• 한국환경농학회지
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• 제28권4호
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• pp.378-385
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• 2009

Arsenic (As) is known to be very toxic and carcinogenic to human beings. Arsenic contaminated soil was collected from a timber mill site at Busan Metropolitan City, Korea, where chromated copper arsenate (CCA) had been used to protect wood from rotting caused by insects and microbial agents. The soil was stabilized using both natural oyster shells (NOS) and calcinated oyster shells (POS). The calcination of natural oyster shells was accomplished at a high temperature in order to activate quicklime from calcite. Two different oyster shell particle sizes (-#10 mesh and -#20 mesh) and curing periods of up to 28 days were investigated. The stabilization effectiveness was evaluated based on the Korean Standard Test (KST) method (1N HCl extraction). The stabilization results showed that the POS treatment was more effective than the NOS treatment at immobilizing the As in the contaminated soils. A significant As reduction (96%) was attained upon a POS treatment at 20 wt% and passed the Korean warning standard of 20 mg/kg ('Na' area). However, an As reduction of only 47% (169 mg/kg) was achieved upon a NOS treatment at 20 wt%. The -#20 mesh oyster shells seem to perform better than the -#10 materials. The scanning electron microscopy (SEM)-energy dispersive X-ray spectroscopy (EDX) results showed that As immobilization was strongly associated with Ca and O in the presence of Al and Si.

• 경영과정보연구
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• 제33권3호
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• pp.93-104
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• 2014

본 연구는 탄소배출권거래제가 국내에서 시행되기 전에 EU ETS에서 거래되고 있는 탄소배출권 선물시장이 현물시장에 대해 가격발견기능이 존재하는 지에 대해 실증분석을 수행하였다. 동시에 서로 다른 거래소에서 거래되고 있는 선물시장과 현물시장 간의 정보교환이 효율적으로 이뤄지고 있는 지에 대해서도 알아보았다. 실증분석에 사용된 자료는 2009년 4월 1일부터 2012년 11월 30일까지 총 899개의 일일 자료이다. VECM의 오차수정계수를 이용하여 분석했을 때 탄소배출권 EUA 선물시장은 BlueNext 현물시장에 대해 가격발견기능이 존재하는 것으로 나타났다. 추가적인 검정에서도 GG와 Hasbrouck의 정보비율이 0.5보다 높은 값을 가지는 것으로 나타나서 EUA 선물시장은 현물시장에 대해 가격발견기능이 존재한다는 결론을 얻었다. 그리고 이러한 결과는 서로 다른 거래소에서 거래되더라도 탄소배출권과 관련된 정보 교환이 효율적으로 이뤄지고 있음을 알 수 있다.

• Journal of Korean Neurosurgical Society
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• 제60권4호
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• pp.417-423
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• 2017

Objective : Repair of sensorial nerve defect is an important issue on peripheric nerve surgery. The aim of the present study was to determine the effects of sensory-motor nerve bridging on the denervated dermatomal area, in rats with sensory nerve defects, using a neural cell adhesion molecule (NCAM). Methods : We compared the efficacy of end-to-side (ETS) coaptation of the tibial nerve for sural nerve defect repair, in 32 Sprague-Dawley rats. Rats were assigned to 1 of 4 groups : group A was the sham operated group, group B rats had sural nerves sectioned and buried in neighboring muscles, group C experienced nerve sectioning and end-to-end (ETE) anastomosis, and group D had sural nerves sectioned and ETS anastomosis was performed using atibial nerve bridge. Neurological evaluation included the skin pinch test and histological evaluation was performed by assessing NCAM expression in nerve terminals. Results : Rats in the denervated group yielded negative results for the skin pinch tests, while animals in the surgical intervention groups (group C and D) demonstrated positive results. As predicted, there were no positively stained skin specimens in the denervated group (group B); however, the surgery groups demonstrated significant staining. NCAM expression was also significantly higher in the surgery groups. However, the mean NCAM values were not significantly different between group C and group D. Conclusion : Previous research indicates that ETE nerve repair is the gold standard for peripheral nerve defect repair. However, ETS repair is an effective alternative method in cases of sensorial nerve defect when ETE repair is not possible.

• 한국수정란이식학회지
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• 제31권4호
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• pp.335-347
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• 2016

Multiple interferon tau (IFNT) genes exist in bovine. An antiluteolytic substance secreted by the bovine conceptus and primarily responsible for maternal recognition of pregnancy is bovine trophoblast protein 1 (bIFNT1), a new type I interferon tau (IFNT) genes. The objectives of this research were to investigate whether multiple, distinct gene encode bIFNT1 and other type I bIFNT gene in the bovine genome and to examine expression of bIFNT1 and other bIFNTc1 mRNAs during conceptus development. These transcrips could be regulated through caudal-related homeobox-2 (CDX2) and ETS2 and/or AP1 (JUN) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. The presence of mRNAs encoded by bIFNT1 and type I bIFNTc1 genes were examined quantitatively via reverse transcription-polymerase chain reaction (RT-PCR) analysis of total cellular RNA (tcRNA) extracted from on day 17, 20 and 22 bovine conceptuses. The expression level of bIFNT1 was higher on day 17 transcripts were gradually weakly detectable on day 20 and 22. However, the other bIFNTc1 gene examined transcripts was highly expressed on day 20 and transcripts were weakly detectable on day 17 and 22 bovine conceptuses. Furthermore, human choriocarcinoma JEG3 was co-transfected with an -1kb-bIFNT1/c1-Luc constructs and several transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1-Luc increased when this constructs were co-transfected with, ETS2, AP1(JUN), CREBBP and/or CDX2. Also, bIFNTc1 gene was had very effect on activity by alone ETS2, and AP1 (JUN) expression factors in choriocarcinoma JEG3 cell. However, bIFNT1 gene expression of the upstream region was not identified. We demonstrated that the activities of bIFN genes are regulated by differential, tissue-specific and developmental competence during pregnancy.

• Asian-Australasian Journal of Animal Sciences
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• 제26권5호
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• pp.638-645
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• 2013

Interferon-tau (IFNT) is thought to be the conceptus protein that signals maternal recognition of pregnancy in ruminants. We and others have observed that OCT4 expression persists in the trophectoderm of ruminants; thus, both CDX2 and OCT4 coexist during the early stages of conceptus development. The aim of this study was to examine the effect of CDX2 and OCT4 on IFNT gene transcription when evaluated with other transcription factors. Human choriocarcinoma JEG-3 cells were cotransfected with an ovine IFNT (-654-bp)-luciferase reporter (-654-IFNT-Luc) construct and several transcription factor expression plasmids. Cotransfection of the reporter construct with Cdx2, Ets2 and Jun increased transcription of -654-IFNT-Luc by about 12-fold compared with transfection of the construct alone. When cells were initially transfected with Oct4 (0 h) followed by transfection with Cdx2, Ets2 and/or Jun 24 h later, the expression of -654-IFNT-Luc was reduced to control levels. OCT4 also inhibited the stimulatory activity of CDX2 alone, but not when CDX2 was combined with JUN and/or ETS2. Thus, when combined with the other transcription factors, OCT4 exhibited little inhibitory activity towards CDX2. An inhibitor of the transcriptional coactivator CREB binding protein (CREBBP), 12S E1A, reduced CDX2/ETS2/JUN stimulated -654-IFNT-Luc expression by about 40%, indicating that the formation of an appropriate transcription factor complex is required for maximum expression. In conclusion, the presence of OCT4 may initially minimize IFNT expression; however, as elongation proceeds, the increasing expression of CDX2 and formation of the transcription complex leads to greatly increased IFNT expression, resulting in pregnancy establishment in ruminants.

• Journal of Astronomy and Space Sciences
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• 제14권2호
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• pp.275-285
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• 1997

1989년 2월에 일본의 우주과학연구소(ISAS: Institute of Space and Astronautical Science)에서 발사한 과학위성 EXOS-D의 전기장 측정기와 지자기 자오선 동경 190/210 선상에 위치하고 있는 17개의 지상자력계들로부터 1994년 11월 09일 18시 50분부터 약 20분에 걸쳐 Pi 2 파동이 검출되었다. 우리가 이용한 지상자력계는 지자기 경도는 동경 185.02에서 동경 269.36사이, 지자기 위도는 -37.09에서 65.67사이에, L값은 1.00에서 5.89사이에 위치하고 있다. 또한 같은 시각에 또 다른 위성 ETS-VI의 자기장 측정기의 자료와 지상의 Kakioka(지자기 동경 208.00, 지자기 위도 26.70), Hermanus(지자기 동경 82.97, 지자기 위도 -33.78) 지상자력계의 자료도 함께 사용하여 비교하여 보았다. Pi 2 파동의 주파수를 알아내기 위하여 FFT를 이용하였으며, L값이 2.35인 EXOS-D 위성과 지상자력계들에서는 주파수가 약 25mHz에서 최대 값을 보였으나, L값이 6.60인 ETS-VI위성에서는 같은 주파수가 검출되지 않았다. 또한 지상자력계 중에서 MUT 지상자력계의 자료를 기준으로 하여 위성들과 나머지 지상자력계들간의 상관관계, 위상 차를 조사하여, 발견된 Pi 2 파 동 현상이 플라즈마구 내에서 형성된 공동(cavity) mode에 의한 현상임을 알 수 있었다.

• Toxicological Research
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• 제23권1호
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• pp.39-46
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• 2007

The aim of the study was to see if there is any differences in urinary 1-hydroxypyrene glucuronide (1-OHPG) and 2-naphthol levels in children ($8{\sim}14$ years old) and their mothers ($30{\sim}46$ years old) living three cities in South Korea (Seoul, Incheon and Pohang) and three in China (Changchun, Datong and Kunming), where the levels of air pollution varies. The factors related with urinary biomarkers levels were also evaluated. The study subjects consisted of 118 Korean (60 children and 58 their mothers) and 120 Chinese (60 children and 60 their mothers). Urinary 1-OHPG was measured by synchronous fluorescence spectroscopy after immuno-affinity purification using monoclonal antibody 8E11 and urinary 2-naphthol concentrations were determined by HPLC with fluorescence detector. Information on recent consumption of diet containing high PAHs, environmental tobacco smoke (ETS), type of cooking and heating fuels, and other life-style characteristics were collected by self-administered questionnaire. The arithmetic mean of urinary 1-OHPG levels (n = 120, $mean{\pm}SD$, $6.77{\pm}7.96{\mu}mol/mol$ creatinine) in Chinese were 10 fold higher than those in Korean (n = 118, $0.62{\pm}0.61{\mu}mol/mol$ creatinine) (P < 0.01). Urinary 2-naphthol levels in Chinese (n = 119, $59.50{\pm}82.29{\mu}g/g$ creatinine) were significantly higher than those in Korean (n = 117, $25.09{\pm}46.56{\mu}g/g$ creatinine) (P < 0.01). Urinary 1-OHPG and 2-naphthol levels were significantly higher in children living the polluted cities in China (Datong and Chanchun, respectively). Multiple linear regression analysis indicated that living in factory area (vs. residential area) and use of coal stove as heating fuel were significant predictors for urinary 1-OHPG (overall model $R^2$= 0.46, n = 204). And ETS was predictor for urinary 2-naphthol levels in Korean ($R^2$ = 0.36, n = 46). These results indicated that urinary 1-OHPG and 2-naphthol levels were related with different ambient particulate air pollution, type of heating fuels and ETS.

• 대한환경공학회지
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• 제33권2호
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• pp.71-76
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• 2011

본 연구는 Pb와 Cu로 오염된 사격장 토양을 대상으로 폐자원을 가공하여 만든 안정화제를 이용하여 안정화 처리를 수행하였다. 안정화제는 폐굴껍질을 소성하여 생석회로 가공한 Calcined oyster shells (COS)와 폐소뼈를 가공한 Waste cow bones (WCB)로 오염토 함량대비 각각 5%와 3%를 사용하여, 28일간 습윤양생한 후 각종 용출시험법을 통해 효율성을 평가 하였다. 증류수 용출 및 SPLP에서는 뚜렷한 안정화 효율이 관찰되지 않았는데, 이는 중성 또는 약산성 환경에서는 중금속의 용출량 자체가 많지 않기 때문으로 판단된다. 반면, TCLP 및 0.1 N HCl을 이용한 용출실험에서는 안정화 처리를 한 토양의 경우 중금속의 용출량이 확연하게 감소한 것을 확인할 수 있었다. 특히, 0.1 N HCl 용출 결과에서 Pb 99.9%, Cu 83.9%의 매우 높은 용출량 저감효과를 보였고, 이는 비교적 강산성 용출환경에서는 중금속의 안정화 효과가 크게 나타남을 의미한다. 연속추출 결과를 통해 Pb와 Cu 간에 안정화 경향성의 차이는 Pb가 불용성 물질로의 변환과 안정화 효율이 더 탁월한 것으로 판단된다. 본 연구는 폐자원을 이용한 안정화제가 오염토양 처리에 활용 가능함을 증명하고 있고, 또한 폐기물로 취급되는 폐자원의 재사용 방안을 제시한다는 점에서도 큰 의미를 갖는다.

• Genomics & Informatics
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• 제8권4호
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• pp.185-193
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• 2010

Histone deacetylation and demethylation are epigenetic mechanisms implicated in cancer. Studies regarding the role of modulation of gene expression utilizing the histone deacetylase inhibitor scriptaid and the demethylating agent 5-azacytidine in HL-60 leukemia cells have been limited. We studied the possibility of recovering epigenetically silenced genes by scriptaid and 5-azacytidine in human leukemia cells by DNA microarray analysis. The first group was leukemia cells that were cultured with 5-azacytidine. The second group was cultured with scriptaid. The other group was cultured with both agents. Two hundred seventy newly developed genes were expressed after the combination of 5-azacytidine and scriptaid. Twenty-nine genes were unchanged after the combination treatment of 5-azacytidine and scriptaid. Among the 270 genes, 13 genes were differed significantly from the control. HPGD, CPA3, CEACAM6, LOC653907, ETS1, RAB37, PMP22, FST, FOXC1, and CCL2 were up-regulated, and IGLL3, IGLL1, and ASS1 were down-regulated. Eleven genes associated with oncogenesis were found among the differentially expressed genes: ETS1, ASCL2, BTG2, BTG1, SLAMF6, CDKN2D, RRAS, RET, GIPC1, MAGEB, and RGL4. We report the results of our leukemia cell microarray profiles after epigenetic combination therapy with the hope that they are the starting point of selectively targeted epigenetic therapy.

• 자원ㆍ환경경제연구
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• 제27권4호
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• pp.667-694
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• 2018

2015년 1월부터 시행된 시장기반 온실가스감축수단인 한국 탄소배출권시장의 가격결정체계와 2차 이행연도로의 진행과정에서 발생하는 학습효과에 대하여 고찰한다. 분석결과, 1차와 2차 이행연도 간에 차이점이 존재하는 것으로 추정되었다. 장내요인의 경우, 2차 이행연도에서는 1차 이행연도에서 추정되지 않았던 KCU와 KOC 가격 거래량 변수가 유의하게 추정되었다. 또한, 대내외 경제상황 변수의 경우, 1차 이행연도에서는 모든 변수들이 유의하지 않았으나, 2차 이행연도에서는 금리, 환율, 주가변수에서 통계적 유의성이 확보되었다. 이는, 1차에서 2차 이행연도로 진행하면서 시장운영자인 정부와 시장참여자인 기업들이 1차 이행연도에서의 경험과 지식을 바탕으로 2차 이행연도에서의 배출권 관련 의사결정을 보다 효율화하는 학습효과에 기인한다. 한편, 중점분석대상인 KAU15와 KAU16 가격에 대하여 공통적으로 유의미한 설명변수로는 각 배출권의 이행연도 이듬해 2월과 3월의 명세서 작성 및 제출에 대한 제도이항변수가 존재하였다.