• Asian-Australasian Journal of Animal Sciences
• /
• v.3 no.4
• /
• pp.319-322
• /
• 1990

The studies were conducted on 12 lactating animals comprising of six Karan Swiss (KS) cows and six Murrah buffaloes in second and third lactation. At the start of experiment the cows had completed on an average 51 days in lactation and were yielding an average of 15.1 kg milk a day. The buffaloes had completed 53 days in lactation and were yielding an average of 11.6 kg milk a day. At fortnightly intervals jugular blood samples were drawn at morning, noon, evening and night hours. Plasma growth hormone concentrations were highest during morning and thereafter decreased. In both the species there was a definite trend in the change of growth hormone concentrations during the day. In general growth hormone concentration decreased as the stage of lactation advanced. The overall average values of plasma growth hormone in cows and buffaloes were 2.95 and 2.48 ng/ml which were not statistically different. With the advancing lactation, the decline in milk yields in both the species was positively correlated with the growth hormone concentrations.

• Asian-Australasian Journal of Animal Sciences
• /
• v.11 no.4
• /
• pp.368-374
• /
• 1998

The study was conducted on six multiparous Murrah buffaloes which were earlier artificially induced into lactation. During the experimental period of 15 days, buffaloes were managed in a loose housing system. All the buffaloes were administered a single injection of bromocryptine (@ $100{\mu}g/kg$ body weight) subcutaneously in the neck region at 08:30 A.M., 50 days postpartum (early lactation). Blood samples were collected from four buffaloes for a period of 5 days before the administration of bromocryptine i.e. on days -5, -4, -3, -2, -1, on day of treatment (day 0) and thereafter daily for a period of 9 days i.e 1, 2, 3, 4, 5, 6, 7, 8 and 9 to determine the hormones and blood metabolites. Homogeneous milk samples from all the buffaloes were collected at morning and evening milkings on days coinciding with the days of blood sampling for analysis of milk constituents. Administration of bromocryptine resulted in a significant inhibition of plasma prolactin within 24 hrs of treatment, but the response in all the buffaloes was not uniform. The effect of bromocryptine on plasma prolactin hormone lasted for 1-4 days but Cortisol concentration were not altered. Administration of bromocryptine neither affected blood glucose nor plasma non-esterified fatty acids concentration. Irrespective of level of milk production from different buffaloes, there was no effect of bromocryptine on milk yield which indicated that prolactin is not required for milk secretion during early lactation in buffaloes. Milk constituents like fat, protein and lactose were not affected by bromocryptine may be due to no effect of bromocryptine of milk yield.

• Korean Journal of Agricultural Science
• /
• v.43 no.3
• /
• pp.394-400
• /
• 2016

The objective of this study was to estimate the environmental factors affecting milk ${\beta}$-hydroxybutyric acid (BHBA) and acetone (Ac) concentrations in Holstein cattle. A total of 264,221 test-day records collected from the Korea Animal Improvement Association (KAIA) during the period of 2012 to 2014 were used in this study. Analysis of variance (ANOVA) was performed to determine the factors significantly affecting ketone body concentrations. Parameters considered in the model were season of test, season of calving, parity, lactation stage, and milk collecting time (AM and PM). According to the ANOVA, the $R^2$ for milk BHBA and Ac were 0.5226 and 0.4961, respectively. 'Season of test' showed a considerable influence on ketone body concentration. Least square (LS) means for milk BHBA concentrations was the lowest ($39.04{\mu}M$) in winter while it increased up to $62.91{\mu}M$ in summer. But Ac concentration did not significantly change along with 'season of test'. The means of milk BHBA and Ac concentrations were high at first lactation stage, low around second lactation stage, and then gradually increased. Cows milked in the morning had lower mean BHBA and Ac concentrations ($48.49{\mu}M$ and $121.69{\mu}M$, respectively) in comparison to those milked in the evening ($53.46{\mu}M$ and $130.42{\mu}M$, respectively). The LS means of BHBA and Ac slightly increased over parities. These results suggest that proper maintenance of milk collection, herd management programs, and evaluation of ketone body levels in milk should be considered for the efficient management of resistance to ketosis.

• Journal of Animal Science and Technology
• /
• v.46 no.4
• /
• pp.517-524
• /
• 2004

The objective of this study was to investigate the environmental effects on duration time of milk flow, peak milk flow, and average milk flow in teats, and to estimate their relationships with milk yields in morning and evening milking, and to provide fimdamental information for the further study on their relationships with clinical mastitis and somatic cell in milk. A total of 6,768 milking records were studied in 72 Holstein cows. The influences of season, parity, lactation month, and milking interval on characteristics of milk flow considering in linear model were significant(p<.05). Duration of milk flow was longest at milking in fall, past first parity and second month of lactation, and with milking interval over than 13.5hrs. Average milk flow rate and peak milk flow rate were highest at milking in summer, past first parity and 8${\sim}$10 months of lactation, and with milking interval over than 13.5hrs. Milk flow rate was positively correlated to milk yield, and negatively correlated to the duration of milk flow. However duration milk flow was positively correlated to the milk yield with high level of correlation coefficient(+.60). For the establishment of optimum selection criteria on these traits, other aspects such as the udder health, disease and respective economic weights of milk flow characteristics in this study must be considered to develop the indices.

• Near Infrared Analysis
• /
• v.2 no.1
• /
• pp.59-66
• /
• 2001

Nowadays, medical diagnostics is efficiently supported by clinical chemistry and near infrared spectroscopy is becoming a new dimension, which has shown high potential to provide valuable information for diagnosis. The investigation was carried out to study the influence of mammary gland inflammation, called mastitis, on cow´s milk spectra and milk composition measured by near infrared spectroscopy (NIRS). Milk somatic cell counts (SCC) in milk were used as a measure of mammary gland inflammation. Naturally occurred variations with milk composition within lactation and in the process of milking were included in the experimental design of this study. Time series of unhomogenized, raw milk spectral data were collected from 3 cow along morning and evening milking, for 5 consecutive months, within their second lactation. In the time of the trial, the investigated cows had periods with mammary gland inflammation. Transmittance spectra of 258 milk samples were obtained by NIRSystem 6500 spectrophotometer in 1100-2400 nm region. Calibration equations for the examined milk components were developed by PLS regression using 3 different sets of samples: samples with low somatic cell count (SCC), samples with high SCC and combined data set. The NIR calibration and prediction of individual cow´s milk fat, protein, and lactose were highly influenced by the presence of mil samples from animals with mammary gland inflammation in the data set. The best accuracy of prediction (i.e. the lower SEP and the higher correlation coefficient) for fat, protein and lactose was obtained for equations, developed when using only “healthy” samples, with low SCC. The standard error of prediction increased and correlation coefficient decreased significantly when equations for low SCC milk were used to predict examined components in “mastitis” samples with high SCC, and vice versa. Combined data set that included samples from healthy and mastitis animals could be used to build up regression models for screening. Further use of separate model for healthy samples improved milk composition measurement. Regression vectors for NIR mild protein measurement obtained for “healthy” and “mastitic” group were compared and revealed differences in 1390-1450 nm, 1500-1740 nm and 1900-2200 nm regions and thus illustrated post-secretory breakdown of milk proteins by hydrolytic enzymes that occurred with mastitis. For the first time it has been found that monitoring the spectral differences in water bands at 1440 nm and 1912 nm could provide valuable information for inflammation diagnosis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.18 no.7
• /
• pp.973-979
• /
• 2005

The present study was planned to examine the effect of different feeding regimens on milk urea concentration and milk protein concentration. The objectives are to describe the diurnal variations of milk urea (MU) concentration and to predict plasma urea (PU) concentration from MU concentration. Six lactating Murrah buffaloes were distributed in two groups and were fed two different diets in a crossover design. The diets consisted of leguminous crops as diet 1 (berseem (Trifolium alexandrinum)+concentrate mixture 1+wheat straw)) and non-leguminous crops as diet 2 (oats (Avana sativa)+concentrate mixture 2+wheat straw). All the diets were isocaloric and isonitrogenous. Each diet was fed to the animals for a period of 28 days, followed by a 10 day gap to obviate the carry over effect of the previous diet and then a switch over to the other diet. Digestibility trials were conducted on the last 7 days of each feeding period. Milk samples were collected on day 3, 7, 10, 14, 17, 21, 24 and 28 of the feeding period and blood samples were collected on the same day at morning within 30 minutes after morning milking. The average milk urea (MU) values (mg/dl) differed significantly (p<0.01) and were 44.83${\pm}$0.62 and 42.53${\pm}$0.73, respectively, for diets 1 and 2. Milk urea concentrations (mg/dl) also varied (p 0.01) among the days of feeding period, but were stabilized after 10th day of feeding period. In contrast, diets and days of feeding period had no significant effect on percent milk protein. Plasma urea concentration showed a significant (p<0.01) positive correlation (r = 0.93) with MU concentration. To predict the PU from MU the following equation was developed 'PU = 10.67${\pm}$0.76${\times}$MU (mg/dl) with $R^2$ = 0.87'. A clear diurnal variation of MU was found with lowered morning value (42.04${\pm}$0.68 mg/dl) than the evening value (45.32${\pm}$0.66 mg/dl). Present findings suggested that MU or PU concentration could be used as an indicator to monitor the feeding strategy. Plasma urea can be predicted from MU, whenever interpretation of milk urea data required consideration of diurnal variation.

• Journal of Animal Environmental Science
• /
• v.8 no.3
• /
• pp.159-164
• /
• 2002

The distance between teats of each head on twenty heads of holstein was measured in Nation Livestock Research Institute of Rural Development Administration to find design variables on the teat-cup attachment system of the auto-milking system before milking. The distance between teats was tested by the steps for milk producing in the morning and evening. The results from this study is summarized as follow. 1 The maximum and minimum length between front teats were 297mm and 112mm respectively, the maximum and minimum length between rear teats were 231mm and 36mm and the maximum and minimum length between left front and left rear were 220 and 84mm. And the maximum length of right front and right rear were 205mm and 90mm. A relative position of the each teats was asymmetric. 2. The size of teats, the length between front teats, and the length between rear teats by lactating period were very much changed for its milking. 3. The design variables on the teat-cup attachment system was found by the length between each teats tested. Since the position of teat-cup is changed by milking environment for a cow to milk, the design variables should be considered to be asymmetric area between four teat of COW.

• Journal of Animal Science and Technology
• /
• v.48 no.4
• /
• pp.487-492
• /
• 2006

This study estimated characteristics and genetic parameters for milking traits in Holstein dairy cattle. Daily records (12,561) were available from a hundred fifty four lactating cows with sixty six sires at National Livestock Research Institute from November, 2005 to April, 2006. The cows were fed same rations, and were milked twice daily using milking parlour equipped with Alpro-system?? to collect milking records automatically. The experimental cows have averaged 1.9 parities, 139.7 days in milk, and 13kg milk yield per milking. Average milk flow was 64% of peak milk flow. Milking duration, peak milk flow, average milk flow, and milk yield per milking were 5.57±1.67 minutes, 3.58±0.79kg/minute, 2.28±0.51kg/minute, and 13.02±4.09kg, respectively. All traits in the morning milking were higher than those of in the evening milking, and were also increased with parity. Heritabilities for milking duration, peak and average milk flow, and milk yield per time were 0.49, 0.70 0.58 and 0.36, respectively. The genetic correlations of milking duration with peak milk flow, average milk flow, and milk yield per time were 0.48, 0.54, and 0.41, respectively. The correlations of milk yield per milking with peak milk flow and average milk flow were 0.23 and 0.30, respectively. In conclusion, milk flow was increased with milk yield; however milk flow was opposite relationship with milk duration. Further studies are not only necessary to warranty genetic parameters, but pre-installation of automatic recording system to collect daily milking record is also necessary.

• Asian-Australasian Journal of Animal Sciences
• /
• v.7 no.1
• /
• pp.63-68
• /
• 1994

432 samples of raw goat milk were analyzed for sodium, calcium, potassium, chloride, and ashes content. The samples came from 16 pure breed goats, 2 to 4 years of age kept in semi-feedlots. They were basically fed soy bran, cotton bran, triturated Guandu hay and disintegrated corn with its straw and corncob (according to requirements for due preservation and production). The mean values found were: sodium, $45{\pm}10.2mg%$ (24.5-65.6 mg%); potassium, $206{\pm}44.7mg%$ (116.6-295 mg%); calcium, $111{\pm}22mg%$ (66.5-155.4 mg %); ashes, $0.82{\pm}0.06mg%$ (0.68-0.95%); chloride, $235{\pm}39mg%$ (157-313 mg%). Results were related to: a) breed, the Brown and the Saanen revealed superior values for calcium and ashes, b) climate, which in the rain season led to higher contents of sodium, potassium, and calcium when compared to the local dry season, c) time of lactation which increasingly affected the contents of calcium, sodium and ashes, and d) the time between milking, when higher concentration of chloride and ashes were mostly concentrated in the evening and calcium in the morning. These results have important implications in the handling and management of the goat herd.

• Journal of the Korean Society of Food Culture
• /
• v.5 no.2
• /
• pp.217-228
• /
• 1990

An ecological study of food and nutrition was conducted among 198 children, 6 to 12 years old, of the Songmyun elementary school and Samsong elementary school, located in remote villages of Chungchun-myun, Goisan-gun, Chung Buk Province between July 11 and 16, 1988. The results were summarized as follows. In regard to frequency of skipping breakfast, 3.3% of subjects skipped every morning; 15% often skipped; and the others never skipped. The most common reason for skipping a meal was lack of appetite. 6.6% of subjects often skipped an evening meal because of delayed meal preparation by a mother who returned home late. The survey found that 5.1% of subjects skipped a lunch at school. The reasons for skipping lunch were the mother’s being too busy to prepare a boxed lunch; a school bag too heavy to carry for a long distance to school; no adequate side-dishes for the boxed lunch. Growth patterns of those children having poor food habits, were reflected in lower figures in arm circumference and weight for height. The percentage of children having meals with their family on weekdays was 84.7% for breakfast; 85.6% for evening meal. About 7% of subjects had many dislikes and 74.1% had some dislikes in what they ate; 18.9% had good food preferences. There was a strong correlation between appetite or food preferences and anthropometric measurements or nutrient intake. Boiled rice was the predominant dietary staple, and principal sources of protein taken by subjects at home were soy beans and soy products. Regarding the boxed lunch prepared at home, most popular was one with only one kind of side-dish, including kimchee. 43.4% of the side-dishes were prepared with vegetables only. As for between meal snacks, milk and milk products, fruits and breads were popular among the subjects. The survey found that the children liked fruits, yogurt, corn, Chajang noodle; in contrast, lowest preference was for soup made with bones, curried meat with rice, and aromatic vegetables. The survey discovered a local dietary characteristics; 46.2% of the children customarily ate frog meat, an unconventional food.