• Archives of Pharmacal Research
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• v.28 no.11
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• pp.1287-1292
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• 2005

KR-33028 (N-[4-cyano-benzo[b]thiophene-2-carbonyl]guanidine) is a new cardioprotective agent for preventing ischemia-reperfusion injury. This study was performed to identify the metabolic pathway of KR-33028 in human liver microsomes and to compare its metabolism with that of cryopreserved human hepatocytes. Human liver microsomal incubation of KR-33028 in the presence of NADPH and UDPGA resulted in the formation of four metabolites, M1, M2, M3, and M4. M1 and M2 were identified as 5-hydroxy-KR-33028 and 7-hydroxy-KR-33028, respectively, on the basis of LC/MS/MS analysis with the synthesized authentic standard. M3 and M4 were suggested to be dihydroxy-KR-33028 and hydroxy-KR-33028-glucuronide, respectively. Metabolism of KR-33028 in cryopreserved human hepatocytes resulted in the formation of M1, M2, and M4. These data show a good correlation between major metabolites formed in human liver microsomes and cryopreserved human hepatocytes. In addition, KR­33028 was found to inhibit moderately the metabolism of CYP1A2 substrates. Based on the results obtained metabolic pathway of KR-33028 is proposed.

• The Journal of the Petrological Society of Korea
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• v.26 no.4
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• pp.327-339
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• 2017

The Cretaceous Myeongseongsan Granite in the northwestern Gyeonggi Massif consists of a major pale pink-colored biotite monzogranite and a minor white-colored biotite alkaligranite. Low Sr and high Ba concentrations, negative Eu-anomalies in REE plot, negative Sr anomalies in spider diagram, a negative correlation between Sr and Rb, and positive correlations between Sr and Ba and $Eu/Eu^*$ indicate that a fractional crystallization of both plagioclase and K-feldspar played a significant role during magma evolution. The Myeongseongsan Granite is plotted in I-& S-type granites on I, S, A-type granite classification scheme. While the biotite monzogranite is plotted in unfractionated I-& S-type granite, the biotite alkaligranite is plotted in fractionated I-& S-type granite, which indicates that the biotite alkaligranite is a more differentiated product. In order to elucidate the nature of the protoliths of the peraluminous Myeongseongsan magma, we plotted in $Al_2O_3/TiO_2$ vs. $CaO/Na_2O$ and Rb/Sr vs. Rb/Ba diagrams, and they suggest that the Myeongseongsan Granite was derived from clay-poor metagreywackes and meta-psammites or their igneous counterparts. Whole-rock zircon saturation temperature indicates that the Myeongseongsan magma was melted at $740-799^{\circ}C$.

• Korean Journal of Community Nutrition
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• v.17 no.2
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• pp.167-181
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• 2012

Our society is aging rapidly, and the number of elderly people who are in charge of purchasing and preparing foods at home is increasing. However, most the elderly have difficulty managing nutrition and food safety by themselves. The purpose of this study was to develop the necessary knowledge, through focus group interview and Delphi technique to establish a food safety and nutrition education program. The diet and educational needs of the elderly were surveyed through FGI. The education topics were decided by the Delphi technique. The education program consisted of a five week program, and the topics taught were 'Dietary Change for Healthy Life', 'Prevention of Food Poisoning in Everyday Life', 'Safe Food Handling for my Health', 'Healthy Dietary Life to Prevent Chronic Disease', and 'Safety! Nutrition! Healthy Dietary Life'. This education program was designed to decrease the perceived barriers, and to increase the perceived interests and the sense of self-efficacy. Education program materials, lesson plans, slides, handouts, videos, leaflets, and booklets were developed. Based on the results, the contents of the food safety guideline leaflets for the elderly were decided as the following: (1) wash your hands in the correct way; (2) select safe food; (3) cook foods safely; (4) keep foods safely; (5) keep kitchen utensils clean. In conclusion, if advanced education programs are implemented and delivered continuously in locations such as health centers and community welfare centers, those will contribute significantly to enhance the perception of food safety and to change the desirable dietary behavior of the elderly.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.29-33
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• 2001

This study was carried out to discriminate potato cultivars and breeding lines by specific molecular markers using random amplified polymorphic DNA (RAPD) analysis. The genotypes of potatoes used for analysis were eight cultivars and five breeding lines. Some of those show much phenotypic resemblances among them because 'Jopung', 'Daekwan70', 'Gawon', and 'Daekwan72' have immediate parental relationship with 'Superior', 'Irish Cobbler', 'Namsuh', and 'Atlantic', respectively. So, there are many difficulties to distinguish the varieties by the morphological characteristics. Three URP primers, URP2, URP4, and URP8 were selected for promising primers to discriminate potato genotypes or cultivars. The three URP primers were shown very high reproducibility because of the relatively high annealing temperature and long primer size. Although the results of similarity analyses did not always reflect the genetic relationship between potato varieties, the reproducible pattern of amplified DNA bands by URP primers showed possibility for molecular markers for discrimination of potato genotype or cultivar.

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.434-440
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• 2019

Lactic acid bacteria (LAB) isolated from kimchi were studied for their ${\beta}$-glucuronidase activity. Among the 156 strains tested, 52 strains utilized glucuronic acid as a carbon source and their intracellular ${\beta}$-glucuronidase activities were significantly higher than their extracellular activities. Leuconostoc mesenteroides KFRI 73007 isolated from turnip kimchi exhibited the highest intracellular ${\beta}$-glucuronidase activity of $0.77{\pm}0.01U/mg$ protein, which was further increased to $1.14{\pm}0.01U/mg$ protein under optimized reaction conditions (pH 7, $37^{\circ}C$). The activity of ${\beta}$-glucuronidase was notably decreased by the addition of divalent cations, and glucuronic acid was the best carbon source to produce ${\beta}$-glucuronidase in Leu. mesenteroides KFRI 73007.

• IMMUNE NETWORK
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• v.4 no.1
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• pp.16-22
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• 2004

Background: To develop a novel treatment strategy for hepatitis B virus infection, a major cause of liver chirosis and cancer, we aimed to make human monoclonal antibodies inhibiting RNase H activity of P protein playing in important role in HBV replication. In this regard, phage display technology was employed and demonstrated as an efficient cloning method for human monoclonal antibody. So this study analysed the usability of human monoclonal antibody as protein based gene therapy. Methods: RNase H of HBV was expressed as fusion protein with maltose binding protein and purified with amylose resin column. Single chain Fv (scFv) phage antibody library was constructed by PCR cloning using total RNAs of PBMC from 50 healthy volunteers. Binders to RNase H were selected with BIAcore 2000 from the constructed library, and purified as soluble antibody fragment. The affinity and sequences of selected antibody fragments were analyzed with BIAcore and ABI automatic sequencer, respectively. And finally RNase H activity inhibiting assay was carried out. Results: Recombinant RNase H expressed in E. coli exhibited an proper enzyme activity. Naive library of $4.46{\times}10^9cfu$ was screened by BIAcore 2000. Two clones, RN41 and RN56, showed affinity of $4.5{\times}10^{-7}M$ and $1.9{\times}10^{-7}M$, respectively. But RNase H inhibiting activity of RN41 was higher than that of RN56. Conclusion: We cloned human monoclonal antibodies inhibiting RNase H activity of P protein of HBV. These antibodies can be expected to be a good candidate for protein-based antiviral therapy by preventing a replication of HBV if they can be expressed intracellularly in HBV-infected hepatocytes.

• Journal of Life Science
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• v.19 no.2
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• pp.271-276
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• 2009

The Purpose of this study was to examine the effects of boxing aerobic training on resting heart rate variability (HRV) in females. The subjects for this study were performed 16 female college students that aged 19-23. The subjects were divided into two groups; boxing aerobic exercise group (9 students) and control group (7 students). Boxing aerobic training program was performed three times a week for twelve weeks with $VO_{2max}$ 60-80% exercise intensity. The results of this study were following; 1. Mean HRT (mean heart rate) had no signigicant difference among the groups and exercise time. 2. SDNN (standard deviation of the normal to normal interval) had no significant difference among the group and exercise time. 3. RMS-SD (square root of the mean squared differences of successive normal to normal interval) had no significant difference among the groups and exercise time. 4. TP (total power) had no significant difference among the groups and exercise time. 5. LF (low frequency) had no significant difference among the groups and exercise time. 6. HF (high frequency) had no significant difference among the groups and exercise time. 7. LF/HF (low frequence/high frequency ratio) had no significant difference among the groups and exercise time. 8. VLF (very low frequency) had no significant difference among the groups and exercise time.

• Biomolecules & Therapeutics
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• v.5 no.1
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• pp.36-42
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• 1997

To investigate effects of ginseng total saponin (GTS) on the ethylcholine aziridnium ion (AF64A) -induced glutamatergic nervous system, rats were pretreated with the infusion of AF64A (3 nmole) into lateral ventricle and were posttreated with 50 mg/kg of GTS, i.p., for 1 week. Twenty four hours after the last administration, rats were sacrificed and the levels of glutamate and taurine, [$^3$H]dizocilpine ([$^3$H]MK801) binding sites and glutamine synthetase activity were assessed in striatum, hippocampus and frontal cortex. The levels of striatal glutamate after GTS treatment in rats were decreased. And the levels of glutamate were decreased in striatum and frontal cortex and increased in hippocampus by the infusion of AF64A. However, the AF64A-induced changes of glutamate were returned to the control level by the administration of GTS in striatum, frontal cortex and hippocampus. After the infusion of AF64A, the level of taurine was decreased in striatum and increased in hippocampus. GTS administrations in the AF64A-treated rats restored to the control level of taurine in the decreased striatal level of taurine, but not in the elevated level of hippocampal taurine. The specific [$^3$H]MK801 binding sites in hippocampus was significantly decreased but not in striatum and frontal cortex after the administration of AF64A. Although GTS itself did not affect the specific [$^3$H]MK801 binding sites, GTS administrations in the AF64A-treated rats did decrease the binding sites of (\`H)Mk801 in all examined regions. The activities of striatal glutamine synthetase were decreased after GTS treatment. The activities of striatal glutamine synthetase (GS) were decreased in AF64A-treated groups. However, the decreased striatal GS activities by AF64A were returned to the control level by GTS treatment. Furthermore, GTS administrations in the AF64A-treated rats increased the hippocampal GS activities. The results indicatethat GTS may adjust the levels of glutamate and taurine constantly and may induce increase in AF64A-induced decrease of GS activity. Thus, it suggests that GTS may antagonize changes in central glutamatergic nervous system induced by AF64A. Also it suggests that the actions of GTS may differently affect in the disease state.

• Journal of Plant Biotechnology
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• v.32 no.4
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• pp.243-250
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• 2005

The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV.

• Journal of Veterinary Clinics
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• v.26 no.1
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• pp.36-40
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• 2009

The purpose of this study was to determine the incidence and therapeutic outcomes of canine oral tumors in Korea. Medical records of dogs presented with oral tumor at the Veterinary Medical Teaching Hospital of Seoul National University from 2002 to 2007 were reviewed. The predominant breeds were Yorkshire terrier (n=14), miniature poodle (n=7), Shih Tzu (n=6), and maltese (n=4), in order. The mean age at onset was $10.0{\pm}3.8$ years. The most common tumor types included fibrous epulis (n=13), malignant melanoma (n=12), acanthomatous epulis (n=4), and squamous cell carcinoma (n=3). Surgical removal was performed in 29 cases; simple excision in 18 cases, partial mandibulectomy in 8 cases, and partial maxillectomy in 3 dogs. Outcomes of surgery were various according to the tumor types. Prognosis of dogs with fibrous epulis and acanthomatous epulis was good and that of dogs with malignant melanoma was poor.