• Korean Journal of Ichthyology
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• v.29 no.3
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• pp.157-164
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• 2017

In 2015, a nervous necrosis virus (NNV) was isolated from sevenband grouper, Epinephelus septemfasciatus, maintained in land-based aquaculture system at below $12^{\circ}C$ in winter. Mortality was up to 30% in brood fish, over 4 kg of body weight. Moribund fish showed clinical sings typical of viral encephalopathy and retinopathy (VER), also called viral nervous necrosis (VNN), such as uncoordinated, corkscrew-like swimming behavior, belly-up at rest, darkening of body, cloudy eyeball and hyperinflation of the swim bladder. Aetiology of the disease was confirmed by gross observation of clinical signs, histopathology and molecular diagnosis. Histological studies revealed severe vacuolation and necrosis in the brain. Molecular diagnosis by revere transcription-polymerase chain reaction (RT-PCR) specific to batanodavirus yielded a positive result. The nucleotide sequences of the PCR-amplified fragment were 99.48~100% similar to barfin flounder nervous necrosis virus (BFNNV) genotype and most closely aligned with Pacific cod betanodavirus (PCNNV). This is the first report of natural batanodavirus, NNV infection in sevenband grouper reared in low water temperature during winter (below $12^{\circ}C$) in Korea.

• Journal of fish pathology
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• v.11 no.2
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• pp.97-104
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• 1998

Since 1989, mass mortality has repeatly occurred in cage-cultured sevenband grouper, Epinephelus septemfasciatus along the southern coast of Korea in the summer season and usually reached over 80% within a few months. Diseased fish showed the clinical signs of anorexia, dark coloration, loss of eqilibrium, spinal swimming behaviour, vertebral deformity and inflation of swim bladder. Histopathologically, necrosis and/or vacuolation of the nerve cells in the brain and retina were observed. We previously reported that the causative agent was filtrable. The causative agent was not culturable in various fish cells; RTG-2, CHSE-214, BF-2, EPC and FHM. However, electron microscopic observation revealed unenveloped icosahedral viral particles with about 30 nm in diameter in the cytoplasm of nerve cells of the brain. The characteristics of the virus was tested by an artificial infection with the filtrate of the homogenate of diseased fish. The pathogenicity of the virus was retained after treatment with ether or heat ($50^{\circ}C$, 30 min) but partly lost by pH 3 or 11 treatment. These results suggest that the causative agent are similar to the fish nodavirus. In order to compare the causative agent with a fish nodavirus, Striped Jack Nervous Necrosis Virus (SJNNV), a polymerase chain reaction (PCR) was performed with primers specific to SJNNV. As a result, about 430 by PCR products were detected from the brain and the eye of both naturally and artificially infected sevenband grouper. All these results represent that the mass mortality in the cultured sevenband grouper is caused by the infection of a nodavirus similar to SJNNV and this is the first report of a fish nodavirus from the cultured sevenband grouper in Korea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.715-722
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• 2002

The monthly collected fish samples by crab pots were analyzed in order to study the species composition and seasonal variation of fish assemblage in the coastal water off Gadeok-do, Korea in 1998, During the study period, 49 species of fishes representing 26 families were collected, The dominant species were Conger myriaster, Sebastes inermis, Hexagrammos otakii, H. agrammus, Stephanolepis cirrhifer and Acanthogobius flavimanus. These 6 dominant species accounted for $67.1\%$ and $69.4\%$ in the number of individuals and biomass, respectively. The secondary importance species were Thamnaconus modestus, Takifugu niphobles, Rudarius ercodes, Dictyosoma burgeri, Sebastes longispinis and Epinephelus septemfasciatus. The highest number of species was collected in November, and the number of individuals and biomass were highest in March. But the lowest numbers of species and individuals were collected in July and the lowest biomass in September, Although the species diversity indices varied little monthly, the lowest value was recorded in August because of small number of species and predominance of 5. cirrhifer and T. modestus.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.24 no.4
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• pp.158-163
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• 1988

The author has examined how sea-bass, Epinephelus septemfasciatus respond to surface attracting lamps line and underwater attracting lamps line in the experimental water tank (550L$\times$58W$\times$73H cm). The attracting rate was investigated in accordance with the intervals of lighting and putting out hour (1, 5 minute) when each of the attracting lamps was gradually switched off after they were switched on all at once. The results are as follows: 1. Distribution of fish at the illuminated section; (1). The fish was more distributed in the section adjacent to the extinguished section. (2). Difference of distribution between section and section and section was greater at surface attracting lamps as compared with underwater attracting lamps. (3). Surface attracting lamps was more distributed than underwater attracting lamps at intervals of 5 minutes. 2. Attracting rate of the last section; (1). The rate was showed increasing as illuminating time elapse and became L type. (2). Attracting rate in underwater attracting lamps was higher than surface attracting lamps at intervals of 1 minutes and lower than surface attracting lamps at intervals of 5 minuets.

• Journal of Marine Life Science
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• v.2 no.1
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• pp.1-6
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• 2017

The purpose of this research is to develop the simple freezing method for sperm cryopreservation in the Sevenband grouper and Kelp grouper. The motility and survival rate were checked to investigate the effects of diluent and cryoprotectant on sperm cryopreservation.In cryopreservation experiments, 300 mM glucose and 15% dimethyl sulfoxide (DMSO) were used as a diluent and a cryoprotectant, respectively. As a results of cryopreservation, the motility rate of Sevenband grouper was found to be more than 90%, and that of Kelp grouper was found to be more that 60% in sperm 5 months after cryopreservation.

• Korean Journal of Ichthyology
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• v.13 no.2
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• pp.143-157
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• 2001

To determine seasonal fluctuations in abundance and species composition of the fishes, samples were collected by bottom trawl off Kohung Penisula from February to December 1999. A total of 7,197 fishes were sampled and identified into 123 species, 54 families, 13 orders and 2 classes. Of the 13 orders, Perciformes, Scorpaniformes, Pleuronectiformes and Tetraodontiformes accounted for approximately 82.9% of the total. Gobiid fishes were dominant, representing 13 species. Leiognathus nuchalis, Konosirus punctatus, Engraulis japonicus, Ilisha elongata, Trichiurus japonicus, Argyrosomus argentatus, Ditrema temmincki, Takifugu niphobles, Hexagrammos otakii and Lateolabrax japonicus accounted for 71.4% of the number of individuals. Mugil cephalus, Sebastes schlegeli, Lateolabrax japonicus, Konosirus punctatus, Hexagrammos otakii, Conger myriaster, Liparis tanakai, Seriola quinqueradiata, Trichiurus japonicus, Sebastiscus marmoratus and Limanda yokohamae accounted for 50.7% of the total biomass. The number of species showed a seasonal variation, higher in spring, summer and autumn than in winter. The Largest numbers of individuals and greater biomass were observed in August. The economic fishes of this area were Konosirus punctatus, Engraulis japonicus, Mugil cephalus, Sebastes inermis, Sebastes schlegeli, Sebastes oblongus, Sebastiscus marmoratus, Platycephalus indicus, Hexagrammos agrammus, Hexagrammos otakii, Lateolabrax japonicus, Epinephelus akaara, Epinephelus septemfasciatus, Seriola quinqueradiata, Pagrus major, Acanthopagrus schlegeli, Argyrosomus argentatus, Oplegnathus fasciatus, Trichiurus japonicus, Pampus echinogaster, Paralichthys olivaceus, Kareius bicoloratus, Limanda yokohamae and Takifugu porphyreus.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.4
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• pp.151-156
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• 1987

The author carried out an experiment to find out the response of sea-bass, Epinephelus septemfasciatus (Thunberg) to the color lights. The experimental tank (360L$\times$50W$\times$55H cm) was set up in a dark room. Six longitudinal sections with 60 cm intervals are marked in the tank to observe the location of the fish. Water depth in the tank was kept 50 cm level. Light bulbs of 20 W at the both ends of the tank projected the light horizontally into the tank. Two different colored filters were selected from four colors of red, blue, yellow, and white, and they were placed in front of the light bulbs to make different colors of light. Light intensity were controlled by use of auxiliary filters intercepted between the bulb and the filter. The fishes were acclimatized in the dark for 50 minutes before they were employed in the experiment. Upon turning on the light, the number of fish in each section was counted 40 times in 30 second intervals, and the mean of the number of fish in each section was given as the gathering rate of the fish. The colors favourited by the fish was found in the order of blue, red, white and yellow in day time, and red, blue, yellow and white at night time. The gathering rate of fish on illumination period was not constant and fluctuated with irregularity. The difference of the gathering rate on two different colors of light was small and the difference was larger in night time then in day time.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.3
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• pp.27-27
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• 1987

The purpose of this study is to find the light intensity which induced maximum gathering rate and to observe the variation of the gathering rate both at daytime and night by using Sea-bass, Epinephelus septemfasciatus (Thunberg). An experimental tank (360 L×50 W×55H cm) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Eight artificial light sources were prepared by combination of three with white light bulbs (10W, 60W, 100W) and eight filters. During the experiment water depth was maintained 50cm level in the tank. The tank was marked into six longitudinal sections each being 60cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes before the main experiment. Upon turning on the light, the number of fish in each section was counted 60 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 24.13 lux (15.25~35.93 lux) at daytime and 0.41 lux (0.25~0.63 lux) at night. The variation of the gathering rate of fish in illumination time was great and did not show any definite pattern but fluctuated irregularly. Its different between daytime and night is remarkable.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.23 no.3
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• pp.137-143
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• 1987

The purpose of this study is to find the light intensity which induced maximum gathering rate and to observe the variation of the gathering rate both at daytime and night by using Sea-bass, Epinephelus septemfasciatus (Thunberg). An experimental tank (360 L$\times$50 W$\times$55H cm) was set up in a dark room. An illumination system was attached to the end of one side of the tank to control horizontal light intensity. Eight artificial light sources were prepared by combination of three with white light bulbs (10W, 60W, 100W) and eight filters. During the experiment water depth was maintained 50cm level in the tank. The tank was marked into six longitudinal sections each being 60cm long to observe the distribution of fish. The fish were acclimatized in dark condition for 50 minutes before the main experiment. Upon turning on the light, the number of fish in each section was counted 60 times every 30 seconds, and the gathering rate was obtained from the average number of fish in each section. The light intensity inducing maximum gathering rate was 24.13 lux (15.25~35.93 lux) at daytime and 0.41 lux (0.25~0.63 lux) at night. The variation of the gathering rate of fish in illumination time was great and did not show any definite pattern but fluctuated irregularly. Its different between daytime and night is remarkable.

• Development and Reproduction
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• v.20 no.2
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• pp.51-63
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• 2016

The landmark-based morphometric and meristic analysis of the kelp grouper (Epinephelus bruneus), red spotted grouper (E. akaara) and seven-banded grouper (E. septemfasciatus) were performed to compare the differentiation of overall body shape and structure. The measurements of the morphometric dimensions were observed in 25 parts (truss dimension: 16 parts; head part dimension: 9 parts) of 38 morphometric dimensions and also meristic differences observed in 3 parts (dorsal fin, anal fin and caudal fin) of 6 meristic counts (P < 0.05). Observed morphometric characteristics primarily involved in truss and head part dimension, kelp grouper have larger values in caudal part of truss dimension, kelp grouper, red spotted grouper and seven-banded grouper have similar values in pectoral part of truss dimension, in addition to, results of head part dimension showed that red spotted grouper have smaller values in overall dimensions (P < 0.05). As meristic characteristics, kelp grouper have more number of anal fin rays than other fish, red spotted grouper have more number of dorsal soft rays than other fish, and seven spotted grouper have more number of anal soft rays, and caudal fin rays than other fish (P < 0.05). Photographed under the x-ray, kelp grouper have the most curved vertebral column and largest swim bladder than other fishes (P < 0.05). Our results of this study confirmed that 3 subfamily fishes adequately can distinguish with external body shape, and we hope that the results of our study could be used to identify in Serranidae family as taxonomical parameters.