• International Journal of Industrial Entomology and Biomaterials
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• 제43권1호
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• pp.6-15
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• 2021

We investigated the taxonomic status of the Korean populations of Eurema hecabe (Linnaeus, 1758) based on morphology and nuclear triose phosphate isomerase (Tpi). Up to now, the Korean population of E. hecabe was classified into E. mandarina (de l'Orza, 1869) based on morphological characteristics. A previous study raised the possibility of the Jeju-do population to be E. hecabe based on the color of forewing's fringe. However, morphological examination showed that the Korean population found in southern areas (Gyeonsang and Jeolla provinces), including Jeju-do is E. mandarina. In particular, the external morphology of autumn seasonal type of the Korean population was almost same as the Japanese mainland population, which is known as E. mandarina. Furthermore, sequence analysis of Tpi gene from nine individuals of E. mandarina collected in South Korean localities including Jeju-do clearly showed that all Korean specimens truly belong to E. mandarina.

• International Journal of Industrial Entomology and Biomaterials
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• 제31권2호
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• pp.35-39
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• 2015

The developmental responses of insects to temperature are important considerations in gaining a better understanding of their ecology and life histories. Temperature-dependent phenology models permit examination of the effects of temperature on the geographical distributions, population dynamics, and management of insects. Measurements of insect developmental and survival responses to temperature pose practical challenges that depend on the chosen modality, variability among individuals, and high mortality rates near the lower and upper threshold temperatures. Different temperature levels can significantly affect larval development of Eurema hecabe. The development of E. hecabe reared on leaves of Lespedeza cuneata was investigated at three temperature regimes (20, 25, and 30℃), a relative humidity of 60%, and a light:dark photoperiod of 14:10 h. The developmental time from larva to adult was 34.3, 20.6, and 17.9 d at temperatures of 20, 25, and 30℃, respectively. Pupal rate was 47.6%, 47.6%, and 61.9% at temperatures of 20, 25, and 30℃, respectively. The developmental threshold temperature estimated from larva to pupae was 8.1℃ with 381.7 degree-days. There is an increasing need for a standardized manual for rearing this butterfly species based on adequate knowledge of its ecology.

• 한국잠사곤충학회지
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• 제50권2호
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• pp.133-139
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• 2012

본 연구는 남방노랑나비의 인공사육을 위한 서식지의 생태환경조사와 실내에서의 인공사육조건을 구명하기 위해 수행되었다. 서식지의 생태환경조사를 위해 남방노랑나비의 주 서식지인 경남 사천시 곤양면 대진리(A지역, 하천변의 개활지)와 남해군 설천면 노량리(B지역, 구릉성 산자락) 두 곳을 조사지로 선정하여, 2010년 6월부터 2010년 11월까지 6개월간 월 2회 정기적으로 조사하였다. 조사결과, 산자락인 B지역의 남방노랑나비의 개체수가 개활지인 A지역보다 성충의 경우 6배 더 많은 것으로 나타났다. 또한 알, 애벌레, 번데기도 더 많은 수가 조사되었다. B지역의 경우 먹이식물이 다양하게 분포하였으며, 먹이식물의 선호도는 비수리에서 170개체가 관찰되어 자연에서는 비수리가 가장 선호하는 기주식물임이 확인되었다. 실내 사육조건을 구명하기 위해 남방노랑나비의 실내 사육을 실험하였다. 기주식물에 따른 산란선호도를 조사하기위해 암 수 100개체를 산란장에 넣고 짝짓기를 유도한 후 비수리와 결명자를 넣고 10분간 산란한 알의 수를 세어 산란선호도를 조사한 결과 비수리에 산란한 알의 수가 $104.9{\pm}19.6$개로 결명자의 $12.7{\pm}4.5$개 보다 많아 비수리의 산란선호도가 높게 나타났다. 산란된 알을 고온 장일 조건($25^{\circ}C$, 16L : 8D)의 인공적인 환경에서 사육한 결과, 산란된 알의 부화율은 53.7%이었으며, 산란에서 부화까지 평균 소요일수는 $5.1{\pm}0.9$일이었다. 유충기간은 $12.1{\pm}0.9$일이었으며, 각 령별 두폭측정 결과 1령 $0.36{\pm}0.02$, 1령 $0.61{\pm}0.02$, 3령 $0.93{\pm}0.05$, 4령 $1.46{\pm}0.08$, 5령 $2.25{\pm}0.11$로 나타났다. 사육된 애벌레의 용화율은 81.0%으로 나타났으며, 번데기기간은 평균 $6.9{\pm}0.7$일로 조사되었다. 나비의 우화율은 79.6%으로 나타났다. 이상의 실험결과 남방노랑나비의 자연상태에서의 서식환경과 실내 인공사육을 위한 사육조건이 확인되었다.

• 한국식품저장유통학회지
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• 제17권5호
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• pp.752-756
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• 2010

나비류 추출물은 전통적으로 다양한 활성을 보유하여 의약용으로 사용되어졌다. 5종의 나비(산제비나비[Papilio maackii], 호랑나비[Papilio xuthus], 배추흰나비[Pieris rapae], 남방호랑나비[Eurema hecabe], 왕오색나비[Sasakia charonda])를 이용하여 물, dimethly sulfoxide (DMSO), ehtanol 및 methanol로 추출한 추출물로 항산화 활성 및 Cox-2 promoter assay를 수행하였다. 그 결과 산제비나비의 추출물이 전반적으로 높은 항산화활성을 나타내었으며, Cox-2 promoter assay에서는 호랑나비의 DMSO 분획이 가장 저해활성이 높은 것으로 나타났다. 활성이 높은 추출물을 대상으로 분리 및 정제를 통하여 유용한 식의약 소재로의 기초연구가 더 필요하다.

• Journal of Microbiology and Biotechnology
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• 제25권9호
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• pp.1410-1416
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• 2015

Wolbachia is an obligate symbiotic bacteria that is ubiquitous in arthropods, with 25-70% of insect species estimated to be infected. Wolbachia species can interact with their insect hosts in a mutualistic or parasitic manner. Sequence types (ST) of Wolbachia are determined by multilocus sequence typing (MLST) of housekeeping genes. However, there are some limitations to MLST with respect to the generation of clone libraries and the Sanger sequencing method when a host is infected with multiple STs of Wolbachia. To assess the feasibility of massive parallel sequencing, also known as next-generation sequencing, we used pyrosequencing for sequence typing of Wolbachia in butterflies. We collected three species of butterflies (Eurema hecabe, Eurema laeta, and Tongeia fischeri) common to Korea and screened them for Wolbachia STs. We found that T. fischeri was infected with a single ST of Wolbachia, ST41. In contrast, E. hecabe and E. laeta were each infected with two STs of Wolbachia, ST41 and ST40. Our results clearly demonstrate that pyrosequencing-based MLST has a higher sensitivity than cloning and Sanger sequencing methods for the detection of minor alleles. Considering the high prevalence of infection with multiple Wolbachia STs, next-generation sequencing with improved analysis would assist with scaling up approaches to Wolbachia MLST.

• 농촌계획
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• 제21권4호
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• pp.1-12
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• 2015

Various types of parks such as ecological park, dragonfly park, etc. are tried to design in order to resolve different environmental issues in urban areas. Parks are considered organism habitats that not only improve the biodiversity but also give people a chance to observe living organisms in urban area. This study was conducted to select basic materials for planning a butterfly garden through reference reviews. The following 21 species of butterflies belonged to 1 order, 4 families, and 16 genera were selected: Phengaris teleius, Pseudozizeeria maha, Thecla betulae, Argynnis hyperbius, Dichorragia nesimachus, Hestina assimilis, Polygonia caureum, Sasakia charonda, Vanessa cardui, Vanessa indica, Boloria selene, Byasa alcinous, Papilio machaon, Papilio macilentus, Papilio protenor, Papilio xuthus, Papilio bianor, Sericinus montela, Colias erate, Eurema hecabe, and Pieris rapae. 85 species belonged to 22 families, 59 genera, 74 species, 10 varieties, and 1 subspecies were selected for host and nectar plants for butterflies selected above. These results would be expected to useful in butterfly gardening. After that, we think it is necessary to be applied these study results in field. This study requires a test in the butterfly garden. The test results will be make the butterfly-plant matrix.