biotechnology
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• v.15 no.5
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• pp.1028-1038
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• 2005

To elucidate phylogenetic relationships of Phellinus and its related genera, nuclear internal transcribed spacer and mitochondrial small subunit ribosomal DNA sequences from 65 strains were determined and compared. The combined dataset of two sequences increased informative characters and led to the production of trees with higher levels of resolution. Phylogenetic analysis of the combined dataset revealed thirteen evolutionary lineages and several unresolved species that were together subdivided into two large clusters consisting of oligonucleate species and binucleate species. These results coincided with previous cytological, morphological, and molecular studies. It is newly recognized that the Phellinus linteus complex forms a sister clade to Inonotus, and that Fulvifomes is somehow related to Inocutis. The Phellinus linteus complex of dimitic perennial taxa made an independent clade from Inonotus and suggested that hyphal miticity and fruitbody permanence had enough phylogenetic significance to keep the complex within the traditional genus Phellinus. Taxa lacking setae were clustered into Fulvifomes, Phylloporia, Inocutis, and Fomitiporia, and the first three were closely related sister groups, but Fomitiporia was a genus distantly related to them. Several taxa with branched setae were shown among distantly related genera. Molecular evidence indicated that the ancestral nuclear type could be a binucleate feature, and that there might be parallel gains of branched setae and parallel losses of setae in the Hymenochaetales.

• The Korean Journal of Zoology
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• v.32 no.3
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• pp.232-241
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• 1989

Two cytosol malate dehydrogenase allelomorphs are found in the Dark Chub, Zacco temmincki, a freshwater fish inhabiting Korean waters. Mdh-1MM(hereafter referred to as A-type) is distributed along the south and southeastern parts of Korea, whereas the B-type(Mdh-1MS) is found everywhere except the southeast. Several syrnpatric populations can be found in the southern areas with no hybridization between them. The pattern of geographic distribution would indicate that A-type is superior adaptation to colder water temperature; this is collaborated by evidence collected from a sympatric length of the Tongchon stream, Namhae. The relative warmth of the water along this portion of the stream's length was determined its altitude and the time of year. Frequency of B-types found was directly proportional to the relative warmth of the water and inversely proportional to the nurnber of A-types found in the sarne station. Current data tends to suggest that the B-type of Zacco temrnincki first differentiated from Zacco platypus approximately one million years ago (Yang et al., 1984) while A-type subsequently branched from B-type 300,000 years later(Yang and Mm, 1987) through a progressive adaptation toward colder temperature.

• Korean journal of applied entomology
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• v.40 no.2
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• pp.125-129
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• 2001

Some wingless species have evolved take-off behaviors that enable them to become airborne. We examined aerodynamic attributes of dispersal relative to the body size and standing vs. walking postures for three phytoseiids that were suspected to have different take-off behaviors and dispersal abilities, Phytoseiulus persimilis Athias-Henriot, Neoseiulus fallacis (Carman) and N. californicus (McGregor). The average vertical profile of Pp in the walking position was significantly higher than those of Nf and Nc when in walking position. The body height of Nf in the standing posture was significantly greater than the body height of Pp when in the walking position. Cross-section areas also showed similar patterns of difference. Nf in the standing posture would have more than twice the drag force than in walking posture because of more fluid momentum in the wind boundary layer However, Pp in the walking position would have similar drag to Nf in the standing posture because of a higher vertical profile and larger size. Thus we add the scientific evidence of presence and absence of take-off behavior of some phytoseiid mites and evolutionary aspects of aerial dispersal are further discussed.

• The Korean Journal of Ecology
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• v.25 no.1
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• pp.1-7
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• 2002

The ecological background of a restoration project is complex and difficult to betermine without experimentation. A useful context for experiments is the well-studied process of natural succession, because the factors that drive or inhibit succession are also at work during reclamation (a form of primary succession) and restoration (which often resembles secondary succession). Using experimental studies on urban wasteland reclamation, we have tested for factors that stimulate or inhibit succession during early phases of woodland development in the Northeastern United states. The emphasis has been on mutualisms (seed dispersal, pollination, and mycorrhizae) and microsite limitations in the recruitment, growth, and reproduction of woody plants. Using plantings of seeds, seedlings, and clusters of reproductively mature plants on abandoned landfills, we have observed that (1) soil microsite deficiencies lead to very poor germination (<0.1$\%$) and seedling survival (<0.01$\%$) of most native species; (2) seed dispersal by birds is a significant and reliable source of woody plant recruitment; however (3) proximity effects are strong, with most (up to 95$\%$) of seed rain falling in the vicinity of planted clusters that are closest to putative seed sources; and (4) remnant natural woodlands are critical components of the recruitment process. To emphasize the last point, in one case, we found that the destruction of approximately 50$\%$ of nearby natural woodland vegetation led to a commensurate decline in seed rain. In another case, we found that the species richness of recruits was strictly limited by the species composition of nearby source plant communities, with no evidence of community enrichment by long distance dispersal over 5 years. We conclude from these results that the size and proximity of remnant natural populations are critical considerations when planning reclamation and restoration programs that rely on natural successional processes.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.65.2-65.2
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• 2010

We present four new transits of the planetary system TrES-3 observed between 2009 May and 2010 June. Among those, the third transit by itself indicates possible evidence for brightness disturbance, which could originate from a starspot or an overlapping double transit. A total of 107 transit times, including our measurements, were used to determine the improved ephemeris with a transit epoch of $2454185.910950\pm0.000073$ HJED (Heliocentric Julian Ephemeris Date) and an orbital period of $1.30618698\pm0.00000016$ d. We analyzed the transit light curves using the JKTEBOP code and adopting the quadratic limb-darkening law. In order to derive the physical properties of the TrES-3 system, the transit parameters are combined with the empirical relations from eclipsing binary stars and stellar evolutionary models, respectively. The stellar mass and radius obtained from a calibration using $T_{eff}$, log $\rho$ and [Fe/H] are in good agreement with those from the isochrone analysis within the uncertainties. We found that the exoplanet TrES-3b has a mass of $1.93\pm0.07\;M_{Jup}$, a radius of $1.30\pm0.04\;R_{Jup}$, a surface gravity of $28.2\pm1.1\;m\;s^{-1}$, a density of $0.82\pm0.06\;\rho_{Jup}$, and an equilibrium temperature of $1641\pm23K$.

• Journal of The Korean Astronomical Society
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• v.48 no.6
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• pp.343-355
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• 2015

There is much observational evidence that active star formation is taking place in the Hii regions Sh 2-255 – 257. We present a photometric study of this star forming region (SFR) using imaging data obtained in passbands from the optical to the mid-infrared, in order to study the star formation process. A total of 218 members were identified using various selection criteria based on their observational properties. The SFR is reddened by at least E(B −V ) = 0.8 mag, and the reddening law toward the region is normal (R_V = 3.1). From the zero-age main sequence fitting method it is confirmed that the SFR is 2.1 ± 0.3 kpc from the Sun. The median age of the identified members is estimated to be about 1.3 Myr from a comparison of the Hertzsprung-Russell diagram (HRD) with stellar evolutionary models. The initial mass function (IMF) is derived from the HRD and the near-infrared (J, J −H) color-magnitude diagram. The slope of the IMF is about Γ = −1.6 ± 0.1, which is slightly steeper than that of the Salpeter/Kroupa IMF. It implies that low-mass star formation is dominant in the SFR. The sum of the masses of all the identified members provides the lower limit of the cluster mass (169M_⊙). We also analyzed the spectral energy distribution (SED) of pre-main sequence stars using the SED fitting tool of Robitaille et al., and confirm that there is a significant discrepancy between stellar mass and age obtained from two different methods based on the SED fitting tool and the HRD.

• Molecules and Cells
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• v.43 no.1
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• pp.86-95
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• 2020

The red-crowned crane (Grus japonensis) is an endangered, large-bodied crane native to East Asia. It is a traditional symbol of longevity and its long lifespan has been confirmed both in captivity and in the wild. Lifespan in birds is known to be positively correlated with body size and negatively correlated with metabolic rate, though the genetic mechanisms for the red-crowned crane's long lifespan have not previously been investigated. Using whole genome sequencing and comparative evolutionary analyses against the grey-crowned crane and other avian genomes, including the long-lived common ostrich, we identified redcrowned crane candidate genes with known associations with longevity. Among these are positively selected genes in metabolism and immunity pathways (NDUFA5, NDUFA8, NUDT12, SOD3, CTH, RPA1, PHAX, HNMT, HS2ST1, PPCDC, PSTK CD8B, GP9, IL-9R, and PTPRC). Our analyses provide genetic evidence for low metabolic rate and longevity, accompanied by possible convergent adaptation signatures among distantly related large and long-lived birds. Finally, we identified low genetic diversity in the red-crowned crane, consistent with its listing as an endangered species, and this genome should provide a useful genetic resource for future conservation studies of this rare and iconic species.