• Title/Summary/Keyword: Environmental specimens.

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New Report of Three Unrecorded Species in Trichoderma harzianum Species Complex in Korea

  • Jang, Seokyoon;Kwon, Sun Lul;Lee, Hanbyul;Jang, Yeongseon;Park, Myung Soo;Lim, Young Woon;Kim, Changmu;Kim, Jae-Jin
    • Mycobiology
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    • v.46 no.3
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    • pp.177-184
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    • 2018
  • The genus Trichoderma (Hypocreaceae, Ascomycota) consists of globally distributed fungi. Among them, T. harzianum, one of the most commonly collected Trichoderma species, had been known as a polyphyletic or aggregate species. However, a total of 19 species were determined from the polyphyletic groups of T. harzianum. Thus, we explored Korean "T. harzianum" specimens that were collected in 2013-2014. These specimens were re-examined based on a recent study with translate elongation factor 1-alpha ($EF1{\alpha}$) sequences to reveal cryptic Trichoderma species in Korea. As a result, four different species, T. afroharzianum, T. atrobruneum, T. pyramidale, and T. harzianum, were identified. Except T. harzianum, the other three species have not been reported in Korea. In this work, we describe these species and provide figures.

A Checklist of Mushrooms of Phou Khao Khuoay National Protected Area (PKKNPA) of Lao-PDR

  • Lee, Jin Heung;Kim, Dae Ho;Yun, Ji Ho;Hong, Min Young;Lee, Jong Kyu
    • Journal of Forest and Environmental Science
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    • v.34 no.6
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    • pp.457-460
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    • 2018
  • Mushroom survey was conducted in the Phou Khao Khuoay National Protected Area (PKKNPA) located at east direction 65km from Vientiane, the capital city of Lao-PDR from September 2015 to November 2016. During this periods, mushroom specimens were collected from 10 different survey sites, and then were identified and classified into 149 species, 113 genera, and 55 families by morphological and molecular analyses. The mushrooms belongs to Ascomycota were classified into 13 species, 7 genera, 5 families, and 5 orders, while those belongs to Basidiomycota were classified into 136 species, 113 genera, 55 families, and 18 orders, respectively. Among these mushrooms, the most species-rich families were Polyporaceae (18.1%), Marasmiaceae (11.0%), Ganodermataceae (8.6%), Xylariaceae (5.8%), Russulaceae (5.4%), Agaricaceae (4.7%), Boletaceae (4.7%), Hymenochaetaceae (3.9%), and Amanitaceae (3.6%), and comprised 65.8% of the total specimens identified.

Experimental Study on Shear Retrofitting of Concrete Columns Using Iron-Based Shape Memory Alloy (철계 형상기억합금을 이용한 콘크리트 기둥의 전단보강 실험연구)

  • Jung, Donghuk;Jeong, Saebyeok;Choi, Jae-Hee;Kim, Geunoh
    • Journal of the Earthquake Engineering Society of Korea
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    • v.28 no.1
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    • pp.41-46
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    • 2024
  • The current study investigates the seismic performance of shear-dominant RC columns retrofitted with iron-based shape memory alloy (Fe SMA). Three RC columns with insufficient transverse reinforcement were designed and fabricated for lateral cyclic loading tests. Before testing, two specimens were externally confined with carbon fiber-reinforced polymer (CFRP) sheets and self-prestressed Fe SMA strips. The test results showed that both CFRP and Fe SMA performed well in preventing severe shear failure exhibited by the unretrofitted control specimen. Furthermore, the two retrofitted specimens showed ductile flexural responses up to the drift ratios of ±8%. In terms of damage control, however, the Fe SMA confinement was superior to CFRP confinement in that the spalling of concrete was much less and that the rupture of confinement did not occur.

An Experimental Study on Mortar Beam Stengthened by Composite Material (모르타르 보의 복합재료 보강 효과에 관한 실험적 연구)

  • 차승환;정일섭
    • Composites Research
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    • v.13 no.3
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    • pp.1-8
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    • 2000
  • Excellent environmental durability and handy installation procedure as well as high specific strength and stiffness have introduced fiber-reinforced polymeric composite materials into the civil and architectural engineering field. This study presents the considerably enhanced strength characteristics of the mortal beams by being reinforced with epoxy-bonded carbon fiber sheets(CFS). Three point bending and Charpy impact tests were performed on both of bare and reinforced mortar specimens. The influences of length, and the number of reinforcing plies were investigated. Strength reduction due to pre-existent notch was lessened dramatically. The acoustic emission(AE) measurement revealed the progressive damage process in reinforced specimens.

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Experimental Study on various Strength of Hardened Underwater Non-Segregation Concrete (경화된 수중불분리 콘크리트의 제강도 특성에 관한 실험적 연구)

  • 윤영수;최응규;이승훈;장일영;고용득
    • Proceedings of the Korea Concrete Institute Conference
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    • 1996.10a
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    • pp.373-378
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    • 1996
  • This paper presents the characteristics of various strengths of underwater non-segregation concrete. Three types of cements including low-heat cements has been used to make the test specimens for compressive strength, modulus of rupture and bond strength. The test specimens have been made both in ambient and underwater conditions to take into account the variation according to the environmental condition.

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Methanol fixation for scanning electron microscopy of plants

  • Ki Woo Kim
    • Applied Microscopy
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    • v.50
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    • pp.10.1-10.6
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    • 2020
  • Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

Experimental and analytical assessment of SRF and aramid composites in retrofitting RC columns

  • Dang, Hoang V.;Shin, Myoungsu;Han, Sang Whan;Lee, Kihak
    • Earthquakes and Structures
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    • v.7 no.5
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    • pp.797-815
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    • 2014
  • This research aimed to investigate retrofitting methods for damaged RC columns with SRF (Super Reinforced with Flexibility) and aramid composites and their impacts on the seismic responses. In the first stage, two original (undamaged) column specimens, designed to have a flexural- or shear-controlled failure mechanism, were tested under quasi-static lateral cyclic and constant axial loads to failure. Afterwards, the damaged column specimens were retrofitted, utilizing SRF composites and aramid rods for the flexural-controlled specimen and only SRF composites for the shear-controlled specimen. In the second stage, the retrofitted column specimens were tested again under the same conditions as the first stage. The hysteretic responses such as strength, ductility and energy dissipation were discussed and compared to clarify the specific effects of each retrofitting material on the seismic performances. Generally, SRF composites contributed greatly to the ductility of the specimens, especially for the shear-controlled specimen before retrofitting, in which twice the deformation capacity was obtained in the retrofitted specimen. The shear-controlled specimen also experienced a flexural failure mechanism after retrofitting. In addition, aramid rods moderately fortified the specimen in terms of the maximum shear strength. The maximum strength of the aramid-retrofitted specimen was 12% higher than the specimen without aramid rods. In addition, an analytical modeling of the undamaged specimens was conducted using Response-2000 and Zeus Nonlinear in order to further validate the experimental results.

Analysis of Complete Mitochondrial Genomes of Three Gadus chalcogrammus Specimens (Teleostei; Gadiformes; Gadidae) from Korea and Japan

  • Lee, Chung Il;Jung, Hae Kun;Yoo, Hae-Kyun;Kim, Hyun-Woo;Park, Hyun Je;Kang, Chang-Keun;Shim, Jeong Hee;Kim, Keun-Yong;Park, Joo Myun;Yoon, Moongeun
    • Journal of Marine Life Science
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    • v.7 no.1
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    • pp.52-54
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    • 2022
  • Mitochondrial genomes of three specimens of Gadus chalcogrammus Pallas 1,814 from Korea and Japan were completely analyzed by the primer walking method. They were 16,570~16,571 bp in length, each comprising 13 protein-coding genes, two ribosomal RNA genes, and 22 transfer RNA genes. Their gene orders were identical to those of conspecific specimens, but exhibited unique haplotypes. In the phylogenetic tree, the juvenile Korean and adult Japanese specimens were separated from the dominant clade composed of specimens from Japan, Korea, the Bering Sea, and the Arctic, including the adult Korean specimen.

Stiffening schemes for CFS built-up I-beams with large global imperfections: Capacity and behaviour

  • Dar, M. Adil;Anbarasu M.;Dar, A.R.;Islam, Naqeeb Ul;Ghowsi, Ahmad Fayeq;Carvalho, Hermes
    • Steel and Composite Structures
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    • v.42 no.4
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    • pp.447-458
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    • 2022
  • Cold-formed steel (CFS) sections are thin-walled, therefore, more susceptible to different types of geometric imperfections. Global type of geometric imperfections has a significant impact on the load-carrying capacity of flexural members. This paper reports an experimental study that discusses the influence of global imperfections on the flexural response of CFS built-up I-beams composed of two lipped channels, with simply supported ends, under four-point loading. Global imperfections of magnitude over eight times the maximum permissible ones were induced in the specimens, leading to their distress. Using various simple stiffening schemes, the capacity and stiffness of the distressed specimens were improvised. The performance comparisons were made based on the maximum loads resisted, flexural stiffnesses offered, and failure modes experienced by the specimens. As experimental data on such distressed specimens are currently lacking in the literature, the test results of the present study will provide the necessary data needed by future researchers to numerically extend this study further, which will help in the development of necessary design guidelines for the same. The stiffening schemes significantly improved the structural efficiency of distressed specimens in terms of strength and stiffness, by over 60%. As a result, an effective and time-saving solution to such realistic structural engineering problems is given.

Damage Monitoring of Concrete With Acoustic Emission Method for Nuclear Waste Storage: Effect of Temperature and Water Immersion

  • Park, June-Ho;Kwon, Tae-Hyuk;Han, Gyeol;Kim, Jin-Seop;Hong, Chang-Ho;Lee, Hang-Lo
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.20 no.3
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    • pp.297-306
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    • 2022
  • The acoustic emission (AE) is proposed as a feasible method for the real-time monitoring of the structural damage evolution in concrete materials that are typically used in the storage of nuclear wastes. However, the characteristics of AE signals emitted from concrete structures subjected to various environmental conditions are poorly identified. Therefore, this study examines the AE characteristics of the concrete structures during uniaxial compression, where the storage temperature and immersion conditions of the concrete specimens varied from 15℃ to 75℃ and from completely dry to water-immersion, respectively. Compared with the dry specimens, the water-immersed specimens exhibited significantly reduced uniaxial compressive strengths by approximately 26%, total AE energy by approximately 90%, and max RA value by approximately 70%. As the treatment temperature increased, the strength and AE parameters, such as AE count, AE energy, and RA value, of the dry specimens increased; however, the temperature effect was only minimal for the immersed specimens. This study suggests that the AE technique can capture the mechanical damage evolution of concrete materials, but their AE characteristics can vary with respect to the storage conditions.