• Korean Journal of Acupuncture
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• v.27 no.1
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• pp.63-85
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• 2010

Objective & Method: The purpose of this study is to investigate the effects of herbal-acupuncture with Corni Fructus extract (CF-HA) at Eumgok(KI10) on osteoporosis in ovariectomized (OVX) ddy mice. I carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, the levels of serum ALP, osteocalcin, Ca, P and the levels of tibial Ca, P, Ca/P ratio, and we performed histological and histomorphological analysis as well. Result: 1. CF-HA at Eumgok(KI10) significantly did not increase the level of bone mineral density in overiectomized mice. 2. CF-HA at Eumgok(KI10) significantly decreased the level of serum phosphorus in ovariectomized mice and sinificantly increased the level of serum calcium in ovariectomized mice. 3. CF-HA at Eumgok(KI10) significantly increased the levels of tibial calcium and phosphorus in ovariectomized mice. 4. CF-HA at Eumgok(KI10) significantly decreased the number of tibial osteoclast like cell in ovariectomized mice. 5. In the histomorphometric analysis of tibia, GPL(growth plate length) was significantly decreased by CF-HA at Eumgok(KI10) in ovariectomized mice. Conclusions: These results suggest that CF-HA at Eumgok(KI10) may have useful therapy effects on osteoporosis in ovariectomized mice.

• Korean Journal of Acupuncture
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• v.26 no.1
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• pp.139-159
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• 2009

Objective & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with Lycii Fructus (LF-HA) at KI10 (Eumgok) on osteoporosis in ovariectomized (OVX) ddy mice. We carried out several experimental items which were known to be related with osteoporosis. Results : 1. Tibial BMD was significantly increased in the LF-HA group. 2. Tibial Ca level was significantly increased in the LF-HA group. 3. Osteoclast like cell in tibia was significantly decreased in the LF-HA group. 4. Histological change of tibia was improved, the trabecular bone thickness was significantly increased and the growth plate length was significantly decreased in the LF-HA group. Conclusion : These results suggest that LF-HA at KI10 has therapeutic effects on osteoporosis especially by way of suppressing osteoclast cells in ovariectomized ddy mice.

• Journal of Acupuncture Research
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• v.28 no.6
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• pp.117-137
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• 2011

Objectives & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with $Artemisiae$ $Vulgaris$ $Folium$ extract(AaH-HA) at $KI_{10}$($Eumgok$) on osteoporosis in ovariectomized(OVX) ddy mice. We carried out several experimental items to analyze the changes in body weight, urine, weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : 1. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction phosphorus level in serum in ovariectomized mice. 2. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction creatinine level in serum in ovariectomized mice. 3. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the increase of tibial osteoclast cells in ovariectomized mice. 4. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the reduction of TBV(trabecular bone volume) and TBT(trabecular bone thickness) in ovariectomized mice. 5. Herbal-acupuncture with AaH-HA at $KI_{10}$ significantly inhibited the overgrowth of tibial growth plate length(GPL) in ovariectomized mice. Conclusions : In conclusion, our study suggested that Herbal-acupuncturing with AaH-HA at $KI_{10}$ can be effective treatment for osteoporosis.

• Korean Journal of Acupuncture
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• v.25 no.1
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• pp.165-196
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• 2008

Objective : The purpose of this study is to observe the effects of herbal-acupuncture with Acanthopanacis cortex extract (AC-HA) at KI10 (Eumgok) on osteoporosis in ovariectomized (OVX) ddy mice. Methods : We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum creatinine, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results : Tibial BMD was significantly decreased by OVX, while significantly increased by AC-HA at KI10. Serum creatinine level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Serum osteocalcin level was significantly increased by OVX, while significantly decreased by AC-HA at KI10. Tibial TBV(trabecular bone volume) was significantly decreased by OVX, while significantly increased by AC-HA at KI10. AC-HA at KI10 significantly suppressed the OVX-induced increase of GPL(growth plate length) of tibia in mice. Conclusion : These results suggest that AC-HA at KI10 has a therapeutic effect on osteoporosis in OVX mice.

• Journal of Acupuncture Research
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• v.26 no.2
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• pp.31-40
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• 2009

Objectives: The purpose of this study is to investigate the effects of Daedon($LR_1$) Supplementation and Eumgok($KI_{10}$) Draining on changes of cerebral blood flow in normal rats. Methods : Regional cerebral blood flow(rCBF) and mean arterial blood pressure(MABP) in normal rats are observed, and those mechanisms were also investigated with pre-treatment of indomethacin (IDM) and methylene blue(MTB) each. Results : In this study, $LR_1$ supplementation and $KI_{10}$ draining elevated level of rCBF after 30 min, but MABP level was lowered at 30 min, then recovered toward normal level. Pre-treatment with indomethacin (IDM), an inhibitor of cyclooxygenase, inhibited increase of rCBF effectively, and pre-treatment with methylene blue(MTB), an inhibitor of guanylate cyclase, also inhibited increase of rCBF levels. On the other hand pre-treatment with IDM or MTB did not affect MABP levels. Conclusions : In conclusion, these results suggest that $LR_1$ supplementation and $KI_{10}$ draining can increase rCBF, and the mechanisms are thought to be related to both of cyclooxygenase and Guanylate cyclase pathways.

• Journal of Acupuncture Research
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• v.26 no.2
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• pp.1-14
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• 2009

Objectives & Methods : The purpose of this study is to observe the effects of herbal-acupuncture with Epimedii Herba extract (EH-HA) at $KI_{10}$ (Eumgok) on osteoporosis in ovariectomized (OVX) ddY mice. We carried out several experimental items to analyze the changes in body weight, uterine weight, uterus index, tibial length, the ash bone weight, tibial BMD, serum ALP, serum osteocalcin, serum Ca, and the levels of Ca, P, Ca/P ratio in tibia, and we performed histological and histomorphological analysis as well. Results: 1. The tibial length and ash bone weight were insignificant, while BMD was significantly increased by in EH-HA in comparison with control group. 2. Serum ALP and serum osteocalcin were decreased and serum Ca was increased by in EH-HA in comparison with control group, But all were insignificant. 3. Ca and P level in tibia were significantly increased in EH-HA in comparison with control group, while Ca/P ratio was insignificant. 4. Osteoclast like cell in tibia was significantly decreased in EH-HA in comparison with control group. 5. In the histological study in tibia, TBV was significantly increased, TBT was insignificantly increased and GPL was significantly decreased in EH-HA in comparison with control group. Conclusions: These results suggest that EH-HA at $KI_{10}$ has a therapeutic effect on osteoporosis in OVX mice.

• Korean Journal of Acupuncture
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• v.25 no.3
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• pp.97-115
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• 2008

Objective & Methods : This study aimed to evaluate the effects of Cistanches Herba herbal-acupuncture (CH-HA) at KI10 (Umgok) on osteoporosis induced by ovariectomy in mice. The results were as follows; Results : CH-HA at KI10 significantly inhibited the overgrowth of tibia, the elevation of serum osteocalcin level, the reduction of tibial Ca and P level, the increase of tibial osteoclast like cells, the reduction of tibial TBV (trabecular bone volume), the overgrowth of tibial GPL (growth plate length) in ovariectomized mice. NP at KI10 significantly inhibited the reduction of BMD (bone mineral density) in ovariectomized mice, but CH-HA did not. Conclusion : Taken together, CH-HA at KI10 has a protective or therapeutic effect for osteoporosis in ovariectomized mice. Therefore, it is suggested that CH-HA may be an useful therapeutics in clinical filed after further researches.

• Journal of Acupuncture Research
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• v.28 no.6
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• pp.69-83
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• 2011

Objectives : This study aimed to evaluate the effects of Malvae Semen herbal-acupuncture (MS-HA) at$KI_{10}$($Eumgok$) on nephritis induced by lipopolysaccharide (LPS) in rat. Methods : Rats with nephritis induced by LPS, were treated with Malvae Semen herbal-acupuncture(MS-HA) injection at $KI_{10}$. Two control groups, N.P group and Saline group, were treated with 26 gauge needle at $KI_{10}$, 3 times a week. In Saline group, normal saline was injected at $KI_{10}$. To evaluate the effects of Malvae Semen Herbal-acupuncture at $KI_{10}$ on nephritis in rats, WBC, Neutrophils in blood, BUN, Creatinine, TNF-a, CINC-1 in serum, urinal volume, creatinine, total protein in urine and renal TNF-a, renal tissue were analyzed. Results : MS-HA at $KI_{10}$ significantly reduced WBC and neutrophil in blood, TNF-${\alpha}$ in serum of LPS-stimulated rats. MS-HA at $KI_{10}$ significantly reduced total protein level in urine. MS-HA at $KI_{10}$ reduced accumulation of neutrophil in renal tissue of LPS-stimulated rats. Conclusions : Taken together, MS-HA at $KI_{10}$ has a therapeutic effect on nephritis in LPS-stimulated rat. Therefore, it is suggested that MS-HA at $KI_{10}$ may be an useful therapeutics for nephritis in clinical field.