• 여성건강간호학회지
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• 제9권1호
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• pp.70-79
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• 2003

The purpose of this study was to investigate the effects of Aromatherapy, inhalhtion method using Clarysage essential oil on stress in middle-age women. The study design was a one-group pretest-posttest design. The subject were 44 middle-age women with stress in inhalation method of Clarysage essential oil was 2 drops of oil on tissue at 10, 14, 18, before bed for 2 weeks. The study was carried out from August 1 to September 20, 2002. The scale used in the study was the stress scale of Park, Sun Young(1999). The study data was analyzed by frepuency, percent age and t-test using SPSS Win 10.0 program. Result were as follows : 1. The score of physical stress scale was significantly decresed after use of inhalation of Clarysage essential oil. 2. The score of psychological stress scale was significantly decresed after use of inhalation of Clarysage essential oil. In conclusion, the results of this study suggest that inlalation method using Clarysage essential oil is positively supported in stress reduction in middle-aged women.

• 식물병연구
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• 제24권4호
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• pp.292-301
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• 2018

Alternaria leaf spot disease has been a concern during a tomato production in greenhouse. In vitro antifungal activities of vapours of four plant essential oils, cinnamon oil, fennel oil, origanum oil and thyme oil, were investigated during in vitro conidial germination and mycelial growth of Alternaria alternata causing the tomato leaf spots to find eco-friendly alternatives for chemical fungicides. The four plant essential oils showed different antifungal activities against in vitro conidial germination of A. alternata in dose-dependent manners, and cinnamon oil vapour was most effective to suppress the conidial germination. The four plant essential oils showed similar antifungal activities against the in vitro mycelial growth of A. alternata in dose-dependent manners, but low doses of thyme oil vapour slightly increased in vitro mycelial growth of A. alternata. Necrotic lesions on the A. alternata-inoculated tomato leaves were reduced differently depending on kinds and concentrations of plant essential oils. Delayed conidial germination and germ-tube elongation of A. alternata were found on the tomato leaves treated with cinnamon oil and origanum oil vapours at 6 hpi. These results suggest that volatiles from cinnamon oil and origanum oil can be provided as alternatives to manage Alternaria leaf spot during the tomato production eco-friendly.

• Natural Product Sciences
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• 제23권4호
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• pp.235-238
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• 2017

Colorado potato beetle is a most destructive insect pest of potato throughout the world. Although utilization of chemical insecticides is a main method for management of this pest, their negative side-effects such as threat to humans and the environmental pollution prompted researchers to search for natural alternatives. Recently plant essential oils with low or without side-effects against noun-targeted organisms and with high availability were considered as safe bio-pesticides. In the present study, toxicity of essential oil of Iranian lemongrass, Cymbopogon citratus Stapf, was evaluated against 3th instar larvae and adults of Colorado potato beetle by a leaf dipping method. Results displayed essential oil had notable toxicity against both larvae and adults after 24 and 48 h exposure times. Probit analysis revealed $LC_{50}$ values (lethal concentration to kill 50% of population) with 95% confidence limits were 10.32 (9.17 - 11.72) and 7.76 (6.80 - 8.74) ${\mu}l/ml$ for larvae and 6.27 (4.82 - 8.15) and 4.35 (3.24 - 5.62) ${\mu}l/ml$ for adults after 24 and 48 h, respectively. Based on regression analysis, a positive correlation between log concentration of essential oil and insect mortality was achieved. Results indicated C. citratus essential oil can be candidate as a natural alternative to the harmful chemical insecticides in the management of Colorado potato beetle.

• Food Quality and Culture
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• 제3권1호
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• pp.15-19
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• 2009

Gochujang samples with essential oils were evaluated for sensory including taste and, smell during various storage periods with the aim of developing improved high value-added products. The sensory testing was performed on Gochujang with 0.005% Zanthoxylum piperitum and Chrysanthemum indicum essential oils, extracted through steam distillation. During storage at 4 and $20^{\circ}C$ for 8 weeks, sensory testing was performed at 0, 1, 2, 4 and 8 weeks by trained panelists. Overall quality decreased in Gochujang samples containing Z.piperitum essential oil and increased in samples containing C.indicum essential oil, at 4 and $20^{\circ}C$. Samples stored at $4^{\circ}C$ were quality more then those stored at $20^{\circ}C$. Sensory evaluation was highest for Gochujang with C.indicum essential oil stored at $4^{\circ}C$. It is concluded that functional Gochujang can be developed by adding small quantities of essential oils. Increasing the amount of essential oil added is likely to affect the innate taste and flavor of Gochujang.

• 약학회지
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• 제54권2호
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• pp.122-125
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• 2010

The essential oil fraction was extracted from the aerial parts of the plant by steam distillation method and its composition was analyzed by GC-MS (gas chromatography-mass spectrometry) which led to the identification of 43 compounds. Dehydroelsholtzia ketone (56.81%) and elsholtzia ketone (30.05%) were identified as the predominant components of this oil. The antibacterial activities of the essential oil fraction were assessed by micro-dilution tests against antibioticsusceptible and -resistant strains of Streptococcus pneumoniae, Staphylococcus aureus, Salmonella enteritidis, and S. typhimurium. The oil inhibited most of the tested strains significantly resulting MICs (minimum inhibiting concentrations) between 2 mg/ml and >16 mg/ml. In most cases of this study Streptococcus pneumoniae and Staphylococcus aureus showed higher sensitivity to this oil than Salmonella strains.

• Natural Product Sciences
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• 제7권4호
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• pp.120-123
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• 2001

To develop systems for economic production of useful essential oil compounds, callus was induced from the seedlings of Agastache rugosa and cultured on MS medium. The volatile oil fraction was extracted from the callus and investigated by mean of GC-MS. The composition of the oil was compared with that of the mother plant. As a result, sixty five compounds including ferruginol were identified in the essential oil fraction. The main component of the oil from the leaves of Agastache rugosa was methyl chavichol (53.6%). Methyl jasmonate and jasmonic acid were added to the culturing cell suspension, separately and the composition of induced oil were compared. The oils from cultured cells treated with jasmonates showed considerably different patterns. Especially, the peak of estragole was found in callus oil after treatment with methyl jasmonate as though the amount was limited to 0.58%. In general, the TIC pattern of GC-MS of the callus oil became more similar to the oil from the leaves after elicitation.

• 한국연초학회지
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• 제25권1호
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• pp.7-11
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• 2003

Repellencies of herb plants and essential oils against alate Myzus persicae were investigated in a insect cage, a screen house, and a tobacco field. Numbers of nymphs deposited by alate or apterous Myzur persicae were lower on rosemary(Rosmarinus officinalis), silver thyme (Thymus vulgaris), and lemon balm(Melissa of fcianazis). Rosemary and silver thyme show little repellency against alate Myzus persicae on a nearby tobacco plant Rosemary oil, peppermint (Mentha piperita) oil, and ginger oil showed repellency against alate Myzus persicae, but spearmint (Mentha spicata) oil and lavender oil showed little repellency in a screen house. Rosemary oil, ginger oil, peppermint oil, lavender oil, and spearmint oil showed repellency against alate Myzus persicae in a tobacco field, but there was no significant difference among oils.

• Preventive Nutrition and Food Science
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• 제11권2호
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• pp.100-104
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• 2006

The in vitro antimicrobial and antioxidant activities of the essential oil and methanol extracts of Ledum palustre L. were investigated. Using GC-MS analysis, we identified 37 compounds in the essential oil, constituting 87.58% of the total oil. There are several monoterpenes, of which sabinene is the major compound ($16{\sim}17%$). There are several oxygenated monoterpenes of which terpinen-4-ol(7.6%) and myrtenal (3.5%) are the main constituents. $\beta$-Selinene, a-selinene, $\gamma$-elemene, a-caryophyllene are the main sesquiterpenes ($2{\sim}6%$ range). The oil strongly reduced the diphenylpicrylhydrazyl radical ($IC_{50}=1.56{\mu}g/mL$) formation and exhibited a hydroxyl radical scavenging effect in the $Fe^{3+}-EDTA-H_2O_2$ deoxyribose system ($IC_{50}=2.7{\mu}g/mL$), and also inhibited the nonenzymatic lipid peroxidation of rat liver homogenate ($IC_{50}=13.5{\mu}g/mL$). The polar phase of the extract showed antioxidant activity. The oil showed antimicrobial activity against Streptococcus pneumoniae, Clostridium perfringens, Candida albicans, Mycobacterium smegmatis, Acinetobacter lwoffii and Candida krusei while the water-insoluble parts of the methanolic extracts exhibited slight or no activity. This study confirms that the essential oil of Ledum palustre L. possesses antioxidant and low antimicrobial properties in vitro.

• Weed & Turfgrass Science
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• 제1권4호
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• pp.44-49
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• 2012

본 연구의 목적은 아미리스 정유의 제초활성을 검정하는데 있다. 아미리스 정유의 기내 종자발아 제초활성 검정 결과 유채에 대한 $GR_{50}$값은 8.8 ${\mu}g\;g^{-1}$ 이었으며, 온실 제초활성 검정 결과 바랭이에 대한 완전고사 경엽처리 약량은 1,250 ${\mu}g\;ml^{-1}$ 이었다. 화본과잡초 4종과 광엽잡초 5종을 대상으로한 온실실험에서 4,000 ${\mu}g\;ml^{-1}$ 약량처리시 아미리스 정유는 수수, 돌피, 자귀풀에 대해 각각 90%, 70% 그리고 70%의 약효를 나타내었으며, 아미리스 정유에 처리된 잎은 모두 건조되는 증상을 나타내었다. 포장시험에서 아미리스 정유 5%의 피, 냉이, 토끼풀에 대한 제초효과는 모두 70%이었으며, 10%에서는 100%이었다. 아미리스 10% 정유의 제초활성은 처리후 6시간부터 발현되기 시작하였으며, 아미리스 정유 처리구 잡초의 지상부위 생체중은 대조구와 비교하여 41.5%이었으나 지하부위 생체중은 대조구와 비교하여 차이가 없었다. 아미리스 정유에는 총 15종의 화학물질이 검출되었으며 주된 화합물은 calarene(27.24%), elemol(19.38%), ${\gamma}$-eudesmol(9.24%), curcumene(5.85%), ${\beta}$-sesquiphellandrene(5.63%), zingiberene(5.50%), selina-3,7(11)-diene(5.29), 1,3-diisopropenyl-6-methyl-cyclohexene(4.18), ${\beta}$-bisabolene(4.13%), ${\beta}$-maaliene(3.62%)이었다. 본 연구의 결과 아미리스 정유는 속효성, 비선택적, 비이행성 제초특성을 갖는 것으로 추론된다.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.62-70
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• 2014

The principle purpose of this research is to figure out whether the inhalation of Wood Essential oil (Chamaecyparis obtusa) affect not only human beings' concentration for surroundings but also change as to status of feelings. total number of subjects for this experimental are determined to be 66 (men 44 and women 22) among 85 adults who agreed to participate in this experiment after excluding 19 participants who were not able to smell scent due to flu and nasal inflammation. After both experimental group and control group being measured referring to their status of mood such as concentration, depression, vigor, fatigue, confusion, tension and anger, the experimental group is offered to get a piece of cotton that includes Wood Essential oil and they are asked to inhale it for 5 min. On the other hand, control group is offered to wait for the experimental group finishing inhaling wood Essential oil for 5 min. After 5 min, the status of mood from both groups were measured again. simultaneously, I conducted a survey targeting experimental group to see what their preferences are like regarding scent of wood essential oil. The experiment shows that the status of experimental group's concentration is improved about 0.94 point when compared to control group's. Moreover, Total mood disturbance is lower than control group's. Therefore it is proved that the inhalation of wood Essential oil alleviates overall status of mood. according to the analyses result of favor to scent for wood essential oil from experimental group, some of them who were positive got much better improvement when it comes to concentration and far more relieved on their feelings than those who weren't. Based from this result, it is definitely confirmed that there is a Phytoncide's psychophysiological effect which transcends the Placebo Effect generated by Phytoncide's positive image.