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Controlling effect of several surfactants on barley powdery mildew caused by Erysiphae graminis (보리 흰가루병에 대한 몇 가지 계면활성제의 방제 효과)

  • Jang, Kyung-Soo;Yoo, Joo-Hyeon;Choi, Gyung-Ja;Kim, Jin-Cheol;Cho, Kwang-Yun;Kim, Heung-Tae
    • The Korean Journal of Pesticide Science
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    • v.5 no.2
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    • pp.51-57
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    • 2001
  • Controlling effect of several surfactants on barley powdery mildew (pathogen : Erysiphae graminis) and those influence on fungicidal activity by tank-mixing with commercialized fungicides were investigated in greenhouse test with barley seedlings. While protective activity of PNPP showed 90% at $1,000{\mu}g/mL$, that of LN 13.0 91.2%, tile best among 8 surfactants used in curative test. With $500{\mu}g/mL$, LN 13.0 showed 4% of disease percentage on the first leaf of narley continuously 4 days after the curative application. Excent for PNPP, the other surfactants, including NP 13.0, LN 13.0, and PAS, showed good inhibitory activity against spore germination in water agar medium amended with those surfactants. PAS, which showed very poor effect against barley powdery mildew in greenhouse test, strongly inhibited spore germination. Fungicide of triadimefon and triforine showed no controlling activity against Erysiphae graminis in narley seedlings, but they highly controlled powdery mildew in case of addition with $500{\mu}g/mL$ of LN 13.0 and Tween 20. SP 13.0, NP 13.0, LN 13.0 and DBC showed severe phytotoxicity in first leaf of barley seedlings, but Tween 20, SLIS, PAS, and PNPP not at $1,000{\mu}g/mL$.

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Free Radical Scavenging Activities and Inhibitory Effects on Xanthine Oxidase by Ethanol Extract from Capsella bursa-pastoris (냉이(Capsella bursa-pastoris)에탄을 추출물의 유리라디칼 소거 및 Xathine Oxidase 저해활성)

  • Hong, Jung-Il;Ra, Kyung-Soo;Sung, Ha-Chin;Yang, Han-Chul;Kweon, Mee-Hyang
    • Applied Biological Chemistry
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    • v.38 no.6
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    • pp.590-595
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    • 1995
  • To examine the characteristics of antioxidative compounds from Capsella bursa-pastoris, ethanol extracts were separated into five organic solvent fractions; hexane(Fr.H), diethyl ether (Fr.E), ethyl acetate(Fr.EA), butanol (Fr.B), and water(Fr.D) fractions. Fr.B showed the greatest electron donating ability and inhibitory effect on lipid peroxidation. Whereas Fr.E had the most excellent activity in the superoxide radical scavenging activity by xanthine/xanthine oxidase-cytochrome c reduction system. The inhibitory effect of each fraction on xanthine oxidase was also measured. Fr.E had the strongest inhibitory effect on xanthine oxidase and $IC_{50}$ was $5.65\;{\mu}g$. The results indicate that the superoxide radical scavenging activity of Fr.E is caused by the inhibitory effect on radical generating system of xanthine oxidase. Also the order of inhibitory effect on xanthine oxidase was Fr.B

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The Cytotoxic Effect of the Gleditsiae Semen Extracts on Human Colon Carcinoma Cells (조각인(Gleditsiae Semen) 추출물의 대장암 세포주에 대한 세포독성효과)

  • Cha, Mi-Ran;Yoon, Mi-Young;Kim, Ju-Young;Hwang, Ji-Hwan;Park, Hae-Ryong
    • Applied Biological Chemistry
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    • v.49 no.3
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    • pp.248-253
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    • 2006
  • The present study describes the preliminary evaluation of the cytotoxicity from Gleditsiae Semen extracts. G. Semen was extracted with methanol, ethanol, and acetone, and then cytotoxic effect of these extracts was measured by the MTT reduction assay and phase-contrast microscopy on the HT-29 human colon carcinoma cells. Among these extracts, methanol extract showed the highest cytotoxic activity on the HT-29 cells. The methanol extract was further fractionated with n-hexane, diethyl ether, ethyl acetate, and water layer according to the degree of polarity. The water layer showed the highest inhibitory activity on the growth of HT-29 cells, but the other fractions indicated the low cytotoxic activity. In addition, water layer also showed the cytotoxic activity against SW620 human colon carcinoma cells. Based on these results, we suggest that extracts of G. Semen may contain bioactive materials and are potential candidates as chemotherapeutic agents against human colon carcinoma cells.

The Study of KOGAS DME Process in Small and Medium Sized Gas Field Containing $CO_2$ ($CO_2$가 함유된 중소규모 가스전을 위한 KOGAS DME Process 연구)

  • Mo, Yong-Gi;Cho, Won-Jun;Song, Taek-Yong;Baek, Young-Soon
    • Journal of the Korean Institute of Gas
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    • v.14 no.4
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    • pp.51-55
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    • 2010
  • The global activities to reduce the $CO_2$ emission as a greenhouse gas have been various efforts. Under this circumstance, small and medium sized gas field containing $CO_2$ to develop as LNG is not economic feasibility. Particularly, for the separation of $CO_2$ in gas field, separation facilities should be installed to add. This is and increase in plant construction cost and separated $CO_2$ emission into the atmosphere is not the result of greenhouse gas reduction. When the uneconomic gas field apply the KOGAS DME process, the gas field containing $CO_2$ can be increase economic feasibility because of natural gas and $CO_2$ can be use to resource gas. The Tri-reformer produced syngas as H2 and CO in KOGAS DME process and the resource gases are natural gas, steam, oxygen and $CO_2$. The $CO_2$ is used as raw material gases from recover $CO_2$ in DME process. In this study, we investigated range of application of $CO_2$ in gas field.

A Study on the Utilization of Soy-Lecithin in Broiler Ration (Broiler에 있어서 대두 Lecithin의 사료적 가치에 관한 연구)

  • 김대진;김영길
    • Korean Journal of Poultry Science
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    • v.7 no.1
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    • pp.23-29
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    • 1980
  • The research was conducted to study the feeding value of crude soylecithin produced as by-products of soybean oil manufacturing in broiler ration. The results obtained were as follows. 1. The chemical composition, mineral composition, Vitamine contents and gross energy values of crude soy-lecithin were determined 2. No significant difference was observed for the body weight gain of broiler between control and lecithin groups of 3%, 6%, 9o/e and 12% addition. 3. Significant difference (P<0.05) was observed for feed intake lecithin 12% composed with lecithin 6% and 9% group, However, no significant difference was found among control, lecithin 3%, 6%, and 9% groups. 4. No significant difference was found for feed conversion between treatments. However, the lowest figure 2.32 for lecithin 9% treatments, followed by 2.40 of lecithin 6% and 2.41 of lecithin 3% orderly. 5. The composition of moisture, crude protein and ether extract in broiler carcas ranged from 71.2 to 72.15%, from 15.93 to 16.45% and from 6.19 to 7.05% respectively. 6. The weight of abdominal fat and live fat in broiler caress ranged from 42.4 to 63.7 gm, and from 2.2 to 2.4gm respectively. difference between abdominal and live fat weight was showing in significant statistically. 7. The present data reveal that soy-lecithin as by-product of soybean oil manufacturing was valuable energy source.

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Olefin Separation Membranes Based on PEO/PDMS-g-POEM Blends Containing AgBF4/Al(NO3)3 Mixed Salts (AgBF4/Al(NO3)3 혼합염이 포함된 PEO/PDMS-g-POEM 블렌드 기반의 올레핀 분리막)

  • Kim, Sang Jin;Jung, Jung Pyu;Park, Cheol Hun;Kim, Jong Hak
    • Membrane Journal
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    • v.25 no.6
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    • pp.496-502
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    • 2015
  • Facilitated transport is one of the possible solutions to simultaneously improve permeability and selectivity, which is challenging in conventional polymer-based membranes. Olefin/paraffin separation using facilitated transport membrane has received much attention as an alternative solution to the conventional distillation process. Herein, we report olefin separation composite membranes based on the polymer blends containing $AgBF_4/Al(NO_3)_3$ mixed salts. Free radical polymerization process was used to synthesize an amphiphilic graft copolymer of poly(dimethyl siloxane)-graft- poly(ethylene glycol) methyl ether methacrylate (PDMS-g-POEM). In addition, poly(ethylene oxide) (PEO) was introduced to the PDMS-g-POEM graft copolymer to form polymer blends with various ratios. The propylene/propane mixed-gas selectivity and permeance reached up to 5.6 and 10.05 GPU, respectively, when the PEO loading was 70 wt% in polymer blend. The improvement of olefin separation performance was attributed to the olefin facilitating silver ions as well as the highly permeable blend matrix. The stabilization of silver ions in the composite membrane was achieved through the introduction of $Al(NO_3)_3$ which suppressed the reduction of silver ions to silver particles.

Water Vapor Permeation Properties of Al2O3/TiO2 Passivation Layer Deposited by Atomic Layer Deposition (원자층 증착법을 이용한 Al2O3/TiO2 보호막의 수분 보호 특성)

  • Kwon, Tae-Suk;Moon, Yeon-Keon;Kim, Woong-Sun;Moon, Dae-Yong;Kim, Kyung-Taek;Shin, Sae-Young;Han, Dong-Suk;Park, Jae-Gun;Park, Jong-Wan
    • Journal of the Korean Vacuum Society
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    • v.19 no.6
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    • pp.495-500
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    • 2010
  • In this study, $Al_2O_3$ and $TiO_2$ films was deposited on to PES (poly(ethersulfon) substrate by using atomic layer deposition as functions of deposition temperature and plasma power. The density and carbon contents of $Al_2O_3$ and $TiO_2$ films was changed by varying process conditions. High density thin films was achieved through optimizing the process conditions. Buffer layer was deposited prior to the processing of upper thin films to avoid PES surface destruction during the high power plasma process and to enhances the tortuous path for water vapor permeation for the defect decoupling effect. The water vapor transmission rate by using MOCON test was investigated to analyze the effect. Water vaper permeation properties was improved by using the inorganic multi-layer passivation layer and activation energy of the water vapor permeation was increased.

Therapeutic Effect of Hovenia dulcis Thunberg Extracts and 6 Types Herbal Extracts on $CCl_4-induced$ Chronic Organ Damages in the Rats-Liver and Kidney (Carbon Tetrachlorid으로 유발된 Rat의 만성 장기(간, 신장) 손상시 헛개나무열매 추출물등 6종 생약의 투여시 조직병리학적변화)

  • Lim, Mee-Kyoung;Kim, Joo-Wan;Kim, Hong-Tae;Lee, Byoung-Je;Ku, Sae-Kwang;Kang, Mi-Young;Lee, Keun-Woo
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.384-391
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    • 2007
  • This study was conducted to clarify the effects of the Korean raisin (Hovenia dulcis Thunb) extracts and polyherbal extracts consisted of 6 types herbal extracts including HDT (HDTmix) on $CCl_4$ induced organ damages. Extracts were prepared by autoclave ($121^{\circ}C$, 15 psi, 3 hours) and filtered with nylon cloth and filter paper then freezing dried. Male Sprague-Dawley rats $(200^{\circ}{\ae}20g)$ were used as experimental groups, which were divided into 5 groups; intact control group (100 mg/kg), $CCl_4$ dosing group (100 mg/kg), $CCl_4$ dosing after HDT extract dosing group (100 mg/kg), $CCl_4$ dosing after HDTmix dosing group (100 mg/kg), Silymarin dosing group (5 mg/kg) after all test articles were orally dosed once a day for 28 days. They were sacrifiled under ether anesthesia. HDT extracts and HDTmix dramatically inhibits the $CCl_4$ intoxicated hepato/nephropathies with immuno-suppress changes on the spleen. They showed more dramatical protective effects on most of specific organs compared to that of Silymarin 5 mg/kg except for hepatoprotective effects in which, quite similar effects were detected. In addition, HDT extracts showed synergic effects with other types of herbal extracts because HDTmix showed more favorable protective effects on the all specific organs showing $CCl_4-related$ histopathological changes compared to HDT extracts.

Pretreatment Effect on the Analysis of Insoluble Hexavalent Chromium (전처리방법에 따른 불용성 6가 크롬 분석에 관한 연구)

  • Lee, Eun Jung;Roh, Jae Hoon;Won, Jong Uk;Chun, Mi Ryonng;Cho, Myung Hwa;Kim, Chi Nyon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.6 no.2
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    • pp.292-300
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    • 1996
  • Hexavalent chromium($Cr^{+6}$) compounds are considered to be particularly hazardous, primarily because of the associated risk of allergic reaction and cancer. The analytic method of hexavalent chromium such as the s-diphenylcarba-zide(DPC) method and all ether previously used methods are often made uncertain due to significant interferences from organic components. This report can provide a technique for the more rapid and simple determination of total hexavalent chromium. than other currently using methods. The s-diphenylcarbazide method proposed by the U.S. National Institute for Occupational Safety and Health has low recovery rate(15.67 - 48.20%) due to interference, iron chloride and nickel chloride. A microwave oven technique has high recovery rate(about 70%) of insoluble hexavalent chromium. For the difference of ionic charges of $Cr^{+3}$-ethylenediamine tetraacetic acid(EDTA) chelate and $CrO_4{^{-2}}$, we could detect them simultaneously by ion exchanged high performance liquid chromatography. The confirmation of $Cr^{+3}$ and $Cr^{+6}$ were checked by fraction collector and flameless atomic absorption spectrometer. We observed that the small amount of hexavalent chromium is converted to trivalent chromium due to enhancement of chromium reduction by $Fe^{+3}$ or $Ni^{+2}$. As a result of this study, on the analysis of insoluble hexavalent chromium with microwave oven was used for, it may be better and more precise analysis after pretreatment by 2% NaOH-3% $Na_2CO_3$ and then analysis UV-spectrophotometer. It should be done for various studies on insoluble hexavalent chromium on the basis work environmental monitoring so called welding, painting etc.

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Two endemic foci of heterophyids and other intestinal fluke infections in southern and western coastal areas in Korea (전북 부안군과 경남 사천군 해안지역에서 발견한 이형흡충류 및 기타 장흡충류의 유행지)

  • 채종일;송태의
    • Parasites, Hosts and Diseases
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    • v.36 no.3
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    • pp.155-162
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    • 1998
  • Two endemic foci of heterophyid infections were discovered in coastal villages of Puan-gun. Chollabuk-do, and Sachon-gun. Kyongsangnam-do, Korea. Fecal examina- tions were performed on 153 inhabitants of Puan-gun and 138 of Sachon-gun, using cellophane thick smear and formalin-ether sedimentation technique. The helminth egg and/or protozoan cyst positive rate was 21.5% (33/153) in Puan-gun and 39.1% (54/138) in Sachon-gun. In Puan-fun, the egg Positive rate of heterophyids was the highest, 17.6%, and that of other parasites was 0.7-2.6% by parasite species. In Sachon-gun, that of heterophyids wa's 18.8%, followed by Clonorchis sinensis (12.3%). and other parasites (0.7-5.OBEI . Twenth-two (Puan-gun) and six (Sachon-gun) heterophyid egg Positive cases were treated loth praziquantel, and adult flukes were collected from their diarrheic stools. A total of 3,284 adult flukes of Heterophyes nocens was collected from all of the 22 patients treated in Puan-gun (3-778 individually) , and other trematodes were also collected from 2- 15 patients; Ftgidiopsis sumac, Stellcntchcsmus JcEcctus, Metogoninus Wokogaupci, M. miyntci, Stictodora Juscnta, Heterophwopsis continua, Acnnthopcrwphium kurognmo, and GWmnophaELoines seoi. In Sachon-gun, M. yokognwci (3,007 specimens), H. nocens (120), aud S. falcatus (46) were collected from 5 of 6 treated patients, and H. continuo and 5. tan each from one patient. The present study revealed that heterophyid flukes, especially If. nocens and M. Wokofowai, are prevalent in the southern and western coastal areas of Korea where fresh and/or brackish water fishes are popularly eaten raw.

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