• 제목/요약/키워드: Euphorbia humifusa Willd

검색결과 4건 처리시간 0.017초

Identification of the active components inhibiting the expression of matrix metallopeptidase-9 by TNFα in ethyl acetate extract of Euphorbia humifusa Willd

  • Ahn, Seunghyun;Jung, Hyeryoung;Jung, Yearam;Lee, Junho;Shin, Soon Young;Lim, Yoongho;Lee, Young Han
    • Journal of Applied Biological Chemistry
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    • 제62권4호
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    • pp.367-374
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    • 2019
  • Euphorbia humifusa Willd (EuH), called Ttang-Bin-Dae in Korea, is a traditional medicinal plant widely used for its anti-inflammatory and antiviral activity. Ethyl acetate (EA) extracts of EuH (EA/EuH) inhibit invasion and metastasis by inhibiting tumor necrosis factor TNFá-induced matrix metalloproteinases (MMP)-9 expression in human breast cancer cells. However, the bioactive components of EA/EuH mediating the inhibition of MMP-9 expression have not been identified. In the present study, three bioactive constituents of EA/EuH were isolated using high-performance liquid chromatography. Nuclear magnetic resonance spectroscopy revealed isoquercetin, avicularin, and astragalin as the bioactive compounds responsible for preventing TNFα-induced MMP-9 mRNA expression in breast cancer cells. These findings suggest that isoquercetin, avicularin, and astragalin could be used as valuable anti-metastatic agents against metastatic cancers.

In vitro Adipocyte Differentiation Inhibition and in vivo Effects on Lipid Metabolism in High-Fat Diet-Induced Obesity of Euphorbia humifusa

  • Sung-Gyu Lee;Hyun Kang
    • Journal of Microbiology and Biotechnology
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    • 제34권2호
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    • pp.387-398
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    • 2024
  • Euphorbia humifusa Willd (Euphorbiaceae) is a functional raw material with various pharmacological activities. This study aimed to validate the inhibitory effect of Euphorbia humifusa extract (EHE) on adipocyte differentiation in vitro and in a high-fat-diet (HFD)-induced mouse model to evaluate the E.a humifusa as a novel anti-obesity and lipid metabolism enhancer agent. EHE effects on obesity and lipid metabolism were assessed in HFD-induced obese mice after 4-week treatments. Results were compared among four treatment groups (n = 7/group): low fat diet (LFD), high fat diet (HFD), and HFD-induced obese mice treated with either 100 or 200 mg/kg/day EHE (EHE100 and EHE200, respectively). EHE (50 to 200 ㎍/ml) and quercetin (50 ㎍/ml) significantly reduced 3T3-L1 preadipocyte differentiation (p < 0.001), in a concentration-dependent manner. EHE affected lipid metabolism, as evidenced by changes in serum lipid components. The HFD-EHE100 and HFD-EHE200 groups exhibited significantly (p < 0.05) reduced triglycerides (TG, 97.50 ± 6.56 and 82.50 ± 13.20 mg/dL, respectively) and low-density lipoprotein-cholesterol (LDL-c: 40.25 ± 4.99 and 41.25 ± 6.36 mg/dL, respectively) compared to the HFD group (TG: 129.25 ± 19.81 mg/dL; LDL-c: 51.75 ± 11.59 mg/dL). Haematoxylin and Eosin (H&E) and Oil red O staining showed that EHE markedly reduced lipid accumulation and inhibited lipogenesis in the liver. Interestingly, EHE significantly (p < 0.01) reduced the expression of adipogenic transcription factors in liver tissue. Our results indicated that EHE has the potential to be a therapeutic agent for addressing obesity and lipid metabolism.

한국산 대극속(Euphorbia L., Euphorbiaceae)의 세포분류학적 연구 (Cytotaxonomic study of Korean Euphorbia L. (Euphorbiaceae))

  • 정규영;오병운;박기룡;김주환;김미숙;남기흠;장창기
    • 식물분류학회지
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    • 제33권3호
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    • pp.279-293
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    • 2003
  • 한국산 대극속(Euphorbia L.) 13종의 체세포염색체의 분류학적 가치를 평가하기 위하여 본 연구를 수행하였다. 본 연구에 취급된 분류군들의 체세포염색체수는 2n= 12, 20, 22, 28, 40, 42, 56이었고, 기본염색체수는 x=6, 7, 10, 11이었다. 본 연구에서 체세포염색체수가 처음으로 밝혀진 분류군은 낭독(E. pallasii Turcz.; 2n=20), 단주낭독(E. hylonoma Hand.-Mazz; 2n=20.), 두메대극(E. fauriei H. L$\acute{e}$v. & Vaniot ex H. L$\acute{e}$v.;2n=28), 암대극(E. jolkini Boiss.; 2n=28)의 4종류이었으며, 기존의 보고와 일치하는 분류군은 개감수(E. sieboldiana Moor. & Decne.; 2n=20), 붉은대극(E. ebracteolata Hayata; 2n=20), 땅빈대(E. humifusa Willd. ex Schlecht.; 2n=22)의 3종류이었다. 반면 기존의 보고와 다르게 나타난 종류는 흰대극(E. esula L.; 2n= 16, 20, 60, 64 vs 2n=20), 등대풀(E. helioscopia L.; 2n=12, 42 vs 2n=42), 참대극(E. lucorum Rupr.; 2n=28, 40 vs 2n=56), 대극(E. pekinensis Rupr. in Maxim.; 2n=24 vs 2n=28, 56), 큰땅빈대(E. maculata L.; 2n=28, 42 vs 2n=12), 애기땅빈대(E. supina Raf.; n=7 vs 2n=40)이었다. 낭독, 붉은대극, 단주대극은 염색체의 수와 크기 및 부수체의 존재에 의해 다른 분류군들과 뚜렷히 구분되었으며, 큰땅빈대, 땅빈대, 애기땅빈대는 외부형태, 해부, 화분학적 형질의 공통성에도 불구하고 기본염색체수 및 체세포염색체수에서 차이를 보였다. 체세포염색체에서 얻어진 결과는 Webster(1967)의 분류체계보다 Ma and Hu(1992)의 분류체계가 타당한 것으로 판단되었다. 이와 같이 본 연구에서 얻어진 체세포염색체에 관한 형질은 절 수준에서 분류학적으로 유용하게 적용되었으며, 일부 종의 유연관계를 파악하는데 그 유용성이 인정되었다.

국내 유용식물의 항산화 효과 (Antioxidative Effect of Domestic Plants)

  • 이성규;이은;최무영;임태진;박희준;차배천
    • 생약학회지
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    • 제28권1호
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    • pp.15-20
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    • 1997
  • This study was carried out to investigate the antioxidative activities of domestic plants grown in the area of Kangwon-do province. Through the examination of methanol extracts from 15 plants for radical scavenging effects using DPPH method, the extracts from Pinus densiflora Sieb. et Zucc. And Euphorbia humifusa Willd. Showed strong antioxidative activity. Because of its highest antioxidative activity among 15 domestic plants, radical scavenging effects of four different extract compartments from leaves of P.densiflora, n-Hexane, EtOAc, BuOH and $H_2O$ extracts. Were examined by DPPH method. Antioxidative activities of EtOAc and BuOH extracts were similar or even higher than that of natural (tocopherol) or synthetic antioBfdants (BHA) , suggesting that major fraction for the antioxidative activity of P. densiflora was the EtOAc and BuOH extract compartments.

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