• 한국식생활문화학회지
• /
• 제32권2호
• /
• pp.144-149
• /
• 2017

This study was designed to investigate the contents of icariin during ripening of Epimedium koreanum with sugar and Soju (25, 30, and 35% alcohol contents) using a homemade method. Contents of icariin of Epimedium wine were examined using high performance liquid chromatography. Icariin levels in Epimedium wine increased during the first 3 days of storage; after 6 days, icariin contents decreased gradually until 30 days. Contents of icariin in Epimedium wine by storage temperature (5 and $20^{\circ}C$) were not different during storage. After 30 days, icaiin was not detected in all tested Epimedium wines. Epimedium wines were assessed for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and (ABTS) radical scavenging activity. DPPH and ABTS radical scavenging activities increased in early storage periods, however, after 6 or 9 days, activities decreased gradually.

• Plant Resources
• /
• 제5권3호
• /
• pp.210-213
• /
• 2002

This study was performed to investigate the effect of Epimedium brevicornum Herba extract on the treatment of chromaticity and colorfastness. Epimedium brevicornum Herba has been used as a Korean medicine. It is effective in sexual excitement, depressant of blood pressure and anti-virus. It is also good as a tonic and in treating involuntary emission of semen. In the long history of Korea, dyeing has been applied for a means representing the grace of natural and inner esthetic consciousness of man. Vegetable dyes give us such great benefits, diversified color, but no pollution. And ramie fabric has distinctive features such as beautiful brilliance, elegance, and strong durability. So, it is regarded as a special product of Korea traditionally. These studies were carried out to treat with acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was died with Epimedium brevicornum Herba extract. The results of experiment showed as follows: First, the chromophoric degree was the highest in acetate iron but not distinction in another mordants. Second, the light colorfastness was the highest in non treated and dichloride copper, but alum was the lowest. Third, the discoloration was alum and dichloride copper showed first grade in washing colorfastness. Abrasion colorfastness was not significant in this test. According the previous results, Epimedium brevicornum Herba has an efficiency in sexual excitement, depressant of blood pressure and anti-virus. So it is considered that Epimedium brevicornum Herba can be applied effectively to tonic and in treating involuntary emission of semen.

• 대한한방부인과학회지
• /
• 제19권3호
• /
• pp.55-68
• /
• 2006

Purpose : This study was undertaken to evaluate the effects of the different administration duration of Epimedium Herb extract solution on the spermatogenic abilities such as concentration, motility and morphological normality of sperm from the testis and the activities of sperm hyaluronidase. Methods : We used the 2-month-old mice and administered the extract solution of Epimedium Herb 0.3 ml/g/day for 30, 60, 90 and 120 days. The control group was administered the normal saline as the same way. We examined the number of total, motile and normal sperm from the cauda epididymis, the activities of sperm hyaluronidase. Also we observed changes of isolated testis before and after administration of Epimedium Herb extract solutions in the mice. And we compared the testicular tissue especially seminiferous tubules with the control and treated group by histochemical methods. Results : The significant differences were observed in the concentration of total sperm and normality of spermatozoa of the Epimedium Herb extract solution administered groups compared to the control group in 60, 90 and 120 days groups. The significant differences were observed the motility of the Epimedium Herb extract solution administered groups compared to the control group in 60 days group. In the histological analysis of the testicular tissues, the enlargement of testicular lobe diameter and apparent vasculogenesis between testicular lobes were observed in the Epimedium Herb extract solution administered groups compared to the control group, respectively. And the activity of hyaluronidase was significantly increased in the Epimedium Herb extract solution administered groups compared to the control group. Conclusion : This study shows that Epimedium Herb has the beneficial effect on the concentration, morphology and motility of sperm, the testicular tissues and the activities of sperm hyaluronidse in 60 days administration group. We can suggest that Epimedium Herb extract solution be useful for the treatment of male sexual dysfunction and infertility.

• 대한한의학방제학회지
• /
• 제28권1호
• /
• pp.81-90
• /
• 2020

Objectives : This study was conducted to investigate the effects of major constituents of Epimedium koreanum Nakai (Icariin, epimedium A, epimedium B, and epimedium C) on melanin synthesis. Methods : We measured melanin contents, tyrosinase activity, and expression of Rab27a in B16F10 cells cultured with Epimedium koreanum Nakai ethanol extract (EKN) and their major constituents. After treatment with H89 and dibutyryl cAMP, which inhibit or promote the activation of PKA, we observed changes in melanin synthesis and tyrosinase activity stimulated by EKN. Results : Among them, EKN and icariin enhanced tyrosinase activity and melanin contents. We confirmed that EKN augmented melanin synthesis via cAMP/PKA pathway. Icariin-induced tyrosinase activity and melanin content were attenuated by PKA inhibitor H89, while melanogenic effect of icariin was further augmented by cAMP analog, dbc AMP. However, icariin did not affect the expression of small GTPase Rab27a involved in melanosome transport. Conclusions : These results suggest that icariin promotes melanogenesis through cAMP/PKA pathway but does not affect small GTPase Rab27a.

• Journal of Microbiology and Biotechnology
• /
• 제32권4호
• /
• pp.437-446
• /
• 2022

In this study, to obtain icaritin with high pharmacological activities from icariin, which has a content ratio of over 58% in the total flavonoids of Epimedium herb, a special Epimedium flavonoid-glycosidase was produced, purified and characterized from Aspergillus sp.y848 strain. The optimal enzyme production was gained in a medium containing 5% (w/v) wheat bran extract and 0.7% (w/v) Epimedium leaf powder as the enzyme inducer, and strain culture at 30℃ for 6-7 days. The molecular weight of the enzyme was approximately 73.2 kDa; the optimal pH and temperature were 5.0 and 40℃. The enzyme Km and Vmax values for icariin were 15.63 mM and 55.56 mM/h. Moreover, the enzyme hydrolyzed the 7-O-glucosides of icariin into icariside II, and finally hydrolyzed 3-Orhamnoside of icariside II into icaritin. The enzyme also hydrolyzed 7-O-glucosides of epimedin B to sagittatoside B, and then further hydrolyzed terminal 3-O-xyloside of sagittatoside B to icarisiede II, before finally hydrolyzing 3-O-rhamnoside of icarisiede II into icaritin. The enzyme only hydrolyzed 7-O-glucoside of epimedin A or epimedin C into sagittatoside A or sagittatoside C. It is possible to prepare icaritin from the high-content icariin in Epimedium herb using this enzyme. When 2.5% icariin was reacted at 40℃ for 18-20 h by the low-cost crude enzyme, 5.04 g icaritin with 98% purity was obtained from 10 g icariin. Also, the icaritin molar yield was 92.5%. Our results showed icaritin was successfully produced via cost-effective and relatively simple methods from icariin by crude enzyme. Our results should be very useful for the development of medicines from Epimedium herb.

• Bulletin of the Korean Chemical Society
• /
• 제22권10호
• /
• pp.1159-1162
• /
• 2001

• 생약학회지
• /
• 제19권2호
• /
• pp.93-96
• /
• 1988

Three flavonoids were isolated from Epimedium koreanum and identified as quercetin(1), $anhydroicaritin-3-O-{\alpha}-rhamnoside(2)$ and icariin(3) by spectroscopic methods. The former two compounds are the first isolation from this plant.

• Plant Resources
• /
• 제6권3호
• /
• pp.221-226
• /
• 2003

To improve industrial scale extraction method for extraction of icariin from Epimedium koreanum Nakai, the yields under different extracting conditions such as solvent, temperature, duration and solvent to plant material weight ratio were compared. Regarding extracting solution, highest extracts and icariin yield could be achieved when 10% EtOH was used. In case of plant material to extracting solvent ratio, no significant differences could be observed from 1/10 to 1/50, indicating 1/10 was the most efficient. Extracting temperature significantly affected extracts and icariin yields in that 9$0^{\circ}C$ increased the collected extracts and icariin contents up to 29.6% and 0.76%, respectively, compared to 27.2%, 0.33% at 7$0^{\circ}C$. The yield of extracts was less dependent upon extracting temperature compared to icariin yield. Regarding extraction time, 4 hr and 6 hr resulted in high extracts and icariin yield, respectively. We found extracting Epimedium koreanum Nakai in 10 times volume of 10% EtOH for 4 and 6 hr at 9$0^{\circ}C$ seem to be relatively efficient methods for extracts and icariin, respectively.

• 한국식품과학회지
• /
• 제24권6호
• /
• pp.535-540
• /
• 1992

음양곽(Epimedium koreanum N.)의 methanol 추출물을 각종 식용유지에 첨가하여 $75^{\circ}C$에서 저장하면서 과산화물가의 변화를 측정하므로서 항산화성을 검토하였던 바 유지의 종류에 따라 효력에 차이를 나타내었고 lard와 corn oil에 대한 항산화 효과가 인정되었으며, 특히 lard에 대하여는 항산화 효과가 켰으며 첨가농도에 따라 효과가 증대되었다. 음양곽 추출물을 $180^{\circ}C$에서 120분간 가열하였을 때에도 66% 이상의 항산화성이 잔존하였으나 대두유에 첨가하여 광산화에 미치는 영향을 검토한 결과 자외선 조사에 의하여 급격히 산화가 촉진되었다. 음양곽 methanol 추출물 중의 유효 항산화 성분은 tocopherol류와 flavonoid류로 추정되었으며 ${\alpha}$, ${\beta}$, ${\gamma}$ and ${\delta}-tocopherol$의 함량은 각각 21.61, 1.48, 5.50 및 3.75 mg%이었다.

• 한국자원식물학회지
• /
• 제10권4호
• /
• pp.422-427
• /
• 1997

유용자원식물의 자생지 재배기술의 확립을 위한 기초자료를 얻고저 참취와 삼지구엽초의 자생지 식생 및 환경(생태 및 토양화학성분) 조사를 실시한 결과는 다음과 같다. 1. 참취자생지인 춘천 A. 연엽산 A 그리고 연엽산 B에서의 종수는 20, 26, 25종이었고 삼지구엽초 자생지인 춘천 B, 북방면 그리고 대룡산에서는 15, 32, 26종이었다. 참취 자생지의 우점종은 춘천 A, 연엽산 A, 연엽산 B에서 맑은대쑥, 애기나리, 실새풀 등이었으며 삼지구엽초의 자생지인 춘천 B, 북방면, 대룡산에서는 고사리, 맑은대쑥, 애기나리 등이 우점하였다. 2. 자생지의 토양산도는 4.5-5.3 범위였으며 유기물 함량은 1.59(춘천A)$\sim$5.5(연엽산 B)이었다. 암모니아태 질소 및 질산태 질소함량은 참취 자생지와 삼지구엽초 자생지 토양에서 평균 각각 0.0043mg/kg, 0.0025mg/kg이었다. 3. 자생지 토양의 $NH_4-N$의 성분은 참취의 자생지의 토양성분이 삼지구엽초의 자생지 토양성분보다 약간 높은 수치를 보였다. 그밖의 성분의 차이는 크게 나타나지 않았다.