• 분석과학
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• 제8권4호
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• pp.591-596
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• 1995

Enzyme multilayers composed of avidin and biotin-labeled enzymes were prepared on the surface of electrode, through a strong affinity between avidin and biotin (binding constant: ca $10^{15} M^{-1}$). The enzyme multilayers were useful for the improvement of the performance characteristies of enzyme sensors. The output current of the enzyme sensors depended linearly on the number of enzyme layers deposited. Thus, lactate oxidase (LOx) and alcohol oxidase (AlOx) were deposited after being modified with biotin for constructing enzyme sensors sensitive to L-lactate and ethanol respectively. It was also possible to deposit two different kinds of enzymes successively in a single multilayer. The glucose oxidase (GOx) and ascorbate oxidase (AsOx) were built into a multilayer structure on a Platinum electrode. The GOx, AsOx multilayer-modified electrode was useful for the elimination of ascorbic acid interference of the glucose sensor.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.52-55
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• 2000

Structually organized mono- and multilayers were developed on gold for the catalytic and affinity biosensing using hyper-branched dendrimers. For the catalytic biosensing interface, a new approach to construct a multilayered enzyme film on the electrode surface was developed. The film was prepared by layer-by-layer depositions of dendrimers and periodate-oxidized glucose oxidase. The voltammograms obtained from the GOx/dendrimer multilayered electrodes revealed that bioelectrocatalytic response is directly correlated to the number of deposited bilayers. From the analysis of voltammetric and ellipsometric signals, the coverage of active enzyme per layer during the layering steps was estimated, demonstrating the spatially-ordered multilayer formation. As an extension of the study, dendrimers having various degrees of ferrocenyl modification were prepared and used. The resulting electrodes were electrochemically characterized, and the density of ferrocenyl groups, active enzyme coverage, and sensitivity were estimated. For the affinity-sensing surrface, a biosensor system based on avidin-biotin interaction was developed. As the building block of affinity monolayer, G4 dendrimer having partial ferrocenyl-tethered surface groups was prepared and used. And the biotinylated and electroactive dendritic monolayer was used for the affinity-sensing surface interacting with avidin. Electrochemical characterization of the resulting biosensor was conducted using free enzyme in electrolyte in terms of degree of surface coverage with avidin and subsequent surface shielding.

• 한국고분자학회지:고분자과학과기술
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• 제15권3호
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• pp.260-273
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• 2004

Layer-by-layer 자기조립방법 (self-assembly method) 이라고 불리는 담지자기조립방법 (dip self-assembly method)을 이용한 다층초박막은 다양한 전기적, 자기적 성질을 갖는 물질뿐만 아니라 DNA 또는 효소 (enzyme)같은 바이오 물질들을 기판의 크기나 형태에 관계없이 각각의 층에 나노미터 두께로 삽입시킬 수 있음에 따라서 초박막안에 우리가 원하는 특정 성질을 부여할 수 있다. (중략)

• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 추계 총회 및 학술발표회
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• pp.142-145
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• 2004

Anion-exchange porous hollow-fiber membranes with a thickness of about 1.2 mm and a pore size of about $0.30{\mu}m$ were used as a supporting matrix to immobilize cycloisomaltooligosaccharide glucanotransferase (CITase). CITase was immobilized to the membrane via anion-exchange adsorption and by subsequent enzymatic cross-linking with transglutaminase, the amount of which ranged from 3 to 110 mg per g of the membrane. The degree of enzyme multilayer binding was equivalent to 0.3 to 9.8. Dextran, as the substrate, was converted into seven- to nine-glucose-membered cycloisomaltooligosaccharides (CI-7, -8, and -9) at a maxi mum yield of $28\%$ in weight at a space velocity of 10 per hour during the permeation of $2.0(w/w)\%$ dextran solution across the CITase-immobilized porous hollow-fiber membrane.

• Korean Chemical Engineering Research
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• 제60권4호
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• pp.550-556
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• 2022

당, 알코올, 유기산 및 아미노산 등과 같은 다양한 유기물에 포함된 화학에너지를 전기에너지로 전환시키는 효소 연료전지의 성능은 anode 뿐만 아니라 cathode에도 큰 영향을 받는다. 본 연구의 목적은 laccase 기반의 고성능 cathode 전극을 개발하는데 있다. 효소, 전자전달체 및 탄소나노튜브로 구성된 효소 복합체를 제조하고 이를 전극 표면에 다층으로 부착하며 층수 및 탄소나노튜브의 첨가 유무가 전극 성능에 미치는 영향을 조사하였다. 전극 표면에 효소-전자전달체(Lac-(PVI-Os-dCl))의 층수가 증가할수록 전극에서 발생되는 환원 전류량이 증가하였다. 탄소나노튜브가 첨가된 효소-전자전달체 복합체 전극(Lac-SWCNTs-(PVI-Os-dCl))이 Lac-(PVI-Os-dCl) 전극에 비하여 1.7배 많은 환원 전류를 생성하였다. Lac-SWCNTs-(PVI-Os-dCl)과 Lac-(PVI-Os-dCl)의 비율을 변화시키며 적층한 전극들에서 2층의 Lac-(PVI-Os-dCl)과 2층의 Lac-SWCNTs-(PVI-Os-dCl)으로 구성된 전극이 가장 많은 양의 환원 전류(10.1±0.1 µA)를 생성하였다. 단일 층의 Lac-(PVI-Os-dCl)로 구성된 cathode를 사용하는 셀과 최적화된 cathode를 사용하는 셀의 최대 생산 전력밀도는 각각 0.46±0.05와 1.23±0.04 µW/cm²였다. 본 연구 결과는 전극 표면에 laccase, 전자전달체 및 탄소나노튜브로 구성된 복합체의 적층 최적화를 통해 cathode 및 이를 이용하는 효소 연료전지의 성능을 향상시킬 수 있음을 시사한다.