• 한국식품조리과학회지
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• 제15권4호
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• pp.410-417
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• 1999

우리나라 야산에서 쉽게 구할 수 있는 구황 식물인 질경이를 메탄올과 여러 용매로 추출하여 식품 부패 미생물에 대한 항균력을 실험하고 그 항균활성 물질을 분석하여 다음과 같은 결과를 얻었다. 질경이의 메탄올 추출물은 2000$\mu\textrm{g}$/ml 농도에서 B. subtilis 및 V. parahaemolyticus의 증식을 100% 억제하였다. 그리고 질경이의 ethylacetate 분획물은 1000$\mu\textrm{g}$/disc 농도에서 5종의 모든 실험 균주에 대하여 8.5~11mm 크기의 clear zone을 형성하여 가장 높은 항균력을 보였다. 질경이의 ethylacetate 분획물을 silica gel column chromatography와 TLC로 분리하여 얻은 fraction을 5가지 실험 균주에 대하여 항균 실험을 한 결과 3rd 분획물 중 4번째 분획물이 250$\mu\textrm{g}$/disc 농도에서 모든 균주에 대하여 10 mm 이상의 clear zone을 나타내었다. 항균력을 보인 질경이의 ethylacetate 3rd fraction No. 4를 HPLC로 단일 분리하여 얻은 peak IV로 부터 $^1$H-NMR 및 GC-MSD로 동정한 결과 hexadecanoic acid로 동정되었다.

• Preventive Nutrition and Food Science
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• 제5권1호
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• pp.37-41
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• 2000

The protective effects of the ethylacetate fraction of persimmon leaves(PEF) against experimentally induced gastric mucosal damage and gastric ulcers were evaluated in ratss. In prophylatic study, 100 mg/kg ethylacetate fraction of persimmon leaves (PEFH) exhibited a total protection of 73.8% and 65.7% against HCl-ethanol and 0.2N NaOH-induced gastric mucosal membrane lesions, respectively, which was superior to cimetidine 50 mg/kg, a commonly used anti-ulcer drug. PEFH showed excellent anti-ulcer effects against pylorus ligation induced gastric ulcers, compared to the control group, however, 50 mg/kg ethylacetate fraction of persimmon leaves (PEFL) and PEFH did not affect ulcers induced by water immersion stress, and that is inferior to cimetidine 50 mg/kg. In conclusion, the results suggest that the ethylacetate fraction of persimmon leaves can be used both in prevention and treatment of experimentally induced gastric mucosal damage and ulcers.

• 한국식품영양과학회지
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• 제28권4호
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• pp.822-829
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• 1999

최근 식품 산업의 발달로 장기간 식품 보존을 위하여 합성 보존제의 사용이 크게 증가하고 있는 실정이다. 그러나, 인체에 대한 유해성이 문제로 대두되면서 이를 대체할 천연 보존제의 개발이 절실히 요고되고 있는 실정이다. 따라서 본 연구는 우리나라 야산에서 쉽게 구할 수 있는 구황 식물인 민들레를 메탄올과 여러 용매로 추출하여 식품부패 미생물에 대한 항균력을 실험하고 그 항균 활성 물질을 분석하여 다음과 같은 결론을 얻었다. 민들레의 메탄올 추출물은 $2000\mu\textrm{g}/ml$ 농도에서 s. aureus를 완전히 억제하였고, L. monocytogenes는 98.43% 억제하였다. 그리고 민들레의 ethylacetate 분획물은 $1000\mu\textrm{g}/disc$ 농도에서 5종의 모든 시험균주에 대하여 clear zone을 형성하여 가장 높은 항균력을 보였다. 민들레의 ethyl­-acetate 분획물을 silica gel column chromatography와 TLC를 통해 분리하여 얻은 fraction을 5가지 시험균주에 대한 항균 실험 결과 3번째 3rd fraction의 항균력이 가장 높아 $250\mu\textrm{g}/disc$ 농도에서 E. coli를 제외한 모든 균주에 대하여 14mm 이상의 clear zone을 나타내었다. 항균력을 보인 민들레의 ethylacetate 분획의 3번째 3rd fraction을 먼저 HPLC로 분리하여 얻은 peak II로부터 $^{l}H-NMR$ 및 GC-MS로 분석한 결과 benzoic acid로 동정 되었다.

• 한국식품조리과학회지
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• 제15권2호
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• pp.114-120
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• 1999

The objective of this study was to determine antioxidant effect of ether and ethylacetate fractions of 70% ethanol extract of some food (acid treated or not) on perilla oil. Each fraction of food extract was added to perilla oil and stored for 0,3,6.9,11 days at 60$^{\circ}C$. Then, the peroxide value (POV) of perilla oil samples were analyzed. Perilla oil contained ${\gamma}$-tocopherol 0.6800 $\mu\textrm{g}$/mg, ${\alpha}$-tocopherol 0.3189 $\mu\textrm{g}$/mg. $\delta$-tocopherol 0.0463 $\mu\textrm{g}$/mg, but it was easily oxidized due to high linolenic acid content. To increase yield of ether and ethylacetate fractions from each food extract, the 70% ethanol extract was treated with 0.2% H$_2$SO$_4$ and fractionized by ether and ethylacetate. Among ether and ethylacetate fractions of 70% ethanol extracts of some food, the yield of ethylacetate fraction of acid treated Pueraria thunbergiana extract was 5 times more than that of ethylacetate fraction untreated with acid. Perilla oil which added 100 ppm ethylacetate fraction of acid treated Pueraria thunbergianan extract showed low POV (44.8 meq/kg) compared to POV (80.0 meq/kg) of control.

• 대한한의학회지
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• 제21권3호
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• pp.174-185
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• 2000

Objectives : This study was carried out to evaluate the effects of five fractions on cell viability, cell cycle progression and apoptosis. Methods : This study employed MTT assay, Cell cycle analysis, Cpp32 protease assay, DNA fragmentation assay and Quantitative RT-PCR analysis. Results : In MTT assay, the butanol fraction of Injinsaryung-San has showed magnificent viability, while the $H_2O$ fraction and ethylacetate fraction also showed higher viability than the control group. The $H_2O$ fraction of Injinsaryung-San has showed magnificent viability, and butanol fraction and ethylacetate fraction of Injinsaryung-San with etoposide have also showed higher viability than the only etoposide group. Cell cycle analysis showed that each fraction of Injinsaryung-San had no significant effect on the cell cycle. DNA fragmentation assay showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction carried inhibitory effects on apoptosis induction. Cpp32 protease activity assay showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction decreased Cpp32 protease activity, with the butanol fraction displaying greater effects. Quantitative RT-PCR showed that the butanol fraction, $H_2O$ fraction and ethylacetate fraction suppressed Fas and Bax genes, the butanol fraction increased BcI-2 gene, however no effect on Cpp32. Conclusions : The data shows that the butanol fraction of Injinsaryung-San increases the hepatocyte viability and has the heptocelluar protective effect by the suppression of apoptosis through gene regulation.

• 한국식품영양과학회지
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• 제24권5호
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• pp.695-701
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• 1995

The ethanol extract of leaf mustard(Brassica juncea) exhibiting high antimicrobial activities was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The highest antimicrobial activity for the bacteria tested was found in the ethylacetate fraction, but a lesser extent in the butanol fraction. In contrast to antimicrobial activity for the bacteria, both ethylacetate and butanol fractions showed weak antimicrobial activity for yeasts. Unknown compound A in the ethylacetate fraction which exhibited a strong antimicrobial activity was isolated by silica gel column chromatography and HPLC, and exhibited 9 times more antimicrobial activity than the ethylacetate fraction.

• 한국식품영양과학회지
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• 제32권7호
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• pp.1059-1065
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• 2003

우리나라 야산에서 손쉽게 구할 수 있는 구황식물인 목단피를 건조시켜 분쇄한 후 methanol과 여러 용매로 추출하여 식품 부패 미생물의 증식 억제 효과를 검색하고 그 항균물질을 분석하여 다음과 같은 결과를 얻었다. 목단피의 methanol 추출물은 1500$\mu\textrm{g}$/mL 농도에서 B. subtilis, L. monocytogenes, V parahaemolyticus의 증식을 100% 억제하였으며 S. aureus, E. coli도 같은 농도에서 각각 36.31%와 67.81%의 억제 효과를 보였다. 목단피의 ethylacetate 분획 추출물은 낮은 농도인 500$\mu\textrm{g}$/disc의 농도에서 식품 부패 미생물 5종 모두에 대하여 clear zone을 형성하여 항균력을 나타내었다. 특히 L. monocytogenes, E. coli균에 대해서는 낮은 농도인 500$\mu\textrm{g}$/disc에서 각각 12mm, 11mm의 clear zone을 형성하였다. 목단피의 ethylacetate추출물을 silica gel colum chromatography(7cm${\times}$1.2 cm)한 후 thin layer chromatography(TLC)를 실시하여 항균 실험을 한 결과 B. subtilis의 경우 500 $\mu\textrm{g}$/disc 농도에서 2nd fra. 1이 21mm, V. parahaemolyticus는 250$\mu\textrm{g}$/disc 농도에서 14mm를 형성하여 목단피의 methanol 추출물로부터 각 용매별로 계통 분획하여 분리한 ethylacetate 분획으로부터 silica gel column chromatograpy와 TLC를 하여 항균성을 보인 2nd fra. 1의 성분을 알아보기 위하여 HPLC로 단일분리하여 얻은 peak Ⅸ에 대한 compound를 GC-MS로 분석한 결과 peak Ⅸ에서는 isobutyl isopentanoate가 있는 것으로 추정되었다.

• 한국식품영양과학회지
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• 제36권3호
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• pp.255-259
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• 2007

The solvent extracts of Oenothera laciniata, which were extracted by using several solvents with different polarities, were prepared for utility as a natural preservative. The O. laciniata extract by 80% ethanol was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. The antibacterial activities and cell growth inhibition were investigated on each strain with the different concentrations of O. laciniata extracts. Antibacterial activities were shown in ethanol, ethylacetate, and butanol fraction of O. laciniata. However n-hexane, dichloromethane and water fraction showed weak antibacterial activity against the tested microorganisms. Among the five fractions, ethylacetate fraction showed the highest antibacterial activities against microorganisms tested, such as Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Escherichia coli, Salmonella Enteritidis and Salmonella Typhimurium. The polyphenolic compounds widely occurring in the traditional medicine plants have been reported to possess high antibacterial activity. The polyphenolic compounds from ethanol, n-hexane, dichloromethane, ethylacetate, butanol, and water fraction were 63.96 mg/g, 8.49 mg/g, 28.11 mg/g, 172.64 mg/g, 114.56 mg/g, and 34.91 mg/g, respectively. There are some relationships between antibacterial activity and polyphenol content in natural plant. The ethylacetate fraction could be suitable for the development of a food preservative.

• Plant Resources
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• 제8권2호
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• pp.145-154
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• 2005

The antimicrobial effect of each fraction after fractionation of an ethanol extract of leaf mustard was examined in terms of nucleic acid, chloroform, ethylacetate, and butanol. The ethylacetate fraction, which showed the strongest level of antimicrobial effect among the different ethanol extract fractions of leaf mustard, was isolated and purified using silica gel column chromatography and HPLC, respectively, to obtain a single antimicrobial substance called KLM-1. The antimicrobial effect of this substance was 10 times higher than that of the ethylacetate fraction. A further study is on the way to confirm the structure of the antimicrobial substance KLM-1 through LC/Mass and NMR.

• KSBB Journal
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• 제26권2호
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• pp.165-171
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• 2011

Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 ${\mu}g/mL$), and flavonoid (140 ${\mu}g/mL$). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 ${\mu}g/{\mu}L$ and SOD-like activity was over 90% at 50 ${\mu}g/mL$ concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 ${\mu}g/mL$. As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.