• KSBB Journal
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• v.16 no.2
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• pp.220-223
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• 2001

To determine the inhibition of mushroom tyrosinase activity, fresh and dried mugwort, Artemisia princeps was extracted initially with water and ethyl alcohol, and subsequently with hexane, chloroform and ethyl acetate in that order. The highest yield was obtained from the ethyl acetate (15.2%) and hexane (15.5%) fraction of the ethanolic extract of fresh and dried mugwort, respectively. For all fractions tested, the inhibition of tyrosinase activity by fresh mugwort was higher than that of dried mugwort, and the inhibition ratio of tyrosinase activity was 98.9% in the chloroform fraction and 96.7% in the hexane fraction.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.6
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• pp.804-808
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• 2011

We studied the antioxidant activity of Suaedajaponica, found in Korean mud flats. A 70% ethanol extraction yielded 9.74%. The 70% ethanol extract was further fractionated with hexane, chloroform, ethyl acetate, butanol, and water. The water fraction resulted in the highest extraction yields, but the ethyl acetate fraction resulted in the highest total phenol content and also total flavonoid content. The antioxidative activity of the fractions was evaluated using several methods and compared to commercial antioxidants. The scavenging activities of the hydroxyl radical, hydrogen radical, hydrogen peroxide, and xanthine oxidase of the ethyl acetate fraction were equivalent to that of BHA. The scavenging activities of the hydroxyl radical, hydrogen peroxide, and xanthine oxidase of the ethanol extracts were approximately 80~90% of that of BHA.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.3
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• pp.419-424
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• 2014

Metabolite profiling of blueberry (cultivar "Spartan") was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications.

• Korean Journal of Medicinal Crop Science
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• v.23 no.1
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• pp.20-26
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• 2015

In the present study, we investigated biological activities of Rosa davurica Pall. leaves in order to evaluate the possibility as a natural biomaterial. The 80% ethanol extract and its subsequent fractions of Rosa davurica Pall. leaves were prepared using several solvents with different polarities. The extraction yield was 33.4, 36.6, 25.2, 18.7, 5.8 and 5.8% in ethanol extract, aqueous, butanol, ethyl acetate, n-hexane, chloroform fractions, respectively. The ethyl acetate fraction (661.38 mg/g), butanol fraction (396.68 mg/g) and 80% ethanolic extract (239.54 mg/g) has higher total polyphenol contents than other fractions. The antioxidant activity was detected in ethanolic extract, ethyl acetate and butanol fractions. The ethyl acetate fraction showed the highest levels of DPPH radical scavenging activity ($IC_{50}$, $4.77{\mu}g/m{\ell}$). Moreover, the ethyl acetate fraction significantly inhibited production of NO in LPS-stimulated macrophage RAW 264.7 cells without cytotoxicity. These results indicate that 80% ethanol extract and its fractions of Rosa davurica Pall. leaf, especially ethyl acetate fraction, have the properties of anti-oxidant and anti-inflammation, suggesting leaf of Rosa davurica Pall. may be a candidate for natural and functional materials.

• Food Science and Biotechnology
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• v.15 no.6
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• pp.889-895
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• 2006

Cytotoxic effects of extracts from Tremella fuciformis strain FB001 were evaluated on the DLD-1 human colon adenocarcinoma cell line and the content of polyphenolic compounds in the extracts were analyzed. Hexane, chloroform, and ethyl acetate subfractions (experimental setting I) exhibited cytotoxic effects on the human colon adenocarcinoma DLD-1 cell line with $IC_{50}$ values of 350, 400, and 450 ppm, respectively. When T. fuciformis was extracted sequentially with ether, ethyl acetate, chloroform, and ethanol (experimental setting II), the ether extract demonstrated potent cytotoxicity with an $IC_{50}$ value of 150 ppm, followed by ethyl acetate and chloroform fractions. If the first extraction solvent was chloroform instead of ether (experimental setting III), exposure of the cell line to chloroform, ethyl acetate, and ether extracts at 1,000 ppm led to cell death. High levels of phenolic compounds were estimated for all hydrophobic extracts, which exhibited cytotoxic effects. We propose that this useful information gives additional support to our understanding of the biology and utility of this particular mushroom.

• Korean Journal of Food Science and Technology
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• v.37 no.3
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• pp.474-478
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• 2005

Antioxidative effects of several solvents extracts of cactus Cheonnyuncho (Opuntia humifusa) grown in Korea were investigated. Because 70% ethanol extract showed relatively high antioxidative activity and extraction yield, it was sequentially fractionated with hexane, chloroform, ethyl acetate, butanol, and water, Ethyl acetate fraction showed highest scavenging activity against free radical DPPH. Antioxidative activity of ethyl acetate fraction determined based on acid and peroxide values under accelerated oxidation condition of lard was similar to that of ${\alpha}$-tocopherol, but slightly lower than that of BHA. Similar results were observed using TBA method during peroxidation of linoleic acid.

• Biomedical Science Letters
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• v.6 no.3
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• pp.181-186
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• 2000

Asadisulphide were purified from Ferrula assafoetida by organic solvent extraction and chromatography. Its inhibitory effects on the TPA-induced adherence of HL-60 cells was analyzed. Since ethyl acetate extracts of F. assafoetida has the strongest inhibitory effect on adherence of HL-60 cells, it was re-extracted with ethyl acetate, hexane, and ethyl ether and chromatographed three times to isolate asadisulphide. At the minimum concentration of 2 $\mu\textrm{g}$/ml, asadisulphide inhibited adherence of 98% of HL-60 cells that have been treated with TPA. It also showed anti-cancer effect with no cytotoxity in the ED$_{50}$ value of 20 $\mu\textrm{g}$/ml.

• Textile Coloration and Finishing
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• v.10 no.6
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• pp.27-32
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• 1998

도시 Traditionally, dyes extracted from onion shells have been used as natural pigments but dyeing methods with reproducibility have not been developed. In this study, the dyes were analyzed by high performance thin layer chromatography(HPTLC) to obtain the ratio of pigments in water and ethyl acetate extract. Furthermore, the present study was also carried out to obtain the information characteristic of wavelength which proportionally decrease for continuous dyeing reaction. Scanning of water extract showed 4 peaks by the mobile phase of benzene/ethyl acetate/acetic acid(40/10/5) and the peak 1 exhibited yellow color with the maximal absorption spectra of 306nm and 309nm while ethyl acetate extract showed 9 peaks in the same mobile phase. The water extract after ethyl acetate extraction was adjusted to 0.5 of O.D.(optical density) at 550nm by adding of water and compared wavelength of the pigments from 200 to 600nm to find the proportional decrease of wavelength. As the result, it showed that wavelength of 306nm could be the standard of dyeing monitoring points.

• Korean Journal of Medicinal Crop Science
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• v.6 no.2
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• pp.91-95
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• 1998

Some physical properties and chemical compositions of Lycii Cortex depending on the various extracting conditions were investigated for their changes during extraction with ethanol and water. Solid matter and contents of total sugar in water extracts were higher than those of ethanol extract. but contents of tannic acid in ethanol extracts was higher than that in water extracts. The methanol extract of the Lycii Cortex were fractionated with ethyl ether. ethyl acetate. n-butanol and water. The yield was higher in the order of water > n - butanol > ethyl ether and ethyl acetate fraction. Turbidity of water extraction was higher than that of ethanol extraction.

• The Korean Journal of Food And Nutrition
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• v.35 no.4
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• pp.276-281
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• 2022

Antioxidant activities and α-glucosidase inhibitory activities of Taraxacum officinale solvent fractions were measured. Extraction yields (relative to raw material) of 50% ethanol, hexane, ethyl acetate, butanol, and water were found to be 10.29, 2.61, 5.54, 2.15, and 0.96%, respectively. Polyphenol and flavonoid contents were high in ethyl acetate extract of Taraxacum officinale at 56.88 mg gallic acid/g and 33.27 mg gallic acid/g, respectively. DPPH, hydroxyl radical scavenging activity, and SOD-like activity measurement (IC₅₀%) of Taraxacum officinale 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions were 22.64, 18.65, 10.29, 20.81, 20.46 mg/mL, 24.68, 10.69, respectively. It was found to be 9.66, 15.81, 13.77 mg/mL, 32.84, 17.09, 12.73, 33.63, and 33.91 mg/mL, and was high in the ethyl acetate layer. Results showed that α-glucosidase inhibitory activities of Taraxacum officinale solvent fraction were 25.75, 15.93, 35.87, 15.96, and 2.88% for 50% ethanol extract, hexane, butanol, ethyl acetate, and water fractions, respectively.