• Animal cells and systems
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• v.12 no.2
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• pp.85-91
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• 2008

Structure and mucosubstance histochemistry in the epidermis of five teleostean species, i. e., spotty belly, Agrammus agrammus, devil stinger, Inimicus japonicus, stone fish, Erosa erosa, cubed snailfish, Liparis tessellatus, and Japanese bluefish, Scombrops boops were investigated. The epidermis of five species studied is composed of three layers: superficial, middle, and basal layer. The superficial layer is comprised of rather flattened cells. Mucous cells, the type commonly found in fishes are completely lacking in the epidermis of devil stinger, stone fish, and cubed snailfish. The epidermis of devil stinger and stone fish have multicellular glands which do not have mucosubstances. The skin surface of them is covered with mucous layer. The superficial cells in the epidermis of devil stinger, stone fish, and cubed snailfish are mucus-secreting cells. The composition and the amount of the mucosubstances vary in species and body regions. The mucous layer on the skin surface and superficial epidermal cells of devil stinger contain a mixture of neutral and acidic(sulfated and nonsulfated) mucosubstances. In stone fish, the mucous layer has acidic(sulfated and non-sulfated) mucin and the superficial epidermal cells contain neutral mucin. In cubed snailfish, the type of epidermal mucosubstances is identified as a mixture of neutral and acidic(non-sulfated) mucin. The mucous cells of the epidermis in spotty belly and Japanese bluefish contain neutral mucin.

• Animal cells and systems
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• v.4 no.4
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• pp.315-318
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• 2000

Structure of the skin in amphibious fish, Periophthalmus modestus, was described in relation to cutaneous respiration. The epidermis has no gland cell. The epidermis consists of three regions: outermost layer of one to five layers of flattened epithelial cells, middle layer of swollen epithelial cells instead of glandular cells and stratum germinativum of cuboidal cells. There are numerous blood capillaries in the outermost layer of the epidermis and diffusion distance between the blood of capillaries and the epidermis is about 1.4 Um. The middle layer of the epidermis appears to be a web-like structure due to the swollen epithelial cells. The stratum germinativum has a well-developed lymphatic space containing lymphocytes. There are numerous blood capillaries and elliptical area with acid mucopolysaccharides in stratum laxum of the dermis. The skin of Periophthalmus modestus may be an accessory respiratory organ for oxygen uptake during terrestrial or aquatic life.

• Applied Microscopy
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• v.52
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• pp.13.1-13.6
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• 2022

Microscopic structure of skin in two amphibious mudskipper fish; Boleophthalmus dussumieri Valenciennes, 1837 (B. dussumeri) and Periophthalmus waltoni Koumans, 1941 (P. waltoni) were investigated in relation to their lifestyle. The general structure of skin is the same among the two species. Epidermis in B. dussumeri was thicker significantly than P. waltoni. The dermal bulges were only well developed in the skin of B. dussumeri. Mucous cells were absent in the epidermis of P. waltoni but present in B. dussumeri. Both B. dussumeri and P. waltoni have well-developed swollen middle cells as a shared epidermal feature. The thickness of the middle cell layer of the epidermis in B. dussumeri was significantly greater than in P. waltoni. Capillaries in the dorsal and ventral parts of the body are more closely distributed to the epidermal surface in P. waltoni than in B. dussumeri. The diffusion distance in the dorsal epidermis of P. waltoni was less than that in the ventral epidermis of B. dussumeri. A comparative examination of the skin of mudskipper species suggests that, due to the more terrestrial lifestyle adopted by P. waltoni, the skin contributes more to respiration.

• Animal cells and systems
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• v.11 no.2
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• pp.155-160
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• 2007

The skin of the Korean bullheads, Pseudobagrus brevicorpus and P. koreanus, is composed of epidermis, dermis and subcutis. The epidermis has three layers, the outermost layer, middle layer and stratum germinativum. The epidermis consists of two types of gland cells, an unicellular mucous cell of sulfomucin and a large club cell having sometimes two nuclei. The epidermis has numerous intraepithelial blood vessels in P. brevicorpus but not at all in P. koreanus. Lymphatic spaces containing lymphocytes are well developed in mainly the stratum germinativum. The dermis lacks scales and consists mostly of bundles of coarse collagen fibers. The collagen bundles are arranged in parallel to each other in the dorsum and lateral region toward the dorsum, but vertically at intervals in the abdomen and lateral region toward the abdomen. Considering this unique skin structure, the two species are likely to exercise cutaneous respiration as a dual respiratory system to overcome hypoxic conditions which frequently occurs in their habitats.

• Journal of Pharmaceutical Investigation
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• v.19 no.1
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• pp.39-46
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• 1989

The clonidine transport mechanism through the skin was investigated with assumptions that epidermis is heterogeneous and two-layer membrane. Immobilization of clonidine was not found in stratum corneum but in viable epidermis. The sorption in the viable epidermis agreed with the dual sorption theory. Diffusion coefficient in stratum corneum was five order magnitude less than that in viable epidermis. In viable epidermis, the ratio of true diffusivity to apparent diffusivity increased initially then decreased as a function of clonidine concentration, and the true diffusivity was always larger than the apparent diffusivity.

• Korean Journal of Ichthyology
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• v.17 no.1
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• pp.36-42
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• 2005

Histological observations on the barbels of catfish (Siluridae) in Korea are reported. The mandibular barbel of Far Eastern catfish, Silurus asotus, has epidermis and dermis surrounding an axial rod of cartilage. The stratified epidermis embodies mucous cells, club cells, and taste buds. The mucous cells, which are thin rims of basophilic cytoplasm, and the taste buds, which are basophilic, are located at the surface and the distal portion of the epidermis, respectively. The basement membrane lies between the epidermis and dermis except in places of dermal papillae. The dermis is made up of loose connective tissue fibers enclosing nerve bundles and blood vessels. The central region of cartilage is innermost and enclosed within muscle layers. The mandibular barbel of slender catfish, S. microdorsalis, has an identical structure to those of the Far Eastern catfish however, the percentage thickness of epidermis in the upper and lower parts of the barbel and the number of taste buds in the upper and middle parts of the barbel are different from those of Far Eastern catfish. This study shows that the mandibular barbels in Far Eastern catfish and slender catfish are of a stiff and flexible type.

• Animal cells and systems
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• v.8 no.2
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• pp.111-115
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• 2004

The epidermis of the mudskipper, Periophthalmus modestus, consists of three layers- the outermost layer, middle layer and stratum germinativum. Extensive fine blood capillaries are present near the superficial layer of epidermis and outermost layer in five fins and a sucking disc. The diffusion distance between the vascular capillaries and the surface of epidermis ranged from 3.6 to 10.9${\mu}$m: 3.6 ${\mu}$m in the sucking disc, 10.9 ${\mu}$m in the anal fin and 4.6 to 5.0 ${\mu}$m in the two dorsal fins. Rate of the surface area of respiratory epithelium, the surface area of the fine blood capillaries occupied per surface area of epidermis in 0.1mm, is 3.7 to 4.4% in two dorsal fins and 1.1% in the anal fin. The middle layer is simpler in structure consisting of small or voluminous cells swollen by epidermal cells, and this layer appeared web-like. Well-developed lymphatic spaces containing lymphocytes existed in the stratum germinativum. The five fins and sucking disc had no epidermal glands.

• Applied Microscopy
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• v.35 no.2
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• pp.97-104
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• 2005

Plant epidermis consists of relatively unspecialized cells and more specialized cells of various structure and function. Trichomes are specialized cells originated from the epidermis and much attention has been paid to the plants developing trichomes with peculiar structure and function. The present study has been undertaken to examine the trichome type noticed in the leaf epidermis of Althaea rosea using scanning electron microscopy. Four types, namely simple, short-and long-tufted, and glandular hairs, were detected in their epidermis. Their Distribution, frequency and structure varied by the development and epidermal surface. The most frequently distinguished type was the tufted ones growing in young leaves of the abaxial epidermis, while the simple hairs were rare throught the examination. The short-tufted hairs branched up to seven times having each branchlet about $160{\sim}210{\mu}m$ in length at maturity. The long-tufted hairs exhibited up to ten branchlets, where branchlets could reach up to $900{\sim}1,000{\mu}m$ long when fully expanded. Glandular trichome was the peltate type comprising $1{\sim}2$ secretory head cells, 2 stalk cells and a basal cell. The short peltate glandular hairs, usually not exceeding $40{\mu}m$, differentiated more along the areoles in the adaxial epidermis. The function of these trichomes in A. rosea has been still obscure, but it has been speculated that they probably play a role in protection; non-glandular ones possibly providing a defense against insects and secretory glandular type participating in chemical defense. Structural features of these trichomes at cellular level will be discussed in the following study of transmission electron microscopy.

• Horticultural Science & Technology
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• v.18 no.3
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• pp.368-372
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• 2000

The fruit structure of 'Fuji' apples from full bloom to maturing was observed from 1997 to 1998. Cell division period of the fruit was found to be 4 to 5 weeks after full bloom. Vascular bundles in the inner part of the fruit skin which were not described in the books illustrating apple fruit structure was observed, as they were tentatively named as outer vascular bundles (OVB), and another vascular bundles were also observed newly in periphery of locules, as they were tentatively named as inner vascular bundles (IVB). In the observation of the inner epidermis (IE) in the inner part of the locules on 2 days prior to full bloom, the guard cells were observed and these were disappeared in the observation made 2 days later, i.e. on full bloom. The formation of fruit skin was observed at the microscope 65 days after full bloom and the number of cell which organized the fruit skin did not change from this time to maturation period. Tannin which is mainly in the fruit skin changing from continuously during fruit growth, specially the tannin of epidermis disappeared completely 100 days after full bloom stage, and then constituted again. Starch was not almost found out in cell division period of fruit from full bloom stage after this time it constituted much at flesh part and decreased at maturation period. Epidermis was developed by uniform cells of a layer the cell of epidermis constituted irregularity after pigmentation stage, the organization of fruit was not close because the very big intercellular space constituted at hypodermis and flesh structure.

• Korean Journal of Ichthyology
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• v.23 no.3
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• pp.250-254
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• 2011

Modified organs for air respiration in Korean fish was reviewed in the following 6 Korean fishes: three mudskippers (Boleophthalmus pectinirostris, Periophthalmus modestus and P. magnuspinnatus), two mud loaches (Misgurnus mizolepis and M. anguillicaudatus), and a torrent catfish (Liobagrus mediadiposalis). Three mudskippers and a torrent catfish have a modified epidermis to in order to make up for the deficient oxygen supply. Their epidermis has abundant intraepithelial blood capillaries except dermal capillaries situated just beneath the stratum germinativum of the epidermis in B. pectinirostris. The epidermis was thick due to component of the following cells: two kinds of glands as a small mucous cells and a large club cells in L. mediadiposalis, voluminous cells (swollen cells) swollen by epidermal cells and a small mucous cells in B. pectinirostris, and only voluminous cells having no any glandular cells in P. modestus and P. magnuspinnatus. In Particular, the epidermis of the mudskippers appears to be a web-like structure due to the swollen epithelial cells. The dermal bulges are found in B. pectinirostris and they are situated at the skin covering the body, not appendage of all the fins and the sucking disc. Another modified organ in M. mizolepis and M. anguillicaudatus occurs in intestine and its mucosal epithelium has abundant blood capillaries.