• 한국염색가공학회지
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• 제13권4호
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• pp.227-233
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• 2001

Wool fabrics were pre-treated with corona prior to treating with enzyme for shrinkage resistance. Commercial protease and cellulase were used for degradation of wool and the treatment conditions such as enzyme amount, treating time, and assistant chemicals. Friction coefficient and zeta-potential were measured to certificate an effect of treatment condition on the handle of wool fabric. Corona pretreatment make the wool fabric soft, which result in the decrease of friction coefficient and zeta-potential. Scale removal of wool surface was observed by scanning electron microscope. Amino acid analysis shows the effectiveness of enzyme, and corona pretreatment does not cause severe internal damage.

• 한국미생물·생명공학회지
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• 제4권4호
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• pp.131-137
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• 1976

산업상 이용에 적합한 분리 선정된 Naringinase 생산 균주인 Asrpergillus niger S-1은 동시에 Hesperidinase를 강력히 생산함이 확인되었으며 이 균주가 생산하는 Hesperidinase의 효소학적 성질을 요약하면 다음과 같다. 1. PH 4.0에서 30분간 반응 시켰을때 최적 온도는 6$0^{\circ}C$ 이었으며 최적 작용 pH는 5.0이었으나 pH 3.0에서는 70% 활성을 보였다. 2. pH 4.0에서 30분간 열처리 하였을 경우 6$0^{\circ}C$로 열처리한 결과 90%, 7$0^{\circ}C$일 경우 70%, 8$0^{\circ}C$일 경우 65%의 활성을 가지는 비교적 내열성 효소이었다. 그리고 pH에 대한 안정성은 4$^{\circ}C$에서 24시간 보존한 결과 pH 5.0일 경우 가장 안정 하였다. 3. $Mg^{＋＋}$ 이온은 1$\times$$10^{-2}$M 이하부터는 활성화하였으나 Cu$^{＋＋}$ Mn$^{＋＋}$ Pb$^{＋＋}$ Mo$^{＋＋}$ Ag$^{＋＋}$ Hg$^{＋＋}$등은 저go 하였다. 4. 효소를 Aceton 처리할 경우 60% Aceton 처리에서 배양 추출물의 경우 보다 11배 전제되었으며 35%가 회수 되었으며 40%의 Aceton 처리일 경우 10배 정제되었으며 5.6%가 회수 되었다. 5. 배양 추출 효소를 유안으로 염석할 경우 0.4.~0.6 분획 침전 시킬 경우 48배 정제되었으며 처음 효소의 13%가 회수되었고 0.6~0.8포화 분획에서도 19%가 회수되었고 25배 정제되었다. 6. Hesperidinase 생성은 약간량의 밀감 과피 추출물의 첨가로 현저히 증가 하였다.

• 한국염색가공학회지
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• 제5권3호
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• pp.206-215
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• 1993

In the prior study, wool gabardines were treated with alkaline proteases which were some kinds of enzyme to decompose protein, and their tensile strengths were determined, and the surface of the fibers were also observed using a scanning electron microscope. Enzylon ASN 30 and Alkalase 2.5L DX did not show much effect on the weight loss of wool, however, the weight loss of wool increased considerably with treating Esperase 8.0L. Pretreatment of wool with dichloloisocyanuric acid before protease-treatment increased the weight loss of wool to a great extent. In this study, the enzyme treated wools dyeing behaviors with acid dye, Milling Cyanine 5R, were mainly investigated. The protease-treatment remarkably increased not only the rate of dyeing but also the saturation dye uptake. From these results, it seemed likely that the structural relaxation of adhesive filler of interscale or intercellular cement facilitated the dye penetration into the fibers, at the same time, the change in the inner structure of the wool fibers by the protease made the fixation of the dyes more efficient.

• 한국염색가공학회지
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• 제14권1호
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• pp.18-26
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• 2002

Chitosan has reactive amino and hydroxyl groups which can be used to chemically alter its properties under the mild reaction conditions. Thus the cationization of Tencel with Chitosan is effective to modify the fabric. To investigate the modified properties of Tencel fabric, the tests were performed under the several finishing process with enzyme/glutaraldehyde/softener. The internal structure of Tencel which has the structure of cellulose II wasn't changed by enzyme, chitosan and crosslinking agent treatment and the thermal stability was improved by chitosan and crosslinking agent treatment. Wrinkle recovery angle under the dry condition increased highly until $0.1\textrm{mol}/\ell$ of glutaraldehyde concentration, and then decreased. Tensile strength of modified Tencel fabric decreased with increasing of weight loss, but it was improved more or less by chitosan, crosslinking agent and softener. Moisture regain was improved by enzyme and chitosan treatment. And antibacterial activity showed nearly 100% on Tencel fabric treated with 0.5% chitosan and adsorption of metal ion increased with increasing of chitosan concentration.

• 한국식품과학회지
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• 제43권4호
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• pp.407-412
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• 2011

본 연구에서는 녹차와 녹차에 미생물을 접종하여 발효시킨 후 발효차의 유리형 및 결합형 향기성분을 headspace-solid phase microextraction(HS-SPME)-GC/GC-MS를 이용하여 분석하였다. 녹차가 미생물학적 발효과정을 거치는 중에 녹차에 존재하는 aldehyde류와 ketone류는 감소하였고 linalool과 geraniol은 증가하였다. 녹차와 후발효차 잎에 추가적으로 효소를 처리하여 분석한 결과 녹차에서는 (Z)-3-hexen-1-ol>benzaldehyde>3-hexenyl acetate>geraniol순으로 향기성분이 증가되었고, 후발효차에서는 (Z)-3-hexen-1-ol>benzaldehyde>geraniol>3-hexenyl acetate>1-hexanol 순으로 증가하였으며, 녹차보다는 후발효차에서 효소에 의해 증가되는 향기성분이 많았다. 효소양을 달리하여 처리하였을 때 녹차와 후발효차 모두 효소의 양이 증가함에 따라 휘발성 향기성분이 증가하는 양상을 보였다. 본 실험을 통하여 효소처리를 통해 녹차와 후발효차의 향기를 증가시킴으로써 녹차와 후발효차의 향기 품질을 향상시킬 수 있는 가능성을 확인할 수 있었다.

• KSBB Journal
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• 제23권6호
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• pp.504-508
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• 2008

국내산 라이밀을 이용한 바이오에탄올 생산을 위해 저온 전처리 공정을 도입하여 에탄올 생산성을 비교하였다. 라이밀의 경우 원료 특성상 증자 공정에서 점도 문제가 발생하는데, 이를 해결하기 위해 최적 전처리 조건을 탐색하였으며 이에 따른 에탄올 생산성을 비교하였다. 저온 조건과 점도 저하 효소를 사용함으로서 점도에 따른 발효 저해 현상 해결하였고 전처리 공정에 소요되는 전처리 공정비를 절감할 수 있었다. 또한 pH 조절(pH 4.5) 후 살균 처리 없이 바로 발효가 가능함을 확인할 수 있었다. 발효 초기 총당 함량은 $48{\pm}2.0\;g/L$이었으며, 발효 72시간 이후 에탄올 생성 농도는 $67.4{\pm}1.4\;g/L$, 톤당 에탄올 생산량은 410.9 L (dry base)로 효소 무첨가구보다 에탄올 농도와 톤당 수득량이 각각 15%, 20% 이상 증가하였다. 이와 같은 결과는 기존의 에탄올생산 공정과 비교하여 전처리 공정에 소요되는 시간을 30-50% 이상 줄일 수 있으며, 저온 공정에 따른 에너지 사용 절감 및 초기 시설 투자비를 줄일 수 있어 바이오에탄올 생산을 위한 대체 원료로 충분한 가능성을 보여 주었다.

• 한국식품영양학회지
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• 제33권6호
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• pp.599-613
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• 2020

The purpose of this study was to evaluate the quality characteristics of white pan bread added with wheat sprout powder without treatment (WP) and wheat sprout powder with only enzyme treatment (WPE), only lactic acid bacteria treatment (WPL) and enzyme and lactic acid bacteria treatment (WPE&L). The three different powder concentration levels of 1%, 3%, and 5% were added to flour to produce the white pan bread. The bread volume and specific volume of the WPE&L group were the highest among all the addition groups. The bread weight, a-value, and b-value of the WP group was highest among all the addition groups, but the bread baking loss and the L-value of the WP group was the lowest among all the addition groups. The texture measurements indicated that the hardness, gumminess, and chewiness values of the bread were the highest in the WP group. The sensory evaluation test showed that bread in the WPE&L group with 3% wheat sprout powder was the best among all the samples studied. Based on our findings, we suggest that the enzyme and lactic acid bacteria pretreated wheat sprout powder is an effective ingredient for improving the overall quality of white pan bread.

• 한국의류학회지
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• 제32권7호
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• pp.1075-1081
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• 2008

Eco-friendly processing using enzymes has been focused in textile industry in order to reduce environmental pollutions. This paper was suggested to hydrolyze non-cellulose, such as fats and waxes in cotton fabrics by lipase treatment. Enzymatic treatment conditions were controlled according to pH, temperature, enzyme concentration, and treatment time. The physical properties of the lipase-treated cotton fabrics were evaluated by measuring weight loss, moisture regain and dyeing properties. The surface morphology of lipase-treated cotton fabrics were observed by SEM. As a result, the optimum conditions for the lipase treatment were at pH 4.2, temperature 50$^{\circ}C$, concentration 50%, and treatment time 90 minutes. Calcium chloride and Triton X-100 were effective auxiliaries in lipase treatment.

• 한국의류학회지
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• 제37권8호
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• pp.1107-1116
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• 2013

This study examined the use of cutinase and lipase to process cotton/polyester blend fabric. Optimum treatment conditions for cutinase and lipase were investigated for cotton/polyester blend fabric. The properties of enzyme-treated fabrics were evaluated and compared in optimal treatment conditions. In addition, the possibility to provide an enzymatic finishing on blend fabrics using mixed enzymes in a two-step process were studied. The weight loss of cotton/polyester blend fabrics with Triton X-100 was 0.8% and the dyeing property of blend fabrics with calcium chloride increased by a factor of 1.2. The use of two enzymes in combination with cutinase and lipase in the presence of auxiliaries resulted in a cotton/polyester blend fabric weight loss of 0.8%. In addition, the dyeing properties of cotton/polyester blend fabrics improved by a factor of 1.5 and the moisture regain of cotton/polyester blend fabrics improved by a factor of 1.16. However, no marked loss was observed in tensile strength. The surface morphology of cotton/polyester blend fabrics is modified through a two-enzyme treatment. The treatment of cotton/polyester blend fabrics with cutinase and lipase maintains cotton strength and improves the moisture regain of polyester fabrics.

• Journal of Microbiology and Biotechnology
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• 제29권11호
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• pp.1749-1759
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• 2019

Aspergillus ochraceus biofilm, developed on an inert support, can produce tannase in Khanna medium containing 1.5% (w/v) tannic acid as the carbon source, at an initial pH of 5.0, for 72 h at 28℃. Addition of 0.1% (w/v) yeast extract increased enzyme production. The enzyme in the crude filtrate exhibited the highest activity at 30℃ and pH 6.0. At 50℃, the half-life (T50) was 60 min and it was 260 min at pH 6.0. In general, addition of detergents and surfactants did not affect tannase activity significantly. Tannase has potential applications in various biotechnological processes such as the production of propyl gallate and in the treatment of tannin-rich effluents. The content of tannins and total phenolic compounds in effluents from leather treatment was reduced by 56-83% and 47-64%, respectively, after 2 h of enzyme treatment. The content of tannins and total phenolic compounds in the sorghum flour treated for 120 h with tannase were reduced by 61% and 17%, respectively. Interestingly, the same A. ochraceus biofilm was able to produce tannase for three sequential fermentative process. In conclusion, fungal biofilm is an interesting alternative to produce high levels of tannase with biotechnological potential to be applied in different industrial sectors.